 |
|
EBI DbfetchID U09771; SV 1; linear; genomic DNA; STD; PRO; 13128 BP. XX AC U09771; XX DT 25-MAY-1994 (Rel. 39, Created) DT 17-APR-2005 (Rel. 83, Last updated, Version 13) XX DE Citrobacter freundii cyclopropane fatty acid synthase (cfa) gene, partial DE cds; dihydroxyacetone kinase (dhaK), glycerol dehydrogenase (dhaD), DE transcriptional activator (dhaR), glycerol dehydratase reactivation factor DE small subunit (dhaG), 1,3-propanediol dehydrogenase (dhaT), glycerol DE dehydratase 60kDa subunit (dhaB), glycerol dehydratase 21kDa subunit DE (dhaC), glycerol dehydratase 16kDa subunit (dhaE), and glycerol dehydratase DE reactivation factor large subunit (dhaF) genes, complete cds; and unknown DE genes. XX KW . XX OS Citrobacter freundii OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacteriales; OC Enterobacteriaceae; Citrobacter. XX RN [1] RP 1-13128 RX PUBMED; 7721705. RA Daniel R., Boenigk R., Gottschalk G.; RT "Purification of 1,3-propanediol dehydrogenase from Citrobacter freundii RT and cloning, sequencing, and overexpression of the corresponding gene in RT Escherichia coli"; RL J. Bacteriol. 177(8):2151-2156(1995). XX RN [2] RP 1-13128 RX PUBMED; 7635824. RA Daniel R., Stuertz K., Gottschalk G.; RT "Biochemical and molecular characterization of the oxidative branch of RT glycerol utilization by Citrobacter freundii"; RL J. Bacteriol. 177(15):4392-4401(1995). XX RN [3] RP 1-13128 RX PUBMED; 8824629. RA Seyfried M., Daniel R., Gottschalk G.; RT "Cloning, sequencing, and overexpression of the genes encoding coenzyme RT B12-dependent glycerol dehydratase of Citrobacter freundii"; RL J. Bacteriol. 178(19):5793-5796(1996). XX RN [4] RP 1-13128 RX DOI; 10.1046/j.1432-1327.2001.02123.x RX PUBMED; 11298756. RA Seifert C., Bowien S., Gottschalk G., Daniel R.; RT "Identification and expression of the genes and purification and RT characterization of the gene products involved in reactivation of coenzyme RT B12-dependent glycerol dehydratase of Citrobacter freundii"; RL Eur. J. Biochem. 268(8):2369-2378(2001). XX RN [5] RP 1-13128 RA Daniel R.; RT ; RL Submitted (16-MAY-1994) to the EMBL/GenBank/DDBJ databases. RL Institut fuer Mikrobiologie, Grisebachstrasse 8, Goettingen 37077, Germany XX DR CABRI; DSM 30040. XX CC On Mar 19, 1996 this sequence version replaced gi:493081. XX FH Key Location/Qualifiers FH FT source 1..13128 FT /organism="Citrobacter freundii" FT /strain="DSM 30040" FT /mol_type="genomic DNA" FT /db_xref="taxon:546" FT CDS <1..271 FT /codon_start=2 FT /transl_table=11 FT /gene="cfa" FT /product="cyclopropane fatty acid synthase" FT /EC_number="2.1.1.79" FT /db_xref="GOA:P45509" FT /db_xref="InterPro:IPR003333" FT /db_xref="UniProtKB/Swiss-Prot:P45509" FT /protein_id="AAB48842.1" FT /translation="ISHIAEASESRFVMEDWHNFGSDYDKTLMAWHERFNQAWPELSSR FT YSATFRRMFNYYLCACAGAFRARDIELWQVLFSRGVEGGIRVYR" FT CDS complement(337..1995) FT /codon_start=1 FT /transl_table=11 FT /gene="dhaK" FT /product="dihydroxyacetone kinase" FT /EC_number="2.7.1.29" FT /note="the initial methionine in the mature protein is FT removed" FT /db_xref="GOA:P45510" FT /db_xref="InterPro:IPR012734" FT /db_xref="PDB:1UN8" FT /db_xref="UniProtKB/Swiss-Prot:P45510" FT /experiment="experimental evidence, no additional details FT recorded" FT /protein_id="AAB48843.1" FT /translation="MSQFFFNQRTHLVSDVIDGAIIASPWNNLARLESDPAIRIVVRRD FT LNKNNVAVISGGGSGHEPAHVGFIGKGMLTAAVCGDVFASPSVDAVLTAIQAVTGEAGC FT LLIVKNYTGDRLNFGLAAEKARRLGYNVEMLIVGDDISLPDNKHPRGIAGTILVHKIAG FT YFAERGYNLATVLREAQYAASNTFSLGVALSSCHLPQETDAAPRHHPGHAELGMGIHGE FT PGASVIDTQNSAQVVNLMVDKLLAALPETGRLAVMINNLGGVSVAEMAIITRELASSPL FT HSRIDWLIGPASLVTALDMKGFSLTAIVLEESIEKALLTEVETSNWPTPVPPREITCVV FT SSHASARVEFQPSANALVAGIVELVTATLSDLETHLNALDAKVGDGDTGSTFAAAAREI FT ASLLHRQQLPLNNLATLFALIGERLTVVMGGSSGVLMSIFFTAAGQKLEQGANVVEALN FT TGLAQMKFYGGADEGDRTMIDALQPALTSLLAQPKNLQAAFDAAQAGAERTCLSSKANA FT GRASYLSSESLLGNMDPGAQRLAMVFKALAESELG" FT RBS complement(2001..2006) FT /gene="dhaK" FT /note="putative" FT RBS 2544..2547 FT /gene="dhaD" FT /note="putative" FT CDS 2557..3654 FT /codon_start=1 FT /transl_table=11 FT /gene="dhaD" FT /product="glycerol dehydrogenase" FT /EC_number="1.1.1.6" FT /db_xref="GOA:P45511" FT /db_xref="HSSP:1JPU" FT /db_xref="InterPro:IPR016205" FT /db_xref="UniProtKB/Swiss-Prot:P45511" FT /experiment="experimental evidence, no additional details FT recorded" FT /protein_id="AAB48844.1" FT /translation="MLKVIQSPAKYLQGPDASTLFGQYAKNLADSFFVIADDFVMKLAG FT EKVLNGLHSHDISCHAERFNGECSHIEINRLIAILKQHGCRGVVGIGGGKTLDTAKAIG FT YYQKLPVVVIPTIASTDAPTSALSVIYTEAGEFEEYLIYPKNPDMVVMDTAIIAKAPVR FT LLVAGMGDALSTWFEAKACYDARATSMAGGQSTVAALSLARLCYDTLLAEGEKARFAAQ FT AGVVTDALERIVEANTYLSGIGFESSGLAGAHAIHNGFTILEECHHLYHGEKVAFGTLA FT QLVLQNSPMEEIETVLNFCQKVGLPVTLAEMGVKDDIDGKIMAVAKATCAEGETIHNMP FT FSVTPESVHAAILTADLLGQQWLAR" FT RBS 3736..3738 FT /gene="dhaR" FT /note="putative" FT CDS 3746..5671 FT /codon_start=1 FT /transl_table=11 FT /gene="dhaR" FT /product="transcriptional activator" FT /note="putative s54 interaction domain and helix-turn-helix FT motif" FT /db_xref="GOA:P45512" FT /db_xref="InterPro:IPR002078" FT /db_xref="UniProtKB/Swiss-Prot:P45512" FT /protein_id="AAB48845.1" FT /translation="MTTHTQDIGKEVSSVIAQSWHRCSKFMQRETWQAPHQAQGLTFES FT ICRRKTALLTIAQAALEDAWEFMDGRPCALLILDESACILSRCGDPQTVEQLAELGFRD FT GSYCAESIIGSCALSLATMPGQPTKTSGDQHFKQALHPWSFCSTPVFDNHGRLFGSISL FT CCLVEHECVSDLSLTLAIAREVGNSLLTDSLLAESNRHLNQMYGLLESMDDGVMAWNEQ FT GVLQFLNARAALLLHLDAQASQGKNINDLLNLPMLLRRAIKHARGLNHVEVTFESQHQF FT VDAVITLKPIVEEQGNSFILLLHPVEQMRQLMTSQLGKVSHTFEQMSTDDPETRRLIHF FT GRQAARGSFPILLCGEEGVGKELLSQAIHNESERASGPYIAVNCQLYANSVLGQDFMGS FT APTDDENGRLSRLELANGGTLFLEKIEYLAPELQSALLQVIKQGVLTRLDARRLIPVDV FT KVIATTTVDLANLVEQNRFSRQLYYALHSFEIVIPPLRARRNSIPSLIYTRLNSLKKRF FT SSSLKIDDDALAQLVAYSWPGNDFELNSIIENIAISSDNGHIRLSNLPDYLFAERPGLE FT TASSLLPASLTFTAIEKEAIIHAARVTSGRVQEMSQLLNIGRTTLWRKMKQYDIDASQF FT KRKHLE" FT CDS complement(5649..6179) FT /codon_start=1 FT /transl_table=11 FT /product="unknown" FT /note="orfW" FT /db_xref="GOA:P45515" FT /db_xref="InterPro:IPR002779" FT /db_xref="UniProtKB/Swiss-Prot:P45515" FT /protein_id="AAB48846.1" FT /translation="MYRIYTRTGDNGTTALFGGSRIDKDDIRVEAYGTVDELISQLGVC FT YASTRQAELRQELHAMQKMLFVLGAELASDQKGLTRLKQRIGEEDIQALEQLIDRNMAQ FT SGPLKEFVIPGKNLASAQLHVARTLTRRLERILIAMGRTLTLRDEARRYINRLSDALFS FT MARIEETTPDVCA" FT CDS complement(6180..6533) FT /codon_start=1 FT /transl_table=11 FT /gene="dhaG" FT /product="glycerol dehydratase reactivation factor small FT subunit" FT /db_xref="HSSP:1NBW" FT /db_xref="InterPro:IPR010254" FT /db_xref="UniProtKB/Swiss-Prot:P45516" FT /protein_id="AAB48847.1" FT /translation="MSLSSPGVHLFYHSRWQGTRVLDELCWGLEEQGVPCRAICCDDHD FT CALALGKLAAKSSTLRVGLGLNATGDIALTHAQLPEDRALVCGHTRAGTAQIRTLGANA FT GQLVKVLPFSEIK" FT RBS complement(6189..6191) FT /note="putative for orfW" FT RBS complement(6541..6543) FT /gene="dhaG" FT /note="putative" FT CDS complement(6550..7713) FT /codon_start=1 FT /transl_table=11 FT /gene="dhaT" FT /product="1,3-propanediol dehydrogenase" FT /EC_number="1.1.1.202" FT /db_xref="GOA:P45513" FT /db_xref="InterPro:IPR018211" FT /db_xref="UniProtKB/Swiss-Prot:P45513" FT /experiment="experimental evidence, no additional details FT recorded" FT /protein_id="AAB48848.1" FT /translation="MSYRMFDYLVPNVNFFGPNAISVVGERCKLLGGKKALLVTDKGLR FT AIKDGAVDKTLTHLREAGIDVVVFDGVEPNPKDTNVRDGLEVFRKEHCDIIVTVGGGSP FT HDCGKGIGIAATHEGDLYSYAGIETLTNPLPPIVAVNTTAGTASEVTRHCVLTNTKTKV FT KFVIVSWRNLPSVSINDPLLMLGKPAPLTAATGMDALTHAVEAYISKDANPVTDAAAIQ FT AIRLIARNLRQAVALGSNLKARENMAYASLLAGMAFNNANLGYVHAMAHQLGGLYDMPH FT GVANAVLLPHVARYNLIANPEKFADIAEFMGENTDGLSTMDAAELAIHAIARLSADIGI FT PQHLRDLGVKEADFPYMAEMALKDGNAFSNPRKGNEKEIAEIFRQAF" FT mat_peptide complement(6553..7710) FT /gene="dhaT" FT /product="unnamed" FT /note="1,3-propanediol dehydrogenase" FT RBS complement(7719..7722) FT /gene="dhaT" FT /note="putative" FT CDS complement(7736..8164) FT /codon_start=1 FT /transl_table=11 FT /transl_except=(pos:8162..8164,aa:Met) FT /product="unknown" FT /note="GTG start codon; orfY" FT /db_xref="InterPro:IPR005624" FT /db_xref="UniProtKB/Swiss-Prot:P45517" FT /protein_id="AAB48849.1" FT /translation="MNKSQQIATITLAAAKKMAQAVEAKALEINVPVVFSVVDHGGNTL FT LMQRMDDAFVTSCDISLNKAYTACCLRQGTHEITDAVQPGASLYGLQLTNQQRIVIFGG FT GLPVILNGKVIGAVGVSGGTVEQDRLLAETALDCFSEL" FT RBS complement(8170..8174) FT /note="putative for orfY" FT RBS 8543..8545 FT /gene="dhaB" FT /note="putative" FT CDS 8556..10223 FT /codon_start=1 FT /transl_table=11 FT /gene="dhaB" FT /product="glycerol dehydratase 60kDa subunit" FT /EC_number="4.2.1.30" FT /db_xref="GOA:P45514" FT /db_xref="HSSP:1IWP" FT /db_xref="InterPro:IPR003206" FT /db_xref="UniProtKB/Swiss-Prot:P45514" FT /experiment="experimental evidence, no additional details FT recorded" FT /protein_id="AAB48850.1" FT /translation="MRRSKRFEVLAQRPVNQDGLIGEWPEEGLIAMESPYDPASSVKVE FT NGRIVELDGKSRAEFDMIDRFIADYAINVPEAERAMQLDALEIARMLVDIHVSREEIIA FT ITTAITPAKRLEVMAQMNVVEMMMALQKMRARRTPSNQCHVTNLKDNPVQIAADAAEAG FT IRGFSEQETTVGIARYAPFNALALLVGSQCGAPGVLTQCSVEEATELELGMRGLTSYAE FT TVSVYGTESVFTDGDDTPWSKAFLASAYASRGLKMRYTSGTGSEALMGYSESKSMLYLE FT SRCIFITKGAGVQGLQNGAVSCIGMTGAVPSGIRAVLAENLIASMLDLEVASANDQTFS FT HSDIRRTARTLMQMLPGTDFIFSGYSAVPNYDNMFAGSNFDAEDFDDYNILQRDLMVDG FT GLRPVTEEETIAIRNKAARAIQAVFRELGLPLISDEEVDAATYAHGSKDMPARNVVEDL FT AAVEEMMKRNITGLDIVGALSSSGFEDIASNILNMLRQRVTGDYLQTSAILDRQFDVVS FT AVNDINDYQGPGTGYRISAERWAEIKNIAGVVQPGSIE" FT RBS 10222..10225 FT /gene="dhaC" FT /note="putative" FT CDS 10235..10819 FT /codon_start=1 FT /transl_table=11 FT /gene="dhaC" FT /product="glycerol dehydratase 21kDa subunit" FT /EC_number="4.2.1.30" FT /db_xref="GOA:Q59273" FT /db_xref="HSSP:1IWP" FT /db_xref="InterPro:IPR010254" FT /db_xref="UniProtKB/TrEMBL:Q59273" FT /experiment="experimental evidence, no additional details FT recorded" FT /protein_id="AAB48851.1" FT /translation="MECTTERKPVFTLQVSEGEAAKADERVDEVVIGVGPAFDKYQHKT FT LIDMPHKAILKELVAGIEEEGLHARVVRILRTSDVSFMAWDAANLSGSGIGIGIQSKGT FT TVIHQRDLLPLSNLELFSQAPLLTLETYRQIGKNAARYARKESPSPVPVVNDQMVRPKF FT MAKAALFHIKETKHVVQDRAPVTLHIALVRE" FT RBS 10811..10814 FT /gene="dhaE" FT /note="putative" FT CDS 10822..11250 FT /codon_start=1 FT /transl_table=11 FT /gene="dhaE" FT /product="glycerol dehydratase 16kDa subunit" FT /EC_number="4.2.1.30" FT /db_xref="GOA:Q59274" FT /db_xref="HSSP:1MMF" FT /db_xref="InterPro:IPR003207" FT /db_xref="UniProtKB/TrEMBL:Q59274" FT /experiment="experimental evidence, no additional details FT recorded" FT /protein_id="AAB48852.1" FT /translation="MNDNIMTAQDYPLATRCPEKIQTPTGKPLTEITLENVLAGRVGPQ FT DVRISQQTLEYQAQIAEQMQRHAVARNFRRAAELIAIPDARILEIYNALRPFRSSFAEL FT QAIADELEHTWHATVNAGFVRESAEVYLQRNKLRKGSQ" FT RBS 11253..11256 FT /gene="dhaF" FT /note="putative" FT CDS 11261..13072 FT /codon_start=1 FT /transl_table=11 FT /gene="dhaF" FT /product="glycerol dehydratase reactivation factor large FT subunit" FT /db_xref="HSSP:1NBW" FT /db_xref="InterPro:IPR009191" FT /db_xref="UniProtKB/TrEMBL:Q46035" FT /protein_id="AAB48853.1" FT /translation="MPLIAGIDIGNATTEVALAQHGRFIASGIVATTGMKGTRDNIAGV FT VASLQQALNNTPWSLQDVAKICINEAAPVIGDVAMETITETIITESTMIGHNPQTPGGV FT GVGMGTTIAVEKLAALSEDRFTQGWIPLVGEERDFLEAVWFINEALDRGVNVVAAILKK FT DDGVLVNNRLRRTMPVVDEVTLLEKVPEGVLAAVEVAAPGQVVRVLSNPYGIATFFTLT FT PEETQTIVPIARALIGNRSAVVLKTPQGDVRSRVIPAGKIFIRGEKRGGEADVAQGAQA FT IMQAMSTCAPVCDIRGEAGTHAGGMLERVRKVMASLTGHDMNAVYIQDLLAVDTFIPRK FT VQGGIAGECSMENAVGIAAMVKSDRLQMQAIASELSARLNIPVEVGGVEANMAVAGALT FT TPGCAAPLAILDLGAGSTDAAIINSDGVVKAVHLAGAGNMVSLLIQTELGLSDPFLAEE FT IKKYPLAKVESLFSIRHENGAVEFFREPLSPSVFAKVVYLKNGELIPVDNQTSLEKIRL FT VRRQAKEKVFVTNCLRALRQVSPGGSIRDMTFVVLVGGSSLDFEIPQMITDALAHYGVV FT AGQGNIRGTEGPRNAVATGLVLAGIAN" XX SQ Sequence 13128 BP; 2908 A; 3444 C; 3830 G; 2946 T; 0 other; gatctcccat attgccgaag ccagcgaatc acggtttgtg atggaagact ggcataactt 60 tggcagcgat tacgataaaa cgctaatggc gtggcacgag cgctttaatc aagcatggcc 120 ggagctgtca tcacgttatt ccgccacctt cagaagaatg tttaactact atttatgcgc 180 ctgcgccggg gctttccgcg ccagagatat tgaactttgg caggtactgt ttagtcgcgg 240 cgtagagggc gggatccgcg tgtatcgtta acgttctgcc tgataagcga agcgccatca 300 ggcagtaaat acctgagaag cgctccttgt tacatcttag cccagctcac tctccgctag 360 cgctttaaac accatcgcta ggcgctgcgc gccggggtcc atatttccga gcaggctttc 420 gctgctcaga tacgatgcgc gacccgcatt ggctttgctc gacaaacagg ttcgttcggc 480 tcccgcttgc gcggcgtcga atgcggcctg cagatttttc ggctgtgcga gcagcgaggt 540 cagggccggt tgcagcgcat caatcatcgt gcgatcgcct tcgtctgcgc cgccgtagaa 600 cttcatctgc gccagccccg tatttagcgc ttcgacaacg ttagcgccct gttccagttt 660 ctgcccggcg gcggtaaaga agattgacat cagcacaccg ctggaaccgc ccatcaccac 720 ggtcagacgt tcgccaatca gcgcgaacag cgtggcaagg ttattcagcg gcagctgctg 780 gcgatgcagc aggctggcaa tttcacgcgc cgcggcggca aaggtcgaac cggtatcgcc 840 atcgccgact ttggcgtcca gcgcattcag atgagtctcc agatcggaaa gggttgcggt 900 gaccagctcc acaatcccgg ccaccagggc gtttgccgaa ggctggaatt ccacgcgggc 960 gctagcgtga gatgacacta cgcaggtgat ttcacgcggt gggaccggcg tcggccagtt 1020 gctggtttcc acttcggtga gcagtgcttt ttcgatgctc tcttccagca cgatggccgt 1080 cagtgagaag cctttcatat ccagcgcggt gaccagcgag gccgggccaa ttagccagtc 1140 gatacgcgag tgcagcgggc tgctggcgag ttcgcgggtg atgatggcca tttcggccac 1200 ggaaacgccg ccaagattat taatcatcac cgccagacga ccggtttcag gcagggcggc 1260 cagcagttta tccaccatca ggtttaccac ttgcgcactg ttttgggtgt cgataaccga 1320 tgcgcctggt tcgccgtgaa ttcccatacc cagctccgca tgacccggat gatgacgagg 1380 ggctgcgtcg gtttcttgcg gcagatgaca gctggaaagc gctacgccca ggctaaaggt 1440 gttgctggct gcgtactgcg cttcacgcag gacggtggcg aggttatagc cgcgttcggc 1500 aaaatagcct gcgattttat gcaccaggat agttcccgca atgccacgtg ggtgtttgtt 1560 atccggcagg gagatgtcgt cgccgacaat cagcatttca acgttatagc caaggcgacg 1620 cgccttctcg gcggcgagac cgaaattaag acggtcaccg gtgtagtttt tcacaatcaa 1680 caaacagcca gcctcaccgg tcaccgcctg aatcgcggtc agtacagcat ccacgctcgg 1740 ggaggcgaaa acgtcgccgc agaccgcagc ggttagcatg cctttaccga taaacccaac 1800 gtgcgcgggt tcgtgtcccg aaccgccgcc ggaaatgacc gctacgttat ttttattaag 1860 gtcacgacgg accacgatgc gaatggccgg atcgctttcc agacgcgcca ggttattcca 1920 tgggctggcg ataatcgccc cgtcgatgac gtcgctcaca agatgggtgc gttggttaaa 1980 aaagaattga gacataacgg ctcctcgagg ttgagtgaaa gacaaacccc cgaatgtagg 2040 atcatggtgc ggaggcaatg aagagattct ggcgaagtca gcgtggaaaa aaacaacgct 2100 aaaatgagtt tcattacgga acgctgacgt tgttttttgt tccgaaatga aacttaaaaa 2160 gacggggaat gatcataatg cgagctcgct cgactttttt cttctgcgat cagcgccgct 2220 catacgcgca gccagccttt tttttgccgg ttttaataca actgcatgag taaaagagga 2280 actgagcttc gtgtttgccg gagatttggg ggtaaatgta cggatcggga tcgcaaacct 2340 tatcattttt gtggataagc gttccagaat ggaacgtgcg tcgtttttat ctgtttcata 2400 tcagaacaaa atgacgaaag atttttttgt tcacgcctcg ccatacagtt attgcatcgc 2460 tccggtacct cctgtttttg ccgacagcaa caggccaaac aggttaacgc cagggctcac 2520 catgtaaaaa catgtactta tttgaggatg aaaggaatgc taaaagttat tcaatctcca 2580 gccaaatatc tccagggccc tgacgcttcc accttatttg gtcaatacgc taaaaatctg 2640 gcggacagct ttttcgtgat tgcggatgac ttcgtgatga agctggcggg agagaaggtt 2700 ctcaatggcc tgcacagcca cgacattagc tgccacgcgg aacgcttcaa cggtgaatgc 2760 agccatattg aaattaatcg cctgattgcc attctgaaac agcacggttg ccgcggcgtg 2820 gttgggattg gcggcgggaa aacgttggat accgccaaag cgattggtta ctaccagaag 2880 ctaccggtgg tggtgatccc gactatcgcc tctaccgatg cgccaaccag cgcgctgtcc 2940 gttatctata ccgaagccgg tgagtttgaa gagtatctga tctacccgaa aaacccggat 3000 atggtggtga tggacaccgc aatcattgcc aaagcgccgg tacgcctgct ggtggccggg 3060 atgggcgatg cgctctcaac ctggtttgaa gcgaaagcct gttatgacgc cagagcgacc 3120 agcatggcgg gcggacagtc caccgtagcg gcgctgagcc tggcgcgcct atgctatgat 3180 accctgctgg cggaaggcga aaaggcacgc tttgccgcgc aggctggcgt ggtgactgat 3240 gcgctggagc gtattgtcga agcgaacact tacctcagcg gcatcggctt tgaaagcagc 3300 ggtcttgcag gggcgcatgc tatccacaac ggttttacga ttctggaaga gtgtcaccat 3360 ttgtaccacg gtgaaaaagt cgcctttggt acgctggcgc agctggtgct gcaaaacagc 3420 ccgatggaag agatcgaaac cgtgctgaat ttctgtcaga aagtaggcct gccggtaacg 3480 ttagcggaaa tgggcgtgaa agacgacatt gacggcaaga tcatggccgt ggccaaagcg 3540 acctgcgcag aaggtgaaac catccacaat atgccgttct cggtgacgcc tgaaagcgta 3600 catgccgcga tactgacggc agacctgctg ggacagcagt ggctggcgcg ttaatcagtc 3660 gtcggtaagt ttttatccgg catcttctcc cccctctgac agtctctgcc ggagggttgt 3720 ctatggtaaa acagcggaaa gggatatgac gacgcacact caggacattg ggaaggaagt 3780 ctcttcggtc attgcccagt catggcatcg ttgcagcaag tttatgcagc gggaaacctg 3840 gcaggcgccg catcaggcgc agggcctgac ctttgagtcg atctgtcgtc gcaaaacggc 3900 gctgctgacg attgctcagg ccgcgctgga agatgcctgg gagtttatgg acggccgtcc 3960 gtgcgcgttg ctaattctgg atgaatcagc ctgtattctc agccgttgcg gcgatccgca 4020 gacggtggag caactggcgg agctcggttt tcgcgacggc agctactgtg cagaaagcat 4080 tattggcagt tgcgcgttgt cgctggcgac gatgccgggg cagccgacaa aaacctctgg 4140 cgatcaacac tttaaacagg cgttgcatcc gtggtcgttc tgctctacgc cagtattcga 4200 taaccatggt cgactgtttg gctccatctc gctgtgctgt ctggtagagc atgagtgcgt 4260 ttctgattta tccctgacgc tggcgattgc cagagaagtc ggcaactcat tgctgactga 4320 cagcctgctg gctgaatcca accgccatct gaaccagatg tacgggctgc tggagagtat 4380 ggatgatggc gtgatggcct ggaacgaaca gggcgtattg cagttcctca atgcgcgcgc 4440 ggctctgctt ttgcatctcg acgcgcaggc cagccagggg aagaatatta acgacctgct 4500 caatctcccc atgctgctgc gtcgggcgat caaacatgcc cgcggcctga atcatgtcga 4560 agtcaccttt gaaagccagc accagtttgt tgatgccgtc atcaccctga aaccgattgt 4620 tgaagagcag ggcaacagct ttatcctgct gctgcatccg gtggagcaaa tgcgtcaact 4680 gatgaccagc cagctcggca aggtgagcca cacctttgag cagatgtcga cagacgatcc 4740 agaaacccga cgtttgatcc actttggtcg tcaggcggcg aggggaagtt tcccgatcct 4800 gctgtgcggg gaagagggcg tcggtaaaga gctactgagt caggccattc acaatgaaag 4860 cgaacgcgcc agcgggccgt atatcgccgt gaactgccag ctgtatgcca acagcgtact 4920 ggggcaggat tttatgggca gtgcgcccac tgatgatgaa aacggtcggc tgagccgtct 4980 tgaactggcg aacggcggca cgctgtttct ggaaaaaatt gaatatctgg cgccggagtt 5040 acaatccgcg ctgctgcagg tgatcaagca gggggtttta acacgcctgg atgcccggcg 5100 gctgattccg gtggatgtca aagtgatcgc caccaccacg gttgatctcg ctaatctggt 5160 ggagcaaaac cgctttagcc gtcagttgta ttacgcgctg cactcttttg agatcgtaat 5220 cccgccgctg cgggcgcggc gtaacagtat tccatcgctg atttatacgc ggttgaatag 5280 cctgaaaaaa cgcttttcat ccagcctgaa gatcgacgac gacgcgttgg cacaactggt 5340 ggcctattcg tggccgggta atgattttga gctgaacagc attattgaaa acatcgctat 5400 cagcagtgat aacggccata ttcggctgag caatctgccg gattatctgt ttgccgaacg 5460 ccctggtctg gagacggcat cttcactgct gcccgccagc ctgaccttta ccgccatcga 5520 gaaagaggcg attatccatg ccgcccgcgt gaccagtggg cgtgttcagg agatgtcgca 5580 actgctgaat attggtcgca ccaccctgtg gcgtaagatg aagcagtacg acatcgatgc 5640 cagccagttt aagcgcaaac atctggagta gtttcttcga ttctggccat cgagaacagc 5700 gcatccgaca gccggttgat ataacgcctg gcctcatcgc gtaacgtcag cgttctgccc 5760 atcgcgatga ggatccgctc cagccgacgc gtcagggttc gcgcaacgtg cagctgtgcc 5820 gatgccagat ttttccccgg aataacaaac tctttgagcg ggccgctttg cgccatattg 5880 cggtcaataa gctgctccag cgcctgaata tcctcttcgc caatacgctg ttttaagcga 5940 gtgagccctt tctgatcgct ggcgagttcc gcccccagta cgaacagcat cttctgcata 6000 gcgtgcaatt cctggcgtag ctcggcctgg cgcgtgctgg catagcagac gccaagctgg 6060 gaaatcagtt catccaccgt gccgtaggcc tcaacgcgga tgtcgtcttt atcgatacgg 6120 ctgccgccaa acagcgcggt cgtgccgtta tcaccggttc gggtatagat gcggtacatt 6180 tattttatct cgctgaaggg aagaacttta accagttgac cggcattggc accgagcgtg 6240 cggatctgtg ctgtcccggc gcgggtatgc ccgcagacca gcgcccgatc ctcgggcaac 6300 tgggcatggg ttaaggcaat atcaccggtg gcgttgagac cgagacccac gcgcagcgtc 6360 gaacttttgg ccgccagttt gccgagcgcc agcgcgcagt catgatcgtc gcaacagatt 6420 gcccggcagg gaacgccttg ctcttccagc ccccaacaca gctcatcaag cacgcgagta 6480 ccctgccagc gtgagtgata aaacagatgt acgcccggtg atgaaagtga cattgcgccc 6540 cctgcgttat cagaatgcct gacggaagat ctcggcaatt tctttctcgt tccctttgcg 6600 tgggttggag aaggcgttgc cgtccttcag tgccatttca gccatatacg ggaaatcggc 6660 ttctttgacg cccagatcgc gcagatgctg cggaataccg atgtcggcgg agaggcgggc 6720 aatagcatga atggccagct cggcggcatc catggtggag agtccgtccg tgttctcgcc 6780 cataaactct gcgatgtcgg caaatttttc cgggttagcg atcaggttat agcgcgctac 6840 gtgcggcagc agtacggcat tcgccacgcc gtgcggcatg tcgtaaagac cgccaagctg 6900 atgcgccatc gcgtgaacgt agccgaggtt ggcgttgttg aaggccatac ccgccagcag 6960 ggaggcgtag gccatgttct cgcgagcttt caggttgctg cccagcgcca cggcctggcg 7020 caagttacgg gcgatcaggc ggatcgcctg gatagcggca gcgtcggtga ccgggttggc 7080 atctttggaa atgtaggctt ccacggcgtg ggtcagggcg tccatcccgg tagccgcagt 7140 cagtggggct ggcttgccga gcattagcag cggatcgtta atggagaccg acggcaggtt 7200 gcgccagctg acaatcacaa acttcacttt ggttttggta ttggtcagca cgcagtggcg 7260 ggtgacttcg ctggcggtac cggcggtggt attcaccgca acgatcggcg gcagcgggtt 7320 ggtcagggtt tcaatcccgg catagctgta gagatcccct tcgtgagtcg cggcgatacc 7380 gatgccttta ccgcagtcat gcgggctacc gccgccaacg gtaacgatga tgtcgcaatg 7440 ctctttccga aagacctcca ggccgtcgcg cacgttggtg tctttggggt ttggctcaac 7500 gccgtcaaaa accacgacgt caataccggc ttcacgcaga tgtgtgaggg ttttatctac 7560 cgcgccgtct ttaatcgccc gcagaccttt atcagtgacc agcagcgctt ttttaccgcc 7620 caacagtttg cagcgttcgc cgaccacgga aatagcattg gggccaaaga agttcacatt 7680 tggcaccagg taatcaaaca tacgatagct cataatatac cttcttagat ttaaattata 7740 attcagagaa acaatccaat gcggtttctg ctaataatct gtcttgttcg acggtaccgc 7800 cacttacacc aacggcaccg attactttgc cgtttaatat gacaggtaaa ccgccgccaa 7860 aaataacaat tcgttgttga ttggtgagct gcaagccata tagagaggca cctggttgta 7920 ccgcatcagt aatttcatgg gtaccctgtc gcaggcagca ggcggtataa gctttattaa 7980 gagagatatc acagctggtg acaaacgcgt cgtccatacg ttgcattagc agcgtgttcc 8040 cgccgtgatc caccacggaa aacaccaccg gaacgttgat ttcaagcgct ttagcctcga 8100 cggcctgcgc cattttcttt gcagccgcca gcgtaatggt ggcgatttgt tggctcttat 8160 tcacagtctc tcctgaaaag gttgtagggc tgaggtcacg aaaatatcgc gcaacagcta 8220 tttttatcat tggctttatc ttattttctc gccccgcgtt attaagtcaa tagggtggga 8280 atttcaaaaa tattaccttc agtttcctgt tgtggcgttt attgataatg atcatttaac 8340 ctcgtcacaa ttcacggcga atattcctga gccgcgtttc aatatgaaac gatggttttt 8400 tattacgtgc caaattggaa cgaaattaat tttatttttt tattgtcctg ctcatttaga 8460 gttcggctat tgctgatgaa aggcgaatgc cgggtgttgt gtccccggca atacggcccg 8520 gcgtccctat tttttatctt ttgagtccat aaactatgag aagatcaaaa cgattcgaag 8580 tgcttgccca gcggcctgta aatcaggatg ggctaattgg cgaatggcct gaagaggggc 8640 tgatcgcaat ggaaagccct tacgatccgg cttcgtcggt gaaggtggaa aatggtcgta 8700 ttgttgagct ggacggcaag agccgcgccg agttcgacat gattgaccgg ttcattgccg 8760 attacgcgat taacgtgccc gaggccgagc gcgccatgca gctcgacgcg ctggagatcg 8820 ccagaatgct ggtggatatc cacgtcagtc gggaagagat cattgccatt acgactgcca 8880 tcaccccggc gaaacggctg gaggtgatgg cgcaaatgaa cgtggtggag atgatgatgg 8940 cgctgcaaaa aatgcgcgcc agacgcaccc cctccaatca gtgccacgtg accaacctga 9000 aagataaccc ggtacagatt gctgccgacg ctgctgaagc ggggatccgt ggcttttccg 9060 aacaggaaac aacggtcggg atcgcccgct atgcgccgtt taacgccctg gcgctgctgg 9120 tggggtccca atgcggtgcc cccggcgtgc tgacgcaatg ctccgttgaa gaggccacgg 9180 agctggagct gggaatgcgt gggctaacca gctacgccga aacggtgtcg gtatatggca 9240 cggaatcggt gtttaccgat ggcgatgata cgccgtggtc aaaagcgttt cttgcctcgg 9300 cgtatgcttc tcgtggtctg aagatgcgct acacctccgg caccggttcg gaagcgctga 9360 tgggctattc cgaaagtaaa tcgatgcttt acctggagtc gcgttgcatt tttatcacca 9420 aaggtgcggg cgtacagggg ttacaaaacg gcgcggtcag ctgtatcggc atgactggcg 9480 ctgtgccgtc ggggattcgc gcggtgctgg cggaaaacct gattgcctcg atgctcgacc 9540 ttgaagtggc ctccgccaat gaccagacct tttcccactc cgatattcgt cgtactgccc 9600 gcaccctgat gcagatgctg ccgggcaccg actttatctt ctccggctat agcgcggtac 9660 cgaactacga caacatgttt gccgggtcga acttcgacgc tgaagacttc gatgattaca 9720 acattctgca acgcgatctg atggttgatg ggggcctgcg tccggtcacg gaagaggaga 9780 caatcgccat ccgcaataag gccgcgcgcg ccattcaggc ggtgttccgc gaactgggtc 9840 tgccgctgat tagcgatgaa gaggtggacg ccgccactta cgctcacggc agcaaagata 9900 tgcccgcgcg caatgtggtg gaagatttgg ctgccgtgga agagatgatg aagcgcaata 9960 tcaccgggct ggatatcgtc ggcgcgttga gttccagcgg cttcgaagac atcgccagca 10020 atatcctcaa catgctgagg cagcgtgtta ctggtgatta cctgcaaacg tcggccattc 10080 tggatcgtca gttcgacgtc gtcagcgccg ttaatgacat caacgattat cagggacccg 10140 gcaccggcta tcgcatctct gccgaacgtt gggcggaaat taaaaatatt gcgggcgtgg 10200 ttcagcccgg ctcgatcgaa taaggcggta cacagtggaa tgcacaactg aacgtaagcc 10260 ggttttcacc ttgcaggtga gtgaaggcga ggcggctaaa gcggacgaac gcgtggatga 10320 agtcgtgata ggcgtcggcc cggcctttga taagtatcag cacaaaacat tgattgatat 10380 gccgcacaaa gcgattttga aagaactggt tgccggcatt gaagaagagg ggctgcatgc 10440 acgggtggtg aggattctgc gcacttctga tgtttcattt atggcatggg atgcggcaaa 10500 tctcagcggt tctggcatcg ggattggtat ccagtcgaaa ggaaccacgg tgattcatca 10560 gcgcgatctg ctgcctctga gcaaccttga gctgttctct caggctccgc tgctgacgct 10620 ggaaacctat cgccagattg gtaaaaacgc cgcgcgctat gcacgtaaag agtcgccttc 10680 gccagtgcca gtggttaatg accagatggt acgccccaaa tttatggcca aggccgcgct 10740 gtttcatatc aaagaaacca aacacgtggt gcaggatcgt gcgcctgtca cgctgcatat 10800 tgcacttgta agggaatgac catgaacgac aacatcatga ccgcgcagga ttatccatta 10860 gcaacccgct gccctgagaa aatccagact cctaccggca aaccgctaac cgagattacc 10920 cttgagaatg tgctggcggg ccgtgtaggt ccgcaggatg tgcgtatttc tcagcaaacg 10980 ctggagtatc aggcgcagat tgccgaacag atgcagcgtc acgccgtggc gcgtaatttc 11040 cgtcgtgcgg cggagcttat agcaattccg gatgcccgta ttcttgaaat ttacaacgcg 11100 ctgcggccgt ttcgttcttc gttcgccgag cttcaggcca ttgccgatga actggaacat 11160 acctggcatg ccacggtgaa cgccgggttt gtccgcgagt cggcagaggt gtatctgcag 11220 agaaataagc tgcgtaaagg cagccagtga cggaggttgt atgccgttaa ttgcagggat 11280 tgatatcggc aacgccacca ccgaagtggc gttggcgcag catggccggt ttatcgccag 11340 cgggattgtc gccacgaccg gtatgaaagg cacacgggac aatattgccg gggtggtggc 11400 ctccctgcag caggccctga ataacacccc gtggtcgctt caggatgtgg cgaaaatctg 11460 catcaacgag gcggcaccgg tgattggcga tgtggcgatg gaaaccatca ctgaaaccat 11520 cattaccgaa tcaacaatga ttgggcataa cccgcaaacg cccggcggcg ttggcgtggg 11580 aatgggaacc accatcgccg tggagaagct ggcggcgttg agtgaagatc ggtttaccca 11640 gggctggatc ccgctggtcg gtgaggagag ggatttcctc gaggcggttt ggtttatcaa 11700 tgaggcgctg gatcgcggtg tcaacgtggt ggcggcgatc ctgaaaaaag atgatggcgt 11760 gctggtgaac aatcgcctgc gtcgaacaat gccggtggta gatgaagtca ccctgctgga 11820 gaaggtgccg gaaggtgtgc tggcggcggt ggaagtggca gccccaggac aggtggtgcg 11880 tgtgctgtcg aatccttacg gcatcgcgac cttttttacc ctgacgcccg aggaaacgca 11940 aactatcgtc cccatcgcca gagcgctaat tggcaaccgt tccgccgtgg tgctgaaaac 12000 cccgcagggt gatgtgcgct caagggtgat cccggcgggt aagatcttta tccgcggcga 12060 aaagcgtggc ggtgaagccg acgtagcgca aggcgcacag gccattatgc aggcgatgag 12120 cacctgtgcg cccgtgtgcg atattcgtgg tgaagcaggt acgcatgcag gtggcatgct 12180 ggagcgggtg cgtaaggtga tggcctcact gaccggtcac gacatgaacg cggtgtatat 12240 tcaggatctg ttggcggtgg atacgtttat tccccgcaag gtgcagggcg gtattgccgg 12300 ggagtgttca atggagaacg ccgttggcat tgcggcgatg gtgaaatccg atcgcttaca 12360 gatgcaggcc atcgccagtg agctgagcgc ccggttaaac atcccggttg aagtcggcgg 12420 cgtggaggcc aacatggctg tcgccggtgc gttaacaacg cccggatgtg ctgcaccgct 12480 ggcgatcctc gatttaggcg caggctctac cgatgccgcc attatcaata gcgatggcgt 12540 tgtgaaagcg gtccatctgg ctggcgcggg gaatatggtc agcctgttga ttcaaaccga 12600 gctgggctta agcgatccgt ttctggcgga ggaaattaaa aaatacccgc tggcaaaagt 12660 ggagagcctg ttcagcattc gccacgaaaa cggcgcggtg gagttttttc gcgaaccgct 12720 cagtccgtca gtgtttgcca aagtcgttta tctgaaaaac ggcgagctga tcccagtaga 12780 taaccagacc tcgctggaaa aaattcggtt ggtgcgccga caggccaaag agaaggtttt 12840 tgtgaccaac tgcctgcgcg ctttacgcca ggtatcaccc gggggctcta ttcgtgatat 12900 gacttttgtg gtgctggtgg gcggctcctc gctggatttc gaaatcccgc agatgatcac 12960 cgacgcgctg gcccattacg gcgtggttgc cggacagggc aacatccgtg gtaccgaagg 13020 gccgcgtaat gcggtagcga ccgggctggt tttggccggg atagcgaatt aatcgtgcag 13080 aaaacagccg ccgcaagcgt ttagctgcgg ggtaaatcaa ataaaggt 13128 // |
|