EBI Dbfetch

ID   M98806; SV 1; linear; genomic DNA; STD; PRO; 2191 BP.
XX
AC   M98806;
XX
DT   15-AUG-1992 (Rel. 33, Created)
DT   04-MAR-2000 (Rel. 63, Last updated, Version 4)
XX
DE   Pseudomonas pickettii phenol/cresol hydroxylase (tbuD) gene, complete cds.
XX
KW   phenol hydroxylase; tbuD gene.
XX
OS   Ralstonia pickettii
OC   Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
OC   Burkholderiaceae; Ralstonia.
XX
RN   [1]
RP   1-2191
RX   PUBMED; 1400204.
RA   Kukor J.J., Olsen R.H.;
RT   "Complete nucleotide sequence of tbuD, the gene encoding phenol/cresol
RT   hydroxylase from Pseudomonas pickettii PKO1, and functional analysis of the
RT   encoded enzyme";
RL   J. Bacteriol. 174(20):6518-6526(1992).
XX
FH   Key             Location/Qualifiers
FH
FT   source          1..2191
FT                   /organism="Ralstonia pickettii"
FT                   /strain="PKO1"
FT                   /mol_type="genomic DNA"
FT                   /db_xref="taxon:329"
FT   promoter        19..45
FT                   /gene="tbuD"
FT   RBS             70..73
FT                   /gene="tbuD"
FT   CDS             83..2098
FT                   /codon_start=1
FT                   /transl_table=11
FT                   /gene="tbuD"
FT                   /product="phenol hydroxylase"
FT                   /db_xref="GOA:Q01551"
FT                   /db_xref="InterPro:IPR003042"
FT                   /db_xref="UniProtKB/Swiss-Prot:Q01551"
FT                   /protein_id="AAA25992.1"
FT                   /translation="MTKYNEAYCDVLIVGAGPAGVMAAAHLLSYGTTARPHRVRIFDAT
FT                   KEVNGSDESTESLSTDVIADALNSGASGPEKDAASTTEDLPMLVTTLQVSDVLHDTGDD
FT                   TKIAYRETATEQQVLLLADTTANTSSTMNPRSMCEAGCRFHQIYQGHCFPEYELDSERL
FT                   RSVDGRAQVLEDEHETGQLRLERLGRPEELLELDEENSMSVVTNLKAAPYKFLMKDVDE
FT                   NFPGELSTSGGKTTSISADESAIDAALHAVWDADDLGAAWHLDEASGLRAVDWNAAQWF
FT                   KSGQPWTPDAAKSLQEGRVFLAGDARHRHPPLTGIGKNTSIADCYNLTWKLLGVLLGVA
FT                   RADPARTYVAERVYIRMRAATDIAVDAEMESLAAKWITVQLTLSRSWISSAKEAERWDA
FT                   VLRDSAMSASKPMWTTSDMRASFDAGLMGHGHAHDHVTPTIKEFASSSISRSISELAST
FT                   SWWESRGWGNGGPFESLMEDARWTGAVESNCRYAAYDRDAPVLHEHVAWVTRFTSRART
FT                   AVLEAAVGQAHVVDCWDVGLVEPALDDLDSAGAGLHVAHHADQWPAQLDEAVWPRESLS
FT                   DWRIVTDTSATGEGYQTSPREAPGDYADLNADNAKAHFNGQFAGHKAYGDAAAADGGGC
FT                   HGRILVGPAVRGRHLHREIPLGEECQRAAQPLFKEV"
FT   mat_peptide     86..2095
FT                   /gene="tbuD"
FT                   /product="phenol hydroxylase"
FT                   /EC_number="1.14.13.7"
FT                   /experiment="experimental evidence, no additional details
FT                   recorded"
FT   terminator      2141..2172
XX
SQ   Sequence 2191 BP; 461 A; 626 C; 708 G; 396 T; 0 other;
     aggcctggga gatgtaccgc gatggcaagc gcggtgcttg ctacagtgga gggcaacggc        60
     ggcgcgctgg aggtggcgcg ctatgacgaa gtacaacgag gcgtactgcg acgtactcat       120
     cgttggggcg ggacccgccg gagtaatggc cgccgcgcat ctgctatctt atggaactac       180
     ggcgcggccg caccgtgtac gtatcttcga cgcgacgaag gaagtcaatg gctctgacga       240
     aagtaccgag agtctctcga cagatgttat cgccgacgct ttgaattctg gcgcgagcgg       300
     gccggagaag gacgccgctt ctacaacaga ggatctgccg atgctggtca cgaccctgca       360
     agtgtccgat gttcttcacg acaccgggga cgacaccaag atcgcctatc gcgagacagc       420
     tactgagcaa caagtactcc ttcttgctga caccactgca aacacatctt cgacgatgaa       480
     cccgagaagt atgtgcgaag ctggctgccg gtttcaccag atctatcagg gccattgctt       540
     cccggagtac gagctcgaca gcgagaggct tcgatccgtt gatggccgtg ctcaagtact       600
     agaggatgaa catgagacgg gacaacttcg acttgagagg ctcggaagac cagaagaact       660
     tctggagctg gacgaggaaa acagcatgag cgtcgtgacc aacctgaaag ccgcgcccta       720
     caagtttctg atgaaggatg tggacgagaa ctttcccggc gagctgtcta catcgggtgg       780
     gaagaccact tcaatttctg cggacgagtc tgccatcgac gccgccctac atgccgtttg       840
     ggacgctgat gacctcggtg ctgcctggca tctagacgag gcatccggac ttcgagcggt       900
     cgactggaat gcggcgcagt ggttcaagtc cggtcagccc tggacgcccg atgcggccaa       960
     gtcgctgcaa gagggccgtg tgttcctcgc gggcgacgcc cgtcatcgcc acccgcccct      1020
     caccggcatc ggtaagaaca ccagtatagc cgattgctat aacctcacct ggaagctcct      1080
     cggcgtcctg ctgggcgtgg cgagggcaga ccctgctcga acctacgttg ccgagcgggt      1140
     gtacatccgc atgcgtgcgg ccacggacat tgcggtagat gcagagatgg agtcactcgc      1200
     ggcgaagtgg atcacggtgc agctcaccct ctcgcgctca tggatatcga gcgcgaagga      1260
     ggcagaacgc tgggatgctg tgctccggga ctcagcgatg tctgcatcga agccgatgtg      1320
     gacgacgagc gacatgcgcg cgtcattcga tgcgggactt atgggccacg gtcacgcgca      1380
     tgaccacgtc acgcccacca tcaaggagtt cgcctcaagc tcgatcagcc gatcgatttc      1440
     cgagctggcc agtacgtcat ggtgggaatc ccggggctgg ggcaacggcg ggccttttga      1500
     gtcgctcatg gaggacgcga ggtggaccgg tgcggtggaa tcgaactgca ggtacgccgc      1560
     gtacgaccgc gacgcaccgg tgctgcacga gcacgttgcg tgggtgacac gcttcacgtc      1620
     acgggcccgt acggcggttc ttgaggcggc agtcggccaa gcccatgttg ttgattgctg      1680
     ggacgtcggg ctcgtcgagc ccgcgctcga tgatctcgac tctgctggag cagggctgca      1740
     cgttgcccat cacgctgatc aatggccagc gcagctggac gaagctgtat ggccacgaga      1800
     gagtttgtcg gactggcgga tcgtcaccga cacttcagct acgggtgagg gttatcaaac      1860
     gagcccgagg gaagcgcctg gagactacgc ggaccttaac gcagacaacg ccaaggcgca      1920
     tttcaacggg cagtttgcgg ggcacaaggc gtatggggac gctgccgccg ccgatggtgg      1980
     aggctgccat gggcgcattc ttgtagggcc ggctgttcga ggacgacatc tacaccgaga      2040
     aattcctctc ggcgaggagt gccaacgcgc agcgcagccg ctgttcaagg aggtttgaaa      2100
     ggagacccac gcgatgctga tacccgaccc aaggtggacg tatgcgtgaa cagacccagg      2160
     agtcactgca tagcgcgacc accgaaagct t                                     2191
//