 |
|
EBI DbfetchID U07025; SV 1; linear; mRNA; STD; PRO; 2524 BP. XX AC U07025; XX DT 16-MAR-1994 (Rel. 38, Created) DT 17-APR-2005 (Rel. 83, Last updated, Version 6) XX DE Janthinobacterium lividum chitinase (chi 69) gene, complete cds. XX KW . XX OS Janthinobacterium lividum OC Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales; OC Oxalobacteraceae; Janthinobacterium. XX RN [1] RP 1-2524 RX DOI; 10.1016/0378-1097(95)00268-A RX PUBMED; 7557339. RA Gleave A.P., Taylor R.K., Morris B.A., Greenwood D.R.; RT "Cloning and sequencing of a gene encoding the 69-kDa extracellular RT chitinase of Janthinobacterium lividum"; RL FEMS Microbiol. Lett. 131(3):279-288(1995). XX RN [2] RP 1-2524 RA Gleave A.P.; RT ; RL Submitted (22-FEB-1994) to the EMBL/GenBank/DDBJ databases. RL Andrew P. Gleave, Gene Transfer and Expression Group, Horticulture and Food RL Research Institute of New Zealand LTD, Hort research, Private Bag, RL Auckland, Auckland, 92169, New Zealand XX FH Key Location/Qualifiers FH FT source 1..2524 FT /organism="Janthinobacterium lividum" FT /mol_type="mRNA" FT /clone_lib="lambda GEM11" FT /clone="pRKT10" FT /db_xref="taxon:29581" FT -35_signal 277..280 FT /note="putative promoter region" FT -10_signal 299..304 FT /note="putative promoter region" FT RBS 382..385 FT sig_peptide 392..460 FT /gene="chi 69" FT /note="putative" FT CDS 392..2389 FT /codon_start=1 FT /transl_table=11 FT /gene="chi 69" FT /product="chitinase" FT /function="hydrolyses 4-methylumbelliferyl beta-D N,N',N'' FT chititrioside" FT /note="estimated molecular mass 69 kDa" FT /db_xref="GOA:Q48373" FT /db_xref="HSSP:1KFW" FT /db_xref="InterPro:IPR013781" FT /db_xref="UniProtKB/TrEMBL:Q48373" FT /protein_id="AAA83223.1" FT /translation="MENLILKKTIRNMMVCLASGGVLAACGGGGGAGDAGAPAGSQLLA FT ASTVVACVPWQEGGTYNAGTVVTYLGGNYTALVTQTDHVGSGWNPVSTPSLWSAGGTCD FT GGTTPTPTPTPTPTPTPTPTPTPTPTPEPTPTPTPTPTPSPTGGTCALAWAAGTAYSAG FT ATVSYAGTNYRANYWTQGDNPSTSSGGAGTGKPWTSQEICSTTPTPTPTPTPTPTPTPT FT PTPTPTPTPTPTPTPTPTPTPTGREVGSYFAQWGVYGRDYEVANVHTSGVADKLTFINY FT AFGNIYPKNGGYECGMINKAEPGATNPNSPDTGTGGDAEADYIRTPKRTVDGQVIPWDA FT PLSGNFLQLKKLKAAHPNLKLFISLGGWSWSKNFSVGSATDALRKQMVRSCIDIYIKGN FT LPVQGGRGGVGAAANIFDGIDIDWEYPVGGGQPYNTVSPNDKRNFTLLMAEFRSQLDAL FT GAANGKRYLLTAAVGAGKDKIDNTEPALYSQYMDWINLMTYDFHGGWETSTNFNAQLFA FT DPADPSTGMAREYVGDKAVQYMIAAGVPRDKLLLGIPFYGRGWTGVAPGPNGDGLYQAA FT TGTAPGTYESGIEDYKILVAKAGTRYYHPVTKQLYLYTGAGGQWWSYDDPTVIGTKVKY FT VKDQGLRGAFSWELDGDANGVLATEVWKVR" FT misc_feature 554..682 FT /gene="chi 69" FT /note="chitin-binding domain, amino acids 55-97" FT misc_feature 851..952 FT /gene="chi 69" FT /note="chitin-binding domain, amino acids 154-187" FT repeat_region 2409..2435 FT /rpt_type=INVERTED XX SQ Sequence 2524 BP; 523 A; 799 C; 779 G; 423 T; 0 other; atcgatggct gtatttgatt tgcctcagta agtctgtctg atacggcggg tggccaggca 60 ggcacccgcc gttgccgcat tcagcggcgc agaaagctgt cgcaatacca cttacaccgt 120 gttataaaac tggtctgtct tttcccgttc cagcgtcttt tttcatcctc cctctgccgt 180 tttttcattg tccagtagga cagcacgccg tttgcaccca tcgtgcgctt tctgttcgaa 240 gtggtattgc tcactttgat gccacttcaa aaccacttga ccgcctattt tttcagcagc 300 atgctgttct tgcaactgca aaaaaacaag atcacagggt agttatttac gaggcagagt 360 ggcatttctg caaacctgaa caggagacaa cgtggaaaat ttaatactca aaaaaacaat 420 caggaacatg atggtatgtc tggccagcgg cggcgtgctg gcggcatgcg gtggcggtgg 480 cggtgccggt gacgccggcg cgccggctgg ttcgcaattg ctggcggcca gcacggtcgt 540 cgcttgcgtg ccgtggcagg agggcggcac ctacaacgcg ggcacggtgg tcacgtatct 600 ggggggcaac tacacggccc tggtgacgca gaccgatcac gtgggctcgg gctggaatcc 660 cgtctccacg cctagcctgt ggagcgccgg cggcacctgc gacggcggca ccacaccgac 720 cccgacgccg actcccaccc cgacgccgac gcccaccccg acgccgacgc ccaccccgac 780 accggagcca acgccgaccc caacgcctac gccgaccccg agcccaaccg gcggcacctg 840 cgcactggcg tgggcggccg gcacggctta cagtgcgggc gccaccgtca gctatgcggg 900 cacgaattac cgcgccaatt actggacgca gggcgacaac ccgtcgacca gcagcggcgg 960 cgccggcacg ggcaagccat ggaccagcca ggagatctgc agcaccacgc cgaccccgac 1020 cccgacgccg acgccgacgc ctactccaac accgactccg acacctacac cgacgcctac 1080 tccaacgccg acacctactc caaccccaac gccgacccca acgggccgcg aagtcggttc 1140 ctatttcgcg cagtggggcg tgtatggccg cgactatgaa gtggcgaacg tgcacacgag 1200 cggcgtggct gacaagctga ccttcatcaa ctacgccttc ggcaatattt acccgaaaaa 1260 cggcggttat gaatgcggca tgatcaacaa ggccgagccg ggcgcgacaa atccgaattc 1320 gcccgatacg ggcacgggcg gcgatgccga ggccgactac atccgcacgc cgaagcgcac 1380 cgtcgatggg caggtcattc catgggacgc tccattgtcg ggcaacttcc tgcagctgaa 1440 aaagttgaag gcggcgcacc cgaacctgaa gctgttcatt tcgctgggcg gctggagctg 1500 gtcgaagaac ttctccgtcg gttcggccac ggatgcgctg cgcaagcaga tggtgcgctc 1560 gtgcatcgac atctacatca agggcaacct gccggtgcag ggcggtcgcg gcggcgtggg 1620 cgcggcagcc aacatctttg acggcatcga catcgattgg gaatatccgg tcggcggcgg 1680 ccagccgtac aacacggtca gcccgaatga caagcgcaac ttcaccttgc tgatggcgga 1740 attccgcagc cagctcgatg cgctcggtgc ggccaacggc aagcgctacc tgttgacggc 1800 ggctgtcggc gccggcaagg acaagatcga caacacggaa ccggccctgt actcgcagta 1860 catggactgg atcaacctga tgacctacga cttccacggc ggctgggaaa cgagcaccaa 1920 cttcaacgcc cagctgtttg ccgaccctgc cgatccgtcg acgggcatgg cgcgcgaata 1980 cgtgggcgac aaggcggtgc agtacatgat tgccgccggc gtgccgcgcg acaaactgct 2040 gctgggcata ccgttctatg gccgcggctg gacgggcgtg gcgccagggc cgaacggcga 2100 tggcctgtac caggcggcca cgggcacggc gccgggcacc tacgagtcgg gcatcgagga 2160 ctataaaatc ctcgtggcca aggccggtac gcgctactac catcccgtga ccaagcagct 2220 gtacctgtac acgggtgcgg gcggccagtg gtggagctat gacgatccga ccgtcatcgg 2280 caccaaggtg aaatatgtga aggaccaggg cttgcgcggc gcgttctcgt gggaactcga 2340 tggcgacgcg aatggcgtgc tggcgaccga ggtgtggaaa gtgcggtaac actggagtag 2400 agtaagaccg gccgggcggc attgctgccc ggccgcttgt tcacataaaa aacaggagac 2460 gggcgatggc ggcggtatcg acattcggag taaaacatcc ggaactggag ctgagccgcg 2520 agtg 2524 // |
|