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EBI DbfetchID M98822; SV 1; linear; genomic DNA; STD; PRO; 4191 BP. XX AC M98822; XX DT 06-MAR-1993 (Rel. 35, Created) DT 17-APR-2005 (Rel. 83, Last updated, Version 9) XX DE Bacillus subtilis L-aspartate oxidase (nadB) and nifS genes, complete cds. XX KW L-aspartate oxidase; NAD biosynthesis; nadB gene; nifS gene. XX OS Bacillus subtilis OC Bacteria; Firmicutes; Bacillales; Bacillaceae; Bacillus. XX RN [1] RP 1-4191 RX PUBMED; 8444804. RA Sun D., Setlow P.; RT "Cloning, nucleotide sequence, and regulation of the Bacillus subtilis nadB RT gene and a nifS-like gene, both of which are essential for NAD RT biosynthesis"; RL J. Bacteriol. 175(5):1423-1432(1993). XX DR GOA; P39667. DR HSSP; 1J5Y; Q9X1T8. DR InterPro; IPR002638; Q_phspho_trans. DR UniProtKB/Swiss-Prot; P39666; NADC_BACSU. XX FH Key Location/Qualifiers FH FT source 1..4191 FT /organism="Bacillus subtilis" FT /strain="168" FT /mol_type="genomic DNA" FT /db_xref="taxon:1423" FT terminator 13..42 FT CDS complement(543..1730) FT /codon_start=1 FT /transl_table=11 FT /function="required for NAD biosynthesis" FT /note="homologous to the nifS gene products of other FT bacteria" FT /db_xref="GOA:P38033" FT /db_xref="HSSP:1P3W" FT /db_xref="InterPro:IPR015421" FT /db_xref="UniProtKB/Swiss-Prot:P38033" FT /protein_id="AAA21613.1" FT /translation="MIYLDYAATTPICEEALTVYQKLSMDMYGNASSLHDAGGKAKHIL FT EYCREKIANIIGGEASGIYFTSGGTESNFLAIQSLLNGLPKTKRHFITTAMEHQSIHNC FT AAFLEQQGYDVTVIEPNEYGLITEEILLTHIRPETGLVSIQHANSETGIIQPIQHLSSY FT LHNKGILLHCDAVQTFGKIPINTKNLGVDALSMSSHKIHGPKGVGAVYIRPDVPWKPVY FT PLTTHEYGFRAGTVNVPGIGAFTAAAELIVSEMEKQISRNEALRTYFLDQIRIRSLPVT FT LAADTSKAECLPHIIGCFFHSFEGQYVMLECNRSNICISTGSACSAGYHGPSETMKALR FT KTEQEALQFIRISFGRHTTAEQLEQLLHTFTVLWEQKKGEFDIDRRIKANGRQQA" FT RBS 1739..1743 FT /gene="nadB" FT -35_signal 1766..1771 FT /gene="nadB" FT -10_signal 1774..1779 FT /gene="nadB" FT -10_signal 1789..1794 FT /gene="nadB" FT -35_signal 1798..1803 FT /gene="nadB" FT RBS 1820..1825 FT /gene="nadB" FT CDS 1833..3428 FT /codon_start=1 FT /transl_table=11 FT /gene="nadB" FT /product="L-aspartate oxidase" FT /function="required for NAD biosynthesis" FT /db_xref="GOA:P38032" FT /db_xref="HSSP:1CHU" FT /db_xref="InterPro:IPR005288" FT /db_xref="UniProtKB/Swiss-Prot:P38032" FT /protein_id="AAA21614.1" FT /translation="MSKKTIAVIGSGAAALSLAAAFPPSYEVTVITKKSVKNSNSVYAQ FT GGIAAAYAKDDSIEAHLEDTLYAGCGHNNLAIVADVLHDGKMMVQSLLERGFPFDRNER FT GGVCLGREGAHSYNRIFHAGGDATGRLLIDYLLKRINSKIKLIENETAADLLIEDGRCI FT GVMTKDSKGRLKVRHADEVVLAAGGCGNLFLHHTNDLTVTGDGLSLAYRAGAELTDLEF FT TQFHPTLLVKNGVSYGLVSEAVRGEGGCLVDENGRRIMAERHPLGDLAPRDIVSRVIHE FT EMAKGNRVYIDFSAISDFETRFPTITAICEKAGIDIHSGKIPVAPGMHFLMGGVSVNRW FT GETTVPGLYAIGETACSGLHGANRLASNSLLEALVFGKRAAEHIIQKPVYNRQYQSGLE FT TSVFYEVPDIEGHELQSKMTSHMSILREQSSLIELSIWLHTLPFQEVNVKDITIRQMEL FT SHLWQTAKLMTFSALLREESRGAHFRTDFPHAEVSWQGRQIVHTKKGTKIRKNEGIWNN FT ESFTAEKITESLFS" XX SQ Sequence 4191 BP; 1125 A; 860 C; 1042 G; 1164 T; 0 other; tcttaccaat tataaaaaaa gcccactaga gggctttttt tagtctttaa ttaaaaatgc 60 cggcttcttc gagggcttgg caggcttgat cgatgcgatg ttcatcaggt gctgtcagtg 120 tatgcaggtg cacgccgtcg gtcagctgtg ataagtaggc agcattcgta gaattgattt 180 ttttgatgaa atggctaact tctttccggg tgcctacttg gatggctgca gttaaatctc 240 cgtatacggg atgctcgatt tttacgtctt ttactgtaac gccttcgtcg acgatgagct 300 gcagttcctc ttctgtccgt tcaggaccgt gcagacatgc tatgattctt tctgcctgct 360 ggtgctgctg agcggctgca tccatgtata catatccttg gcttgtggcg ataatcggta 420 catttttcgc tttcaagagc gatatatcct gtacaataac ctgtcttgag acgttcgctt 480 tttttgcgag ttctcctcct gtcagcggtg atttagattc cttcagccac agaagaagct 540 ggtcacgcct gttggcgccc attagcttta attcttcggt caatatcaaa ttctcctttc 600 ttttgttccc aaagcactgt aaacgtatgt aataactgtt ccagctgttc agctgttgta 660 tgcctgccaa aagagatgcg gataaattgc agagcttctt gttcggtttt tcttaatgct 720 ttcattgttt cagacggacc gtggtaacct gcagaacaag cgctgccggt tgaaatgcaa 780 atattactcc gattacattc taacataaca tattgtcctt caaatgagtg aaaaaaacac 840 cctataatat gcggaagaca ttctgctttc gaagtgtctg ctgcaagggt tacagggaga 900 gaccgaatac ggatttggtc tagaaagtat gttcttaatg cctcattgcg ggatatctgt 960 ttttccattt cgcttacgat caattctgca gcggcagtga aggcaccgat cccgggaaca 1020 tttacggtgc cggctctgaa tccgtattcg tgagtagtca gaggataaac gggtttccaa 1080 gggacatccg gccggatata gaccgcccct actcctttag gaccgtggat tttatggctg 1140 gacattgaga gtgcatctac tcccagattt tttgtattga tcgggatctt tccaaacgtt 1200 tgaacagcgt cgcaatgaag aagaatcccc ttattgtgca ggtatgaaga caggtgttga 1260 atgggctgaa tgatcccggt ttcagaattt gcatgctgaa tagatacaag gcctgtttcc 1320 ggccggatat gggttagtaa tatttcctcg gtaataagcc cgtattcatt cggttcaatg 1380 actgttacat catatccctg ctgttccaaa aaagcagcgc aattatgaat ggactgatgc 1440 tccatggcag tcgtaataaa atgtcttttc gtttttggca ggccgtttaa caatgattga 1500 atggccagaa aattggattc agtgccgccg cttgtaaaat agatgccgct tgcttctccg 1560 ccaatgatgt tggcgatttt ttcacggcag tactccagta tgtgctttgc ttttcctcct 1620 gcatcatgca ggctgctggc atttccgtac atatccatac taagcttctg atatacagta 1680 agggcttcct cacaaatagg cgttgtcgct gcataatcta aataaatcat ccggtcttcc 1740 tccatccgtt ctccataaaa aactcttgag tttattttat ccttgtgtaa atataggtgt 1800 caagacaggt gtaaacaaca ggaggatggc atatgtctaa aaagacgatt gcagtcatcg 1860 gttcaggggc ggcggcactt tccttagctg cagctttccc gccctcatac gaagtgactg 1920 ttatcacaaa aaaaagcgtc aaaaacagca attctgtcta tgcacaaggc gggattgctg 1980 cggcctatgc aaaagatgac tcgattgaag cccatctgga ggatacgtta tacgcagggt 2040 gcggccataa taatctagct attgtagcag acgtattaca tgatggaaaa atgatggttc 2100 aaagcctatt ggagcgtgga tttccattcg accgcaatga acgaggcggt gtttgtcttg 2160 gaagagaagg ggcccactca tacaaccgca tatttcatgc aggcggagac gcaacaggca 2220 ggctgcttat cgattatttg ctcaaacgga tcaacagcaa aatcaagtta atcgagaatg 2280 aaacggcagc agatttgctt atagaggacg gacgatgtat cggcgtcatg acaaaagaca 2340 gcaaagggcg gctcaaggta aggcacgcag acgaagttgt gctggctgcc ggcggctgtg 2400 gaaacctgtt tcttcaccat acgaatgatc tcactgtcac aggtgacggt ctttctctgg 2460 cttaccgggc cggggcggaa ttaacggatt tggagtttac tcagtttcac ccgacactgc 2520 ttgtgaaaaa tggtgtttcc tacgggcttg tttcagaagc tgtcagaggg gaaggcggat 2580 gtttagtaga cgaaaacggc cgcaggatta tggctgaacg gcatccttta ggtgacctgg 2640 ctccaagaga tattgtctca cgggttattc acgaagaaat ggcaaaagga aatcgcgttt 2700 atatagactt cagtgcgatt tctgattttg aaacgcgttt ccctaccatc accgctattt 2760 gtgaaaaagc agggattgat atccacagcg gaaaaattcc tgttgctccg ggaatgcatt 2820 tcttaatggg aggtgtttca gttaatcgct ggggagaaac gacagtgccg gggctttatg 2880 cgattggcga gacagcatgc tccggtttac atggagcaaa ccggcttgca agcaattctt 2940 tattggaagc gctggtattt ggaaaaaggg cagcagagca tattatccaa aaaccggttt 3000 ataacaggca atatcaatca gggctagaaa ccagtgtctt ctacgaggtg ccggatatag 3060 aggggcatga actgcaaagc aaaatgacaa gccacatgtc tattctgcgg gaacaaagca 3120 gtttgattga gcttagcatc tggcttcata cgctgccttt tcaggaagta aatgtgaagg 3180 atatcacaat tcggcagatg gaactttctc atctatggca aactgcaaag ctgatgacgt 3240 tttctgcgct tttgcgggag gaaagcagag gggctcactt ccgcaccgat tttcctcatg 3300 ctgaggtgag ctggcaagga agacaaattg tccatacaaa aaaaggaacg aagatcagaa 3360 aaaacgaggg gatttggaac aatgaatcat ttacagctga aaaaattact gaatcacttt 3420 tttcttgagg atattgggac aggggatctt acatcgcagt ctatttttgg agaacagagc 3480 tgtgaagcgg agattgttgc aaagtcagag ggtatttttg caggagcagc gatcataaaa 3540 gaaggctttt cgttattgga cgaaaatgtt caaagcattt tacacaaaaa agatggggac 3600 atgttgcata aaggtgaagt gattgccgag ttacacggtc ccgcagctgc gctcctttca 3660 ggagagcggg tcgtattaaa tcttattcag aggctgtcag gaattgcgac aatgacaaga 3720 gaggctgttc ggtgtctgga tgatgaacag attaaaattt gtgacacgag aaaaacaaca 3780 ccggggctca gaatgctgga gaaatatgct gtcagagccg gcggcgggta taatcatcga 3840 ttcggtttgt atgacggcat catgattaaa gataatcata tcgcagcatg cggctctatt 3900 ttagaggcgt gcaaaaaagc ccgtcaggcc gcagggcaca tggtaaatat cgaggtggaa 3960 attgaaacgg aggaacagct gcgggaagca attgcagctg gagcggatgt tattatgttt 4020 gacaactgtc cgcctgatac ggtccgtcat tttgcaaagc tcacaccggc taatataaaa 4080 actgaagcct ctggcgggat tacgttagaa tctttgcctg cttttaaagg gacgggtgtg 4140 aattatattt cattaggatt tctgactcat tccgtcaaaa gtttagatat c 4191 // |
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