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EBI DbfetchID U15171; SV 1; linear; genomic DNA; STD; PRO; 4740 BP. XX AC U15171; XX DT 09-OCT-1994 (Rel. 41, Created) DT 17-APR-2005 (Rel. 83, Last updated, Version 5) XX DE Streptococcus pneumoniae type 3 capsular polysaccharide biosynthesis DE cassette, UDP-glucose dehydrogenase (cps3D), type 3 synthase (cps3S), and DE glucose-1-phosphate uridylyltransferase (cps3U) genes, complete cds. XX KW . XX OS Streptococcus pneumoniae OC Bacteria; Firmicutes; Lactobacillales; Streptococcaceae; Streptococcus. XX RN [1] RP 1-4740 RX DOI; 10.1084/jem.181.3.973 RX PUBMED; 7869055. RA Dillard J.P., Vandersea M.W., Yother J.; RT "Characterization of the cassette containing genes for type 3 capsular RT polysaccharide biosynthesis in Streptococcus pneumoniae"; RL J. Exp. Med. 181(3):973-983(1995). XX RN [2] RP 1-4740 RA Yother J.; RT ; RL Submitted (27-SEP-1994) to the EMBL/GenBank/DDBJ databases. RL Janet Yother, Microbiology, University of Alabama at Birmingham, 845 19th RL St. S., Birmingham, AL 35294, USA XX CC NCBI gi: 556001 XX FH Key Location/Qualifiers FH FT source 1..4740 FT /organism="Streptococcus pneumoniae" FT /strain="Type 3 WU2" FT /mol_type="genomic DNA" FT /db_xref="taxon:1313" FT misc_feature 1..934 FT /note="non-coding region containing sequences repeated in FT the S. pneumoniae chromosome" FT -35_signal 935..940 FT -10_signal 957..962 FT RBS 1013..1016 FT /gene="cps3D" FT CDS 1027..2211 FT /codon_start=1 FT /transl_table=11 FT /gene="cps3D" FT /product="UDP-glucose dehydrogenase" FT /note="Cps3D; NCBI gi: 556002" FT /db_xref="GOA:Q57346" FT /db_xref="HSSP:1DLJ" FT /db_xref="InterPro:IPR001732" FT /db_xref="UniProtKB/Swiss-Prot:Q57346" FT /protein_id="AAC43311.1" FT /translation="MKIAIAGSGYVGLSLAVLLAQHHEVKVIDVIKDKVESINNRKSPI FT KDEAIEKYLVEKELNLEASLDPAHVYKDVEYAIIATPTNYDVDLNQFDTSSVEAAIKTC FT MEYNDTCTIVIKSTIPEGYTKEVREKFNTDRIIFSPEFLRESKALYDNLYPSRIVVGTD FT LDDSELTKRAWQFADLLKGGAIKEEVPILVVAFNEAEVAKLFSNTYLATRVAYFNEIDT FT YSEVKGLNPKTIIDIVCYDPRIGSDYNNPSFGYGGYCLPKDTKQLKASFRDVPENLITA FT VVQSNKTRKDYIAGAILAKQPSVVGIYRLIMKSDSDNFRSSAVKGVMERLDNYGKEIVI FT YEPTIECDTFMGYRVIKSLDEFKNISDIVVANRMNDDLRDIQEKLYTRDLFGRE" FT RBS 2211..2215 FT /gene="cps3S" FT CDS 2227..3477 FT /codon_start=1 FT /transl_table=11 FT /gene="cps3S" FT /product="type 3 synthase" FT /note="Cps3S; NCBI gi: 556003" FT /db_xref="InterPro:IPR001173" FT /db_xref="UniProtKB/TrEMBL:Q54611" FT /protein_id="AAC43312.1" FT /translation="MYTFILMLLDFFQNHDFHFFMLFFVFILIRWAVIYFHAVRYKSYS FT CSVSDEKLFSSVIIPVVDEPLNLFESVLNRISRHKPSEIIVVINGPKNERLVKLCHDFN FT EKLENNMTPIQCYYTPVPGKRNAIRVGLEHVDSQSDITVLVDSDTVWTPRTLSELLKPF FT VCDKKIGGVTTRQKILDPERNLVTMFANLLEEIRAEGTMKAMSVTGKVGCLPGRTIAFR FT NIVERVYTKFIEETFMGFHKEVSDDRSLTNLTLKKGYKTVMQDTSVVYTDAPTSWKKFI FT RQQLRWAEGSQYNNLKMTPWMIRNAPLMFFIYFTDMILPMLLISFGVNIFLLKILNITT FT IVYTASWWEIILYVLLGMIFSFGGRNFKAMSRMKWYYVFLIPVFIIVLSIIMCPIRLLG FT LMRCSDDLGWGTRNLTE" FT -35_signal 3640..3645 FT -10_signal 3664..3669 FT terminator 3718..3738 FT /note="possible attenuator of cps3U expression" FT RBS 3765..3768 FT /gene="cps3U" FT CDS 3775..4695 FT /codon_start=1 FT /transl_table=11 FT /gene="cps3U" FT /product="glucose-1-phosphate uridylyltransferase" FT /note="Cps3U; NCBI gi: 556004" FT /db_xref="GOA:Q54800" FT /db_xref="InterPro:IPR005835" FT /db_xref="UniProtKB/Swiss-Prot:Q54800" FT /protein_id="AAC43313.1" FT /translation="MKKVKKAVIPAAGLGTRFLPATKALAKEMLPIVDRPTIHFVIEEA FT LRSGIEDILVVTGKSKRSIEDYFDSTFELEYSLRKQGKMELLKSVNESTDIKVHFVRQS FT SPRGLGDAVLQAKSFVGDDPFVVMLGDDLMDITDSTAVPLTRQLMDDYNATQASTIAVM FT PVRYEDVSSYGVISPRLESSNGLYSVDAFVEKPKPEEAPSHLAIIGRYLLTPEIFSILE FT TQKPGAGNEIQLTDAIDTLNKTQSVFAREFVGKRYDVGDKFNFMKTSIDYALQHPQIKE FT SLKNYVIALGKQLEKLDDCSSSGHL" XX SQ Sequence 4740 BP; 1538 A; 639 C; 1008 G; 1555 T; 0 other; gagctccaat caaagggtgt ttgtacactt tttgacagag ggtaatcgct agaggacagc 60 aaacggccat agtagtgaaa aatccagcac ctaaagcaga caaaagggtt gccatcaggt 120 ataaaatcat gtagagggcg ttagggtagg tgcgtgtgcg gtagagaatg tgttgagcca 180 aaacatcaag agtaccgtta gttattgcaa agttataaaa gagagagacg ctaaaaatgg 240 taaaaaagag tgaggttggc caaaatgaag aagttctttg gggcttaatc ccatgagagt 300 ggttgcgatg aggtaagaaa aagcaatagc cagcaggtca atattgattt tggtgcggta 360 accaattcca atggctagag caatggcgct aatcattatt aaatgaatca ttgaattgtc 420 ctttagttag aatatagaaa gaggatagat tgaagttcga gaatactggg tgtcttctga 480 tgttaagtgg ttgtgtcaaa accatcccaa atggcataaa ttgcgtggaa ttggaatgac 540 tcgtaatact attgatagag atggtattac aagtcaagac gttcgctatt ttatctttaa 600 ctttaagctt agtgtagtag ccttttgcca tagtgttcga ggtcattggt cagcagaaag 660 tatgcattgg ttattggatg tggtttatcg tgaagaccat catcagaccc tggataaacg 720 agctgctttt aaccttaatc ttatcgaaaa atgtgtttat attttctaaa agtgatggta 780 ttttctaaaa aagacctcag ctatcgatgc aaacaacggt atatctctgt acatttggaa 840 gattatttgg aaacagaggt taggaaagta atcagttaac gggatatctt ttcaaagctg 900 atactaaggc acaaaaaaaa gtttgatatt ccccttgaca atagataaaa ttattatata 960 attaaactat tgctttttaa ataaagtgag aatattaata atgcagagaa agaggactgt 1020 agtaaaatga aaattgccat tgcaggaagt ggttatgtag gtctgtcttt agcggtgcta 1080 ctagctcagc atcatgaagt taaggtcatt gatgttataa aggataaggt agagtcgata 1140 aacaatagaa aatctccaat taaggatgag gcgattgaga aatacttagt tgaaaaagag 1200 ttgaatcttg aagcctcctt agatcctgca cacgtttata aagacgtgga gtatgctatt 1260 attgctactc cgactaatta tgatgtagac ttaaatcagt ttgatacatc ttcagttgaa 1320 gctgctatca agacttgtat ggaatataat gatacttgta caatcgtaat caaaagtact 1380 attcctgaag ggtatactaa agaagtgagg gaaaagttta atacagatcg tattattttt 1440 tctccagagt ttctacgtga atccaaagct ttatatgata atttgtatcc atctagaatt 1500 gttgtaggaa ctgatttgga tgattctgag ttaacaaaaa gagcatggca gtttgcagat 1560 ctacttaaag gtggagctat taaggaagag gttccgatac tggttgttgc ttttaatgaa 1620 gcagaggttg caaaattgtt tagtaacact tacttggcaa ctcgcgtagc ttattttaat 1680 gagatagata catatagcga ggtaaaaggg cttaatccca agacaattat tgatattgtt 1740 tgttatgatc ctagaattgg atcagactat aataacccta gctttggtta cggagggtat 1800 tgcttaccaa aagacacaaa gcaattgaaa gcaagtttta gggatgttcc tgaaaatctg 1860 attacagctg tggtgcaatc taataaaaca agaaaagatt atatagctgg agctattcta 1920 gctaaacaac ctagtgttgt aggtatttat agattaatta tgaaatctga ttctgataat 1980 tttcgttcta gtgctgttaa gggagttatg gaacgtttgg acaattatgg taaagaaatt 2040 gttatttacg aacctactat tgagtgtgat acttttatgg gatacagagt aattaaatct 2100 ttagatgaat ttaagaatat ttctgacatt gttgtagcga atcgtatgaa cgatgattta 2160 agggatatac aagaaaaact ctatacacgc gatttatttg gcagagaata aggggaaata 2220 atttttatgt atacatttat tttaatgttg ttggattttt ttcagaatca tgattttcat 2280 ttctttatgt tgttttttgt ctttattctt attcgttggg cggttatata ttttcatgct 2340 gtcagatata agtcctacag ttgtagtgta agtgatgaga agttatttag ttctgtaatt 2400 atccctgtcg tggatgaacc acttaatctt tttgaaagtg tactgaatag aatttccaga 2460 cataaaccat ccgaaattat tgtggttatt aacggcccaa aaaacgagag acttgtaaaa 2520 ctttgtcatg attttaatga aaaattagaa aataatatga ctccaattca atgttattac 2580 actcctgttc ctggcaagag aaatgctatc cgcgttgggc tggagcatgt ggattcgcag 2640 agtgatatta cagttctagt agatagtgat acagtatgga cgcctagaac cttgagtgag 2700 ttgctgaagc cttttgtttg cgataaaaaa ataggtgggg taacgacaag acaaaaaatt 2760 cttgaccctg agcgtaatct cgtgacaatg tttgctaact tgttagagga aattagggca 2820 gaaggaacta tgaaagcaat gagtgtgact ggtaaagtag ggtgcttacc tggtcgaaca 2880 attgctttta gaaatatagt ggagagagtg tatacaaagt ttatagaaga gactttcatg 2940 ggatttcata aggaagtttc tgatgataga agtcttacaa atttgacttt aaaaaaaggc 3000 tataaaactg ttatgcagga tacttctgtt gtgtatacag atgctcctac aagttggaaa 3060 aagttcatta gacagcaact aaggtgggca gaaggttctc agtataacaa tctaaagatg 3120 actccttgga tgattagaaa tgcccctctt atgtttttta tttattttac agatatgatt 3180 ttacctatgc tacttattag ctttggtgtg aatatattcc tgttgaaaat attaaatata 3240 actacaattg tttatacagc ttcatggtgg gaaattattt tatatgttct tttgggaatg 3300 atttttagct ttggaggaag aaactttaaa gctatgtcta gaatgaagtg gtattatgta 3360 tttcttattc ctgtttttat aatcgttttg agtataatta tgtgccctat taggctatta 3420 ggacttatga gatgttctga tgatttaggg tggggaacta ggaatttaac agagtgagat 3480 aaatagtagt gcgtatatag agtatttact cagagtatta attgattttt gaaaaggaaa 3540 agtgtttttt aatgttaaga aagaacttga aatatcagat tatgacacga gctggaacaa 3600 ttttagctat cttgtttttc attatattag ggattattgt tgaagttttg ttttaaggct 3660 cattgtcatc tgtagtggct cacttcagac gtaagggtca tattttaatg tgaaaagagt 3720 gttaaaagat taatcactta tattatttta atagaaatag tgtaaggaat tgttatgaaa 3780 aaagtaaaaa aagctgttat tcctgctgca gggctgggca cacgattttt gcctgccact 3840 aaagctttgg caaaagaaat gcttccaatt gtagaccgcc ccacaattca ttttgtcatt 3900 gaagaagctt tacgttcggg tattgaagat attctagtag ttactggaaa gagtaaacgt 3960 tctattgaag attattttga ttcaactttt gaattggaat atagtcttag aaaacaagga 4020 aagatggaac ttcttaagtc agttaacgaa tcgactgata ttaaagtaca tttcgttcgt 4080 caaagttcac cacgtggtct tggtgacgct gttctccaag cgaagtcttt tgttggtgac 4140 gatccctttg ttgtaatgct tggtgatgac cttatggata tcaccgactc aactgctgta 4200 cctttaacaa gacaattgat ggatgattac aacgcaacac aggcttcaac tatcgcagta 4260 atgcctgtta gatatgaaga tgtttcttct tatggtgtga tttctcctag attggaaagt 4320 agtaatggcc tctatagtgt tgatgctttt gtagagaaac caaaaccaga agaagcgcct 4380 agccatttag ctattattgg acgttatcta cttactcctg agattttttc tatattagaa 4440 acccaaaagc caggagcagg taatgaaatt caattgacag atgctattga tacattgaat 4500 aagacacaga gtgtttttgc gcgtgaattt gtgggcaaac gttacgatgt tggtgataag 4560 tttaatttta tgaaaacatc aattgattat gctcttcaac atcctcagat taaagagagt 4620 ttaaaaaatt acgttattgc acttggtaag caattggaga agctagatga ctgttcgtca 4680 agtggacacc tatgaattgt atagaaagtt atcaaaaatg gctaaatgtc cctgatcttc 4740 // |
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