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EBI DbfetchID U17226; SV 1; linear; genomic DNA; STD; PRO; 5895 BP. XX AC U17226; XX DT 04-MAY-1995 (Rel. 43, Created) DT 28-JUL-2005 (Rel. 84, Last updated, Version 7) XX DE Sinorhizobium meliloti carbon flux regulator (aniA), beta-ketothiolase DE (phbA), and acetoacetyl CoA reductase (phbB) genes, complete cds. XX KW . XX OS Sinorhizobium meliloti OC Bacteria; Proteobacteria; Alphaproteobacteria; Rhizobiales; Rhizobiaceae; OC Sinorhizobium/Ensifer group; Sinorhizobium. XX RN [1] RP 1-5895 RX PUBMED; 7582015. RA Tombolini R., Povolo S., Buson A., Squartini A., Nuti M.P.; RT "Poly-beta-hydroxybutyrate (PHB) biosynthetic genes in Rhizobium meliloti RT 41"; RL Microbiology 141(Pt 10):2553-2559(1995). XX RN [2] RP 1-5895 RX DOI; 10.1007/s002030000171 RX PUBMED; 10985741. RA Povolo S., Casella S.; RT "A critical role for aniA in energy-carbon flux and symbiotic nitrogen RT fixation in Sinorhizobium meliloti"; RL Arch. Microbiol. 174(1-2):42-49(2000). XX RN [3] RP 1-5895 RA Tombolini R.; RT ; RL Submitted (16-NOV-1994) to the EMBL/GenBank/DDBJ databases. RL Riccardo Tombolini, University of Padova, Cribi Biotechnological Centre, RL Via Trieste, 75, Padova, PD 35121, Italy XX FH Key Location/Qualifiers FH FT source 1..5895 FT /organism="Sinorhizobium meliloti" FT /strain="41" FT /mol_type="genomic DNA" FT /db_xref="taxon:382" FT gene complement(2040..2630) FT /gene="aniA" FT CDS complement(2040..2618) FT /codon_start=1 FT /transl_table=11 FT /gene="aniA" FT /product="carbon flux regulator" FT /function="regulation of reserve polymer biosynthesis" FT /note="similar to ORF4 in Chromatium vinosum, PIR Accession FT Number S29277" FT /db_xref="InterPro:IPR012909" FT /db_xref="UniProtKB/TrEMBL:Q52886" FT /protein_id="AAA90981.1" FT /translation="MGKNEGQIVIKKYANRRLYNTGTSTYVTLDDLAVMVKRGEDFIVQ FT DAKSGEDITHSVLTQIIFEQESKTGNTLLPISFLRQLISFYGDQMQMVVPSYLEHSMQA FT FTEQQAQMREQITKAFGDTPIGKNLKVPLQLVEEQVRRNTEMFHQAMQMFSPFMAAPPA FT KETRKAEAKDIDELKEQLRALQTKLDNLG" FT RBS complement(2625..2630) FT /gene="aniA" FT gene 2829..4022 FT /gene="phbA" FT RBS 2829..2833 FT /gene="phbA" FT CDS 2841..4022 FT /codon_start=1 FT /transl_table=11 FT /gene="phbA" FT /product="beta-ketothiolase" FT /EC_number="2.3.1.9" FT /db_xref="GOA:P50174" FT /db_xref="HSSP:1DLU" FT /db_xref="InterPro:IPR016038" FT /db_xref="UniProtKB/Swiss-Prot:P50174" FT /experiment="experimental evidence, no additional details FT recorded" FT /protein_id="AAA90982.1" FT /translation="MSNPSIVIASAARTAVGSFNGAFGNTLAHELGAAAIKAVLERAGV FT EAGEVDEVILGQVLPAGEGQNPARQAAMKAGLPQEKTAWGMNQLCGSGLRAVALGMQQI FT ATGDAKVIVAGGMESMSMAPHCAHLRGGVKMGDYKMIDTMIKDGLTDAFYGYHMGITAE FT NVARKWQLTREEQDEFALASQNKAEAAQKAGRFADEIVPFVVKTRKGDVNVDQDEYIRH FT GATLDSIAKLRPAFDKEGTVTAGNASGLNDGAAAALLMTEAEAARRGIQPLARIVSWAT FT AGVDPQIMGTGPIPASRKALEKAGWSVADIELVEANEAFAAQACAVNKDLGWDPSIVNV FT NGGAIAIGHPIGASGARVLNTLLFEMKRRGVSKGLATLCIGGGMGVAMCVERL" FT gene 4130..4865 FT /gene="phbB" FT RBS 4130..4134 FT /gene="phbB" FT CDS 4140..4865 FT /codon_start=1 FT /transl_table=11 FT /gene="phbB" FT /product="acetoacetyl CoA reductase" FT /EC_number="1.1.1.36" FT /db_xref="GOA:P50205" FT /db_xref="HSSP:1G0N" FT /db_xref="InterPro:IPR016040" FT /db_xref="UniProtKB/Swiss-Prot:P50205" FT /experiment="experimental evidence, no additional details FT recorded" FT /protein_id="AAA90983.1" FT /translation="MSRVALVTGGSRGIGAAICVALKAAGYKVAANYAGNDERAKAFEQ FT ESGIPVYKWDVSSYQACVDGIARVEADLGPVDILVNNAGITRDAMFHKMTPEQWGEVIG FT TNLTGVFNMTHPLWSGMRDRGFGRIVNISSINGQKGQMGQVNYSAAKAGDLGLTKALAQ FT EGAAKGITVNAICPGYIGTEMVRAVPEKVLNERIIPQIPVGRLGEPEEVARCVVFLASD FT DAGFITGSTISANGGQYFA" FT terminator 4889..4908 FT /note="potential transcription termination signal" XX SQ Sequence 5895 BP; 1093 A; 1769 C; 1878 G; 1155 T; 0 other; ctgcagagcc ttgccttttg cgagaaaggc cgttcaactt tcgctcgcca tcgcatatga 60 gagggtgaat gggacaagag gcgggcggaa aagaggtgga cggacggggc gtggagatcg 120 tttcggaaga acgcgaggag gcggagttgc ccgccggtcg ctggacggcc acgcgcagga 180 cctggcgctc gcggcggcgt cggctcgttt ttatcgtcct ctcagttctc atcctcccct 240 atgccctgat cggcctctat ctcttggagt tcattcaccc ggtttcgaca ctgatgctgc 300 gcgatctcgt gctgctgcgc ggctatgacc ggcagtgggt ggaattcgac gacatcgcgc 360 cggtgctcgt ccagtcggtg atgatgtcgg aggacgggca gttctgcgcc catgccggca 420 tcgattgggc gcagatgcgc ggcgtcgtcg aggatgcgct cgacggcgaa cagacgcgcg 480 gcgccagcac gatcccgatg cagacggtca agaacctgtt cctctggaac ggccgttcct 540 tcgtgcgcaa ggcgatggaa ctaccgctcg cgatcgcggc cgatttcgcc tggagcaagc 600 ggcgcctgat ggagatctac ctgaacgtcg ccgaatgggg cgagggcata tacgggatcg 660 aggccgccgc ccggcatcat ttcggcgttt cggcggcgaa gctttcgcgc cgccaggccg 720 ctctcctcgc cgtctcgctg cccaacccga tcgatcgcac tgccggcaag ccggggcgtg 780 gcctgcggcg tctcgctgcg gtgatcgaac gccgggccgg ccgctccggc ggttatatca 840 cgtgccttta tgattaggat gaaaccaatt cattggttcg ccggccgctg atgtggcaag 900 gctgcgtttg tatctcgcat gagcgaaccc agtcggagac aggatcatgg ccaagctcgt 960 cctctatgtc ggcaacaaga actattcgtc ctggtcgctg cgggcctggc tcgccctcac 1020 ggccggcggg aattctccgt tcgaggacgt cgtccgttcg aggacgtcgt cgttcccttc 1080 gattttgcgg ccggaaatcc gcgttttcac gaaatctcgc ccacgggccg cgttcccgtt 1140 cttcatcacg gcgagatccg catatgggaa tcgctcgcga tcatcgaata tgtcgcggag 1200 cttttcccgg atgcgggtct ctggccggag gaccgcgcgg atcgtgcccg tgcgcgcagc 1260 tactccatgg agatgctctc cggcttccgg gcgctgtgcg gggcctgccc gatgaacatc 1320 cgccgtccga gaaaggcgat cgctctgtcg gacggcgttc gtcgaggacg tggcgcggat 1380 cgagacgatc tggcgggagt cggtcgcaca atcagggggg ccgttccttt tcggccggtt 1440 caccgcggcc gacgcgatgt tcgccccggt cgtcaaccgt ttcgagacct atgagctcgt 1500 ggcggacccg gcgacgctcg cctatattga cgccgtccaa gggcgcatcc ggccttccgc 1560 caatgggagg aggcggcgag agtcgaaaca tggatcgtac ccgaggacga gtcctgaggc 1620 ggatcggggc ttcccgcgcc cgagtcgaaa atttgcccgc ggggactggt caaagtgcgg 1680 ttcgacatgt ataagcgcgc gaaattccga gatcgacatc tgtttgaccc ggccattttc 1740 ggctgctgaa cagtgttttg cccgataagt ggagaatgaa atggctgtac cgaaaagaaa 1800 aacgagcccg tccaagcgtg gcatgcgccg ttccgctgac gccctcaagg ctccgaccta 1860 catcgaagac aagaactccg gcgaactgcg ccgcccgcat cacatcgacc tgaagaccgg 1920 catgtatcgc ggccgccagg tcctgacgcc gaaggaaaac gcataagcgt ttccgcctgc 1980 ggatgaagtt cgaagggtcg gcattgccgg ccctttttct ttggtggatc gaggggtgat 2040 cagcccagat tatccagttt cgtctgaagg gcgcggagct gttccttcag ttcgtcgatg 2100 tccttcgcct cggccttcct ggtttccttg gccggcgggg cggccatgaa gggcgaaaac 2160 atctgcatgg cctgatggaa catttcagtg ttgcgccgga cctgttcctc gacgagctgc 2220 agcggcacct tcaggttctt gccgatcggc gtatcgccga aggccttggt gatctgctcc 2280 cgcatctgcg cctgctgttc ggtgaaggcc tgcatcgagt gctcgagata gctcggtacg 2340 accatctgca tctggtcgcc ataaaaggag atcaactggc ggagaaagga aatcggcagg 2400 agcgtgttgc cggtcttcga ctcctgttcg aagatgatct gcgtcagcac ggaatgggtt 2460 atatcttcgc cggacttggc atcctgaact atgaagtcct cgcccctctt caccatgaca 2520 gccaggtcgt cgagggtcac gtaagtgctc gtaccggtat tgtagagtcg ccggttcgcg 2580 tatttcttga taacgatctg gccttcgttt ttccccatca gggtctcctc ttacccatgg 2640 tcccgtcttt attgtatttt tcagagtatc cgcaaaagag cgccactgac aatctctttg 2700 tgcaatgcgg cgacgcaggt gcgaaggagc gccgacaagc gaccgtgaag cttttgcggc 2760 agtgcaacta gccgtttgac tcgcgctcct ccctttgcca gtctgcctcc atcaggcaaa 2820 gcaaatatga ggaacgtccc atgagcaatc cctcgatcgt catcgccagc gcggctcgca 2880 cggccgtcgg ctccttcaac ggcgccttcg gcaatactct cgcgcacgaa ctgggtgcgg 2940 ccgccatcaa ggcggtgctg gagcgggctg gcgtcgaagc gggcgaggtg gacgaggtga 3000 tcctcggtca ggtgctgccg gcgggcgagg ggcagaatcc tgcgcggcaa gcggcgatga 3060 aggccggtct cccgcaggaa aagaccgcct ggggcatgaa ccagctttgc ggctcgggcc 3120 tgcgcgccgt agcgctcggc atgcagcaga tcgcaaccgg cgatgcgaag gtcatcgttg 3180 ccggcggcat ggagtcgatg tcgatggcgc cgcattgcgc gcacctgcgc ggcggcgtga 3240 agatgggcga ctacaagatg atcgacacga tgatcaagga cggcctgacg gatgccttct 3300 acggctacca catgggcatc accgccgaga acgttgcgcg gaaatggcag ctgacgcgcg 3360 aggaacagga cgaattcgcg cttgcctccc agaacaaggc ggaagcggcc cagaaggccg 3420 gccgtttcgc cgacgagatc gtgcctttcg tcgtgaagac gcgcaagggc gacgtaaacg 3480 tggatcagga cgagtacatc cgccacggtg ccacgctgga ttcgatcgca aagctccgcc 3540 cggccttcga caaggaaggt actgtgaccg cgggcaacgc ttcgggtctc aacgacggtg 3600 ccgccgcggc gctcttgatg accgaggccg aggcggcccg gcgcggcatc cagccgcttg 3660 cccgcatcgt ctcctgggcc acggcaggcg tcgacccgca gatcatgggc accggcccca 3720 tccccgcatc gcgcaaggcc ctcgaaaagg ccggctggtc ggtcgccgat atcgagctcg 3780 tggaggcgaa cgaggctttc gcggctcagg cctgcgccgt caacaaggac ctcggctggg 3840 atccttcgat cgtcaacgtc aatggcggag cgatcgccat cggccatccg atcggtgcct 3900 ccggtgcccg cgtgctgaac acgcttcttt tcgaaatgaa gcggcgcggc gtctccaagg 3960 ggcttgccac cctgtgcatc ggcggcggca tgggcgtcgc catgtgcgtg gaacgcctgt 4020 aaccggcctc ccgcgatgcg ggcaagtttc ggatcagatg atgcacatca gcggcccggg 4080 ctcgccgggc tgcaggggag tgcttttgca aatttgactt aaccgagtgg gaggcgagta 4140 tgagcagggt agcactggta acgggcggat cccgcggcat tggcgctgcg atttgcgtgg 4200 cgctgaaggc tgcgggctac aaggtggccg caaactatgc cggaaatgac gagagggcca 4260 aggccttcga gcaggaaagc ggcattcccg tctacaaatg ggacgtatcg agctatcagg 4320 cctgcgtcga tggcatcgcc agggtcgagg ccgacctcgg accggtcgac atcctcgtca 4380 acaatgccgg catcacccgt gacgccatgt tccacaagat gacgccggaa cagtggggcg 4440 aagtgatcgg caccaatctc accggcgtct tcaacatgac gcatccgctg tggtcgggca 4500 tgcgcgaccg cggcttcggc cgtatcgtca acatctcgtc gatcaacggc cagaaggggc 4560 agatgggcca ggtgaactat tccgccgcca aggccggcga tctcggcttg accaaggcgc 4620 tggcccagga aggggcggcg aaagggatca ccgtcaacgc gatttgcccc ggctatatcg 4680 gcaccgagat ggtgcgcgcc gttccggaaa aggtgctcaa cgagcggatc attccccaga 4740 tacccgtcgg acgcctcggc gagccggagg aagtggcacg ctgcgtcgtg tttctcgctt 4800 ccgacgacgc gggcttcatc accggctcga cgatttcggc caatggcggc cagtacttcg 4860 cctgaggcat ggtgccctcg gctttccggc gcccttcggg gcgccggatt cgtttcggcc 4920 ctggcactag atattgtggt gaacgaaagg ggaaaacgtt cccgccggcg agtcggcgtt 4980 gctctgcccc ggcaggaggc gggtgcctgt gaagcgattc ctatttccgg actcggccga 5040 tcggcagcga atcccgttaa aatttttcaa tctttccaga ctcttggatt cggaatcctt 5100 tgcgtggtca acgtccgcaa tgacgccaaa cgaagccgat cggcaacttc tgaggggtga 5160 tcctcccttg gattcagcat atggtgcgta actagatgtt gaaatctata tatagccctg 5220 aacataaagt gaatcagcgg gagtcagcga ttcgtctctg attcgttccg tcgctgttcc 5280 agaatcctga acgagagttc taaggggagg cgcaagatat ggttgccgat tggcgtattt 5340 attccccgtg gacccttgcg tttggcacgt cgcataggca tctgtttccc gccggagcat 5400 gaaagcgcgc cggctctcag tgcgtgttcc ataaatcgga gccgctttta cggataatac 5460 atacggaaaa tcagcactgc agcgagcttc gcgcgaccaa aagaagtgcg gcgctgcaga 5520 gactgcccgg gatcgagacc ttgtcctttc aatccatgat cctgagcggc cgcggcgtga 5580 ccgcggtgct cggacccacc aacaccggca agacccatta tgcgatcgag cgcatgatcg 5640 cgcatgacag cggcgtcata gggcttccgc tgagattgct cgcgcgcgag gtctacaccc 5700 gtctcgtgga gaaggtcggt caccacaatg tcgcgctgat cacgggcgag gaaaagatcg 5760 cgccgcatcg cgcccgttac tcggtctgca cggtggaggc gatgccgcgc gagacgacgg 5820 cctccttcgt ggcgatcgac gaggttcagc tcgcaggcga tctcgaacgc ggccacatct 5880 tcacggaccg gatcc 5895 // |
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