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EBIDatabasesDatabase BrowsingDbfetch

EBI Dbfetch

ID   S78087; SV 1; linear; mRNA; STD; PLN; 1794 BP.
XX
AC   S78087;
XX
DT   27-SEP-1995 (Rel. 45, Created)
DT   17-APR-2005 (Rel. 83, Last updated, Version 4)
XX
DE   dihydrofolate reductase-thymidylate synthase=bifunctional enzyme [Glycine
DE   max, seedling, mRNA, 1794 nt].
XX
KW   .
XX
OS   Glycine max (soybean)
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliophyta; eudicotyledons; core eudicotyledons; rosids;
OC   eurosids I; Fabales; Fabaceae; Papilionoideae; Phaseoleae; Glycine.
XX
RN   [1]
RC   GenBank staff at the National Library of Medicine created this entry [NCBI
RC   gibbsq 166831] from the original journal article.
RP   1-1794
RX   DOI; 10.1016/0167-4781(94)00251-W
RX   PUBMED; 7742362.
RA   Wang M., Ratnam S., Freisheim J.H.;
RT   "Cloning, nucleotide sequence and expression of the bifunctional
RT   dihydrofolate reductase-thymidylate synthase from Glycine max";
RL   Biochim. Biophys. Acta 1261(3):325-336(1995).
XX
FH   Key             Location/Qualifiers
FH
FT   source          1..1794
FT                   /organism="Glycine max"
FT                   /mol_type="mRNA"
FT                   /db_xref="taxon:3847"
FT   gene            1..1794
FT                   /gene="dihydrofolate reductase-thymidylate synthase,
FT                   DHFR-TS"
FT   CDS             156..1748
FT                   /codon_start=1
FT                   /gene="dihydrofolate reductase-thymidylate synthase,
FT                   DHFR-TS"
FT                   /product="dihydrofolate reductase-thymidylate synthase"
FT                   /note="bifunctional enzyme; EC 2.1.1.45; Method: conceptual
FT                   translation with partial peptide sequencing; DHFR-TS"
FT                   /db_xref="GOA:P51820"
FT                   /db_xref="HSSP:1HW4"
FT                   /db_xref="InterPro:IPR012262"
FT                   /db_xref="UniProtKB/Swiss-Prot:P51820"
FT                   /protein_id="AAB34317.1"
FT                   /translation="MPSDSSVISNGHSNGSVNPLPNLQRTYQVVVAATQDWGIGKDGKL
FT                   PWRLPTDLKFFKEITMKTSEPGKKNAIVMGRKTWESIPLEYRPLSGRLNVVLTRSGSFD
FT                   IATAENVVICGSMSSALELLAASPYSLSIEKVFVIGGGQIFREALNVPGCEAIHLTEIQ
FT                   SSIECDTFMPPVDFTIFRPWYSSFPKVENNIRYSFTTYVRVRSSAAESAGQNIDPLLDN
FT                   NSESMKFEVKDFSFLPKMISERHEEYLYLKLVQDIIAEGTTKGDRTGTGTLSKFGCQMR
FT                   FNLRGNFPLLTTKKVFWRGVVEELLWFISGSTNAKVLQEKGIHIWDGNASREYLDGVGL
FT                   TEREEGDLGPVYGFQWRHFGARYTDMHHDYSGQGFDQLLDVINKIKRNPDDRRIILSAW
FT                   NPVDLKLMALPPCHMFAQFYVAHGELSCQMYQRSADMGLGIPFNIASYALLTCMIAHVC
FT                   DLIPGDFIHVIGDAHIYRNHVRPLQEQLHNQPKPFPTLKINPKKKDIDSFVAADFKLIG
FT                   YDPHQKIDMKLSV"
XX
SQ   Sequence 1794 BP; 461 A; 352 C; 410 G; 571 T; 0 other;
     agttcgtgga attttgcacc tttttaggtc ataatccttt caaaattccg tgcttcggct        60
     tgacatcgat ttctctgcac ttcccaaaga attggttgtg cagtccttgt tttcagacac       120
     cgtctttaag ttactgtcga cgtttgagtt ctataatgcc cagtgattct tctgtaatct       180
     ccaatggaca cagcaatggc agtgtcaatc cactacctaa cctgcaaagg acttaccaag       240
     tggtagtggc tgccactcaa gattggggca tcggtaagga tgggaaatta ccctggagat       300
     tgcctactga tctcaagttt tttaaggaga tcactatgaa aacatctgag cctgggaaga       360
     agaatgccat tgtaatggga aggaaaacat gggagagcat ccctcttgag tacaggcctc       420
     tttctggtcg ccttaatgtt gttcttactc gctcaggaag ttttgatatt gcaacagcag       480
     agaatgttgt tatatgtgga agcatgtctt ctgcattgga actgttagct gcttctcctt       540
     actctctgtc aattgagaaa gtttttgtta tagggggtgg tcagatattt agagaggctc       600
     tcaatgtgcc tggatgtgaa gctatccact taacggaaat tcagtcaagc attgagtgtg       660
     acacttttat gcctccagtt gatttcacta tatttcggcc atggtactca tcctttccta       720
     aagtggaaaa caacatccgc tattctttca ccacttatgt gcgtgtacgg agttctgctg       780
     cagagtccgc gggtcagaac attgatccac ttcttgataa taattctgag tccatgaaat       840
     ttgaggtcaa ggatttttct tttcttccta aaatgatttc tgagagacat gaggagtatc       900
     tgtatcttaa gctggttcag gacatcattg ctgaaggtac aactaagggt gataggacag       960
     ggactggtac cttgtcaaaa tttggttgcc agatgaggtt caatctgcgt ggaaactttc      1020
     ctcttctaac aactaagaaa gtattttggc gaggggttgt tgaagagctt ctttggttta      1080
     tcagtggctc aacaaatgcc aaggtgctac aggaaaaagg cattcatatt tgggatggca      1140
     atgcatccag agaatacctt gatggtgttg gtttgacaga gcgggaggag ggtgacttgg      1200
     gacctgttta tgggtttcaa tggaggcact ttggtgccag atatactgac atgcatcatg      1260
     actactccgg ccaagggttt gatcaacttt tagatgttat taacaagata aagcgtaatc      1320
     ctgatgatcg gcgaatcatt ctctctgcat ggaatccagt ggatcttaaa ttaatggcac      1380
     ttccaccctg ccacatgttt gcacagtttt atgtagcaca cggggagtta tcatgtcaaa      1440
     tgtatcagcg atctgctgat atgggcctgg gcattccatt taatattgca tcttatgccc      1500
     ttttgacatg catgattgct catgtttgtg atctcattcc tggtgatttt attcatgtta      1560
     ttggggatgc tcatatttac cgcaatcacg tgaggccttt gcaggagcag ctccataacc      1620
     agccaaagcc ttttccaact ttgaagataa atccaaagaa gaaagatata gattcttttg      1680
     tggctgctga tttcaagctc ataggctatg atcctcacca gaagattgat atgaagctgt      1740
     ctgtctaaaa tctggggatt ctcactcctt caggttgttt ctgctttttg aaat            1794
//
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