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EBIDatabasesDatabase BrowsingDbfetch

EBI Dbfetch

ID   U01901; SV 1; linear; mRNA; STD; PLN; 1296 BP.
XX
AC   U01901;
XX
DT   23-NOV-1993 (Rel. 37, Created)
DT   17-APR-2005 (Rel. 83, Last updated, Version 4)
XX
DE   Solanum tuberosum Datura endo-1,3-beta-D-glucanase (gluB2) mRNA, complete
DE   cds.
XX
KW   .
XX
OS   Solanum tuberosum (potato)
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliophyta; eudicotyledons; core eudicotyledons;
OC   asterids; lamiids; Solanales; Solanaceae; Solanoideae; Solaneae; Solanum.
XX
RN   [1]
RP   1-1296
RX   DOI; 10.1007/BF00020173
RX   PUBMED; 8111037.
RA   Beerhues L., Kombrink E.;
RT   "Primary structure and expression of mRNAs encoding basic chitinase and
RT   1,3-beta-glucanase in potato";
RL   Plant Mol. Biol. 24(2):353-367(1994).
XX
RN   [2]
RP   1-1296
RA   Kombrink E.;
RT   ;
RL   Submitted (17-SEP-1993) to the EMBL/GenBank/DDBJ databases.
RL   Erich Kombrink Dr., Abteilung Biochemie, Max-Planck-Institut fuer
RL   Zuechtungsforschung, Carl-von-Linne-Weg 10, Koeln, D-50829, Germany
XX
FH   Key             Location/Qualifiers
FH
FT   source          1..1296
FT                   /organism="Solanum tuberosum"
FT                   /cultivar="Datura"
FT                   /mol_type="mRNA"
FT                   /dev_stage="six-week-old plant"
FT                   /clone_lib="cDNA library of elicitor-treated potato leaves
FT                   in lambda NM1149, pUC9"
FT                   /clone="GluB2"
FT                   /tissue_type="leaf"
FT                   /db_xref="taxon:4113"
FT   misc_feature    11..1099
FT                   /gene="glub2"
FT                   /note="preproprotein"
FT   CDS             11..1102
FT                   /codon_start=1
FT                   /gene="glub2"
FT                   /product="1,3-beta-D-glucan glucanohydrolase;
FT                   endo-1,3-beta-D-glucanase; 1,3-beta-glucanase (basic, class
FT                   I)"
FT                   /function="catalyzes the hydrolysis of 1,3-beta-D-glucoside
FT                   linkages in 1,3,-beta-D-glucans (such as laminarin)"
FT                   /EC_number="3.2.1.39"
FT                   /note="plant defense gene; induced expression in response
FT                   to infection, elicitor, ethylene, wounding"
FT                   /db_xref="GOA:P52401"
FT                   /db_xref="HSSP:1GHS"
FT                   /db_xref="InterPro:IPR013781"
FT                   /db_xref="UniProtKB/Swiss-Prot:P52401"
FT                   /protein_id="AAA18928.1"
FT                   /translation="MATSQIAVIVLLGLLVATNIHITEAQLGVCYGMMGNNLPSHSEVI
FT                   QLYKSRNIGRLRLYDPNQGALNALRGSNIEVILGLPNVDVKHIASGMEHARWWVQKNVK
FT                   DFWPDVKIKYIAVGNEISPVTGTSSLTSFQVPALVNIYKAVGEAGLGNDIKVSTSVDMT
FT                   LIGNSYPPSQGSFRNDVRWFTDPIVGFLRDTRAPLLVNIYPYFSYSGNPGQISLPYALF
FT                   TAPNVVVQDGSRQYRNLFDAMLDSVYAAMERTGGGSVGIVVSECGWPSAGAFGATQDNA
FT                   ATYLRNLIQHAKEGSPRKPGPIETYIFAMFDENNKNPELEKHFGLFSPNKQPKYNLNFG
FT                   VSERVWDISAETNSTASSLISEM"
FT   misc_feature    11..85
FT                   /gene="glub2"
FT                   /note="leader peptide"
FT   mat_peptide     86..1030
FT                   /gene="glub2"
FT                   /product="1,3-beta-D-glucan glucanohydrolase;
FT                   endo-1,3-beta-D-glucanase, 1,3-beta-glucanase (basic, class
FT                   I)"
FT                   /note="preproprotein is processed to the mature protein by
FT                   deletion of the leader peptide and the C-terminal
FT                   extension."
FT   misc_feature    1031..1099
FT                   /gene="glub2"
FT                   /note="C-terminal extension"
FT   misc_feature    1067..1075
FT                   /gene="glub2"
FT                   /note="N-glycosylation consensus site"
FT   polyA_signal    1180..1185
FT   polyA_site      1294
XX
SQ   Sequence 1296 BP; 384 A; 226 C; 274 G; 412 T; 0 other;
     atcaaacatc atggctacct cacaaatagc tgttatcgtg cttctaggat tacttgttgc        60
     caccaacatt cacattacag aggctcaatt aggtgtttgc tatggaatga tggggaacaa       120
     cttgccatca cattccgaag ttatacagct ctacaagtca agaaacattg gaagattgag       180
     gctttatgat ccgaatcaag gagctttaaa tgcgttaaga ggatcaaaca ttgaagtgat       240
     actaggactt ccaaatgtag atgtgaaaca cattgcttct gggatggaac acgcgagatg       300
     gtgggtacag aagaacgtta aagatttctg gcctgatgtt aaaattaagt acatagctgt       360
     tggtaatgaa atcagccctg ttactggcac atcgtctctt acctcatttc aagttcctgc       420
     tttggttaac atttataaag cagtcggtga agctggtttg ggaaatgaca ttaaggtttc       480
     aacatcagta gacatgacgt tgattggcaa ttcttatcca ccatcacaag gttcttttag       540
     gaacgatgtt agatggttca ctgatccgat tgttgggttt ttaagggata cacgtgcacc       600
     tttgctcgtt aacatttatc cttattttag ctattctggt aatccaggac agatttcact       660
     tccgtatgct ctttttacag cacctaatgt ggtggtacaa gatggatcac gtcaatatag       720
     gaacttattt gatgctatgt tggattctgt ttatgctgcg atggaacgaa caggaggagg       780
     atctgtagga attgttgtgt cagagtgtgg atggccgtct gctggtgcat ttggtgccac       840
     acaagacaat gcagcaacat acttgaggaa cttaattcaa catgcgaaag aaggtagtcc       900
     gagaaagcct ggacctatcg agacttatat attcgccatg tttgatgaaa ataacaagaa       960
     tccagagctt gagaaacatt ttggattgtt ttccccaaac aagcagccaa aatataacct      1020
     aaactttggg gtgtctgaga gagtttggga catttctgct gaaactaata gcactgcttc      1080
     ttccctcata agtgagatgt aagaagacac atttgaaagc atatagtatc ggcaaaaata      1140
     cttattcttc atgcaatttt atgtttgtat gcaacatcaa ataaattcct agtggtgttt      1200
     taaatttcct tttgtttgtg attttgggaa gaaacttttt gtaagtttgt atatatttct      1260
     tacaccatca attacattat ttccatgttt gattaa                                1296
//
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