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EBI DbfetchID U12340; SV 1; linear; genomic DNA; STD; PRO; 5211 BP. XX AC U12340; XX DT 22-AUG-1994 (Rel. 40, Created) DT 17-APR-2005 (Rel. 83, Last updated, Version 6) XX DE Bacillus stearothermophilus XL-65-6 phosphoenolpyruvate-dependent DE phosphotransferase system glucose-specific permease (ptsG') gene, partial DE cds, HPr (ptsH), enzyme I (ptsI), and PtsT (ptsT) genes, complete cds, and DE wall associated protein precursor (wapA') gene, complete cds. XX KW . XX OS Geobacillus stearothermophilus OC Bacteria; Firmicutes; Bacillales; Bacillaceae; Geobacillus. XX RN [1] RP 1-5211 RX PUBMED; 7670643. RA Lai X., Ingram L.O.; RT "Discovery of a ptsHI operon, which includes a third gene (ptsT), in the RT thermophile Bacillus stearothermophilus"; RL Microbiology (Reading, Engl.) 141:1443-1449(1995). XX RN [2] RP 1-5211 RA Lai X.; RT ; RL Submitted (18-JUL-1994) to the EMBL/GenBank/DDBJ databases. RL Xiaokuang Lai, Microbiology and Cell Science, University of Florida, RL Gainesville, Gainesville, FL 32611, USA XX FH Key Location/Qualifiers FH FT source 1..5211 FT /organism="Geobacillus stearothermophilus" FT /strain="XL-65-6" FT /mol_type="genomic DNA" FT /note="isolated from rotting wood" FT /db_xref="taxon:1422" FT CDS <1..976 FT /codon_start=2 FT /transl_table=11 FT /gene="ptsG'" FT /product="PTS glucose-specific permease" FT /note="thermophilic, cytoplasmic protein" FT /db_xref="GOA:P42015" FT /db_xref="HSSP:1GPR" FT /db_xref="InterPro:IPR011535" FT /db_xref="UniProtKB/Swiss-Prot:P42015" FT /protein_id="AAA86047.1" FT /translation="HLLNVKIGMTFSGGVIDFLLFGVLPNRTAWWLVIPVGLVFAVIYY FT FGFRFAIRKWDLATPGREKTVEEAPKAEAAAAGDLPYEVLAALGGKENIEHLDACITRL FT RVSVHDIGRVDKDRLKALGAAGVLEVGNNVQAIFGPKSDMLKGQIQDIMQGKAPARAEE FT KPKTAASEAAESETIASPMSGEIVPLAEVPDQVFSQKMMGDGFAVMPTDGTVVSPVDGK FT IINVFPTKHAIGIQSAGGHEILIHVGIDTVKLNGQGFEALVKEGDEVKKGQPILRVDLD FT YVKQNAPSIVTPVIFTNLQAGETVHVNKQGPVARGEDAVVTIR" FT promoter 1015..1046 FT /gene="ptsH" FT /note="putative" FT RBS 1066..1072 FT /gene="ptsH" FT /note="putative" FT CDS 1083..1349 FT /codon_start=1 FT /transl_table=11 FT /gene="ptsH" FT /product="PTS HPr" FT /note="thermophilic, cytoplasmic protein" FT /db_xref="GOA:P42013" FT /db_xref="InterPro:IPR002114" FT /db_xref="PDB:1Y4Y" FT /db_xref="UniProtKB/Swiss-Prot:P42013" FT /experiment="experimental evidence, no additional details FT recorded" FT /protein_id="AAA86048.1" FT /translation="MAEKTFKVVSDSGIHARPATILVQTASKFNSEIQLEYNGKTVNLK FT SIMGVMSLGIPKGATIKITAEGADAAEAMAALTDTLAKEGLAE" FT RBS 1329..1335 FT /gene="ptsH" FT /note="putative" FT CDS 1349..3085 FT /codon_start=1 FT /transl_table=11 FT /gene="ptsI" FT /product="PTS enzyme I" FT /note="thermophilic, cytoplasmic protein" FT /db_xref="GOA:P42014" FT /db_xref="HSSP:1EZC" FT /db_xref="InterPro:IPR015813" FT /db_xref="UniProtKB/Swiss-Prot:P42014" FT /experiment="experimental evidence, no additional details FT recorded" FT /protein_id="AAA86049.1" FT /translation="MGNAIREKTIHGIAASSGIAIAKAYRLETPDLAAEKRTVADVEAE FT IARLEAAVAKAKEELEAIKQHALEKLGEDKAAIFAAHLLVLDDPELLNPIKEKIQTERV FT NAEYALDETALFFISMFEAMDNEYMKERAADIRDVTKRVLAHLLGVTISNPSLISEEVV FT IIAEDLTPSDTAQLNRQYVKGFATDIGGRTSHSAIMARSLEIPAVVGTKTVTAEVKNGD FT IVIVDGLDGQVIINPSPELLAQYEQKRARYEAQKAEWAKLVHEATVTADGIHVELAANI FT GTPDDVKGALANGAEGIGLYRTEFLYMGRSELPTEDEQFVAYKTVLEQMNGKPVVVRTL FT DIGGDKELPYLQLPKEMNPFLGFRAIRLCLEMQDMFRTQLRALLRASVYGNLKIMFPMI FT ATLDEFRQAKAILLEEKEALLRQGVAVADGIEVGMMVEIPAAAVMADQFAKEVDFFSIG FT TNDLIQYTMAADRMNERVAYLYQPYNPAILRLISHVIDAAHREGKWVGMCGEMAGDPIA FT IPILLALGLDEFSMSATSILPARAQLKKLAKEEAARIKETVLSLGTAEEVVSFVKRTFS FT LA" FT CDS 3086..3553 FT /codon_start=1 FT /transl_table=11 FT /gene="ptsT" FT /product="PtsT" FT /note="thermophilic, cytoplasmic protein" FT /db_xref="UniProtKB/Swiss-Prot:P42019" FT /experiment="experimental evidence, no additional details FT recorded" FT /protein_id="AAA86050.1" FT /translation="MKAWRRLRSPRFFRFALCQQRRRRFIAIPLPDRKRKADSDNFRHL FT VNNVQIRIRKHAHISRVLHHDSHDADVSDRWQRFAERRARGKGARADGDRMDKRLRPDN FT AVDCHYICSVFVLQLFGNASDVCRIDGLPERADDGQMIRISVFQQRADERP" FT CDS 4383..5147 FT /codon_start=1 FT /transl_table=11 FT /gene="wapA'" FT /product="precursor for wall associated protein" FT /db_xref="GOA:P42018" FT /db_xref="UniProtKB/Swiss-Prot:P42018" FT /protein_id="AAA86051.1" FT /translation="MVVWALVMPSVPTKGLAETIRSGIDRVEPKEEPPKVPQAPKRDLK FT PGEIIEERTENTKVYYNGDGTFTKKIYFEPIHVKKKGQKIFEEVSSSLTDSTNNTNYVE FT TENTILETNFYKKMVDGEYANFHYNGYSISYSILEAAGNDVQSIKAKDVAAVYKKKDNK FT ILHKNIFPSIDLQNITFNESTKEDLVLHSVGYHIFKFRLKTDLQADIQDDGSILLTNQE FT HEKVFELPKPFMVDSNVDEHSGEVQRLRECNV" XX SQ Sequence 5211 BP; 1378 A; 1227 C; 1443 G; 1163 T; 0 other; gcacttgttg aacgtgaaaa tcgggatgac cttctccggc ggggtcattg acttcttgct 60 gtttggcgtt ctgccaaacc ggacggcatg gtggctcgtc attccggtcg gcttggtgtt 120 tgccgtgatt tattatttcg ggttccgctt tgcgatccgc aaatgggatt tggcgacgcc 180 gggccgggaa aaaacggtcg aggaagcacc gaaagccgag gcggccgctg ctggcgatct 240 gccgtacgaa gtgctcgccg cccttggcgg aaaggaaaac atcgaacatt tggacgcatg 300 cattacgcgt ttgcgcgttt cggtccatga tatcggccgg gtcgataaag accggttgaa 360 agcgctcggc gccgcagggg tgcttgaagt cggcaacaac gtgcaagcga ttttcggtcc 420 gaaatcggat atgttaaaag ggcaaattca agacatcatg caaggaaaag cgccggcccg 480 ggcggaagag aaaccgaaaa cagcggcctc ggaagccgcg gagtccgaaa cgatcgcttc 540 gccgatgtcc ggagaaatcg ttccgctcgc tgaagtgccg gaccaagtgt tttcgcaaaa 600 aatgatgggc gatgggttcg cagtcatgcc gacggacggc acggtcgtct ctccggttga 660 tgggaagatc atcaacgtct ttccgacgaa gcacgccatt ggcattcagt cggccggcgg 720 gcatgaaatt ttgatccacg tcggcatcga tacggtgaag ctgaacggcc aagggtttga 780 agcgcttgtg aaggaaggcg acgaagtgaa aaaaggacag ccgattttgc gcgtcgacct 840 tgattatgtc aaacagaacg ccccatcgat cgtcacaccg gtgattttca cgaatctcca 900 agccggcgaa acggttcacg tcaacaagca aggcccggtt gctcgaggag aagacgctgt 960 tgtgacgatc cgctaaactg cttattcgcg gcggggcggg accaagcatt ttccgttgcc 1020 tccccgcccc tgcatttgat atgatggcaa agaaaaaatt gaagaaaagg ggaatgatga 1080 acatggcaga aaaaacgttt aaagtcgttt ctgattccgg catccacgct cgtccggcga 1140 cgattttggt gcaaacggcg agcaaattca acagcgaaat ccagcttgag tacaacggca 1200 aaacggtgaa cttgaaatcg atcatgggcg ttatgtcgtt aggaattccg aaaggggcga 1260 cgatcaaaat cacggcagaa ggggccgatg cggcagaagc aatggcggcg ttaacggata 1320 cgttggcgaa agagggtctt gcagaataat gggaaacgca atccgcgaaa aaacgatcca 1380 tgggattgct gcatcgagcg gtattgccat cgcgaaggca taccgcttag aaacccctga 1440 tttggcagcc gaaaaacgga cggtcgccga cgtcgaggcg gaaattgcgc ggcttgaggc 1500 ggcggtcgcg aaagcgaaag aagagctgga agccatcaaa cagcatgcct tggaaaagct 1560 tggcgaagac aaagctgcca tttttgccgc ccacttgctt gtgcttgacg acccagaatt 1620 gctgaacccg attaaagaaa aaatccaaac ggagcgcgtc aatgccgagt acgctctcga 1680 tgaaaccgcc ttgtttttca tctcgatgtt cgaggcgatg gacaatgaat atatgaaaga 1740 acgggccgct gacatccgcg atgtgacaaa gcgcgtcctc gcccatctgc ttggcgtcac 1800 aatctcgaac ccgagcctca tttctgagga agtcgtgatc atcgcggaag acttgacgcc 1860 atccgatacg gcgcagctga accgccaata tgtgaaagga tttgccaccg acatcggcgg 1920 gcggacgtcg cattcagcaa ttatggcccg ttcgctcgaa atccctgccg tcgtcggcac 1980 gaagacggtg acggcggaag taaaaaacgg cgacatcgtc attgtcgatg ggctcgacgg 2040 ccaagtaatc atcaatccat cgccggagtt gctcgcccaa tatgaacaaa aacgggcgcg 2100 ctacgaggcg caaaaggcag aatgggcgaa actcgttcac gaggcgaccg tgacggctga 2160 cggcattcat gtcgagctgg ctgccaacat cggcacgcca gacgatgtga aaggagcgtt 2220 ggcgaacgga gcggaaggaa tcggattgta tcgcacagaa tttctataca tgggacgctc 2280 ggaactgccg acggaagacg aacagtttgt ggcttacaaa acggtgctgg aacaaatgaa 2340 tggcaagccg gtcgttgtgc ggacgcttga cattggcggc gacaaagagc tgccgtattt 2400 gcagctgccg aaagaaatga atccgttctt agggttccgg gcaatccgcc tttgccttga 2460 gatgcaagat atgttccgca cgcagctgcg cgccttgttg cgggcgagtg tgtacggcaa 2520 tttgaaaatc atgttcccga tgattgcgac gctcgatgaa ttccgccaag cgaaagccat 2580 tttgcttgaa gaaaaagagg cgctcctccg ccaaggggtt gccgttgcgg atggaattga 2640 agtcggcatg atggtggaaa ttccggctgc cgccgtcatg gcggaccagt ttgcgaaaga 2700 agtcgatttc ttcagcattg gaacgaacga cctgatccaa tatacgatgg cggccgatcg 2760 gatgaatgag cgggttgcgt atttgtacca gccgtacaac ccggctattt tgcggctcat 2820 cagccacgtg atcgatgccg cccatcgcga aggaaaatgg gtcgggatgt gcggggaaat 2880 ggccggcgac ccgatcgcga ttccgatttt gcttgcttta ggccttgatg agttcagcat 2940 gagcgccact tcgattttgc cggcgcgcgc ccagttaaaa aagctggcga aagaagaggc 3000 ggcccgcatc aaagagacgg tgctgtcgct cggcacggct gaggaagtcg tgtcgtttgt 3060 caaacgaaca ttttcacttg cctgaatgaa agcgtggcga cgtttacgtt cgccgcgctt 3120 ttttcgtttt gcgttatgcc aacagcggcg tcgacgattc atagccatac cgctgccgga 3180 ccgtaagcga aaggccgata gcgataactt tcgccatttg gtaaacaatg tgcaaattcg 3240 tatacggaag cacgctcata tcagccgtgt cctccatcat gactcccatg acgctgatgt 3300 ctccgaccgg tggcagcgat ttgccgagcg ccgtgccagg ggaaagggcg ccagggcgga 3360 tggcgatcgt atggacaaac ggctgcggcc cgacaatgct gtcgattgcc actacatatg 3420 ctccgtgttc gttttgcaat tgttcggaaa cgcgtcggat gtttgtcgca tcgacgggtt 3480 gcctgagcgt gccgatgacg gtcaaatgat ccggataagc gttttccagc agcgtgccga 3540 cgaacggccc taagctgtcg ccgttgatgc gattgctgcc gatgcaaacg acggtcagat 3600 gacggatgtg cgccggcagc aaggaaaaca gttcattgcg aatgagcaaa ggagcgagcg 3660 gatcgctgta ggaaatcgtc ctcatcaatc gcgattcctt tctgtctata gtctcctttt 3720 cttattcgcg gccgctgccg gcgattcctt tcttttcagg gttgcaaatt tttccttatg 3780 tttgcttaaa cgggttgcgc cattccatta acacggcatg gttgagcgat tcttcgtctt 3840 ctaaactttc ttgctgcacg cggatgagat cagcaaagtc gtctgtcgta tagttgcgga 3900 tgaccgcttc gcgcggggtg ttgccgtcaa agacgtataa acgtttatcg tacatgctga 3960 tccccctttc gttcgtttta ccctcatcat aaccgaaacc tagaaaaagc gccaagaaag 4020 gtccatggac aagtttgccc tcgacccggg gatgaaaatc accgcttgcc gacgggattt 4080 tcacggaaag cgggtagctt ggtgttcaaa taaaacgatc atttacagtc ttacgctaag 4140 tgatcaagat caccatcgca agcaaccact ggcaatcttc gtttaggact ctttctccat 4200 ttcacataca aataggttta ctcgcaaaag cttccgaaga aaatgaaaaa ccatgtcgaa 4260 ctttgtctgt cgaaaatgat ggatggcagg gagcaggaaa gatacaattt gattgtaaaa 4320 gtaatggaaa tcgggaagga gagcatatga gaaacattcg tttgtgatga tggttggttt 4380 ggatggtcgt atgggcttta gtgatgccaa gcgtcccgac aaagggactt gccgaaacga 4440 tccgatcagg gattgaccgt gtagagccaa aagaggagcc gccaaaggtg ccgcaagccc 4500 cgaaacgtga tctcaagcca ggggaaatca tagaagaaag aactgagaat acaaaggttt 4560 actacaacgg cgatggcacg tttaccaaga agatttactt tgagcctatt catgtgaaga 4620 aaaagggaca aaaaatattt gaggaagtat cttctagcct aacggatagc accaataata 4680 cgaattacgt tgaaacagag aataccattt tggagacaaa cttttataaa aagatggtcg 4740 atggagaata cgccaatttc cattacaatg gatattcgat ttcctattct attttagagg 4800 cggcaggtaa tgacgtacaa tcgataaaag cgaaggatgt tgccgccgtc tataagaaaa 4860 aagacaacaa aattcttcac aaaaacattt ttccgagcat agacctacaa aatattacat 4920 ttaacgagtc aacaaaagaa gatttggttc ttcattcggt tggctatcat attttcaaat 4980 tccgcctgaa aacggatttg caagcagaca ttcaagacga tggctccatt ttattgacaa 5040 atcaagaaca tgaaaaggtg tttgaactgc cgaagccgtt tatggtggat tcgaatgtcg 5100 atgaacactc tggtgaagtg cagcgtctca gagaatgtaa cgtatgaatt acaacaggat 5160 atcaaggata tattttaacg gtcaaagccg accctgattg gctgaaggat c 5211 // |
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