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EBI DbfetchID U09581; SV 1; linear; genomic DNA; STD; PRO; 3665 BP. XX AC U09581; XX DT 20-MAY-1995 (Rel. 43, Created) DT 17-APR-2005 (Rel. 83, Last updated, Version 2) XX DE Nocardia aerocolonigenes NaeI restriction endonuclease (naeIR), NaeI DE modification methyltransferase (naeIM), and hypothetical NaeI DE very-short-patch-repair endonuclease (naeIV) genes, complete cds. XX KW . XX OS Lechevalieria aerocolonigenes OC Bacteria; Actinobacteria; Actinobacteridae; Actinomycetales; OC Pseudonocardineae; Actinosynnemataceae; Lechevalieria. XX RN [1] RP 1-3660 RX DOI; 10.1016/0378-1119(94)00806-4 RX PUBMED; 7698663. RA Taron C.H., Van Cott E.M., Wilson G.G., Moran L.S., Slatko B.E., RA Hornstra L.J., Benner J.S., Kucera R.B., Guthrie E.P.; RT "Cloning and expression of the NaeI restriction endonuclease-encoding gene RT and sequence analysis of the NaeI restriction-modification system"; RL Gene 155(1):19-25(1995). XX RN [2] RP 1-3665 RA Guthrie E.P.; RT ; RL Submitted (11-MAY-1994) to the EMBL/GenBank/DDBJ databases. RL Ellen P. Guthrie, Research, New England Biolabs Inc., 32 Tozer Road, RL Beverly, MA 01915, USA XX CC NCBI gi: 775101 XX FH Key Location/Qualifiers FH FT source 1..3665 FT /organism="Lechevalieria aerocolonigenes" FT /strain="ATCC 23870" FT /mol_type="genomic DNA" FT /db_xref="taxon:68170" FT CDS 146..1099 FT /codon_start=1 FT /transl_table=11 FT /gene="naeIR" FT /standard_name="R.NaeI" FT /product="NaeI restriction endonuclease" FT /function="cleaves dsDNA at GCCGGC, forming GCC'GGC" FT /note="NCBI gi: 775102" FT /db_xref="GOA:P50187" FT /db_xref="InterPro:IPR011991" FT /db_xref="PDB:1EV7" FT /db_xref="UniProtKB/Swiss-Prot:P50187" FT /experiment="experimental evidence, no additional details FT recorded" FT /protein_id="AAC43324.1" FT /translation="MTELPLQFAEPDDDLERVRATLYSLDPDGDRTAGVLRDTLDQLYD FT GQRTGRWNFDQLHKTEKTHMGTLVEINLHREFQFGDGFETDYEIAGVQVDCKFSMSQGA FT WMLPPESIGHICLVIWASDQQCAWTAGLVKVIPQFLGTANRDLKRRLTPEGRAQVVKLW FT PDHGKLQENLLLHIPGDVRDQIFSAKSSRGNQHGQARVNELFRRVHGRLIGRAVIATVA FT QQDDFMKRVRGSGGARSILRPEGIIILGHQDNDPKVANDLGLPVPRKGQVVAARVVPAD FT EGDQRQTAEIQGRRWAVAVPGDPIVEAPVVPRKSAE" FT CDS complement(1129..1629) FT /codon_start=1 FT /transl_table=11 FT /gene="naeIV" FT /standard_name="V.NaeI" FT /note="hypothetical NaeI very-short-patch-repair FT endonuclease, tentative identity based on similarity to the FT E. coli Vsr protein, Swiss-Prot Accession Number P09184; FT NCBI gi: 775104" FT /db_xref="GOA:P50186" FT /db_xref="HSSP:1CW0" FT /db_xref="InterPro:IPR004603" FT /db_xref="UniProtKB/Swiss-Prot:P50186" FT /protein_id="AAC43326.1" FT /translation="MSDKSSRAAARARAHASGTYPAPLNAGRSRNMQANRRSGTKPEAA FT LRSALFKLGYRYRKDFLLRLGDGVKVKPDIVFTARKVAVFIDGCFWHVCPDHGRQPTTN FT EWYWSPKLRRNVERDRTVNQSLTNAGWRVLRVWEHEELQDAVAAVVDTLHHLEHGFDTS FT AED" FT CDS 1734..2975 FT /codon_start=1 FT /transl_table=11 FT /gene="naeIM" FT /standard_name="M.NaeI" FT /product="NaeI modification methyltransferase" FT /function="methylates dsDNA at GCCGGC" FT /note="GTG start codon; NCBI gi: 775103" FT /db_xref="GOA:P50188" FT /db_xref="InterPro:IPR018117" FT /db_xref="UniProtKB/Swiss-Prot:P50188" FT /experiment="experimental evidence, no additional details FT recorded" FT /protein_id="AAC43325.1" FT /translation="MQSLEVVEICAGAGGQALGLEKAGFSHRLAVELDVNAAATLRKNL FT KSDVVITGDVADPSVLNPMEHLGVSLLAGGVPCPPFSIAGKQLGADDMRDLFAWAVELC FT DVMKPRALMLENVRGLSMPRFAGYRQHVLDRLNDMGYVAEWRLLHASDFGVPQLRPRFV FT LVALQNKFAPYFTWPEPTGAAPTVGETLKDLMAADGWEGAEEWAAQANDIAPTIVGGSK FT KHGGADLGPTRAKRAWAELGVDAMGVADAPPQPGDKFKVGPKLTCEMVARIQGWRDGEW FT IFEGRKTSRYRQIGNAFPPPVAEAIGKRIRAALNMEGEGRDRAVDSDHNPLYRALKESG FT DFMTHRQLERAVGRPIEAYELERTISDLGRDFEVETKDGASAMAYKLGPFKAFTGQEGH FT LRHEMFVRHRTKIS" XX SQ Sequence 3665 BP; 695 A; 1066 C; 1204 G; 700 T; 0 other; ggtaccggca tcaccatggg ccggagtttc tcgctcatgg cctgcgcgga caacttcaca 60 cccatgcctt gagtgcttcg agcacagaat ggagcgtgtc attgggggca gcccatgcca 120 agatcatcag atttgaaggg gtcacatgac tgagttgccg ctgcagttcg cggaacccga 180 tgacgatctc gagcgggttc gggcaacgtt gtacagcctt gacccagacg gtgaccggac 240 tgctggtgtg ttgagagaca cgctcgacca gttgtacgac ggtcagcgaa ccgggaggtg 300 gaacttcgat cagctgcaca agaccgagaa gacgcacatg ggaaccctgg tggagatcaa 360 cctgcaccgt gagttccagt tcggtgacgg ctttgagacc gattacgaga ttgcaggagt 420 gcaggtcgac tgcaagtttt cgatgagcca gggcgcttgg atgctgcctc cggagtcgat 480 cgggcacatc tgtctggtca tctgggcaag tgatcagcag tgcgcatgga ccgcaggact 540 ggtgaaggtc ataccccagt tcctcggcac tgccaaccgt gacctcaagc ggcgactcac 600 acccgaaggc cgtgcccaag ttgtcaaact gtggccagat cacggaaagc tgcaggagaa 660 cctgctcctg cacatccccg gtgacgtgcg cgatcagatc ttctcagcga aatccagccg 720 cggtaatcag cacggtcagg cgcgcgtgaa cgaactgttc cgccgagtgc acgggcgtct 780 catcgggaga gcggtcatag cgactgtggc gcagcaggac gacttcatga agcgcgtacg 840 cgggtcaggc ggcgcgcgtt cgatccttcg gcctgaagga atcatcattc ttgggcatca 900 ggacaacgat ccgaaggtgg cgaacgatct cgggttgccg gtgccgcgca aggggcaggt 960 cgtcgcagca cgagtggtac cggctgacga gggagaccag cggcaaaccg ctgagatcca 1020 ggggcggcgc tgggccgtag ccgtgcctgg cgaccccatc gtcgaggcgc cggttgtgcc 1080 ccggaaatca gccgagtagg gcgtggcgcg tccagactcc gggaattgtc agtcctccgc 1140 actagtgtcg aacccatgtt cgagatgatg aagggtgtcc acgacggctg ctaccgcgtc 1200 ttgcagctct tcgtgctccc aaacccgcaa caccctccac ccggcgttgg tgagggactg 1260 gttgaccgtt cgatcgcgct cgacgttccg cctgagcttc ggcgaccagt accactcgtt 1320 cgtcgtaggc tgtcgtccgt ggtctgggca cacatgccag aagcagccgt cgatgaagac 1380 agcgaccttc cgggcggtga agacgatgtc gggtttgacc ttgacgccgt cgccaaggcg 1440 cagcaggaaa tccttgcgat acctgtaccc gagcttgaag agcgcgcttc gcagtgcagc 1500 ctcgggtttc gtaccactgc gccggttcgc ctgcatgttc cgcgagcgcc cagcgttcag 1560 cggcgctggg taggtaccgc tcgcgtgtgc tcgagcgcgg gcggcagctc tgctgctctt 1620 atcagacatg cgcgaaagcc tcttgtgccg gttggctaca ggtacggggc gccggtggac 1680 gatactgcat cgcgaggtac acctgatcac atttggacgc gaaaggggcg cttgtgcaga 1740 gtctcgaggt agtggagatc tgcgccggtg ccggtggtca ggcgctgggg cttgagaaag 1800 ctggcttcag tcatcggctt gccgttgagc tggacgtgaa cgcggcagcg acgctgcgca 1860 agaacctcaa gtcggacgtg gtgatcactg gcgacgtcgc tgatccttcc gtgctgaacc 1920 cgatggaaca cctgggggtg tcgttgctgg ctggtggtgt gccttgtccc ccattcagca 1980 tcgcgggcaa gcagctcggt gccgacgaca tgcgggacct gttcgcctgg gcggttgagc 2040 tgtgcgatgt catgaagccg cgcgccttga tgctcgagaa cgtccgtggc ctcagtatgc 2100 ccaggttcgc cggctaccgg cagcacgtcc tcgatcggct gaacgacatg ggttacgtcg 2160 ctgagtggcg tctcctgcac gcatcggact ttggggttcc tcaactccgg ccgcgtttcg 2220 tacttgtcgc tctgcagaac aagttcgccc cctatttcac ctggcctgag ccgaccggtg 2280 cggcacccac ggtgggggag acgttgaagg acctcatggc cgcggacggc tgggaaggtg 2340 ccgaagagtg ggcggctcag gcgaacgaca tcgcaccaac catcgtgggt ggctccaaga 2400 aacatggcgg agctgacctc ggcccgactc gcgcgaagcg ggcgtgggca gagctcggtg 2460 tcgacgcaat gggagtcgct gacgcgccgc cccagcctgg cgacaagttc aaggtaggac 2520 cgaagctgac ctgcgagatg gttgccagga tccaagggtg gcgcgacggc gagtggatct 2580 tcgagggtcg taagacctcg cgataccgcc agatcggtaa cgctttcccg ccacccgtgg 2640 ctgaagcgat cggcaagcgc atccgtgctg ccttgaacat ggagggtgag ggcagggatc 2700 gggcggtcga cagcgaccac aacccgttgt accgggcgct gaaggagtcg ggcgatttca 2760 tgactcaccg gcagctggaa agggctgtcg gtcgacccat cgaggcatat gagctggagc 2820 gcacgatctc tgatctgggg cgtgacttcg aggtcgagac gaaggacggt gcttcggcga 2880 tggcgtacaa actggggccg ttcaaggcct tcacaggcca agagggtcat ttgcggcacg 2940 agatgttcgt gcgccaccgc acaaagatca gctaggagga aggctggatg tccacatagg 3000 caaagtgccc ggctaaggtg gacatccggc tcagcatcag tcgtcgtccc cgacgatggc 3060 gatgaggtct tttttcgaca tcgcgacgta cttcttcctc ttcgcggtgt tccggatgat 3120 cgcagggatc agctcgtagg tcagatcgag tgatccgagc acgtagtagt cgatgtcgtt 3180 tgactcgagg ctcacaatat ccatgcccag ttcgcgcaac tctcgcaggc ggcgctcggt 3240 gtggacggag tcggcggtca cgaccctgag cagggctgcc tccacgttct ggcctttgcg 3300 ttgcaaaagc aggctgaaca actcctcgtg cacacggctc ccatagggaa cggcgagata 3360 ggccgcagga acggtgccac cgtaccgtcg ctccatttca gtgccgagtt gggcgcgcag 3420 ctcatgaaac tcttcgcacc gctccggaag gcccagccgt acgtaagcct gcatggactc 3480 agcgaggagc cgcttagcgt acaaataggt gtcgagcagg tcctgactgg aggttccgct 3540 ccgaagctgg accagcagat cttcggttgc ggccgctgta tcgggcctca accggctcca 3600 gtcaccctgc ggataccgag atgcggtcaa gctatttccc cttctgcttc agctggttac 3660 tgcag 3665 // |
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