EBI Dbfetch

ID   U12012; SV 1; linear; mRNA; STD; PLN; 1969 BP.
XX
AC   U12012;
XX
DT   27-JUL-1994 (Rel. 40, Created)
DT   17-APR-2005 (Rel. 83, Last updated, Version 3)
XX
DE   Pinus taeda PT4CL1 4-coumarate-CoA ligase enzyme mRNA, complete cds.
XX
KW   .
XX
OS   Pinus taeda (loblolly pine)
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Coniferopsida; Coniferales; Pinaceae; Pinus; Pinus.
XX
RN   [1]
RP   1-1969
RX   DOI; 10.1104/pp.108.1.85
RX   PUBMED; 7784527.
RA   Voo K.S., Whetten R.W., O'Malley D.M., Sederoff R.R.;
RT   "4-coumarate:coenzyme a ligase from loblolly pine xylem. Isolation,
RT   characterization, and complementary DNA cloning";
RL   Plant Physiol. 108(1):85-97(1995).
XX
RN   [2]
RP   1-1969
RA   Voo K.S.;
RT   ;
RL   Submitted (07-JUL-1994) to the EMBL/GenBank/DDBJ databases.
RL   Kui S. Voo, Forestry Biotechnology Group, North Carolina State University,
RL   Rm 6113 Jordan Hall, Raleigh, NC 27695, USA
XX
FH   Key             Location/Qualifiers
FH
FT   source          1..1969
FT                   /organism="Pinus taeda"
FT                   /strain="L."
FT                   /mol_type="mRNA"
FT                   /dev_stage="mature wood"
FT                   /clone="PT4CL1"
FT                   /tissue_type="differentiating xylem"
FT                   /db_xref="taxon:3352"
FT   CDS             143..1756
FT                   /codon_start=1
FT                   /product="4-coumarate-CoA ligase enzyme"
FT                   /db_xref="GOA:P41636"
FT                   /db_xref="HSSP:1LCI"
FT                   /db_xref="InterPro:IPR000873"
FT                   /db_xref="UniProtKB/Swiss-Prot:P41636"
FT                   /protein_id="AAA92668.1"
FT                   /translation="MANGIKKVEHLYRSKLPDIEISDHLPLHSYCFERVAEFADRPCLI
FT                   DGATDRTYCFSEVELISRKVAAGLAKLGLQQGQVVMLLLPNCIEFAFVFMGASVRGAIV
FT                   TTANPFYKPGEIAKQAKAAGARIIVTLAAYVEKLADLQSHDVLVITIDDAPKEGCQHIS
FT                   VLTEADETQCPAVKIHPDDVVALPYSSGTTGLPKGVMLTHKGLVSSVAQQVDGENPNLY
FT                   FHSDDVILCVLPLFHIYSLNSVLLCALRAGAATLIMQKFNLTTCLELIQKYKVTVAPIV
FT                   PPIVLDITKSPIVSQYDVSSVRIIMSGAAPLGKELEDALRERFPKAIFGQGYGMTEAGP
FT                   VLAMNLAFAKNPFPVKSGSCGTVVRNAQIKILDTETGESLPHNQAGEICIRGPEIMKGY
FT                   INDPESTAATIDEEGWLHTGDVEYIDDDEEIFIVDRVKEIIKYKGFQVAPAELEALLVA
FT                   HPSIADAAVVPQKHEEAGEVPVAFVVKSSEISEQEIKEFVAKQVIFYKKIHRVYFVDAI
FT                   PKSPSGKILRKDLRSRLAAK"
FT   misc_binding    703..738
FT                   /bound_moiety="AMP"
FT   misc_feature    1296..1317
FT                   /note="encodes catalytic site"
XX
SQ   Sequence 1969 BP; 488 A; 500 C; 484 G; 497 T; 0 other;
     ctcattcaat tcttcccact gcaggctaca tttgtcagac acgttttccg ccatttttcg        60
     cctgtttctg cggagaattt gatcaggttc ggattgggat tgaatcaatt gaaaggtttt       120
     tattttcagt atttcgatcg ccatggccaa cggaatcaag aaggtcgagc atctgtacag       180
     atcgaagctt cccgatatcg agatctccga ccatctgcct cttcattcgt attgctttga       240
     gagagtagcg gaattcgcag acagaccctg tctgatcgat ggggcgacag acagaactta       300
     ttgcttttca gaggtggaac taatttctcg caaggtcgct gccggtctgg cgaagctcgg       360
     gttgcagcag gggcaggttg tcatgcttct ccttccgaat tgcatcgaat ttgcgtttgt       420
     gttcatgggg gcctctgtcc ggggcgccat tgtgaccacg gccaatcctt tctacaagcc       480
     gggcgagatc gccaaacagg ccaaggccgc aggcgcgcgc atcatagtta ccctggcagc       540
     ttatgttgag aaactggccg atctgcagag ccacgatgtg ctcgtcatca caatcgatga       600
     tgctcccaag gaaggttgcc aacatatttc cgttctgacc gaagccgacg aaacccaatg       660
     cccggccgtg aaaatccacc cggacgatgt cgtggcgttg ccctattctt ccggaaccac       720
     ggggctcccc aagggcgtga tgttaacgca caaaggcctg gtgtccagcg ttgcccagca       780
     ggtcgatggt gaaaatccca atctgtattt ccattccgat gacgtgatac tctgtgtctt       840
     gcctcttttc cacatctatt ctctcaattc ggttctcctc tgcgcgctca gagccggggc       900
     tgcgaccctg attatgcaga aattcaacct cacgacctgt ctggagctga ttcagaaata       960
     caaggttacc gttgccccaa ttgtgcctcc aattgtcctg gacatcacaa agagccccat      1020
     cgtttcccag tacgatgtct cgtccgtccg gataatcatg tccggcgctg cgcctctcgg      1080
     gaaggaactc gaagatgccc tcagagagcg ttttcccaag gccattttcg ggcagggcta      1140
     cggcatgaca gaagcaggcc cggtgctggc aatgaaccta gccttcgcaa agaatccttt      1200
     ccccgtcaaa tctggctcct gcggaacagt cgtccggaac gctcaaataa agatcctcga      1260
     tacagaaact ggcgaatctc tcccgcacaa tcaagccggc gaaatctgca tccgcggacc      1320
     cgaaataatg aaaggatata ttaacgaccc ggaatccacg gccgctacaa tcgatgaaga      1380
     aggctggctc cacacaggcg acgtcgaata cattgacgat gacgaagaaa tcttcatagt      1440
     cgacagagta aaggagatta tcaaatataa gggcttccag gtggctcctg ctgagctgga      1500
     agctttactt gtggctcatc cgtcaatcgc tgacgcagca gtcgttcctc aaaagcacga      1560
     ggaggcgggc gaggttccgg tggccttcgt ggtgaagtcg tcggaaatca gcgagcagga      1620
     aatcaaggag ttcgtggcaa agcaggtgat tttctacaag aaaatacaca gagtttactt      1680
     tgtggatgcg attcctaagt cgccgtccgg caagattctg agaaaggatt tgagaagcag      1740
     actggcagca aaatgaaaat gaatttccat atgattctaa gattcctttg ccgataatta      1800
     taggattcct ttctgttcac ttctatttat ataataaagt ggtgcagagt aagcgcccta      1860
     taaggagaga gagagcttat caattgtatc atatggattg tcaacgccct acactcttgc      1920
     gatcgcttca atatgcatat tactataaac gatatatgtt ttttttttt                  1969
//