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EBI DbfetchID U17110; SV 1; linear; genomic DNA; STD; PRO; 4993 BP. XX AC U17110; XX DT 16-NOV-1995 (Rel. 45, Created) DT 17-APR-2005 (Rel. 83, Last updated, Version 6) XX DE Clostridium acetobutylicum crotonase (crt), putative butyryl-CoA DE dehydrogenase (BCD), putative a-subunit of electron-transfer flavoprotein DE (etfA), putative b-subunit of electron-transfer flavoprotein (etfB), and DE 3-hydroxybutyryl-CoA dehydrogenase (hbd) genes, complete cds. XX KW . XX OS Clostridium acetobutylicum OC Bacteria; Firmicutes; Clostridia; Clostridiales; Clostridiaceae; OC Clostridium. XX RN [1] RP 1-4993 RX PUBMED; 8655474. RA Boynton Z.L., Bennett G.N., Rudolph F.B.; RT "Cloning, sequencing, and expression of clustered genes encoding RT beta-hydroxybutyryl-coenzyme A (CoA) dehydrogenase, crotonase, and RT butyryl-CoA dehydrogenase from Clostridium acetobutylicum ATCC 824"; RL J. Bacteriol. 178(11):3015-3024(1996). XX RN [2] RP 1-4993 RA Boynton Z.L.; RT ; RL Submitted (11-NOV-1994) to the EMBL/GenBank/DDBJ databases. RL Zhuang L. Boynton, Biochemistry and Cell Biology, Rice University, 6100 RL South Main, Houston, TX 77030, USA XX FH Key Location/Qualifiers FH FT source 1..4993 FT /organism="Clostridium acetobutylicum" FT /strain="ATCC 824" FT /mol_type="genomic DNA" FT /clone="pBCS10" FT /db_xref="taxon:1488" FT -35_signal 64..72 FT -10_signal 89..94 FT CDS 178..963 FT /codon_start=1 FT /transl_table=11 FT /gene="crt" FT /product="crotonase" FT /db_xref="GOA:P52046" FT /db_xref="HSSP:1MJ3" FT /db_xref="InterPro:IPR018376" FT /db_xref="UniProtKB/Swiss-Prot:P52046" FT /protein_id="AAA95967.1" FT /translation="MELNNVILEKEGKVAVVTINRPKALNALNSDTLKEMDYVIGEIEN FT DSEVLAVILTGAGEKSFVAGADISEMKEMNTIEGRKFGILGNKVFRRLELLEKPVIAAV FT NGFALGGGCEIAMSCDIRIASSNARFGQPEVGLGITPGFGGTQRLSRLVGMGMAKQLIF FT TAQNIKADEALRIGLVNKVVEPSELMNTAKEIANKIVSNAPVAVKLSKQAINRGMQCDI FT DTALAFESEAFGECFSTEDQKDAMTAFIEKRKIEGFKNR" FT CDS 977..2116 FT /codon_start=1 FT /transl_table=11 FT /gene="bcd" FT /note="putative butyryl-CoA dehydrogenase" FT /db_xref="GOA:P52042" FT /db_xref="HSSP:1BUC" FT /db_xref="InterPro:IPR013764" FT /db_xref="UniProtKB/Swiss-Prot:P52042" FT /protein_id="AAA95968.1" FT /translation="MDFNLTREQELVRQMVREFAENEVKPIAAEIDETERFPMENVKKM FT GQYGMMGIPFSKEYGGAGGDVLSYIIAVEELSKVCGTTGVILSAHTSLCASLINEHGTE FT EQKQKYLVPLAKGEKIGAYGLTEPNAGTDSGAQQTVAVLEGDHYVINGSKIFITNGGVA FT DTFVIFAMTDRTKGTKGISAFIIEKGFKGFSIGKVEQKLGIRASSTTELVFEDMIVPVE FT NMIGKEGKGFPIAMKTLDGGRIGIAAQALGIAEGAFNEARAYMKERKQFGRSLDKFQGL FT AWMMADMDVAIESARYLVYKAAYLKQAGLPYTVDAARAKLHAANVAMDVTTKAVQLFGG FT YGYTKDYPVERMMRDAKITEIYEGTSEVQKLVISGKIFR" FT CDS 2135..2893 FT /codon_start=1 FT /transl_table=11 FT /gene="etfB" FT /note="putative b-subunit of electron-transfer FT flavoprotein" FT /db_xref="GOA:P52040" FT /db_xref="HSSP:1O94" FT /db_xref="InterPro:IPR014729" FT /db_xref="UniProtKB/Swiss-Prot:P52040" FT /protein_id="AAA95969.1" FT /translation="MNIVVCLKQVPDTAEVRIDPVKGTLIREGVPSIINPDDKNALEEA FT LVLKDNYGAHVTVISMGPPQAKNALVEALAMGADEAVLLTDRAFGGADTLATSHTIAAG FT IKKLKYDIVFAGRQAIDGDTAQVGPEIAEHLGIPQVTYVEKVEVDGDTLKIRKAWEDGY FT EVVEVKTPVLLTAIKELNVPRYMSVEKIFGAFDKEVKMWTADDIDVDKANLGLKGSPTK FT VKKSSTKEVKGQGEVIDKPVKEAADMLSQN" FT CDS 2931..3944 FT /codon_start=1 FT /transl_table=11 FT /gene="etfA" FT /note="putative a-subunit of electron-transfer FT flavoprotein" FT /db_xref="GOA:P52039" FT /db_xref="HSSP:1O97" FT /db_xref="InterPro:IPR014731" FT /db_xref="UniProtKB/Swiss-Prot:P52039" FT /protein_id="AAA95970.1" FT /translation="MNKADYKGVWVFAEQRDGELQKVSLELLGKGKEMAEKLGVELTAV FT LLGHNTEKMSKDLLSHGADKVLAADNELLAHFSTDGYAKVICDLVNERKPEILFIGATF FT IGRDLGPRIAARLSTGLTADCTSLDIDVENRDLLATRPAFGGNLIATIVCSDHRPQMAT FT VRPGVFFEKLPVNDANVSDDKIEKVAIKLTASDIRTKVSKVVKLAKDIADIGEAKVLVA FT GGRGVGSKENFEKLEELASLLGGTIAASRAAIEKEWVDKDLQVGQTGKTVRPTLYIACG FT ISGAIQHLAGMQDSDYIIAINKDVEAPIMKVADLAIVGDVNKVVPELIAQVKAANN" FT CDS 4088..4936 FT /codon_start=1 FT /transl_table=11 FT /gene="hbd" FT /product="3-hydroxybutyryl-CoA dehydrogenase" FT /db_xref="GOA:P52041" FT /db_xref="HSSP:3HDH" FT /db_xref="InterPro:IPR016040" FT /db_xref="UniProtKB/Swiss-Prot:P52041" FT /protein_id="AAA95971.1" FT /translation="MKKVCVIGAGTMGSGIAQAFAAKGFEVVLRDIKDEFVDRGLDFIN FT KNLSKLVKKGKIEEATKVEILTRISGTVDLNMAADCDLVIEAAVERMDIKKQIFADLDN FT ICKPETILASNTSSLSITEVASATKTNDKVIGMHFFNPAPVMKLVEVIRGIATSQETFD FT AVKETSIAIGKDPVEVAEAPGFVVNRILIPMINEAVGILAEGIASVEDIDKAMKLGANH FT PMGPLELGDFIGLDICLAIMDVLYSETGDSKYRPHTLLKKYVRAGWLGRKSGKGFYDYS FT K" XX SQ Sequence 4993 BP; 1898 A; 593 C; 1069 G; 1433 T; 0 other; agtacggtaa tgttatttaa atatatataa aaattattaa aatttaatat aaaatggttt 60 taatattgaa atataaaata aatcattata taataattat agaagcatat gcttctaatt 120 ttttgcccgt ctttgttata atattaacaa taaaaaaata ttttaggagg attagtcatg 180 gaactaaaca atgtcatcct tgaaaaggaa ggtaaagttg ctgtagttac cattaacaga 240 cctaaagcat taaatgcgtt aaatagtgat acactaaaag aaatggatta tgttataggt 300 gaaattgaaa atgatagcga agtacttgca gtaattttaa ctggagcagg agaaaaatca 360 tttgtagcag gagcagatat ttctgagatg aaggaaatga ataccattga aggtagaaaa 420 ttcgggatac ttggaaataa agtgtttaga agattagaac ttcttgaaaa gcctgtaata 480 gcagctgtta atggttttgc tttaggaggc ggatgcgaaa tagctatgtc ttgtgatata 540 agaatagctt caagcaacgc aagatttggt caaccagaag taggtctcgg aataacacct 600 ggttttggtg gtacacaaag actttcaaga ttagttggaa tgggcatggc aaagcagctt 660 atatttactg cacaaaatat aaaggcagat gaagcattaa gaatcggact tgtaaataag 720 gtagtagaac ctagtgaatt aatgaataca gcaaaagaaa ttgcaaacaa aattgtgagc 780 aatgctccag tagctgttaa gttaagcaaa caggctatta atagaggaat gcagtgtgat 840 attgatactg ctttagcatt tgaatcagaa gcatttggag aatgcttttc aacagaggat 900 caaaaggatg caatgacagc tttcatagag aaaagaaaaa ttgaaggctt caaaaataga 960 taggaggtaa gtttatatgg attttaattt aacaagagaa caagaattag taagacagat 1020 ggttagagaa tttgctgaaa atgaagttaa acctatagca gcagaaattg atgaaacaga 1080 aagatttcca atggaaaatg taaagaaaat gggtcagtat ggtatgatgg gaattccatt 1140 ttcaaaagag tatggtggcg caggtggaga tgtattatct tatataatcg ccgttgagga 1200 attatcaaag gtttgcggta ctacaggagt tattctttca gcacatacat cactttgtgc 1260 ttcattaata aatgaacatg gtacagaaga acaaaaacaa aaatatttag tacctttagc 1320 taaaggtgaa aaaataggtg cttatggatt gactgagcca aatgcaggaa cagattctgg 1380 agcacaacaa acagtagctg tacttgaagg agatcattat gtaattaatg gttcaaaaat 1440 attcataact aatggaggag ttgcagatac ttttgttata tttgcaatga ctgacagaac 1500 taaaggaaca aaaggtatat cagcatttat aatagaaaaa ggcttcaaag gtttctctat 1560 tggtaaagtt gaacaaaagc ttggaataag agcttcatca acaactgaac ttgtatttga 1620 agatatgata gtaccagtag aaaacatgat tggtaaagaa ggaaaaggct tccctatagc 1680 aatgaaaact cttgatggag gaagaattgg tatagcagct caagctttag gtatagctga 1740 aggtgctttc aacgaagcaa gagcttacat gaaggagaga aaacaatttg gaagaagcct 1800 tgacaaattc caaggtcttg catggatgat ggcagatatg gatgtagcta tagaatcagc 1860 tagatattta gtatataaag cagcatatct taaacaagca ggacttccat acacagttga 1920 tgctgcaaga gctaagcttc atgctgcaaa tgtagcaatg gatgtaacaa ctaaggcagt 1980 acaattattt ggtggatacg gatatacaaa agattatcca gttgaaagaa tgatgagaga 2040 tgctaagata actgaaatat atgaaggaac ttcagaagtt cagaaattag ttatttcagg 2100 aaaaattttt agataattta aggaggttaa gaggatgaat atagttgttt gtttaaaaca 2160 agttccagat acagcggaag ttagaataga tccagttaag ggaacactta taagagaagg 2220 agttccatca ataataaatc cagatgataa aaacgcactt gaggaagctt tagtattaaa 2280 agataattat ggtgcacatg taacagttat aagtatggga cctccacaag ctaaaaatgc 2340 tttagtagaa gctttggcta tgggtgctga tgaagctgta cttttaacag atagagcatt 2400 tggaggagca gatacacttg cgacttcaca tacaattgca gcaggaatta agaagctaaa 2460 atatgatata gtttttgctg gaaggcaggc tatagatgga gatacagctc aggttggacc 2520 agaaatagct gagcatcttg gaatacctca agtaacttat gttgagaaag ttgaagttga 2580 tggagatact ttaaagatta gaaaagcttg ggaagatgga tatgaagttg ttgaagttaa 2640 gacaccagtt cttttaacag caattaaaga attaaatgtt ccaagatata tgagtgtaga 2700 aaaaatattc ggagcatttg ataaagaagt aaaaatgtgg actgccgatg atatagatgt 2760 agataaggct aatttaggtc ttaaaggttc accaactaaa gttaagaagt catcaactaa 2820 agaagttaaa ggacagggag aagttattga taagcctgtt aaggaagcag ctgatatgtt 2880 gtctcaaaat taaaagaaga acacatattt aagttaggag ggatttttca atgaataaag 2940 cagattacaa gggcgtatgg gtgtttgctg aacaaagaga cggagaatta caaaaggtat 3000 cattggaatt attaggtaaa ggtaaggaaa tggctgagaa attaggcgtt gaattaacag 3060 ctgttttact tggacataat actgaaaaaa tgtcaaagga tttattatct catggagcag 3120 ataaggtttt agcagcagat aatgaacttt tagcacattt ttcaacagat ggatatgcta 3180 aagttatatg tgatttagtt aatgaaagaa agccagaaat attattcata ggagctactt 3240 tcataggaag agatttagga ccaagaatag cagcaagact ttctactggt ttaactgctg 3300 attgtacatc acttgacata gatgtagaaa atagagattt attggctaca agaccagcgt 3360 ttggtggaaa tttgatagct acaatagttt gttcagacca cagaccacaa atggctacag 3420 taagacctgg tgtgtttttt gaaaaattac ctgttaatga tgcaaatgtt tctgatgata 3480 aaatagaaaa agttgcaatt aaattaacag catcagacat aagaacaaaa gtttcaaaag 3540 ttgttaagct tgctaaagat attgcagata tcggagaagc taaggtatta gttgctggtg 3600 gtagaggagt tggaagcaaa gaaaactttg aaaaacttga agagttagca agtttacttg 3660 gtggaacaat agccgcttca agagcagcaa tagaaaaaga atgggttgat aaggaccttc 3720 aagtaggtca aactggtaaa actgtaagac caactcttta tattgcatgt ggtatatcag 3780 gagctatcca gcatttagca ggtatgcaag attcagatta cataattgct ataaataaag 3840 atgtagaagc cccaataatg aaggtagcag atttggctat agttggtgat gtaaataaag 3900 ttgtaccaga attaatagct caagttaaag ctgctaataa ttaagataaa taaaaagaat 3960 tatttaaagc ttattatgcc aaaatactta tatagtattt tggtgtaaat gcattgatag 4020 tttctttaaa tttagggagg tctgtttaat gcattgatag ttctttaaat ttagggaggt 4080 ctgtttaatg aaaaaggtat gtgttatagg tgcaggtact atgggttcag gaattgctca 4140 ggcatttgca gctaaaggat ttgaagtagt attaagagat attaaagatg aatttgttga 4200 tagaggatta gattttatca ataaaaatct ttctaaatta gttaaaaaag gaaagataga 4260 agaagctact aaagttgaaa tcttaactag aatttccgga acagttgacc ttaatatggc 4320 agctgattgc gatttagtta tagaagcagc tgttgaaaga atggatatta aaaagcagat 4380 ttttgctgac ttagacaata tatgcaagcc agaaacaatt cttgcatcaa atacatcatc 4440 actttcaata acagaagtgg catcagcaac taaaactaat gataaggtta taggtatgca 4500 tttctttaat ccagctcctg ttatgaagct tgtagaggta ataagaggaa tagctacatc 4560 acaagaaact tttgatgcag ttaaagagac atctatagca ataggaaaag atcctgtaga 4620 agtagcagaa gcaccaggat ttgttgtaaa tagaatatta ataccaatga ttaatgaagc 4680 agttggtata ttagcagaag gaatagcttc agtagaagac atagataaag ctatgaaact 4740 tggagctaat cacccaatgg gaccattaga attaggtgat tttataggtc ttgatatatg 4800 tcttgctata atggatgttt tatactcaga aactggagat tctaagtata gaccacatac 4860 attacttaag aagtatgtaa gagcaggatg gcttggaaga aaatcaggaa aaggtttcta 4920 cgattattca aaataagttt acaagaatcc ccattatcaa atggggattt tttatatata 4980 atataatttt aga 4993 // |
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