EBI Dbfetch

ID   U08465; SV 1; linear; genomic DNA; STD; PRO; 1937 BP.
XX
AC   U08465;
XX
DT   01-MAY-1994 (Rel. 39, Created)
DT   17-APR-2005 (Rel. 83, Last updated, Version 6)
XX
DE   Clostridium acetobutylicum ATCC 824 acetyl coenzyme A acetyltransferase
DE   gene, complete cds, and homolog of B. subtilis orfX gene, partial cds.
XX
KW   .
XX
OS   Clostridium acetobutylicum
OC   Bacteria; Firmicutes; Clostridia; Clostridiales; Clostridiaceae;
OC   Clostridium.
XX
RN   [1]
RP   1-1937
RX   DOI; 10.1016/0378-1119(94)00838-J
RX   PUBMED; 7867955.
RA   Stim-Herndon K.P., Petersen D.J., Bennett G.N.;
RT   "Characterization of an acetyl-CoA C-acetyltransferase (thiolase) gene from
RT   Clostridium acetobutylicum ATCC 824";
RL   Gene 154(1):81-85(1995).
XX
RN   [2]
RP   1-1937
RA   Stim K.P.;
RT   ;
RL   Submitted (08-APR-1994) to the EMBL/GenBank/DDBJ databases.
RL   Kathleen P. Stim, Dept. of Biochemistry and Cell Biology, Rice University,
RL   Houston, TX 77251, USA
XX
FH   Key             Location/Qualifiers
FH
FT   source          1..1937
FT                   /organism="Clostridium acetobutylicum"
FT                   /strain="ATCC 824"
FT                   /mol_type="genomic DNA"
FT                   /clone="pTeco11"
FT                   /db_xref="taxon:1488"
FT   CDS             <1..166
FT                   /codon_start=2
FT                   /transl_table=11
FT                   /note="C-terminus of open reading frame similar to Bacillus
FT                   subtilis orfX, GenBank Accession Number Z22516"
FT                   /db_xref="GOA:P45360"
FT                   /db_xref="HSSP:1F6D"
FT                   /db_xref="InterPro:IPR003331"
FT                   /db_xref="UniProtKB/Swiss-Prot:P45360"
FT                   /protein_id="AAA82723.1"
FT                   /translation="DEAYKIMKDEEEYEKMSKAINPYGDGKASDRIVDAILYHFGVLKD
FT                   RPDEFSPKK"
FT   -35_signal      356..361
FT   -10_signal      379..384
FT   misc_signal     388
FT                   /function="transcription start site"
FT   RBS             478..483
FT   CDS             493..1671
FT                   /codon_start=1
FT                   /transl_table=11
FT                   /product="acetyl coenzyme A acetyltransferase (thiolase)"
FT                   /db_xref="GOA:P45359"
FT                   /db_xref="HSSP:1M3K"
FT                   /db_xref="InterPro:IPR016038"
FT                   /db_xref="UniProtKB/Swiss-Prot:P45359"
FT                   /protein_id="AAA82724.1"
FT                   /translation="MKEVVIASAVRTAIGSYGKSLKDVPAVDLGATAIKEAVKKAGIKP
FT                   EDVNEVILGNVLQAGLGQNPARQASFKAGLPVEIPAMTINKVCGSGLRTVSLAAQIIKA
FT                   GDADVIIAGGMENMSRAPYLANNARWGYRMGNAKFVDEMITDGLWDAFNDYHMGITAEN
FT                   IAERWNISREEQDEFALASQKKAEEAIKSGQFKDEIVPVVIKGRKGETVVDTDEHPRFG
FT                   STIEGLAKLKPAFKKDGTVTAGNASGLNDCAAVLVIMSAEKAKELGVKPLAKIVSYGSA
FT                   GVDPAIMGYGPFYATKAAIEKAGWTVDELDLIESNEAFAAQSLAVAKDLKFDMNKVNVN
FT                   GGAIALGHPIGASGARILVTLVHAMQKRDAKKGLATLCIGGGQGTAILLEKC"
FT   stem_loop       1683..1729
XX
SQ   Sequence 1937 BP; 757 A; 222 C; 395 G; 563 T; 0 other;
     agacgaagcc tataaaataa tgaaagatga agaagaatat gaaaaaatga gtaaggcaat        60
     aaatccatat ggtgatggaa aggcttcaga tagaatagtt gatgctatat tgtatcattt       120
     tggagtatta aaggatagac cagatgaatt ttcacctaaa aagtaataca tgagcattct       180
     aaaagaattt agaatgaagt ttcttatgca caagtatttt ttattacatt aatatagtta       240
     aaatataaac ttatgtattt atgctaaaac atgattttaa gggggttagc atatgcataa       300
     gtttaatttt tttgttaaaa aatattaaac tttgtgtttt ttttaacaaa atatattgat       360
     aaaaataata atagtgggta taattaagtt gttagagaaa acgtataaat tagggataaa       420
     ctatggaact tatgaaatag attgaaatgg tttatctgtt accccgtatc aaaatttagg       480
     aggttagtta gaatgaaaga agttgtaata gctagtgcag taagaacagc gattggatct       540
     tatggaaagt ctcttaagga tgtaccagca gtagatttag gagctacagc tataaaggaa       600
     gcagttaaaa aagcaggaat aaaaccagag gatgttaatg aagtcatttt aggaaatgtt       660
     cttcaagcag gtttaggaca gaatccagca agacaggcat cttttaaagc aggattacca       720
     gttgaaattc cagctatgac tattaataag gtttgtggtt caggacttag aacagttagc       780
     ttagcagcac aaattataaa agcaggagat gctgacgtaa taatagcagg tggtatggaa       840
     aatatgtcta gagctcctta cttagcgaat aacgctagat ggggatatag aatgggaaac       900
     gctaaatttg ttgatgaaat gatcactgac ggattgtggg atgcatttaa tgattaccac       960
     atgggaataa cagcagaaaa catagctgag agatggaaca tttcaagaga agaacaagat      1020
     gagtttgctc ttgcatcaca aaaaaaagct gaagaagcta taaaatcagg tcaatttaaa      1080
     gatgaaatag ttcctgtagt aattaaaggc agaaagggag aaactgtagt tgatacagat      1140
     gagcacccta gatttggatc aactatagaa ggacttgcaa aattaaaacc tgccttcaaa      1200
     aaagatggaa cagttacagc tggtaatgca tcaggattaa atgactgtgc agcagtactt      1260
     gtaatcatga gtgcagaaaa agctaaagag cttggagtaa aaccacttgc taagatagtt      1320
     tcttatggtt cagcaggagt tgacccagca ataatgggat atggaccttt ctatgcaaca      1380
     aaagcagcta ttgaaaaagc aggttggaca gttgatgaat tagatttaat agaatcaaat      1440
     gaagcttttg cagctcaaag tttagcagta gcaaaagatt taaaatttga tatgaataaa      1500
     gtaaatgtaa atggaggagc tattgccctt ggtcatccaa ttggagcatc aggtgcaaga      1560
     atactcgtta ctcttgtaca cgcaatgcaa aaaagagatg caaaaaaagg cttagcaact      1620
     ttatgtatag gtggcggaca aggaacagca atattgctag aaaagtgcta gaaagtattg      1680
     ttaaaaataa ctctgtagaa ttataaatta gttctacaga gttatttttt aaaaaaattc      1740
     taaacttatg tataaaaaat acgataagaa tgtagaatta aaactaaaga cagttcaatt      1800
     tcttttagaa taatttagtt agtgtggtaa aaaaatgtca taatgatatt tatgttgaaa      1860
     tttgtataaa attcagaaaa tgaatatatt ttatcaattt tcagtcattt gaaagattat      1920
     gaggctaatg cagtact                                                     1937
//