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EBI DbfetchID U09865; SV 1; linear; genomic DNA; STD; PRO; 7291 BP. XX AC U09865; XX DT 26-SEP-1994 (Rel. 41, Created) DT 17-APR-2005 (Rel. 83, Last updated, Version 4) XX DE Alcaligenes eutrophus pyruvate dehydrogenase (pdhA), dihydrolipoamide DE acetyltransferase (pdhB), dihydrolipoamide dehydrogenase (pdhL), and ORF3 DE genes, complete cds. XX KW . XX OS Cupriavidus necator OC Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales; OC Burkholderiaceae; Cupriavidus. XX RN [1] RP 1-7291 RX PUBMED; 8021225. RA Hein S., Steinbuchel A.; RT "Biochemical and molecular characterization of the Alcaligenes eutrophus RT pyruvate dehydrogenase complex and identification of a new type of RT dihydrolipoamide dehydrogenase"; RL J. Bacteriol. 176(14):4394-4408(1994). XX RN [2] RP 1-7291 RA Hein S.; RT ; RL Submitted (19-MAY-1994) to the EMBL/GenBank/DDBJ databases. RL Silke Hein, Institut fuer Mikrobiologie, Grisebachstrasse 8, Goettingen RL 37077, Germany XX DR CABRI; DSM 428. XX FH Key Location/Qualifiers FH FT source 1..7291 FT /organism="Cupriavidus necator" FT /strain="H16 (DSM 428)" FT /mol_type="genomic DNA" FT /db_xref="taxon:106590" FT RBS 11..16 FT /gene="pdhA" FT /inference="non-experimental evidence, no additional FT details recorded" FT CDS 22..2709 FT /codon_start=1 FT /transl_table=11 FT /gene="pdhA" FT /product="pyruvate dehydrogenase" FT /EC_number="1.2.4.1" FT /db_xref="GOA:Q59097" FT /db_xref="InterPro:IPR004660" FT /db_xref="InterPro:IPR005474" FT /db_xref="InterPro:IPR009014" FT /db_xref="InterPro:IPR015941" FT /db_xref="UniProtKB/Swiss-Prot:Q59097" FT /protein_id="AAA21598.1" FT /translation="MSAVPEQILGASSANDADPQETHEWLDALQGVLAAEGPARAAFLI FT DKQIEYARVNGVTQPFHAETQYINTIPVEQQARIPGDQDIEHRIRSYTRWNAMAMVLRA FT NKHTNVGGHISSFASAATLYDVGYNHFWRAPSEAGGGDLVFVQGHSAPGVYSRAFLLGR FT LTQDQLDNFRQEVDGKGISSYPHPWLMPDFWQFPTVSMGLGPIMAIYQARFMKYLDSRG FT LAKAGDRKVWAFLGDGETDEPESLGAIGMAGREKLDNLVFVINCNLQRLDGPVRGNGKI FT IQELESEFRGAGWNVIKVVWGSKWDSLLARDTKGLLMKRMMECVDGEYQTMKAKDGAYV FT REHFFNTPELKAMVADWSDDDIWRLNRGGHDPHKIYAAYKAASEHKGQPTLILAKTIKG FT YGMGDAGQAMNVAHQQKKMPVDAIRKFRDQFNLPVADDQLEEVPYITFPEGSKELEYMR FT QARQNLGGYLPARRQKAEALPVPQLSAFDALLKATGEGREVSTTMAFVRILNTLLKDKQ FT IGKHVVPIVPDESRTFGMEGLFRQVGIWNQEGQKYVPEDHDQLMFYKESQTGQVLQEGI FT NEAGAMCDWIAAATSYSTHGVQMIPFYIYYSMFGIQRIGDLCWAAADMRSRGFLLGGTA FT GRTTLNGEGLQHEDGHSHVFHAAIPNCISYDPTFQYELAVVMQDGLRRMYAEQEDVYYY FT LTVMNENYEHPEMPAGVEQDIVKGMYQFRKGVENSNAPRVQLLGSGTIFREVIAAADLL FT KKDWGVESDLWGCPSFTELAREGHDVERFNLLHPTETPRESHVAKSLKSARGPVIASTD FT YVRAFAEQIRPFVPRRYVVLGTDGFGRSDTREKLRHFFEVDRYWVTLAALKALADEGAI FT GRDKVAEAIKKYNLDPNKPNPMSV" FT RBS 2865..2870 FT /gene="pdhB" FT /inference="non-experimental evidence, no additional FT details recorded" FT CDS 2877..4538 FT /codon_start=1 FT /transl_table=11 FT /gene="pdhB" FT /product="dihydrolipoamide acetyltransferase" FT /EC_number="2.3.1.12" FT /db_xref="GOA:Q59098" FT /db_xref="InterPro:IPR000089" FT /db_xref="InterPro:IPR001078" FT /db_xref="InterPro:IPR003016" FT /db_xref="InterPro:IPR004167" FT /db_xref="InterPro:IPR006256" FT /db_xref="InterPro:IPR011053" FT /db_xref="UniProtKB/Swiss-Prot:Q59098" FT /protein_id="AAA21599.1" FT /translation="MSQAIEIKVPDIGDYDAVPVIEVHVKPGDSINAEDALVTLESDKA FT TMDVPSPQAGVVKDVRIKVGDNVSEGSVLVMLEAANEPAAAPAPAAAAPAPAAAAPAPA FT PAPAAAPAAAPAAGGGGTIEVKVPDIGDYDAVPVIEVHVKAGDTINAEDAVVTLESDKA FT TMDVPSPQGGVVKEVKVKVGDNVAEGTLLLILEGAAAAAAPAAAAAAPAPAASAPAPAP FT APAAAASGTGRSTCCRAGRRWRNRQGRPRQPLGAQVRTRAGRGRVARAGHRPKGRITQE FT DVQGYVKGVMSGQAAAPAQAAAAGAGGGELGLLPWPKVDFTRFGEVESKALSRIKKISG FT ANLHRNWVMIPHVTNHDEADITELEAFRLQLNKENEKSGIKVTMLAFMIKATVAALKKF FT PNFNASLDGDNLVLKKYFNIGFAADTPNGLVVPVIKDADKKGVLEISQEMSELAKLARD FT GKLKPDQMQGGCFSISSLGGLGGTYFTPIINAPEVAIMGVCKSYQKPVWDGKQFAPRLT FT LPLSLSWDHRVIDGAEAARFNTYFGQLLADFRRILL" FT RBS 4601..4604 FT /note="for ORF3" FT CDS 4611..5288 FT /codon_start=1 FT /transl_table=11 FT /note="ORF3" FT /db_xref="GOA:Q44000" FT /db_xref="InterPro:IPR001353" FT /db_xref="UniProtKB/TrEMBL:Q44000" FT /protein_id="AAA21601.1" FT /translation="MTTCVVVRKGAEVAIAADALVTFGDTRLSRAYERNQKVFPVGDGF FT IALAGTTAHFPVMRTLLAGLGEECRLGSRDDVFRTFLKVHEKLKSDYFVNTKEDEDDPY FT ESSQIVCLIANPAGIFGVYSYREVFSFDRFWGIGSGRNYALGAMHAVYDRPDLDAGEIA FT RIGVDAGVEFDKSSAGPIEVHTVRLHEGDTAPAPPAGGATGGNPATGSTAPDSAATNND FT GAP" FT RBS 5312..5316 FT /gene="pdhL" FT /inference="non-experimental evidence, no additional FT details recorded" FT CDS 5322..7106 FT /codon_start=1 FT /transl_table=11 FT /gene="pdhL" FT /product="dihydrolipoamide dehydrogenase" FT /EC_number="1.8.1.4" FT /db_xref="GOA:Q59099" FT /db_xref="HSSP:1OJT" FT /db_xref="InterPro:IPR000089" FT /db_xref="InterPro:IPR000815" FT /db_xref="InterPro:IPR001327" FT /db_xref="InterPro:IPR003016" FT /db_xref="InterPro:IPR004099" FT /db_xref="InterPro:IPR006258" FT /db_xref="InterPro:IPR011053" FT /db_xref="InterPro:IPR012999" FT /db_xref="InterPro:IPR013027" FT /db_xref="InterPro:IPR016156" FT /db_xref="UniProtKB/TrEMBL:Q59099" FT /protein_id="AAA21600.1" FT /translation="MSVIEVKVPDIGDFDAVEVIEVLVKAGDTVEVEQSLIVLESDKAS FT MDVPSSAAGKVVEVKVKVGDKVGQGAVICTIEAQQAAAAPAPAQAPAPAQAPAPAAAAP FT APAPAAASHSGGADIQCEMLVLGAGPGGYSAAFRAADLGMNTVLVERYSTLGGVCLNVG FT CIPSKALLHNAAVIDEAKALAAHGILFGEAKIDLDGLRHYKNQVVGKLTGGLAGMAKAR FT KVQVVRGIGNFLDPHHMEVELTEGEGKRSTGKKTVIRFEKAIIAAGSQAVKLPFIPEDP FT RIVDSTGALELPEVPNKMLVIGGGIIGLEMATVYSTLGADIDVVEMLDGLMNGADRDLV FT KVWEKKNKDRFGKVMLKTKTVGVEAKPDGIYVKFEGEAAPAEPQRYDLVLVSVGRSPNG FT KRISAEKAGVAVSERGFINVDKQMRTNVPHIFAIGDIVGQPMLAHKAVHEAHVAAEAAH FT GEKAYFDAKQIPSVAFTDPEVAWAGLTEDECKEKGIKYSKGVFPWAASGRAIANGRDEG FT FTKLIFDEETHRVIGGGIVGTHAGDLISEVCLAIEMGADAVDIGKTIHPHPTLGESIGM FT AAEIYEGTCTDVPPPRKR" XX SQ Sequence 7291 BP; 1380 A; 2414 C; 2438 G; 1059 T; 0 other; gaattcaccc aggagacagt catgtccgcc gtaccagagc agattctcgg cgcctcgagc 60 gccaacgacg ccgatcccca ggaaacccat gaatggctcg acgccctgca gggcgtcctg 120 gccgccgaag gtccggcgcg cgccgctttc ctgatcgaca agcagatcga atacgcacgc 180 gtgaacggcg tgacccagcc gttccacgcc gagacgcagt acatcaacac gattccggtc 240 gagcagcagg cccgcatccc cggcgaccag gacatcgagc accggatccg ctcgtacacc 300 cgctggaacg ccatggcgat ggtgctgcgc gccaacaagc acaccaacgt cggcggccac 360 atctcgtcgt tcgcctcggc ggccacgctg tatgacgtcg gctacaacca cttctggcgc 420 gccccgtcgg aagccggcgg cggcgacctg gtgttcgtgc agggccactc ggctccgggc 480 gtgtattcgc gcgccttcct gctcggccgc ctgacccagg accagctcga caacttccgc 540 caggaggtgg acggcaaggg catctcgtcc tacccgcacc cgtggctgat gccggacttc 600 tggcagttcc cgacggtgtc gatgggcctg ggcccgatca tggccatcta ccaggcccgc 660 ttcatgaagt acctggacag ccgcggcctg gccaaggccg gcgaccgcaa ggtctgggcc 720 ttcctgggcg acggcgagac cgacgagccg gaatcgctgg gtgcgatcgg catggccggc 780 cgcgagaagc tcgacaacct ggtcttcgtc atcaactgca acctccagcg cctggatggc 840 ccggtgcgcg gcaacggcaa gatcatccag gaactggaat ccgaattccg cggtgccggc 900 tggaacgtga tcaaggtggt gtggggcagc aagtgggatt cgctgctggc gcgcgacacc 960 aagggcctgc tgatgaagcg catgatggaa tgcgtcgacg gcgagtacca gaccatgaag 1020 gccaaggacg gcgcctatgt ccgcgagcac ttcttcaaca cgcctgagct gaaggcgatg 1080 gtggccgact ggtccgacga cgacatctgg cgcctgaacc gcggcggcca cgatccgcac 1140 aagatctacg ccgcctacaa ggccgccagc gagcacaagg gccagcccac gctgatcctg 1200 gccaagacca tcaagggcta tggcatgggc gacgccggcc aggccatgaa cgtggcccac 1260 cagcagaaga agatgccggt ggacgcgatc cgcaagttcc gcgaccagtt caatcttccc 1320 gttgccgacg accagctcga agaggtgccg tacatcacct tcccggaagg ttcgaaggaa 1380 ctggaataca tgcgccaggc gcggcagaac ctgggcggct acctgccggc ccgccgccag 1440 aaggccgaag cgctgccggt cccgcagctg tccgcgttcg acgcgctgct gaaggccacc 1500 ggcgaaggcc gcgaagtgtc caccaccatg gccttcgtgc ggatcctgaa cacgctgctg 1560 aaagacaagc agatcggcaa gcacgtggtg cccatcgtgc cggacgagtc gcgcaccttc 1620 ggcatggaag gcctgttccg ccaggtcggc atctggaacc aggaaggcca gaagtacgtg 1680 ccggaagacc atgaccagct gatgttctac aaggaatcgc agactggcca ggtgctgcag 1740 gagggcatca acgaagcagg cgccatgtgc gactggatcg cggccgccac gtcgtactcg 1800 acgcacggcg tgcagatgat cccgttctac atctactact cgatgttcgg catccagcgt 1860 atcggcgacc tgtgctgggc cgctgccgac atgcgctcgc gcggcttcct gctgggcggc 1920 accgccggcc gcaccacgct gaacggcgaa ggcctgcagc atgaagacgg ccactcgcac 1980 gtgttccacg ccgcgatccc gaactgcatc tcgtacgacc cgaccttcca gtacgaactg 2040 gcggtggtca tgcaggacgg cctgcgccgc atgtacgccg agcaggaaga cgtctactac 2100 tacctgacgg tgatgaacga gaactacgag catccggaaa tgccggctgg cgtggagcag 2160 gacatcgtca agggcatgta ccagttccgc aagggtgtcg agaacagcaa cgcgccgcgc 2220 gtgcagctgc tgggctcggg cacgatcttc cgcgaggtga tcgccgctgc cgacctgctc 2280 aagaaggact ggggcgtgga gtcggacctg tggggctgcc cgagcttcac cgaactggcc 2340 cgcgaaggcc acgacgtcga gcgcttcaac ctgctgcacc cgaccgagac cccgcgcgaa 2400 tcgcacgtgg ccaagagcct gaagtcggcc cgcggcccgg tgatcgcctc caccgactac 2460 gtgcgtgcgt ttgccgagca gatccgtccg ttcgtgccgc gccgctacgt ggtgctgggc 2520 accgacggct tcggccgctc ggacacgcgc gagaagctgc gccacttctt tgaagtggac 2580 cgctactggg tcacgctggc cgcgctcaag gcgctggccg acgagggtgc gatcggccgc 2640 gacaaggttg ccgaggccat caagaagtac aacctcgacc cgaacaagcc caacccgatg 2700 tcggtctgat ccggcagcca gcaccaaccc ccgcagtacg gcaacgccgt gccgcgggcg 2760 ccccggccag agcccggacg gcgccttgcg gcatggcggc gtgacagaag cgggcggcga 2820 aggtaatctc gccgcttgcg gatgtcgcgc gtgccggcct gcccaggaga cactgaatga 2880 gtcaagcgat tgaaatcaag gtgccggata tcggcgacta tgacgccgtt cccgtcatcg 2940 aagtgcatgt gaaacccggc gacagcatca acgcggaaga cgcgctggtg acgctggaat 3000 cggacaaggc caccatggac gtgccctcgc cgcaggccgg cgtggtcaag gacgtccgca 3060 tcaaggtggg cgacaacgtg tccgaaggct cggtgctggt gatgctggag gcggccaacg 3120 agcccgccgc cgcacctgcc cctgctgccg cagcaccggc gccggccgcc gcagcccccg 3180 caccggctcc tgcccccgca gcagccccag ccgcggcccc ggccgctggc ggtggcggca 3240 ccatcgaagt caaggtgccc gatatcggcg actacgacgc cgtgccggtc atcgaagtcc 3300 acgtcaaggc gggcgacacc atcaacgccg aagacgccgt ggtgacgctg gagtccgaca 3360 aggccaccat ggacgtgccg tcgccgcaag gcggcgtggt caaggaagtc aaggtcaagg 3420 tcggtgacaa cgtcgccgaa ggcacgctgc tgctgatcct ggaaggcgcc gccgcagcag 3480 ccgccccggc agctgccgcc gcggcaccgg ctccggctgc cagcgcgccc gcaccggcac 3540 cggcccccgc agccgccgcc tccggcaccg gccgcagcac ctgctgccgc gccggccgcc 3600 gctggcgtaa ccggcaaggc cgcccacgcc agcccctcgg tgcgcaagtt cgcacgcgag 3660 ctgggcgtgg acgtgtcgcg cgtgccgggc accgccccaa gggccgtatc acccaggaag 3720 acgtgcaggg ctacgtcaag ggcgtgatga gcggccaggc cgccgcgccc gcacaggccg 3780 ccgcggctgg cgccggtggt ggcgagctcg gcctgctgcc gtggccgaag gtcgatttca 3840 cccgcttcgg cgaggtcgaa agcaaggccc tgtcgcgcat caagaagatc tcgggtgcca 3900 acctgcaccg caactgggtc atgatcccgc acgtcaccaa ccatgacgaa gcggacatca 3960 ccgagctgga agccttccgc ctgcagctga acaaggaaaa cgagaagtcc ggcatcaagg 4020 tgacgatgct ggcgttcatg atcaaggcca cggtggcggc gctgaagaag ttcccgaact 4080 tcaacgcgtc gctcgatggt gacaacctcg tgctgaagaa gtacttcaac atcggttttg 4140 ccgccgacac cccgaacggc ctggtcgtgc cggtgatcaa ggacgccgac aagaagggcg 4200 tgctggagat cagccaggaa atgagcgagc tcgccaagct ggcccgcgac ggcaagctca 4260 agccggacca gatgcagggc ggctgcttct cgatctcttc gctgggcggc ctcggcggca 4320 catacttcac gccgatcatc aacgcgccgg aagtggccat catgggcgtg tgcaagtcgt 4380 accagaagcc ggtgtgggac ggcaagcagt tcgccccgcg cctgacgctg ccgctgtcgc 4440 tgtcgtggga ccaccgcgtc atcgacggtg ccgaagcggc gcgcttcaac acgtacttcg 4500 ggcaactgct ggcggatttc cgccgcatcc tgctgtaagc ggggttgcgc cgggcgggca 4560 ggccgacggc ctggcctgcc cggcgcacca cgcgcaaccg ggagcgagcc atgactacct 4620 gcgtggtcgt gaggaagggc gccgaggtgg ccatcgcggc agatgcgctg gtcacctttg 4680 gcgacacgcg cctgtcgcgc gcctacgagc gcaaccagaa ggtgttcccg gtaggcgacg 4740 gcttcatcgc gctggccggg accaccgcgc acttcccggt catgcgcacg ctgctggcgg 4800 ggcttggcga agagtgccgg ctgggctcgc gtgatgacgt gttccgcacc ttcctgaagg 4860 tgcatgagaa gctgaagtcg gactacttcg tcaacaccaa ggaagacgag gacgatccct 4920 acgagtcgtc gcagatcgta tgcctgatcg ccaatccggc gggcatcttc ggcgtctact 4980 cgtaccgcga ggtgttttcg ttcgaccgct tctggggtat cggctcgggc cgcaactacg 5040 cgctgggcgc gatgcatgcg gtctatgacc ggccggacct ggatgcgggc gagatcgcgc 5100 gcatcggcgt cgatgcgggc gtggagttcg acaagagttc ggcggggccg atcgaggtgc 5160 acaccgtgcg gctgcacgag ggtgacaccg cgccggcacc gccggctggc ggtgccacgg 5220 gcggcaatcc ggcaaccggc agtacggcac ccgacagcgc ggcaaccaac aacgacggcg 5280 cgccctgaag ccgagaggcg ggcgcgcata gaggagcaaa catgagtgtg atcgaagtca 5340 aggtgccgga tatcggcgat ttcgacgcgg tggaagtgat cgaggtgctg gtcaaggccg 5400 gcgacacggt cgaggtggaa cagtccctga tcgtgctgga atccgacaag gccagcatgg 5460 acgtgccgtc gtcggccgcc ggcaaggtgg ttgaggtcaa ggtcaaggtc ggcgacaagg 5520 tcggccaggg cgcggtgatc tgcaccatcg aggcccagca ggccgcggcc gccccggcgc 5580 ccgcgcaagc tccggcaccg gcgcaggctc ccgcacccgc ggcggcggct ccggcaccgg 5640 ctcctgctgc cgccagccac agcggtggcg ccgatatcca gtgcgagatg ctggtgctgg 5700 gcgccggccc cggcggctac tcggccgcct tccgcgccgc agacctgggc atgaataccg 5760 tgctggtgga gcgctacagc acgcttggcg gcgtctgcct gaacgtgggc tgcatcccgt 5820 ccaaggcgct gctgcacaac gccgccgtga tcgacgaagc caaggcgctg gccgcccacg 5880 gcatcctgtt cggcgaagcc aagatcgacc tggacggcct gcgccactac aagaaccagg 5940 tggtcggcaa gctcaccggc ggcctggccg gcatggccaa ggcgcgcaag gtgcaggtgg 6000 tgcgcggcat cggcaacttc ctcgacccgc atcacatgga ggtcgagctg accgaaggcg 6060 agggcaagcg cagcaccggc aagaagaccg tgatccgctt cgagaaggcc atcatcgctg 6120 ccggcagcca ggcggtgaaa ctgccgttca tcccggaaga tccgcgcatc gtcgactcga 6180 ccggcgcgct ggagctgccc gaagtgccca acaagatgct ggtcatcggc ggcggcatca 6240 tcggcctgga aatggccacg gtgtacagca cgctgggtgc cgacatcgat gtcgtggaaa 6300 tgctcgacgg cctgatgaac ggtgctgacc gcgacctggt caaggtctgg gaaaagaaga 6360 acaaggaccg cttcggcaag gtcatgctga agaccaaaac cgtcggcgtg gaagccaaac 6420 cggacggcat ctacgtcaag ttcgaaggcg aggccgcgcc ggccgagcca cagcgctacg 6480 acctggtgct ggtgtcggtg ggccgctcgc ccaacggcaa gcgcatcagc gcagagaagg 6540 ccggcgtggc cgtgagcgag cgcggcttta tcaacgtcga caagcagatg cgcaccaatg 6600 tgccgcatat ctttgcgatc ggcgatatcg tcggccagcc gatgctggcg cacaaggcgg 6660 tgcatgaagc ccacgttgcc gctgaggccg cgcatggcga gaaggcttac ttcgatgcca 6720 agcagatccc gtcggtggcc ttcaccgatc cggaagtggc ctgggccggc ctgaccgaag 6780 acgagtgcaa ggagaagggg atcaagtaca gcaagggcgt gttcccctgg gcggcatcgg 6840 gccgcgccat cgccaacggc cgcgacgaag gcttcaccaa gctgatcttc gacgaggaaa 6900 cccatcgcgt gatcggcggc ggcatcgtcg gcacgcatgc aggcgacctg atcagcgagg 6960 tctgcctggc gatcgagatg ggcgccgatg cggtggatat cggcaagacc atccatccgc 7020 acccgacgct gggtgaatcg atcggcatgg cggcggagat ctatgaaggc acgtgtactg 7080 acgtgccgcc gccgcgcaag cgctgagcgc cgttcgctgc aagcgagaaa gcccgcctgc 7140 ccaggcgggc tttttcattg ccaattatgc aattaaccgc cagaatttgc cggcgtccgc 7200 gaggtctgcc tgccagtacc gctcgcgtcg gcagccaagg tgcagattgc gacaggagcg 7260 gtgcaagacc ttcgctgcct atactgaatt c 7291 // |
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