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EBI DbfetchID U07818; SV 1; linear; unassigned DNA; STD; PRO; 5026 BP. XX AC U07818; S66216; XX DT 31-MAR-1994 (Rel. 39, Created) DT 17-APR-2005 (Rel. 83, Last updated, Version 4) XX DE Bacillus stearothermophilus XL-65-6 PTS regulatory protein (celR') gene, DE partial cds, and cellobiose phosphotransferase system operon (celA, celB, DE celC, and celD) genes, complete cds. XX KW Cellobiose PTS operon. XX OS Geobacillus stearothermophilus OC Bacteria; Firmicutes; Bacillales; Bacillaceae; Geobacillus. XX RN [1] RP 1-5026 RX PUBMED; 8407820. RA Lai X., Ingram L.O.; RT "Cloning and sequencing of a cellobiose phosphotransferase system operon RT from Bacillus stearothermophilus XL-65-6 and functional expression in RT Escherichia coli"; RL J. Bacteriol. 175(20):6441-6450(1993). XX RN [2] RP 1-5026 RA Lai X., Ingram L.O.; RT ; RL Submitted (17-MAR-1994) to the EMBL/GenBank/DDBJ databases. RL Xiaokuang Lai and Lonnie O. Ingram, Dept. of Microbiology and Cell Science, RL University of Florida, 3103 McCarty Hall, Gainesville, FL 32611-0330, USA XX CC On Mar 30, 1994 this sequence version replaced gi:433021. XX FH Key Location/Qualifiers FH FT source 1..5026 FT /organism="Geobacillus stearothermophilus" FT /strain="XL-65-6" FT /mol_type="unassigned DNA" FT /clone_lib="genomic" FT /note="isolated from rotting wood" FT /db_xref="taxon:1422" FT CDS <1..1393 FT /codon_start=2 FT /transl_table=11 FT /gene="celR'" FT /product="regulatory protein" FT /db_xref="GOA:Q45398" FT /db_xref="InterPro:IPR011608" FT /db_xref="UniProtKB/TrEMBL:Q45398" FT /protein_id="AAA17388.1" FT /translation="PDVLELTEAMVVYAEKKLGRRLAEKVMYALAMHIQTAINRLRAGT FT IVSHPKLNEVRAAYKQEFAVALDCLQLMEERTNIDFPIDEAGFLTMFFAFHEEQAEERE FT ERVAIVVVMHGNGVASAMADVVNQLLAAACVHAVDMPLDADPKRIYEQVKAVLQPVASK FT KGALLLVDMGSLVSFSNFLEKELAVPVRVISAASTPHVLEAARKAMLGYALQEIYEEVK FT AAAPFYIRGPLWEEEAAEQDMLAIVTACFTGKGSALALKHILETYLQLDERMWRIIPIQ FT MADAEEARQTLSNVAKHFRIVCIVSHLCLDERIPHFSLEDVLSLRAMKEIQALADVEEI FT HMHMARELTNHLRHLAPARAIPAIRAALAAIGRELGLEADGRDLVGLVFHLCCLLDRLL FT SGERTSGDRGAAKACGHEDEDGALYRAVKEGLFPLEQQYGVCIDEDELCHIVHFFRSLQ FT GKRDG" FT promoter 1482..1513 FT /note="putative" FT RBS 1524..1531 FT /gene="celA" FT /note="putative" FT CDS 1541..1843 FT /codon_start=1 FT /transl_table=11 FT /gene="celA" FT /product="cellobiose phosphotransferase enzyme II'" FT /note="cellobiose PTS enzyme II'" FT /db_xref="GOA:Q45399" FT /db_xref="HSSP:1IIB" FT /db_xref="InterPro:IPR003501" FT /db_xref="UniProtKB/Swiss-Prot:Q45399" FT /protein_id="AAA17389.1" FT /translation="MNILLICAAGMSTSLLVTKMKEAAKQKGIEANIWAVSADEAKSHL FT DQADVVLIGPQIRYKLAAFKKEGEARGIPVDVINPADYGRVNGAGVLDFALRLKK" FT RBS 1842..1851 FT /gene="celB" FT /note="putative" FT CDS 1861..3216 FT /codon_start=1 FT /transl_table=11 FT /gene="celB" FT /product="cellobiose phosphotransferase enzyme II''" FT /note="cellobiose PTS enzyme II''" FT /db_xref="GOA:Q45400" FT /db_xref="InterPro:IPR004501" FT /db_xref="UniProtKB/Swiss-Prot:Q45400" FT /protein_id="AAA17390.1" FT /translation="MDRFIRMLEDRVMPVAGKIAEQRHLQAIRDGIILSMPLLIIGSLF FT LIVGFLPIPGYNEWMAKWFGEHWLDKLLYPVGATFDIMALVVSFGVAYRLAEKYKVDAL FT SAGAISLAAFLLATPYQVPFTPEGAKETIMVSGGIPVQWVGSKGLFVAMILAIVSTEIY FT RKIIQKNIVIKLPDGVPPAVARSFVALIPGAAVLVVVWVARLILEMTPFESFHNIVSVL FT LNKPLSVLGGSVFGAIVAVLLVQLLWSTGLHGAAIVGGVMGPIWLSLMDENRMVFQQNP FT NAELPNVITQQFFDLWIYIGGSGATLALALTMMFRARSRQLKSLGRLAIAPGIFNINEP FT ITFGMPIVMNPLLIIPFILVPVVLVVVSYAAMATGLVAKPSGVAVPWTTPIVISGYLAT FT GGKISGSILQIVNFFIAFAIYYPFFSIWDKQKAAEEQADPTISSGAGATHSL" FT RBS 3215..3221 FT /gene="celC" FT /note="putative" FT CDS 3231..3968 FT /codon_start=1 FT /transl_table=11 FT /gene="celC" FT /note="putative phospho-beta-glucosidase" FT /db_xref="InterPro:IPR006879" FT /db_xref="UniProtKB/Swiss-Prot:Q45401" FT /protein_id="AAA17391.1" FT /translation="MPRYCIVNADDFGYSKGVNYGILEAFQNGVVTSATLMANMPAAEH FT AARLAKDHPELGVGIHFVLTCGRPLADVPSLVNENGEFPRRGEALVGARRGDIERELCA FT QLERFFSFGLTPTHIDSHHHVHEHPNVFPVVEQLAERYRLPIRPVRTARPHRLPTVDVF FT FPDFYGDGLTKDRFISLIDRIGDGQTAEVMCHPAYIDVPLASGSSYCQQRVEELAVLTD FT PTLVAEMAERGVQLITYREFYKL" FT RBS 3967..3975 FT /gene="celD" FT /note="putative" FT CDS 3980..4306 FT /codon_start=1 FT /transl_table=11 FT /gene="celD" FT /product="cellobiose phosphotransferase enzyme III" FT /note="cellobiose PTS enzyme III" FT /db_xref="GOA:Q45402" FT /db_xref="HSSP:2E2A" FT /db_xref="InterPro:IPR003188" FT /db_xref="UniProtKB/Swiss-Prot:Q45402" FT /protein_id="AAA17392.1" FT /translation="MQTYEQTVFQLILHGGNGRSYAMEAITAAKKGEFAEARRLLEQAG FT AELQAAHGLQTALLQQEASGGQPVVTLLMVHAQDHLMTAITVKDLAAEFVELYEALKRQ FT TTES" XX SQ Sequence 5026 BP; 1073 A; 1234 C; 1522 G; 1197 T; 0 other; tcccgacgtc cttgagttga ccgaagcgat ggtcgtctat gcggagaaaa aacttgggcg 60 ccgcctcgcc gagaaggtga tgtatgcgct cgccatgcac attcaaacgg cgatcaaccg 120 cctgcgggcc gggacgatcg tttcccatcc gaaactgaat gaagttcgcg ccgcctataa 180 gcaggaattc gccgtcgcgc tcgactgcct tcagctgatg gaagaaagga cgaatatcga 240 cttcccgatc gatgaagcgg gatttttgac gatgtttttt gcgttccacg aggagcaagc 300 ggaagagcgg gaggaacggg tggcgatcgt ggttgtgatg catggcaacg gtgtggcttc 360 ggcgatggcg gacgtcgtga atcagctgtt ggccgccgcg tgtgtgcatg cggtcgatat 420 gccgcttgat gccgacccga aacgaattta cgagcaggtg aaagcggtgc tacagcccgt 480 cgcatcgaaa aaaggggcgc tgttgcttgt tgacatggga tcgctcgtgt cgttttcgaa 540 ctttttggaa aaagagctcg ctgttccggt gcgggtgatt tccgctgcca gcacgccgca 600 cgtgctcgaa gcggcccgca aagcgatgct cggctatgcg cttcaggaaa tttacgaaga 660 agtgaaagcc gcagcgccgt tttacattcg cgggccgctt tgggaggagg aggctgcgga 720 gcaggacatg ctggcgatcg tcaccgcctg ttttactgga aaaggaagcg cgctggcgct 780 aaagcatata ttggaaacgt atttgcagct cgatgagcgc atgtggcgca ttattcccat 840 tcaaatggca gatgcggaag aagcgcggca aaccttgtcc aacgtcgcga aacatttccg 900 catcgtctgt atcgtcagcc atctttgcct tgatgagcga atcccgcact tttctcttga 960 ggacgtgctt agcttgaggg cgatgaaaga aatccaagcg ttagccgacg ttgaggaaat 1020 tcatatgcat atggctagag agctgaccaa tcatcttcgc cacctcgcac cggcgcgggc 1080 cattcccgcc attcgcgctg cgctggcggc gattggtcgg gaactcggcc ttgaagcgga 1140 tggccgcgat ctggtggggc ttgtctttca tctttgctgc ttgctcgatc gcctgctgtc 1200 cggagagaga acgagcggtg accgaggagc agcgaaagct tgcgggcatg aagatgaaga 1260 tggcgcactg tacagggcgg tcaaggaggg gttgtttccg cttgaacagc agtacggtgt 1320 ctgcatcgat gaagatgaac tgtgtcatat cgttcacttt tttcgctccc tgcaaggaaa 1380 acgagatgga taaggtgcga taattggaaa cgcttccatt acacagtttc cctccgttaa 1440 cacacaaccg ttccatccgc ttcagcgctg tcttttcggc gttggcatgg aatttgcttg 1500 aacaataatg aatcattgca aaaaaaggag gaggaagcgg atgaacattt tgctcatttg 1560 cgctgccggc atgtcgacga gtttgcttgt gacgaagatg aaggaggcgg caaagcaaaa 1620 agggatcgag gcgaacattt gggctgtgtc agctgatgaa gcgaaaagtc atctcgacca 1680 ggcggatgtt gtgctgattg gtccgcaaat ccgttataag ctcgccgcct tcaaaaaaga 1740 gggagaggcg cgcggcattc cggttgacgt catcaatcca gctgactatg ggcgggtcaa 1800 cggcgccggc gtgttggact ttgcgctgcg gttaaaaaaa taaacagggg gtagaagcgg 1860 atggaccggt ttattcggat gttggaagac cgcgtgatgc ctgtcgccgg caagattgcc 1920 gaacagcgcc atttgcaggc gattcgtgac gggattattt tgtcgatgcc attgttgatt 1980 atcgggtctt tatttttaat cgttggcttt ttgccgattc ccggttacaa cgaatggatg 2040 gcgaaatggt tcggcgagca ttggcttgat aagcttctct atccggttgg agcgacgttc 2100 gatattatgg cgcttgttgt cagcttcgga gtcgcctatc ggctggcgga aaagtataaa 2160 gtggatgcgc tgtccgccgg cgcgatttca ctggctgctt ttttgctcgc aaccccgtat 2220 caagtgccgt tcacgccgga aggagcgaaa gaaaccatta tggtcagcgg tggcatcccg 2280 gtgcaatggg tcggcagcaa ggggttgttt gttgccatga ttttggcgat tgtgtcaacc 2340 gaaatttacc ggaaaatcat tcaaaaaaat attgtcatta agctgccgga cggggtgccg 2400 cctgctgtgg cccgctcctt tgttgctttg atcccgggag ccgccgttct cgtcgttgtc 2460 tgggtagccc ggctgatttt ggaaatgaca ccgtttgaaa gtttccataa cattgtatct 2520 gtcctcctga acaaaccgct cagtgtgctc ggcggcagtg tatttggcgc cattgtcgcc 2580 gtgctgcttg tccagctgct atggtcgacc ggactgcacg gagcggccat cgtcggcggg 2640 gtcatggggc cgatctggct gtcgctgatg gatgaaaatc gaatggtgtt ccagcaaaat 2700 ccgaatgccg aactgcccaa cgtcattacg caacagtttt ttgatctttg gatttacatc 2760 ggcggttcag gagcgacatt ggcgttggcg ttgacgatga tgtttcgggc ccgcagccgg 2820 cagctgaaaa gcttaggtcg gctggcgatc gcgcccggca ttttcaatat taatgagccg 2880 atcacgttcg gcatgccgat cgtcatgaac ccattgctga tcattccgtt cattctcgtg 2940 cctgtcgtgc ttgtggttgt ttcctacgcc gcgatggcga ccgggcttgt cgccaaacca 3000 agcggggtgg ccgtgccatg gacgacaccg atcgtgatca gcggctattt agcgacggga 3060 ggcaaaattt ccgggagtat tttgcaaatc gttaacttct tcatcgcgtt tgccatctac 3120 tatccatttt tctcgatttg ggacaaacaa aaagcggccg aagagcaggc cgatccaacg 3180 atttcaagcg gagcgggggc aacgcactcg ctgtaaagga gaggaacgaa atgccgcgct 3240 attgcatcgt caacgccgat gatttcggtt actcgaaagg ggtcaactac gggattttgg 3300 aagcgtttca gaacggtgtc gtcacgtcgg cgacgctgat ggcgaatatg ccagcggccg 3360 aacacgccgc ccggctggcg aaggaccatc cggaactcgg cgttggcatt cattttgtgc 3420 tgacgtgcgg ccggccgctg gccgatgttc catcgctggt gaacgagaat ggggagtttc 3480 cgcggcgcgg ggaggcgctt gtcggcgcta ggcgcggcga tatcgagcgg gagctttgcg 3540 cccaattgga gcgttttttc tcgttcgggc tcactccgac gcatattgac agccatcatc 3600 acgttcatga gcacccgaat gtgtttccgg ttgtggaaca attggccgaa cgctatcggc 3660 tgccgatccg cccggtgcgg acggcacggc cgcatcggct gccgaccgtc gacgtctttt 3720 ttccggattt ttacggcgat ggactgacga aagaccgttt tatctcgctg atcgaccgaa 3780 ttggcgacgg gcagacggcg gaagtgatgt gccacccggc gtatatcgat gttccgctcg 3840 cgtcaggaag ttcctattgt caacaacgag tcgaagagct ggcggtgctg accgacccaa 3900 cgctcgttgc cgagatggcc gagcgcggtg ttcagctgat cacgtaccgc gaattctata 3960 aactatagga gagggcgtta tgcaaacgta tgaacaaact gtattccaac tgattcttca 4020 tggcggaaac ggccgcagtt atgcaatgga ggcgattacg gcggcgaaaa aaggggaatt 4080 tgccgaggcg cgcaggctgc ttgaacaggc gggagcagaa ctgcaggcgg cccatgggct 4140 gcagaccgcg ttgctgcaac aagaagcgag cggcgggcag ccggtggtga cgcttctgat 4200 ggtgcatgcc caagatcatt taatgacggc gatcacggtc aaggatttag ccgctgaatt 4260 cgtggagctg tatgaagcgc tcaagcggca aacaaccgaa tcataattgt cgtcggccgg 4320 cttgttgacc gggtgaggat gattcgcttc aacgggaatg aacccaaggc gggcggcaac 4380 gggctgttct agcgaccgcc cgctttgttg attttacaat aattttatta tgtgaacata 4440 agggcgccag ccgttcagcc ctcccggctg ccgccggtct cggctgaacg gagaaggaaa 4500 actccgcttg tttgcgtcac atatcgtggc ttgtgttgtg cttttgccgc gtgtgattgc 4560 ggttcttcac ttttttcgaa ccgtcaagcg gctccggctg cccaggattg tcgccttttg 4620 gggcgttttt gcgcatatct ttgccgtcgt ttttgttcgt catttgttgt ccctcctttg 4680 tccgttagca tgcggcgttg tgcttttgct tattcggggc ataatttgcg ccggtttgaa 4740 acgataagta gtggatgacc gcgatgactg cgaaggagga acgagccatg gtttatcata 4800 aaacgaagca agacgcgttt caggcgcggc aaaaggcgac gatggaggca aaagagtggc 4860 atgaccatct cgtccgcgat caagccgatt acggccatca gttagcccat ttgcggcagg 4920 aagtgaacga agcgtttgca caaattgaaa acgcgcttga agtcgcctcg gaaacgcagc 4980 gcatgcagct tgagaaattc cgcagcgatt tgcaggcgat ccccgg 5026 // |
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