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EBI DbfetchID U10186; SV 1; circular; genomic DNA; STD; PRO; 2004 BP. XX AC U10186; XX DT 07-AUG-1994 (Rel. 40, Created) DT 18-OCT-2008 (Rel. 97, Last updated, Version 8) XX DE Escherichia coli isolate BL26A plasmid pLMO226, complete sequence. XX KW . XX OS Escherichia coli OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacteriales; OC Enterobacteriaceae; Escherichia. OG Plasmid pLMO226 XX RN [1] RP 1-2004 RA Sundstroem L.; RT "The genes of trimethoprim resistance and their recombinational RT dissemination"; RL Thesis (1989), Pharm. Biosciences, Div. of Microbiolog, Uppsala University XX RN [2] RP 1-2004 RX DOI; 10.1016/0378-1119(94)00905-8 RX PUBMED; 7867952. RA Sundstrom L., Jansson C., Bremer K., Heikkila E., Olsson-Liljequist B., RA Skold O.; RT "A new dhfrVIII trimethoprim-resistance gene, flanked by IS26, whose RT product is remote from other dihydrofolate reductases in parsimony RT analysis"; RL Gene 154(1):7-14(1995). XX RN [3] RP 1-2004 RA Sundstroem L.; RT ; RL Submitted (31-MAY-1994) to the EMBL/GenBank/DDBJ databases. RL Lars Sundstroem, Pharm. Biosciences, Div. of Microbiology, Uppsala, RL University, Uppsala, S-751 23, Sweden XX FH Key Location/Qualifiers FH FT source 1..2004 FT /organism="Escherichia coli" FT /plasmid="pLMO226" FT /isolate="BL26A" FT /mol_type="genomic DNA" FT /db_xref="taxon:562" FT gene join(1909..2004,1..615) FT /gene="tnp" FT CDS join(1909..2004,1..615) FT /codon_start=1 FT /transl_table=11 FT /gene="tnp" FT /product="transposase" FT /note="IS26 module" FT /db_xref="GOA:Q46714" FT /db_xref="InterPro:IPR001584" FT /db_xref="InterPro:IPR012337" FT /db_xref="UniProtKB/TrEMBL:Q46714" FT /protein_id="AAA68494.1" FT /translation="MNPFKGRHFQRDIILWAVRWYCKYGISYRELQLQEMLAERGVNVD FT HSTIYRWVQRYAPEMEKRLRWYWRNPSDLCPWHMDETYVKVNGRWAYLYRAVDSRGRTV FT DFYLSSRRNSKAAYRFLGKILNNVKKWQIPRFINTDKAPAYGRALALLKREGRCPSDVE FT HRQIKYRNNVIECDHGKLKRIIGATLGFKSMKTAYATIKGIEVMRALRKGQASAFYYGD FT PLGEMRLVSRVFEM" FT repeat_region 1..2004 FT /mobile_element="transposon:Tn5091" FT repeat_region 1..667 FT /mobile_element="insertion sequence:IS26" FT /note="flanking module of Tn5091" FT repeat_region 654..667 FT /rpt_type=TERMINAL FT gene 699..1220 FT /gene="dhfrVIII" FT RBS 699..702 FT /gene="dhfrVIII" FT CDS 711..1220 FT /codon_start=1 FT /transl_table=11 FT /gene="dhfrVIII" FT /product="dihydrofolate reductase type VIII" FT /function="trimethoprim resistance" FT /db_xref="GOA:P0ABQ7" FT /db_xref="InterPro:IPR001796" FT /db_xref="InterPro:IPR012259" FT /db_xref="InterPro:IPR017925" FT /db_xref="UniProtKB/Swiss-Prot:P0ABQ7" FT /citation=[1] FT /citation=[2] FT /protein_id="AAA68493.1" FT /translation="MIELHAILAATANGCIGKDNALPWPPLKGDLARFKKLTMGKVVIM FT GRKTYESLPVKLEGRTCIVMTRQALELPGVRDANGAIFVNNVSDAMRFAQEESVGDVAY FT VIGGAEIFKRLALMITQIELTFVKRLYEGDTYVDLAEMVKDYEQNGMEEHDLHTYFTYR FT KKELTE" FT repeat_region 1844..1857 FT /rpt_type=TERMINAL FT repeat_region 1846..2004 FT /mobile_element="insertion sequence:IS26" FT /note="flanking module of Tn5091" FT RBS 1899..1902 FT /gene="tnp" XX SQ Sequence 2004 BP; 489 A; 527 C; 525 G; 463 T; 0 other; ctgcaggaga tgctggctga acgcggagtg aatgtcgatc actccacgat ttaccgctgg 60 gttcagcgtt atgcgcctga aatggaaaaa cggctgcgct ggtactggcg taacccttcc 120 gatctttgcc cgtggcacat ggatgaaacc tacgtgaagg tcaatggccg ctgggcgtat 180 ctgtaccggg ccgtcgacag ccggggccgc actgtcgatt tttatctctc ctcccgtcgt 240 aacagcaaag ctgcataccg gtttctgggt aaaatcctca acaacgtgaa gaagtggcag 300 atcccgcgat tcatcaacac ggataaagcg cccgcctatg gtcgcgcgct tgctctgctc 360 aaacgcgaag gccggtgccc gtctgacgtt gaacaccgac agattaagta ccggaacaac 420 gtgattgaat gcgatcatgg caaactgaaa cggataatcg gcgccacgct gggatttaaa 480 tccatgaaga cggcttacgc caccatcaaa ggtattgagg tgatgcgtgc actacgcaaa 540 ggccaggcct cagcatttta ttatggtgat cccctgggcg aaatgcgcct ggtaagcaga 600 gtttttgaaa tgtaaggcct ttgaataaga caaaaggctg cctcatcgct aactttgcaa 660 cagtgcccga cgcaataatc accaattcct cttttgatag gtaaacaaca atgatcgagc 720 ttcatgccat tttagctgcc accgccaatg gttgcattgg gaaggacaac gcacttccct 780 ggccaccact aaaaggcgat ctggccagat tcaaaaaatt gaccatgggg aaggtggtca 840 ttatggggcg caagacctat gagagcttgc ccgtcaaatt agaaggtcgc acctgcatcg 900 ttatgacgcg ccaagcgctg gagcttccgg gtgttcgtga cgctaacggc gctatcttcg 960 tgaacaacgt cagcgacgcc atgcggttcg ctcaagaaga gagcgtgggc gatgtggcct 1020 acgtcattgg tggcgctgag atattcaagc gacttgcctt gatgatcacg cagattgaat 1080 tgacctttgt taagcgactg tacgaaggcg acacctacgt tgatctggcc gaaatggtca 1140 aagactacga gcagaatggc atggaagaac atgaccttca cacttacttc acttaccgta 1200 aaaaggagct tacagaatga ccttcgttgc catttcagat acgcacctgc acaactggtc 1260 gcagttcgcg ataccaacag agtcgggcat taactccagg ctgttgcaga ttcttaaagc 1320 cattgaagaa gcggcttgcg ccgcgattac cacgccccag cgggcgtggt tccaaccgtc 1380 tatcacggcg gcgatctctt ccatgtgcga ggttctttga ccccttctgt gctaaacgcg 1440 gtgctcgact tttttaagac catccaccgt gactacggcg ttcgctttcg catgatcgcc 1500 ggcaatcacg atctggaaac caaagactca tgtcctatgg gcaatgcagc tgcggcgctt 1560 aattcgttgc cgtttgtgga ggttgtatcc gaaaaaacgc tgttcgagga ccacaaagtc 1620 gctctgctgc catggcgcga ttccatggac gatctgcgtg cggatcttgc gcacgttaaa 1680 gacgcgatcg gcgctagcgt tgcctctaag tggacagcaa tcatccacgc accggttaac 1740 ggggttgttc ttggcattcc agaccatggg ttcgacggaa aagagctcgc ctcctatggc 1800 ttcgggttag tgctcgccgg tcactaccac aaccacaaaa aaatcggcac tgttgcaaat 1860 agtcggtggt gataaactta tcatcccctt ttgctgatgg agctgcacat gaacccattc 1920 aaaggccggc attttcagcg tgacatcatt ctgtgggccg tacgctggta ctgcaaatac 1980 ggcatcagtt accgtgagct gcag 2004 // |
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