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EBI DbfetchID U18943; SV 1; linear; genomic DNA; STD; PRO; 6656 BP. XX AC U18943; X99465; XX DT 24-JAN-1995 (Rel. 42, Created) DT 17-APR-2005 (Rel. 83, Last updated, Version 7) XX DE Bacillus stearothermophilus mannitol transport protein (MtlA), putative DE transcriptional regulator (MtlR), mannitol enzyme IIA (MtlF) and DE mannitol-1-phosphate dehydrogenase (MtlD) genes, complete cds. XX KW . XX OS Geobacillus stearothermophilus OC Bacteria; Firmicutes; Bacillales; Bacillaceae; Geobacillus. XX RN [1] RP 1-6656 RX PUBMED; 8824601. RA Henstra S.A., Tolner B., ten Hoeve Duurkens R.H., Konings W.N., RA Robillard G.T.; RT "Cloning, expression, and isolation of the mannitol transport protein from RT the thermophilic bacterium Bacillus stearothermophilus"; RL J. Bacteriol. 178(19):5586-5591(1996). XX RN [2] RP 1-6656 RA Henstra S.A.; RT ; RL Submitted (21-DEC-1994) to the EMBL/GenBank/DDBJ databases. RL Sytse A. Henstra, Biochemistry and GBB, University of Groningen, Nijenborgh RL 4, Groningen, 9747 AG, The Netherlands XX CC On Aug 7, 1996 this sequence version replaced gi:622990. XX FH Key Location/Qualifiers FH FT source 1..6656 FT /organism="Geobacillus stearothermophilus" FT /strain="ATCC 7954" FT /mol_type="genomic DNA" FT /db_xref="taxon:1422" FT RBS 348..355 FT /gene="MtlA" FT CDS 367..1782 FT /codon_start=1 FT /transl_table=11 FT /gene="mtlA" FT /product="mannitol transport protein" FT /note="mannitol enzyme IIBC" FT /db_xref="GOA:P50852" FT /db_xref="InterPro:IPR003352" FT /db_xref="InterPro:IPR003501" FT /db_xref="InterPro:IPR004718" FT /db_xref="InterPro:IPR013011" FT /db_xref="InterPro:IPR013014" FT /db_xref="UniProtKB/Swiss-Prot:P50852" FT /protein_id="AAC44463.1" FT /translation="MTHTSENQAGFRVKIQRFGSYLSGMIMPNIGAFIAWGIITALFIP FT TGWLPNETFAKLVGPMITYLLPLLIGYTGGKMIYDVRGGVVGATATMGVIVGSDIPMFL FT GAMIMGPLGGYLIKKFDQQIQGKVKQGFEMLVNNFSAGIIGGLLTLAAFKGVGPVVSAI FT SKTLAAGVEKIVDLHLLPLANIFIEPGKVLFLNNAINHGILSPLGIEQAAKTGKSILFL FT LEPNPGPGLGILLAYWLFGKGMAKQSAPGAIIIHFLGGIHEIYFPYVLMRPILILAAIA FT GGVSGVLTFTIFDAGLVAVPSPGSIFALLAMTPKGNYLGVLAGVLVATAVSFFVASIFL FT KSAKNNEEDITKATEKMQQLKGKKSDVVAVLKNEEKVIPAKVKKIVFACDAGMGSSAMG FT ASILRNKMQKAGLNIEVTNTAINQLPEDADIVITHQNLTDRAKEKLPKAFHISVENFLN FT SPKYDELIEMLKK" FT RBS 2052..2058 FT /gene="MtlR" FT CDS 2066..4159 FT /codon_start=1 FT /transl_table=11 FT /gene="MtlR" FT /note="putative transcriptional regulator" FT /db_xref="GOA:Q45419" FT /db_xref="InterPro:IPR002178" FT /db_xref="InterPro:IPR007737" FT /db_xref="InterPro:IPR011608" FT /db_xref="InterPro:IPR013011" FT /db_xref="InterPro:IPR013196" FT /db_xref="InterPro:IPR016152" FT /db_xref="UniProtKB/TrEMBL:Q45419" FT /protein_id="AAC44464.1" FT /translation="MYISARERKILDILLSNDEGTTVGELAEQLDVSNRTIHRDLKGIE FT RILKDYGLQLVKKAGVGIRVIGEEKKKKELALHLFHLSHKEYTPEERQVIILIALLEAT FT EPVKLLSLANDLNVTVATISHDLDKINQMIEKYGLSLIRKRGYGVEIAGSESAKRRMMS FT ELLFHHVDEHEFLSLMKESIQKKSIDKLNTVTEKLLGLIDKKKLVTIEQQIERIKEELP FT FTIADSSYIALVVHLALAIERISQGESINFDQQYLETIQTTKEYETAEKIARSLEHAFR FT ITIPKEEIGYITMHLMGAKLRDRQGYMLEEASFEVGIKAQELIRFVSAELHVDITNDYT FT LYEDLVVHLKPALYRIQHNMGIANPLLEKIVQDYPELFAVLEKGVKQVFPDVTVPKEEI FT GYLVLHFAAALLREKKGLRALVICSSGLGTAKILATRLKKEIPDIVHMEQISVFELRNT FT DVTRYDLIVSTVPIDGLAKPYFVVSPMLREEEVLSIKQFLKSRNAVSRKEDVDDAVARS FT HDVIKKMDSIQKLSETILRILEGFTLEQVQGHSIKEVLAEACHQLWQKGIIRNSEKVLT FT ELLRRETLGGLGIPETSLALYHTRSPAVVFPSFTIYRLTDCQTIKGMDDQPMVVNTVLL FT LLAPDSPSQEMLSVLSHISSLVIKDEDSMALFAKGDRQQVYSFLSHHLEKFFMIILNNI FT RSE" FT RBS 4147..4154 FT /gene="MtlF" FT CDS 4161..4598 FT /codon_start=1 FT /transl_table=11 FT /gene="MtlF" FT /product="mannitol enzyme IIA" FT /db_xref="GOA:Q45420" FT /db_xref="InterPro:IPR002178" FT /db_xref="InterPro:IPR016152" FT /db_xref="UniProtKB/Swiss-Prot:Q45420" FT /protein_id="AAC44465.1" FT /translation="MSMPILKKENIVLHARVENKTEAIRLAGQILVNNGYVEDSYIDKM FT FEREALTSTYMGNFIAIPHGTEDAKQFVKHSGISIIQIPDGVDFGDGNIVKLLIGIAGK FT NNEHLEILSKIAIVCSEVENVETMIKAATEEEILSILNEVN" FT RBS 4588..4592 FT /gene="MtlD" FT CDS 4601..5749 FT /codon_start=1 FT /transl_table=11 FT /gene="MtlD" FT /product="mannitol-1-phosphate dehydrogenase" FT /db_xref="GOA:Q45421" FT /db_xref="InterPro:IPR000669" FT /db_xref="InterPro:IPR008927" FT /db_xref="InterPro:IPR013118" FT /db_xref="InterPro:IPR013131" FT /db_xref="InterPro:IPR013328" FT /db_xref="InterPro:IPR016040" FT /db_xref="UniProtKB/Swiss-Prot:Q45421" FT /protein_id="AAC44466.1" FT /translation="MLAVHFGAGNIGRGFIGSLLSQSGYEVVFVDINDELVRLLKEKQE FT YRVIIADENRQELLIRNVSAVNSQTEREKVIDYITKAHLITTAVGPHILPAIATILAEG FT LQKRITINKTPLHIIACENMIGGSDVLKSHVFEKISEADKPLFEKYYGFLNCAVDRIVP FT NQKHDDPLSVVVEPFFEWVIEKRNIIGAIPPIQGAHFVDDLKPYIERKLFTVNTGHAIA FT SYLGYYKKLQTIQEAMCDQEIRSDVEKALHESGAVLVKKYGWNENEHQSYIQKIIQRFI FT NPSISDEVVRVARSPIRKLGANDRLIGPATQYYDLFGQVPHGLVKGIAALLLFDYENDE FT EAVALQKTIQETGVEGALYQYSQLEKDHPLVIAIKDQWQHLK" XX SQ Sequence 6656 BP; 2042 A; 1218 C; 1504 G; 1892 T; 0 other; gccgttccag cgccgatggc ctttttccca ttctccggtt ataaaaactc tttctacgga 60 gaccttcatg ccaacggccg ctctggagtg gagttttata cacgcaaaaa gatggtgacg 120 gtggggtact taagcaatag taggtagaaa ggactgaatc agcagactca gtcctttttt 180 gttgtatagc agtgtttggc aggtgaattg ttaaaggaga tactggcgga acttatttat 240 ttttaaaaaa tgacaataat gcattggaca aaatgatatt tacttatcgt tgaatactcc 300 gtgtaagcgc tttaagatga aaatagagaa aagataaagt ctagagaaaa gggggaaaaa 360 tgaagcatga ctcatacatc agaaaatcag gctggctttc gtgtaaagat tcagcgtttt 420 ggtagttatt taagcggaat gattatgccg aatattgggg catttattgc ttggggaata 480 attacagcgc tatttattcc aacaggatgg ctgccaaatg aaacgtttgc gaagcttgtt 540 ggcccaatga ttacgtattt attgccatta ttaattggtt atacgggcgg aaaaatgatt 600 tatgatgttc gcggcggtgt tgtcggtgcg acagcgacaa tgggagtcat tgtcggttca 660 gacattccaa tgtttcttgg cgcgatgata atggggccac taggtggtta cctaatcaaa 720 aaattcgatc agcagatcca aggtaaagtg aaacagggct ttgaaatgct tgtcaataat 780 ttttccgctg gaatcatcgg cgggctgttg acattagctg cattcaaagg ggttggaccg 840 gttgtttcgg caattagcaa aacgttagca gcaggtgtag aaaaaattgt tgatttgcac 900 ttattgccat tggcaaatat ttttattgaa ccaggaaaag tgttattcct caataacgct 960 atcaatcatg gaattttaag tccgcttggc attgaacaag cagcaaaaac aggaaaatcg 1020 attttattcc tgcttgaacc aaatcctggc ccgggccttg gtattttgtt agcatattgg 1080 ttatttggaa aaggaatggc aaagcaatcc gcgccaggag caatcatcat tcatttctta 1140 gggggaattc atgaaattta cttcccatat gttttaatga gaccgatttt aattctcgcg 1200 gcaatcgctg gcggagtgag cggggtatta acatttacca ttttcgatgc tggtcttgtg 1260 gccgttccat ccccaggaag tatttttgcc ctattagcaa tgacgccaaa aggtaattat 1320 cttggggttt tggcgggcgt attggtcgca acggcagtgt cattcttcgt cgcatcgatt 1380 ttcttaaaat cggcgaaaaa taacgaagaa gatataacaa aggcgacgga aaaaatgcaa 1440 caattaaaag ggaaaaagag cgatgttgtt gctgtattga aaaatgaaga aaaagtaatc 1500 ccagccaagg tcaaaaagat cgtattcgct tgtgatgcag gaatgggttc gagtgcgatg 1560 ggagcgtcga ttttacgtaa taaaatgcaa aaagctggat taaatattga agtaacaaat 1620 acagcaatta accaacttcc ggaagatgct gatattgtaa ttactcacca aaacttaaca 1680 gatcgcgcga aggaaaaatt gccaaaggcg tttcatatat cggtggaaaa tttcttaaac 1740 agtccgaaat atgatgagct aatcgaaatg ttaaaaaaat aaaagggtag gcacatgtag 1800 gcacatatta aaacatgact aatatgtgcc taccctttat tttttaagga tttttaggct 1860 ttaggagttg ataaacgaac gcttaccttt taatttttcg cagaaacgta ttttattaaa 1920 agatgtcaac aataaaagag acgaaaaaat aatatctatt tgttgtaatg aaaaggatcg 1980 ggacttaaaa tttttattga acaggttatt tattatttta aatcgtgtca acaatggttg 2040 cggaaggcgg gaaggtggag gggcaatgta tatatctgca cgcgagcgaa aaatactaga 2100 tattttgttg tcaaacgacg aaggaacgac ggtaggagaa cttgccgagc aactagacgt 2160 cagcaaccgt accatccacc gggatttaaa agggatcgag cgcattttaa aggattacgg 2220 cctgcaactt gtcaaaaaag caggggtcgg cattcgggtt attggagaag aaaaaaagaa 2280 aaaagagcta gctcttcatc tatttcattt gtcacataag gaatatacac cagaggaacg 2340 acaagtaatc attctgattg ctttgctgga agcaacggag cctgtcaagc ttttatcgct 2400 tgcaaacgac ctcaatgtta ctgtcgcaac cattagccat gatttagata aaatcaatca 2460 aatgatagaa aaatacggac tctccttaat ccgaaaaaga ggatatggtg tggaaattgc 2520 tggatctgag tccgcaaagc ggagaatgat gagtgaacta ctatttcatc atgtggatga 2580 acatgagttt ctctcgttaa tgaaagagtc gattcaaaaa aagtccatcg ataaattgaa 2640 tacggttaca gaaaaacttc tcgggctaat cgataagaaa aaattagtca ccattgaaca 2700 acaaattgaa cggatcaagg aagagcttcc ttttactatt gccgacagtt cgtatattgc 2760 gttagtggtc catcttgcct tagcaataga gcgaatcagc caaggggaat cgattaattt 2820 tgatcaacaa tatttggaaa cgattcagac aacaaaagaa tacgaaaccg cagaaaaaat 2880 tgctcgatcg ttagaacatg cttttcgcat cacgattcca aaagaggaga tcgggtatat 2940 tacgatgcat ttaatgggag cgaaacttcg cgaccgccag ggttatatgc tagaagaggc 3000 aagttttgaa gtaggcatca aagcacaaga gctaattcgg tttgtcagtg ctgaactgca 3060 tgtagatatt acaaacgatt atacgttata cgaagatttg gtcgttcatt taaagccagc 3120 actttatcgc atccagcata acatggggat tgccaatccg cttttagaaa aaattgttca 3180 agattatccg gaactttttg cggtattgga aaaaggagta aaacaagtat ttccagatgt 3240 aaccgttcca aaggaggaaa tcggttattt agttctccac tttgcggcag cattattaag 3300 ggaaaagaaa ggattgcgtg cgttagtgat ctgctcgagt ggactaggga cggcaaaaat 3360 attagcaaca agattgaaaa aagaaattcc cgatattgta cacatggaac agatttctgt 3420 ttttgaattg agaaacacgg atgtaacgcg atacgacttg attgtttcga ccgtcccaat 3480 tgatggcttg gcaaaaccat attttgttgt cagtcccatg ctaagggaag aagaggttct 3540 gtcgattaaa cagtttttaa aaagcagaaa tgcggtttcc cgtaaggaag atgtagacga 3600 cgcggtagca agatctcatg atgtgatcaa gaaaatggat tccattcaaa aattgagcga 3660 aaccattctc cgaattttgg aagggtttac gttagaacag gtacaaggcc attccatcaa 3720 agaagtatta gcagaagcat gccatcagtt atggcaaaaa gggattattc gtaattctga 3780 gaaagtgctt acagaattgt tgcggagaga aacattaggt ggattgggaa taccggaaac 3840 gtcgcttgct ttgtaccata ctcgaagccc tgcggtggtt tttccgtctt ttacgattta 3900 taggttaacg gactgccaaa ccatcaaagg aatggacgat cagccgatgg ttgtgaatac 3960 cgttttgctg ctgttagcgc ccgattcacc tagtcaggaa atgctttctg tacttagcca 4020 cattagctca ttagtcatta aagatgaaga tagcatggca ttatttgcaa aaggggatcg 4080 ccaacaggtt tattcgtttt taagccatca tttagagaag ttttttatga ttatcttaaa 4140 caacataagg agtgaatgac atgtcaatgc caattttaaa gaaagaaaat attgtattac 4200 atgcgcgagt agaaaataaa acggaagcga ttcgcttagc aggacaaatt ttggtgaata 4260 acggttatgt agaagattct tatattgata aaatgtttga gcgggaagcg ttaacatcca 4320 catatatggg aaattttatt gctattccac atggcacaga ggatgcgaaa caatttgtca 4380 aacattctgg tatttcgatt atacaaattc ctgatggagt tgattttgga gatggcaata 4440 ttgtgaaact attgattgga atagctggaa aaaataacga acatttggaa attctctcga 4500 agatcgccat cgtttgttcg gaagtggaaa atgtagaaac gatgattaaa gcggcgacag 4560 aagaagaaat tcttagcatt ctaaatgagg tgaattaagc atgttagcag ttcatttcgg 4620 tgctggaaat atcggaagag gatttatcgg gagtttgctt tcacagtcgg gctatgaagt 4680 agtgtttgtg gatattaatg atgaattggt tcgtttatta aaggaaaaac aggagtatcg 4740 tgtgatcatt gccgatgaaa atagacaaga actgctgatt cgcaacgttt ccgctgtcaa 4800 tagtcaaacg gaacgtgaaa aggtcattga ctatatcaca aaggctcatc ttattacaac 4860 ggcggtcggg ccgcatattt taccggccat cgccacgatt cttgctgagg ggctgcaaaa 4920 aagaatcacg atcaataaaa caccgcttca tattattgct tgtgaaaata tgatcggtgg 4980 aagtgatgtg ttaaaaagcc atgtctttga aaagatttct gaagcagata aaccgttgtt 5040 cgaaaagtat tacggttttc tgaattgtgc ggtagaccgt atcgttccaa atcaaaaaca 5100 cgacgatcct ctctccgttg tggtcgagcc atttttcgaa tgggtgatcg aaaagagaaa 5160 tattattggt gctatcccgc cgattcaagg agctcatttt gttgatgatt taaagcctta 5220 tattgaacgc aagcttttta ccgtaaatac cgggcatgcg attgccagct acttaggata 5280 ctataaaaaa ttacaaacga tacaagaagc aatgtgtgat caagaaatac gttcggacgt 5340 ggaaaaagcg ctgcatgaat caggtgcggt gttagtaaag aaatatggat ggaatgaaaa 5400 cgaacatcaa tcctatattc aaaaaatcat tcaacgcttt ataaatcctt ccatctcaga 5460 tgaagtagtc cgtgttgctc gttctccaat tcgcaaactt ggggcgaatg accgtcttat 5520 tggaccggct acgcaatatt atgacttgtt cggacaagta ccacatggtt tagtcaaagg 5580 aattgccgcg ttgttattgt ttgattatga gaacgacgag gaagcagttg cattgcaaaa 5640 aaccattcaa gaaacaggag tagaaggggc tctataccaa tattcacaat tagaaaaaga 5700 tcatcctctt gttatagcaa taaaagatca atggcagcat ttaaagtaaa cggatgacga 5760 ggctgtccca aaaggtcggt aaaacgacct tttgaggaca gcctcctgct tttgttttta 5820 cattatttgt ctatttttta ctttcttaaa ggatctgctt cttcttatcc ccgtatgtcc 5880 tccctttttc tttttattgg gtggacggtg ggcgcccact ttctcaaatt gtgggcagca 5940 caaataagcg ccaatccacg gtaatttttg ggagcccctt aacaaaaagc gctggaattg 6000 ctggtatgct tgatttgccc aaaacaccgg ctcgttttcg atttgccgtc tcgatatgtc 6060 gccgcccctt cttccgtgac agccgttccc gaatctcctg ccgttgttgt tgattcttca 6120 aagaaacgcg gatggttttc gtgtctttgc ctttggcgca tgccgtttga aacgggcatc 6180 cggcacatgc cgtacaacga tacgtccgtt tgacggtaac gtacccattg tcggtcgttt 6240 ccttccgttc atacacaaac accaaccgtt cccctttagc gcaaatccac tcatccagct 6300 cctcatcata cgtcatgttc tcgattcggc cggtctcttt cgcccacgct ttcgtttgtt 6360 cccggtccag cgtgttgtac ttgatcaggg cgacgatctt ctttttctcg cagtacgtgt 6420 agttctcctc actcccatag gcggaatccg caatggcccg tttgggcatt gggcgtccat 6480 aggcggccaa ttgctccaaa tgcggaatga aacatccggc atcccccgcc cgttgatgca 6540 cgctaaatcc agtgatgaat tggttctctg tcccaatctg cacattatat cccggtttga 6600 gctggccgtt tttcatatgg tcgtctttca ttcgcatgaa tgtcgcatcc ggatcc 6656 // |
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