EBI Dbfetch

ID   U12275; SV 1; linear; genomic DNA; STD; INV; 1886 BP.
XX
AC   U12275;
XX
DT   09-OCT-1994 (Rel. 41, Created)
DT   14-NOV-2006 (Rel. 89, Last updated, Version 6)
XX
DE   Plasmodium berghei ANKA dihydrofolate reductase thymidylate synthase
DE   (DHFR/TS) gene, complete cds.
XX
KW   .
XX
OS   Plasmodium berghei
OC   Eukaryota; Alveolata; Apicomplexa; Aconoidasida; Haemosporida; Plasmodium;
OC   Plasmodium (Vinckeia).
XX
RN   [1]
RP   1-1886
RX   DOI; 10.1016/0166-6851(94)00163-4
RX   PUBMED; 7891743.
RA   van Dijk M.R., McConkey G.A., Vinkenoog R., Waters A.P., Janse C.J.;
RT   "Mechanisms of pyrimethamine resistance in two different strains of
RT   Plasmodium berghei";
RL   Mol. Biochem. Parasitol. 68(1):167-171(1994).
XX
RN   [2]
RP   1-1886
RA   van Dijk M.R.;
RT   ;
RL   Submitted (14-JUL-1994) to the EMBL/GenBank/DDBJ databases.
RL   Melissa R. van Dijk, Parasitology/Malaria University of Leiden,
RL   Wassenaarseweg 62, Leiden, Zuid-Holland, 2300 Rc, The Netherlands
XX
FH   Key             Location/Qualifiers
FH
FT   source          1..1886
FT                   /organism="Plasmodium berghei"
FT                   /chromosome="7"
FT                   /host="Grammomys surdaster"
FT                   /strain="ANKA"
FT                   /isolate="8417"
FT                   /mol_type="genomic DNA"
FT                   /clone="pMD20"
FT                   /db_xref="taxon:5821"
FT   CDS             66..1829
FT                   /codon_start=1
FT                   /gene="DHFR/TS"
FT                   /product="dihydrofolate reductase thymidylate synthase"
FT                   /function="DNA synthesis"
FT                   /EC_number="1.5.1.3"
FT                   /db_xref="GOA:Q27713"
FT                   /db_xref="InterPro:IPR000398"
FT                   /db_xref="InterPro:IPR001796"
FT                   /db_xref="InterPro:IPR012262"
FT                   /db_xref="InterPro:IPR017925"
FT                   /db_xref="UniProtKB/Swiss-Prot:Q27713"
FT                   /experiment="experimental evidence, no additional details
FT                   recorded"
FT                   /protein_id="AAB60237.1"
FT                   /translation="MEDLSETFDIYAICACCKVLNDDEKVRCFNNKTFKGIGNAGVLPW
FT                   KCNLIDMKYFSSVTSYINENNYIRLKWKRDKYMEKHNLKNNVELNTNIISSTNNLQNIV
FT                   VMGKKSWESIPKKFKPLQNRINIILSRTLKKEDIVNENNNENNNVIIIKSVDDLFPILK
FT                   CTKYYKCFIIGGSSVYKEFLDRNLIKKIYFTRINNSYNCDVLFPEINENLFKITSISDV
FT                   YYSNNTTLDFIIYSKTKEINPNEEVPNNTFLGVCDEQNKAFDDEDDYTYFSFNKNKENI
FT                   KKNSEHAHNFKIYNSIKYKNHPEYQYLNIIYDIIMHGNKQDDRTGVGVLSKFGYMMKFN
FT                   LNEYFPLLTTKKLFIRGIIEELLWFIRGETNGNTLLEKNVRIWEANGTREFLDNRKLFH
FT                   REVNDLGPIYGFQWRHFGAEYTDMHDNYKDKGVDQLKNIINLIKNDPTCRRIILCAWNV
FT                   KNLDQMALPPCHILCQFYVFDGKLSCIMYQRSCDLGLGVPFNIASYSIFTYMIAQVCNL
FT                   QAAEFIHVLGNAHVYNNHIESLKIQLNRTPYPFPTLKLNPDIKNIEDFTISDFTVQNYV
FT                   HHDKINMDMAA"
FT   variation       394
FT                   /phenotype="R163; pyrimethamine resistance"
FT                   /gene="DHFR/TS"
FT                   /replace="a"
FT                   /note="causes Ser to Asp change"
FT   variation       595
FT                   /phenotype="pyrimethamine resistance line"
FT                   /gene="DHFR/TS"
FT                   /replace="t"
FT                   /note="causes Ser to Phe change"
XX
SQ   Sequence 1886 BP; 775 A; 175 C; 270 G; 666 T; 0 other;
     ataatagtat ttttcatttt gtatattgct tatatatata taaatacaca cctaaatgtt        60
     acaaaatgga agacttatct gaaacattcg atatatatgc aatatgtgca tgttgtaaag       120
     ttctgaacga tgatgaaaag gttagatgct ttaataataa aacgtttaag ggaattggaa       180
     atgcgggggt gttaccttgg aaatgtaatt taatcgatat gaaatatttt agttctgtaa       240
     catcatatat aaatgaaaat aattatataa gattgaaatg gaaaagagat aaatatatgg       300
     aaaaacataa tttaaaaaat aatgtagaac taaatactaa tataatttct tcaactaata       360
     atttacaaaa tattgtagta atgggaaaaa aaagttggga aagtattccc aaaaaattta       420
     aacctttaca aaatcgaata aacattattt tgtctagaac tttgaaaaaa gaagatattg       480
     taaacgaaaa taataatgaa aataataatg ttattataat taaaagtgta gatgatttat       540
     ttcctatttt aaaatgcaca aaatattaca aatgttttat tatagggggt tcgtctgttt       600
     ataaagaatt tttagatcgt aatttaataa aaaaaatata ttttacaaga ataaataatt       660
     cttataattg tgatgtttta ttcccagaaa taaacgaaaa tttgtttaaa ataacttcaa       720
     taagtgatgt atattatagt aacaacacaa ctttagattt tataatttat agtaagacaa       780
     aagaaataaa tccaaatgag gaagtaccta ataatacatt tttaggtgta tgtgatgaac       840
     aaaataaagc ctttgatgat gaagacgatt atacatattt cagtttcaat aaaaataaag       900
     aaaatattaa aaaaaattct gaacatgctc ataattttaa aatatataat agcataaaat       960
     ataaaaatca tcctgaatat caatatttaa atattatata tgatataata atgcatggaa      1020
     ataaacaaga tgatagaaca ggtgttggtg tgttaagtaa atttggatat atgatgaaat      1080
     ttaatttaaa tgaatatttt ccattattaa caacaaaaaa attatttata agaggtatta      1140
     tcgaagaatt attgtggttt ataagagggg aaacaaatgg aaatactttg ttagaaaaaa      1200
     acgtaagaat atgggaagct aatggaacaa gggaattttt agataataga aaattatttc      1260
     atagagaagt taatgatctc ggtccaattt atggatttca atggaggcat tttggtgctg      1320
     aatatacaga tatgcatgat aattataaag acaaaggagt tgatcaatta aaaaatatta      1380
     taaatttaat taaaaatgat cctacttgta gacgaattat tttgtgtgca tggaatgtaa      1440
     aaaatttaga tcaaatggca ttacctcctt gtcatatttt atgtcaattt tatgtttttg      1500
     acggaaaatt atcatgtatt atgtatcaaa gatcttgtga tttagggctt ggggttccat      1560
     tcaatattgc ttcctattct atatttacat atatgatagc acaagtatgt aacttacagg      1620
     cagctgaatt tatacatgta ttgggtaatg ctcatgttta taataatcat attgaaagct      1680
     taaagattca gttaaataga actccttacc cttttcctac tcttaaatta aatcctgaca      1740
     ttaaaaatat cgaggatttt acaatttctg attttactgt tcaaaattat gttcatcacg      1800
     ataaaataaa tatggatatg gcagcttaat gttcgttttt cttatttata tatttatacc      1860
     aattgattgt atttataact gtaaaa                                           1886
//