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EBIDatabasesDatabase BrowsingDbfetch

EBI Dbfetch

ID   U08381; SV 1; linear; genomic DNA; STD; PRO; 1294 BP.
XX
AC   U08381;
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DT   25-MAY-1994 (Rel. 39, Created)
DT   27-OCT-2001 (Rel. 69, Last updated, Version 6)
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DE   Rhodococcus sp. M4 L-phenylalanine dehydrogenase precursor (pdh) gene,
DE   complete cds.
XX
KW   .
XX
OS   Rhodococcus sp.
OC   Bacteria; Actinobacteria; Actinobacteridae; Actinomycetales;
OC   Corynebacterineae; Nocardiaceae; Rhodococcus.
XX
RN   [1]
RP   1-1294
RX   PUBMED; 8206922.
RA   Brunhuber N.M., Banerjee A., Jacobs W.R.Jr., Blanchard J.S.;
RT   "Cloning, sequencing, and expression of Rhodococcus L-phenylalanine
RT   dehydrogenase. Sequence comparisons to amino-acid dehydrogenases";
RL   J. Biol. Chem. 269(23):16203-16211(1994).
XX
RN   [2]
RA   Brunhuber N.M.W., Hummel W., Blanchard J.S.;
RT   "Purification, characterization, and kinetic mechanism of L-phenylalanine
RT   dehydrogenase";
RL   Unpublished.
XX
RN   [3]
RP   1-1294
RA   Brunhuber N.M.W.;
RT   ;
RL   Submitted (06-APR-1994) to the EMBL/GenBank/DDBJ databases.
RL   Norbert M. W. Brunhuber, Albert Einstein College of Medicine, Biochemistry,
RL   1300 Morris Park Ave., Bronx, NY 10461, USA
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FH   Key             Location/Qualifiers
FH
FT   source          1..1294
FT                   /organism="Rhodococcus sp."
FT                   /strain="M4"
FT                   /mol_type="genomic DNA"
FT                   /clone_lib="library pYUB18-RhM4"
FT                   /clone="pKSII-1B"
FT                   /db_xref="taxon:1831"
FT   RBS             117..122
FT                   /gene="pdh"
FT   CDS             129..1199
FT                   /codon_start=1
FT                   /transl_table=11
FT                   /gene="pdh"
FT                   /product="L-phenylalanine dehydrogenase precursor"
FT                   /function="Oxidative deamination of L-phenylalanine with
FT                   reduction of NAD"
FT                   /EC_number="1.4.1.20"
FT                   /note="L-phenylalanine:NAD oxidoreductase, deaminating"
FT                   /db_xref="GOA:Q59771"
FT                   /db_xref="InterPro:IPR016211"
FT                   /db_xref="PDB:1BW9"
FT                   /db_xref="UniProtKB/TrEMBL:Q59771"
FT                   /citation=[2]
FT                   /citation=[1]
FT                   /experiment="experimental evidence, no additional details
FT                   recorded"
FT                   /protein_id="AAA21461.1"
FT                   /translation="MSIDSALNWDGEMTVTRFDRETGAHFVIRLDSTQLGPAAGGTRAA
FT                   QYSQLADALTDAGKLAGAMTLKMAVSNLPMGGGKSVIALPAPRHSIDPSTWARILRIHA
FT                   ENIDKLSGNYWTGPDVNTNSADMDTLNDTTEFVFGRSLERGGAGSSAFTTAVGVFEAMK
FT                   ATVAHRGLGSLDGLTVLVQGLGAVGGSLASLAAEAGAQLLVADTDTERVAHAVALGHTA
FT                   VALEDVLSTPCDVFAPCAMGGVITTEVARTLDCSVVAGAANNVIADEAASDILHARGIL
FT                   YAPDFVANAGGAIHLVGREVLGWSESVVHERAVAIGDTLNQVFEISDNDGVTPDEAART
FT                   LAGRRAREASTTTATA"
FT   mat_peptide     132..1196
FT                   /gene="pdh"
FT                   /product="L-phenylalanine dehydrogenase"
FT                   /note="initiator methionine is removed posttranslationally"
XX
SQ   Sequence 1294 BP; 243 A; 426 C; 402 G; 223 T; 0 other;
     ctgcagagac gttttcgcca aagactggtc acactatgaa gacgcaccct ccgtcgccgc        60
     ggacccgaat cgggactgct cggagttgtc tgcgtccact gcatccatcg aacatcaagg       120
     ggtacatcat gagtatcgac agcgcactga actgggacgg ggaaatgacg gtcacccgat       180
     tcgaccggga gactggtgcc catttcgtca ttcgactcga ttcgacccaa ctcggaccgg       240
     cggccggagg caccagagcc gcacagtact cacagctggc ggacgccctc accgacgccg       300
     gcaaattggc gggggcgatg acgttgaaga tggcagtgag caaccttccg atgggcgggg       360
     gcaaatccgt cattgcgctt cctgcgccgc gtcattcgat cgatccgagc acgtgggcac       420
     gcatcctccg aatccacgcc gagaacatcg acaagttgtc cggcaactac tggaccggac       480
     cggacgtcaa caccaattcg gcagacatgg atactctgaa cgacaccacc gagttcgtgt       540
     tcggacggtc gctcgaacgc ggcggcgcgg gttcgagcgc gttcaccacc gccgttggcg       600
     tgttcgaggc gatgaaggcg accgtcgcgc accgtgggct gggctcactc gacggtttga       660
     cggtcctggt ccaaggactg ggggcagtcg gaggatcatt ggcatccctg gccgccgaag       720
     cgggtgcgca actcctggtg gcagacaccg acaccgagcg agtagcgcac gctgttgcgt       780
     tgggccacac agcggttgcc ctcgaggacg ttctgtccac cccgtgtgat gtcttcgcac       840
     cctgcgcaat gggcggcgtc atcaccaccg aggtggcgcg aacactcgac tgttccgtcg       900
     tggccggtgc cgccaacaac gtcatcgccg acgaggccgc ctcggacatc ctgcacgcac       960
     gcggaattct gtacgctccc gacttcgtgg ccaacgccgg cggtgccatc cacctcgtag      1020
     gccgggaggt tctcggttgg tccgagtcgg ttgtccacga acgagcagtt gccataggcg      1080
     acaccctgaa tcaggtcttc gagatctccg acaacgacgg cgtcaccccg gacgaggccg      1140
     cccgcactct cgctggacgg cgcgcccgcg aggcctcgac aacgacagcg actgcctagt      1200
     aatcgatctc ggagtctggc gatcgaccat cggtccccat ctggcacgga cggtcatgcg      1260
     agggtcggcg tcccgtccag tccgcctcgg tcct                                  1294
//
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