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EBI DbfetchID U18978; SV 1; linear; genomic DNA; STD; PRO; 3503 BP. XX AC U18978; XX DT 05-MAR-1995 (Rel. 42, Created) DT 17-APR-2005 (Rel. 83, Last updated, Version 5) XX DE Thermomonospora fusca beta-1,4-exocellulase precursor gene, complete cds. XX KW . XX OS Thermobifida fusca OC Bacteria; Actinobacteria; Actinobacteridae; Actinomycetales; OC Streptosporangineae; Nocardiopsaceae; Thermobifida. XX RN [1] RP 1-3503 RX DOI; 10.1021/bi00010a030 RX PUBMED; 7880834. RA Zhang S., Lao G., Wilson D.B.; RT "Characterization of a Thermomonospora fusca exocellulase"; RL Biochemistry 34(10):3386-3395(1995). XX RN [2] RP 1-3503 RA Zhang S.; RT ; RL Submitted (21-DEC-1994) to the EMBL/GenBank/DDBJ databases. RL Sheng Zhang, Biochemistry, Molecular & Cell Biology, Cornell University, RL 460 Biotechnology Building, Ithaca, NY 14853, USA XX FH Key Location/Qualifiers FH FT source 1..3503 FT /organism="Thermobifida fusca" FT /strain="YX" FT /mol_type="genomic DNA" FT /clone="pSZ4; pSZ6" FT /db_xref="taxon:2021" FT protein_bind 249..261 FT /gene="E3" FT /bound_moiety="regulatory protein" FT 5'UTR 262..574 FT /gene="E3" FT stem_loop 349..383 FT /gene="E3" FT protein_bind 350..363 FT /gene="E3" FT /bound_moiety="regulatory protein" FT stem_loop 467..504 FT /gene="E3" FT RBS 560..564 FT /gene="E3" FT CDS 575..2365 FT /codon_start=1 FT /transl_table=11 FT /gene="E3" FT /product="beta-1,4-exocellulase precursor" FT /EC_number="3.2.1.91" FT /note="Cel C precursor" FT /db_xref="GOA:Q60029" FT /db_xref="HSSP:1EXG" FT /db_xref="InterPro:IPR017909" FT /db_xref="UniProtKB/TrEMBL:Q60029" FT /protein_id="AAA62211.1" FT /translation="MSKVRATNRRSWMRRGLAAASGLALGASMVAFAAPANAAGCSVDY FT TVNSWGTGFTANVTITNLGSAINGWTLEWDFPGNQQVTNLWNGTYTQSGQHVSVSNAPY FT NASIPANGTVEFGFNGSYSGSNDIPSSFKLNGVTCDGSDDPDPEPSPSPSPSPSPTDPD FT EPGGPTNPPTNPGEKVDNPFEGAKLYVNPVWSAKAAAEPGGSAVANESTAVWLDRIGAI FT EGNDSPTTGSMGLRDHLEEAVRQSGGDPLTIQVVIYNLPGRDCAALASNGELGPDELDR FT YKSEYIDPIADIMWDFADYENLRIVAIIEIDSLPNLVTNVGGNGGTELCAYMKQNGGYV FT NGVGYALRKLGEIPNVYNYIDAAHHGWIGWDSNFGPSVDIFYEAANASGSTVDYVHGFI FT SNTANYSATVEPYLDVNGTVNGQLIRQSKWVDWNQYVDELSFVQDLRQALIAKGFRSDI FT GMLIDTSRNGWGGPNRPTGPSSSTDLNTYVDESRIDRRIHPGNWCNQAGAGLGERPTVN FT PAPGVDAYVWVKPPGESDGASEEIPNDEGKGFDRMCDPTYQGNARNGNNPSGALPNAPI FT SGHWFSAQFRELLANAYPPL" FT sig_peptide 575..688 FT /gene="E3" FT mat_peptide 689..2362 FT /gene="E3" FT /product="beta-1,4-exocellulase" FT /EC_number="3.2.1.91" FT /note="Cel C" FT misc_feature 689..997 FT /gene="E3" FT /note="Region: cellulose binding domain" FT misc_feature 1094..2362 FT /gene="E3" FT /note="Region: catalytic domain" FT terminator 2429..2461 FT /gene="E3" FT terminator 2469..2506 FT /gene="E3" XX SQ Sequence 3503 BP; 633 A; 1272 C; 1030 G; 568 T; 0 other; cggcgatccc cctcatcatt caggtgcggt tagttccccc aggctaccga ggaccgaatt 60 tcggtccgtt tttcttgcgg cgagccctga gaccgtttcc tgttccgttc cgtcaccatc 120 cttgcgcgtc ccggcggagg ggggaagcac cccgcgagat ggctccgcca cggcctgttt 180 ccgacccccg tcacaaaagc ccatttaacg cggtatttac aaccggtcat gaagtggcta 240 ctctcttttg ggagcgctcc cgtgccgcta gtcacactgg gacgtgaatg gcgtcacggt 300 agggctcgtc gtgtgacacg cattttcgac cctgctttaa gtccctaagt gggagcgctc 360 ccagccttcg ggagaactcc cacacaacca accgtccgac gccactctcc cagcgctcaa 420 acggaggcag cagtgttcac catcccccgc tcccctccgg ggcgcccggc cgtcgtccgc 480 gcaaccacgc cgaccggtcg gctgaacact gcagcgtccg gttctcgacc atccccttgc 540 gagagaacat cctccaacca aggaagacac cgatatgagt aaagttcgtg ccacgaacag 600 acgttcgtgg atgcggcgcg gcctggcagc cgcctctgga ctggcgcttg gcgcctccat 660 ggtggcgttc gctgctccgg ccaacgccgc cggctgctcg gtggactaca cggtcaactc 720 ctggggtacc gggttcaccg ccaacgtcac catcaccaac ctcggcagtg cgatcaacgg 780 ctggaccctg gagtgggact tccccggcaa ccagcaggtg accaacctgt ggaacgggac 840 ctacacccag tccgggcagc acgtgtcggt cagcaacgcc ccgtacaacg cctccatccc 900 ggccaacgga acggttgagt tcgggttcaa cggctcctac tcgggcagca acgacatccc 960 ctcctccttc aagctgaacg gggttacctg cgacggctcg gacgaccccg accccgagcc 1020 cagcccctcc cccagccctt cccccagccc cacagacccg gatgagccgg gcggcccgac 1080 caacccgccc accaaccccg gcgagaaggt cgacaacccg ttcgagggcg ccaagctgta 1140 cgtgaacccg gtctggtcgg ccaaggccgc cgctgagccg ggcggttccg cggtcgccaa 1200 cgagtccacc gctgtctggc tggaccgtat cggcgccatc gagggcaacg acagcccgac 1260 caccggctcc atgggtctgc gcgaccacct ggaggaggcc gtccgccagt ccggtggcga 1320 cccgctgacc atccaggtcg tcatctacaa cctgcccggc cgcgactgcg ccgcgctggc 1380 ctccaacggt gagctgggtc ccgatgaact cgaccgctac aagagcgagt acatcgaccc 1440 gatcgccgac atcatgtggg acttcgcaga ctacgagaac ctgcggatcg tcgccatcat 1500 cgagatcgac tccctgccca acctcgtcac caacgtgggc gggaacggcg gcaccgagct 1560 ctgcgcctac atgaagcaga acggcggcta cgtcaacggt gtcggctacg ccctccgcaa 1620 gctgggcgag atcccgaacg tctacaacta catcgacgcc gcccaccacg gctggatcgg 1680 ctgggactcc aacttcggcc cctcggtgga catcttctac gaggccgcca acgcctccgg 1740 ctccaccgtg gactacgtgc acggcttcat ctccaacacg gccaactact cggccactgt 1800 ggagccgtac ctggacgtca acggcaccgt taacggccag ctcatccgcc agtccaagtg 1860 ggttgactgg aaccagtacg tcgacgagct ctccttcgtc caggacctgc gtcaggccct 1920 gatcgccaag ggcttccggt ccgacatcgg tatgctcatc gacacctccc gcaacggctg 1980 gggtggcccg aaccgtccga ccggaccgag ctcctccacc gacctcaaca cctacgttga 2040 cgagagccgt atcgaccgcc gtatccaccc cggtaactgg tgcaaccagg ccggtgcggg 2100 cctcggcgag cggcccacgg tcaacccggc tcccggtgtt gacgcctacg tctgggtgaa 2160 gcccccgggt gagtccgacg gcgccagcga ggagatcccg aacgacgagg gcaagggctt 2220 cgaccgcatg tgcgacccga cctaccaggg caacgcccgc aacggcaaca acccctcggg 2280 tgcgctgccc aacgccccca tctccggcca ctggttctct gcccagttcc gcgagctgct 2340 ggccaacgcc tacccgcctc tgtaaagcgg agtgaggcaa cggctgacag cctcaacgag 2400 gaactgatca gcacctccta gccggagacg gcgcccgtcc actccccgtg ggcgggcgcc 2460 gcttttatgc cgacccgtgc cccagccgca aggggcacgg gtcggcctat tccggcgatg 2520 tcggtcacgt cgccctagca cccggaaacg ccgagaaaga ctgccccgaa acggtcctct 2580 cccatccctg cattaggttg gcgcagtccg cctatggctt cgtgggccgg aacccaaccc 2640 accatcaacg agaggtatca ccatggccag tgtggtgaaa ttcaatgtgc tgacggttcc 2700 tcccggtgcc ggcgccaccc cggaggacgt ttgccaagcg cgcaggcctc gtggagaacc 2760 gggccgggtt tgaggagttc caactgctgg cgcccggcga cgggacggac aagtacatcg 2820 tctacacgcg ctggcgctcc ggagaggact accagaactg gctgaacagc gaggccttcc 2880 agcgcggaca cgcccaggcc tctgaagact cccgccgcag cagccagggc ggcccggccg 2940 cgtccgcgag tgaactctgg tccttcgaag tcgtccagca cgtccaggcc caggactgat 3000 cccggtgcgg ccctcggttc tttaccgggg gccgcccacc cccttcatcc cttttcttct 3060 cccccgcacc ccttttgatc tgcaatgatg gaatttgcga ttcttgagaa ggccgatcgt 3120 gtccatgact gcgcagaagg caggacgacc acgcgtaccg gtcgacatcg aaggagtcaa 3180 ctgacagtgg ggactatcgc ggggctgatt gtcgcgctgt caggcgtggg gatggtctcg 3240 gccaacgtgc tcccgtggga accgtcggac ccggcatccg tggtccccgc cacctcgcag 3300 ggcagcagtt ctcccatgac gccggagccc tcgcgtcccc ggtaccccca ctcgtgcgct 3360 ccgtggtcga agaggtgccc agcgcaagcg gagaactgcg ggtcgtcgaa ggtgacgggg 3420 aggtcgtcgg cgaaggcacg ctcctgcgct acctggtgga ggtcgaagaa gggcttcccg 3480 gagaccccgc cgacttcgct gca 3503 // |
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