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EBIDatabasesDatabase BrowsingDbfetch

EBI Dbfetch

ID   M98832; SV 1; linear; genomic DNA; STD; FUN; 1700 BP.
XX
AC   M98832;
XX
DT   07-OCT-1992 (Rel. 33, Created)
DT   17-APR-2005 (Rel. 83, Last updated, Version 5)
XX
DE   Candida boidinii 3-isoproypylamalate dehydrogenase (LEU2) gene, complete
DE   cds.
XX
KW   3-isopropylmalate dehydrogenase; auxotrophic marker gene; LEU2 gene.
XX
OS   Candida boidinii
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC   Saccharomycetales; mitosporic Saccharomycetales; Candida.
XX
RN   [1]
RP   1-1700
RX   PUBMED; 1522074.
RA   Sakai Y., Tani Y.;
RT   "Directed mutagenesis in an asporogenous methylotrophic yeast: cloning,
RT   sequencing, and one-step gene disruption of the 3-isopropylmalate
RT   dehydrogenase gene (LEU2) of Candida boidinii to derive doubly auxotrophic
RT   marker strains";
RL   J. Bacteriol. 174(18):5988-5993(1992).
XX
FH   Key             Location/Qualifiers
FH
FT   source          1..1700
FT                   /organism="Candida boidinii"
FT                   /strain="S2 AOU-1"
FT                   /mol_type="genomic DNA"
FT                   /db_xref="taxon:5477"
FT   misc_signal     16..32
FT                   /gene="LEU2"
FT                   /standard_name="control segment"
FT                   /note="putative"
FT   -35_signal      38..44
FT                   /gene="LEU2"
FT                   /note="homologous to E.coli"
FT   -10_signal      63..69
FT                   /gene="LEU2"
FT                   /note="homologous to E.coli"
FT   mRNA            <321..>1596
FT                   /gene="LEU2"
FT   CDS             321..1418
FT                   /codon_start=1
FT                   /gene="LEU2"
FT                   /product="3-isopropylmalate dehydrogenase"
FT                   /db_xref="GOA:Q01987"
FT                   /db_xref="HSSP:2AYQ"
FT                   /db_xref="InterPro:IPR004429"
FT                   /db_xref="UniProtKB/Swiss-Prot:Q01987"
FT                   /protein_id="AAA34349.1"
FT                   /translation="MSIIEKKIVLLPGDHVGVEVVEEAVKILKSISEVKPEIQFKFENH
FT                   LIGGAAIDATGVPLPDEALEAAKKSDAVLLGAVGGPKWGTGEVRPEQGLLKIRKELNLY
FT                   ANLRPCNFASDKLLDLSPLKSDIVKGTDFTVVRELVGGIYFGDRVEDDGSGFASDSESY
FT                   SVPEVERITRMAAFLSLQNDPPLPIWSLDKANVLASSRLWRKTVDRVIKEEFPKLTVQH
FT                   QLIDSAAMILVKSPTKLNGIVITNNMFGDIISDEASVIPGSLGLLPSASLASLPDTNQA
FT                   FGLYEPCHGSAPDLPKNKVNPIATILSAAMMLKLSLNLVKEGNAVEEAVRKVLDQGIMT
FT                   GDLGGNNSTTEVGDAIAKEVKLLLA"
FT   terminator      1559..1596
FT                   /gene="LEU2"
FT                   /note="putative"
XX
SQ   Sequence 1700 BP; 614 A; 170 C; 294 G; 622 T; 0 other;
     agaatgagaa aagttgccgg taccggaacc atatttcttg attacataag ccatagtaat        60
     attatattag tacaatataa gttattatta acacaatcaa ttattcaatt ttcaaaatta       120
     attgagaaaa ttgggaatta gttatatgac tgactaactt attcaacttg tggagaaaat       180
     agagtatgaa agttgttcat atatataagt taattattca tttttttaat ttaaataatt       240
     aagtatttaa tatatttaag tatatatata tatatatata tatttgagta tagtatagta       300
     tagtatataa gtatattaaa atgagtataa ttgaaaaaaa aattgtttta ttaccaggtg       360
     atcatgttgg tgttgaagtt gttgaagaag cagttaaaat tttaaaatca attagtgaag       420
     ttaaaccaga gattcaattt aaatttgaga atcatttaat tggtggtgca gcaattgatg       480
     ctacaggtgt tccattacct gatgaagcat tagaagcagc taaaaaatca gatgctgtat       540
     tattaggagc tgttggtgga cctaaatggg gtactggtga agttagacca gagcaaggtt       600
     tattaaagat tagaaaagaa ttaaatttat atgctaattt aaggccatgt aattttgcca       660
     gtgataaatt attagattta tcaccattaa aatctgatat tgttaaaggt acagatttta       720
     cagttgttag agaattagtt ggtggtattt attttggaga tagggttgaa gatgatggta       780
     gtggatttgc cagtgatagt gaaagttatt cagttccaga agttgaaagg atcactagaa       840
     tggcagcatt tttatcatta caaaatgatc caccattacc aatttggtca ttagataaag       900
     ccaatgtttt agcatctagt agattatgga ggaaaacagt tgatagagtt attaaagaag       960
     aatttccaaa attaacagtt caacatcaat taattgattc agcagcaatg atattagtta      1020
     aatcaccaac taaattaaat ggtattgtta ttacaaataa tatgtttgga gatattattt      1080
     ctgatgaagc atcagttatt ccaggatcat taggtttatt accatcagca tcattggcat      1140
     cattaccaga taccaatcaa gcatttggat tatatgagcc atgtcatgga tcagcacctg      1200
     atttaccaaa gaataaagtc aatccaattg caactatatt atcagcggca atgatgctta      1260
     aattatcatt aaatttagtt aaagaaggta atgcagttga agaagctgtt agaaaagtat      1320
     tagatcaagg tattatgact ggagatcttg gtggtaataa ttcaactact gaagttggtg      1380
     atgctattgc taaagaagtt aaattacttt tagcttaatt aatttaattt aattagatga      1440
     atttatataa gaattaaatt aaatttaata aaaataataa gaataatgaa tatacagtat      1500
     atatatatat atgaatacgt aataacatgt aataattgat aaatttgagg atagagtcta      1560
     gatcaattta ataatggtta caataatagt atatttggct ctgatcaggc gttgttcttt      1620
     ctttgtttct ttgtttcttt gtttctttgt ttctttgttt ctttgtttct ttgtttcttt      1680
     gtttgattgt ttgaaagctt                                                  1700
//
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