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EBI DbfetchID U01232; SV 1; linear; genomic DNA; STD; PRO; 4201 BP. XX AC U01232; XX DT 21-NOV-1993 (Rel. 37, Created) DT 17-APR-2005 (Rel. 83, Last updated, Version 7) XX DE Streptomyces albus SalI restriction endonuclease (salIR) and SalI DE modification methyltransferase (salIM) genes, complete cds. XX KW . XX OS Streptomyces albus OC Bacteria; Actinobacteria; Actinobacteridae; Actinomycetales; OC Streptomycineae; Streptomycetaceae; Streptomyces. XX RN [1] RP 1-4201 RX DOI; 10.1016/0378-1119(94)90650-5 RX PUBMED; 7828868. RA Rodicio M.R., Quinton-Jager T., Moran L.S., Slatko B.E., Wilson G.G.; RT "Organization and sequence of the SalI restriction-modification system"; RL Gene 151(1-2):167-172(1994). XX RN [2] RP 1-4201 RA Wilson G.G.; RT ; RL Submitted (02-SEP-1993) to the EMBL/GenBank/DDBJ databases. RL Geoffrey G. Wilson, New England Biolabs, Inc., 32 Tozer Road, Beverly, MA RL 01915 USA XX FH Key Location/Qualifiers FH FT source 1..4201 FT /organism="Streptomyces albus" FT /strain="G" FT /mol_type="genomic DNA" FT /clone_lib="PstI fragments" FT /clone="pGW104-9" FT /db_xref="taxon:1888" FT CDS 1008..1955 FT /codon_start=1 FT /transl_table=11 FT /transl_except=(pos:1008..1010,aa:Met) FT /gene="salIR" FT /standard_name="R.SalI" FT /product="SalI restriction endonuclease" FT /function="cleaves ds DNA at GTCGAC forming G'TCGAC" FT /note="complete CDS, aa 1-315; GTG start codon" FT /db_xref="GOA:Q53608" FT /db_xref="InterPro:IPR019072" FT /db_xref="UniProtKB/Swiss-Prot:Q53608" FT /protein_id="AAA81886.1" FT /translation="MINADKPHRWNDDVQASVRLYNQWFLDAAPKAYRDTRQLTIDEVE FT QAFQRTANMTSITPEVLKAHPKTLATLRMSTAPPIARDRLVGLSHGSKSLLDTMEKGKL FT PPRMKGDVLDTHLAKMCAVLTDLLDLDLFHWYPTGEPAEPRQRELAATVVADRLCGAIA FT DPIVRNAQERRQLALIEEWLLARGYTKKTHSASLPLNTMQPGTFSFRQNVVVGSDLPVN FT IPVDAVIQPHTPHSHKLPILIEAKSAGDFTNTNKRRKEEATKIHQLQLKYGNEISLTLF FT LCGYFNTGYLGYSAAEGLDWVWEHRIDDLEAAGA" FT CDS 1948..3711 FT /codon_start=1 FT /transl_table=11 FT /transl_except=(pos:1948..1950,aa:Met) FT /gene="salIM" FT /standard_name="M.SalI" FT /product="SalI modification methyltransferase" FT /function="methylates ds DNA at GTCGAC forming GTCGm6AC. FT Member of the m6A-gamma class of DNA methyltransferases. FT Requires S-adenosylmethionine as co-factor" FT /note="complete CDS, aa 1-587; GTG start codon" FT /db_xref="GOA:Q53609" FT /db_xref="InterPro:IPR002052" FT /db_xref="InterPro:IPR002296" FT /db_xref="UniProtKB/Swiss-Prot:Q53609" FT /protein_id="AAA81887.1" FT /translation="MHSEAREAEARRLLLQETLDAERTFLERNQWGQFATPPSLAGEIM FT RYTIDLHEETRINFLEPSCGSGSFFSALLRNLGDKKIEHAVGVELDPRFSKAASDLWTG FT QGLRVIEGDFTSPSLVSGPVASLLVANPPYVRHHHLGIDQKRDLVARCADQLGIKPSGL FT SGLYLYFVLLSHRLLRADAVSTWLIPSEFMDVNYGTALKEYLATRVQLVRIHQYDAAEV FT QFDDALVTSSVVVFRNSPPRPGHTAEFSFGGTLSEPKVTHQIPSAALTPEAKWSRYVTG FT VMPADINLKQTGPKLSDFFKIRRGLATGSNAFFIIPRSEAERLGIKRNFLRPILPSPRK FT LKGDAITADASGWPDIPEQLALLDCPLPIEDLLLENPALAAYLSTADEKIRGGYLVSKR FT SPWYKQEQREPAPILLTYMGRGKDDQHPLRFIRNDSDAVATNMYLMLYPTALLQRYLAG FT DPERIKQVHKALLAITAADLRGGGRVYGGGLHKMEPKELAALPADGIATLDPVLREDIS FT MVSVPPRKRTGRPQMPGPSASEVRAWARANGVCVPDRGRLRPEVWDAWRQAHAGEASPL FT NIDAGDQVALW" XX SQ Sequence 4201 BP; 890 A; 1306 C; 1190 G; 815 T; 0 other; ctgcagcaca aggcaaaggg aaagaaaaaa ccccaggtca cggcgagtga gtcctggggt 60 aattccgagc cgccttcggg attcgaaccc gagacctacg cattacgagg gcctgcaaga 120 tcgtgacggg ccgtaccgga tgatcgcgct aaatcctgtt cttcctgatc agaggctcct 180 cgctggctcg cgtctgccgc gtcgtccagc aaggtgctgg cccgtccgct cacgcatcgc 240 tcacgcactc ggaggacaac gttagttgat ggagaccagt ttcctaccgg accctaacgc 300 taagggtgtg caggcagtag ctcgtagcta ccgatggcgt ccgcataggg cagaaggcgc 360 gactccagct cccgatgaag cagctcagca tcctgcgccg acagcgcaag cgtcgtccgt 420 ctgagttgca cagcccaggc ccgcttgttg gggaatgcat cgagcgcgtg caccaagtct 480 cggatgctca gtccttcgag gaagggggcg agcccctgaa tctgaatccc acatcccgtg 540 gtgaactccc ggtctccgaa tctgactggt gccgtcaaga cgctcacggg cgccgccacc 600 gaaccacgcc caatgatacg gccaacgtca cgggtcgggt tgcgaaagca gccacgtgtg 660 gtgtatagca ggacttcgtc acccacccgc aggacgccac tctgtcgtgc ccgccccgcc 720 gggaaagcca gacgctgctc ggtcaaaacc cagcgcaagg cggagcgctc gccgaggatt 780 acgaggtgcg tggaggccat acaggtcact ttagcggtgc ctcaaccatc cggcggagcc 840 cccgtccgcg ctgacaccac ccgccgcgcc ggcacgcttc gttgccaagc tccacggagc 900 agggtgatca cgccggcccg actccctgcg ccttctacgt ccgtaaactg gcacaacggc 960 actgcgcatc aagcctcccc ccgactccta cccgaaggta cgtttaagtg atcaacgctg 1020 acaagccgca ccgctggaac gatgatgtcc aggcttccgt gcgcctgtac aaccagtggt 1080 ttctagatgc tgctccaaag gcttaccggg atactcggca actcacgatc gacgaggtgg 1140 aacaggcttt ccagcggacg gccaatatga cgtcgatcac acctgaggta ctaaaagccc 1200 accctaagac cttggcgact ctccgcatga gcacagcgcc tcccattgct agggaccgtc 1260 tggtcggcct ttcgcacggc agcaagtcac tgcttgacac gatggagaag ggaaagctcc 1320 cacccaggat gaagggggac gtgctcgaca cgcatctcgc gaagatgtgc gccgtcctca 1380 cggatcttct ggacttggac ctgttccact ggtaccccac gggagaacca gcagagccgc 1440 gccagcgtga actggcagca accgtcgttg cggatcgcct ctgcggcgcc atagctgatc 1500 ccatcgtcag gaacgcccag gagcgcaggc agctagcctt gattgaagag tggcttcttg 1560 cgcgcggata cacgaaaaaa acacactcgg cttcacttcc actgaacacc atgcagccag 1620 gtaccttttc gtttcggcag aacgtggtgg ttgggagcga cctcccagta aatattcccg 1680 ttgatgccgt catccagcca cacaccccgc acagccacaa gctccctatc ctaatagagg 1740 ccaagtcggc gggcgacttc actaacacca ataagcgccg caaagaggaa gccaccaaaa 1800 tacaccagct tcagcttaaa tatggtaacg aaatttcact gaccctcttc ctctgtggat 1860 atttcaacac cggatacctt gggtattcgg cagcagaggg cctggattgg gtatgggagc 1920 atcgaattga cgacctggag gccgctggtg catagcgagg cgagagaggc cgaagcacgc 1980 aggctactgc tacaggaaac cttggatgca gaacgcactt tcctagagcg aaatcagtgg 2040 ggccagttcg ccacaccacc ctccctggca ggggagatca tgcgctacac cattgacctg 2100 catgaagaaa cgcgcatcaa tttccttgag ccttcttgcg gatctggctc gttcttctcg 2160 gccctgctgc gaaatttggg cgacaagaaa attgagcacg cagtcggtgt cgagctagat 2220 cctcgcttct ccaaggccgc ctctgatctc tggacggggc agggtctgcg tgtgatcgaa 2280 ggtgatttca cgtcaccaag tctggtcagt ggcccggtcg catcgttgct ggtcgcgaat 2340 ccgccatacg ttcgacacca ccacctgggt atcgatcaaa agcgtgattt ggtcgctcgc 2400 tgtgcagacc aactgggaat caagccttcc ggactatccg gcctgtatct gtatttcgtc 2460 cttctgagcc accgcctgct ccgcgccgat gcagtgtcta cgtggctcat cccaagcgag 2520 ttcatggacg tcaactacgg aactgctctg aaagaatatc tagcaactcg agtacaactc 2580 gttcgaatcc accagtacga cgccgcggag gtacaattcg acgacgccct ggtaacgtca 2640 agcgtcgtcg tatttaggaa cagcccgccc cgcccagggc acacagcgga gttctctttc 2700 ggtgggacac tttctgaacc caaggtcacc catcaaatcc cgagtgcggc attgaccccg 2760 gaggctaaat ggtcccgata tgtcaccggc gtcatgccag cagacattaa cctcaagcag 2820 accggaccca agctttccga tttttttaag atccgccgag ggctagctac tggctccaac 2880 gccttcttca tcatccctcg tagcgaggca gaaaggctcg gaattaagcg taacttcctg 2940 cggccgatcc tccccagtcc gagaaaactc aagggcgacg cgattacagc ggatgcctct 3000 gggtggccag acattcccga acagctcgcc ttgctcgact gtccacttcc aatcgaggat 3060 ctcctcctag agaaccctgc attggccgcc tatctgagca ctgcagatga aaagatccgt 3120 ggcggctatc tcgtaagcaa acggtcccct tggtataagc aggagcagcg agaacccgct 3180 cccatcctac tgacctacat gggtagaggt aaggacgatc aacatcccct ccggtttatc 3240 cgcaacgact ccgacgcggt ggccactaac atgtacttga tgctctaccc aacagctcta 3300 ttgcaacgtt acttggcagg agacccggag cgaatcaagc aggtacataa agccctccta 3360 gccatcaccg ccgcagatct gcgcggcgga ggcagggtgt atggcggtgg gctgcataag 3420 atggagccca aggaacttgc ggcactacca gccgacggca tcgctaccct cgaccccgtg 3480 ctccgcgagg acatctcgat ggtgtcggtc cctccgcgta aaaggactgg ccgcccgcag 3540 atgcccgggc cgtctgccag cgaagttcgg gcctgggcac gcgcaaacgg tgtgtgcgta 3600 cccgatcgcg gtcggcttcg cccagaagtg tgggatgcct ggcgacaggc acatgcaggc 3660 gaagcatctc ccctgaatat cgatgcaggg gatcaagtcg ctttgtggtg aaagcaggaa 3720 ccttcggata ctagccgttt cgaagcctac gcatccgcgt tacgctttcg ggggactcgc 3780 accagcctga accagttggt cctgccaacc tagccattcg tgttccgcag tcacagctcg 3840 tgtagggcgg cgaggacctg ccccatcagc agcggcgtga ttccgggacc gccgccggcg 3900 gggccggtga tctcttcgtt gggttgcgtt ggcacttcgg cccagacggt ctttcctggc 3960 ggggagggct ctgggtagta gccccagcag ttgggtctgc tgcaccttca ggtgacaggt 4020 tcagtcacgc ggcctgaagg gccggtgtgg tcatgacggt ctcgtattcg acgggtgtga 4080 gtcggcccag tcctatttgt ctgcggcgtc ggtggtaggt ccgttcgatc caggtcacga 4140 tcgcgattcg gagttcttcg cgagtggccc aggtgcggcg atcgaggatg ttcttctgca 4200 g 4201 // |
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