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EBI DbfetchID M97391; SV 1; linear; genomic DNA; STD; PRO; 3698 BP. XX AC M97391; M96937; XX DT 06-MAR-1993 (Rel. 35, Created) DT 17-APR-2005 (Rel. 83, Last updated, Version 7) XX DE Bacillus subtilis branched chain alpha-keto acid dehydrogenase E1-alpha, DE branched chain alpha-keto acid dehydrogenase E1-beta, and branched chain DE alpha-keto acid dehydrogenase E2, complete cds. XX KW branched chain alpha-keto acid dehydrogenase. XX OS Bacillus subtilis OC Bacteria; Firmicutes; Bacillales; Bacillaceae; Bacillus. XX RN [1] RP 1-3698 RX DOI; 10.1111/j.1432-1033.1993.tb17858.x RX PUBMED; 8504804. RA Wang G.F., Kuriki T., Roy K.L., Kaneda T.; RT "The primary structure of branched-chain alpha-oxo acid dehydrogenase from RT Bacillus subtilis and its similarity to other alpha-oxo acid RT dehydrogenases"; RL Eur. J. Biochem. 213(3):1091-1099(1993). XX DR GOA; P54533. DR InterPro; IPR000815; Hg_reductase. DR InterPro; IPR001327; Pyr_OxRdtase_NAD_bd. DR InterPro; IPR004099; Pyr_nucl-diS_OxRdtase_dimer. DR InterPro; IPR006258; Lipoamide_DH. DR InterPro; IPR012999; Pyr_OxRdtase_I_AS. DR InterPro; IPR013027; FAD_pyr_nucl-diS_OxRdtase. DR InterPro; IPR016156; FAD/NAD-linked_Rdtase_dimer. DR UniProtKB/Swiss-Prot; P54533; DLDH2_BACSU. XX FH Key Location/Qualifiers FH FT source 1..3698 FT /organism="Bacillus subtilis" FT /mol_type="genomic DNA" FT /db_xref="taxon:1423" FT RBS 204..218 FT CDS 216..1208 FT /codon_start=1 FT /transl_table=11 FT /product="branched chain alpha-keto acid dehydrogenase FT E1-alpha" FT /db_xref="GOA:P37940" FT /db_xref="InterPro:IPR001017" FT /db_xref="UniProtKB/Swiss-Prot:P37940" FT /protein_id="AAA22278.1" FT /translation="MSTNRHQALGLTDQEAVDMYRTMLLARKIDERMWLLNRSGKIPFV FT ISCQGQEAAQVGAAFALDREMDYVLPYYRDMGVVLAFGMTAKDLMMSGFAKAADPNSGG FT RQMPGHFGQKKNRIVTGSSPVTTQVPHAVGIALAGRMEKKDIAAFVTFGEGSSNQGDFH FT EGANFAAVHKLPVIFMCENNKYAISVPYDKQVACENISDRAIGYGMPGVTVNGNDPLEV FT YQAVKEARERARRGEGPTLIETISYRLTPHSSDDDDSSYRGREEVEEAKKSDPLLTYQA FT YLKETGLLSDEIEQTMLDEIMAIVNEATDEAENAPYAAPESALDYVYAK" FT RBS 1209..1224 FT CDS 1222..2205 FT /codon_start=1 FT /transl_table=11 FT /product="branched chain alpha-keto acid dehydrogenase FT E1-beta" FT /db_xref="GOA:P37941" FT /db_xref="InterPro:IPR005475" FT /db_xref="InterPro:IPR005476" FT /db_xref="InterPro:IPR009014" FT /db_xref="InterPro:IPR015941" FT /db_xref="UniProtKB/Swiss-Prot:P37941" FT /protein_id="AAA22279.1" FT /translation="MSVMSYIDAINLAMKEEMERDSRVFVLGEDVGRKGGVFKATAGLY FT EQFGEERVMDTPLAESAIAGVGIGAAMYGMRPIAEMQFADFIMPAVNQIISEAAKIRYR FT SNNDWSCPIVVRAPYGGGVHGALYHSQSVEAIFANQPGLKIVMPSTPYDAKGLLKAAVR FT DEDPVLFFEHKRAYRLIKGEVPADDYVLPIGKADVKREGDDITVITYGLCVHFALQAAE FT RLEKDGISAHVVDLRTVYPLDKEAIIEAASKTGKVLLVTEDTKEGSIMSEVAAIISEHC FT LFDLDAPIKRLAGPDIPAMPYAPTMEKYFMVNPDKVEAAMRELAEF" FT RBS 2215..2230 FT CDS 2228..3502 FT /codon_start=1 FT /transl_table=11 FT /product="branched chain alpha-keto acid dehydrogenase E2" FT /db_xref="GOA:P37942" FT /db_xref="InterPro:IPR000089" FT /db_xref="InterPro:IPR001078" FT /db_xref="InterPro:IPR003016" FT /db_xref="InterPro:IPR004167" FT /db_xref="InterPro:IPR011053" FT /db_xref="UniProtKB/Swiss-Prot:P37942" FT /protein_id="AAA22280.1" FT /translation="MAIEQMTMPQLGESVTEGTISKWLVAPGDKVNKYDPIAEVMTDKV FT NAEVPSSFTGTITELVGEEGQTLQVGEMICKIETEGANPAEQKQEQPAASEAAENPVAK FT SAGAADQPNKKRYSPAVLRLAGEHGIDLDQVTGTGAGGRITRKDIQRLIETGGVQEQNP FT EELKTAAPAPKSASKPEPKEETSYPASAAGDKEIPVTGVRKAIASNMKRSKTEIPHAWT FT MMEVDVTNMVAYRNSIKDSFKKTEGFNLTFFAFFVKAVAQALKEFPQMNSMWAGDKIIQ FT KKDINISIAVATEDSLFVPVIKNADEKTIKGIAKDITGLAKKVRDGKLTADDMQGGTFT FT VNNTGSFGSVQSMGIINYPQAAILQVESIVKRPVVMDNGMIAVRDMVNLCLSLDHRVLD FT GLVCGRFLGRVKQILESIDEKTSVY" FT terminator 3509..3553 XX SQ Sequence 3698 BP; 1116 A; 779 C; 950 G; 853 T; 0 other; agatacagat gatattctcg gcgttcatat gattggcccg catgtgcacc gacatgattt 60 ctgaagcggg tcttgccaaa gtgctggacg caacaccgtg ggaggtcggg caaacgattc 120 acccgcatcc aacgctttct gaagcaattg gagaagctgc gcttgccgca gatggcaaag 180 ccattcattt ttaaaagcat aaaggagggg cttgaatgag tacaaaccga catcaagcac 240 tagggctgac tgatcaggaa gccgttgata tgtatagaac catgctgtta gcaagaaaaa 300 tcgatgaaag aatgtggctg ttaaaccgtt ctggcaaaat tccatttgta atctcttgtc 360 aaggacagga agcagcacag gtaggagcgg ctttcgcact tgaccgtgaa atggattatg 420 tattgccgta ctacagagac atgggtgtcg tgctcgcgtt tggcatgaca gcaaaggact 480 taatgatgtc cgggtttgca aaagcagcag atccgaactc aggaggccgc cagatgccgg 540 gacatttcgg acaaaagaaa aaccgcattg tgacgggatc atctccggtt acaacgcaag 600 tgccgcacgc agtcggtatt gcgcttgcgg gacgtatgga gaaaaaggat atcgcagcct 660 ttgttacatt cggggaaggg tcttcaaacc aaggcgattt ccatgaaggg gcaaactttg 720 ccgctgtcca taagctgccg gttattttca tgtgtgaaaa caacaaatac gcaatctcag 780 tgccttacga taagcaagtc gcatgtgaga acatttccga ccgtgccata ggctatggga 840 tgcctggcgt aactgtgaat ggaaatgatc cgctggaagt ttatcaagcg gttaaagaag 900 cacgcgaaag ggcacgcaga ggagaaggcc cgacattaat tgaaacgatt tcttaccgcc 960 ttacaccaca ttccagtgat gacgatgaca gcagctacag aggccgtgaa gaagtagagg 1020 aagcgaaaaa aagtgatccc ctgcttactt atcaagctta cttaaaggaa acaggcctgc 1080 tgtccgatga gatagaacaa accatgctgg atgaaattat ggcaatcgta aatgaagcga 1140 cggatgaagc ggagaacgcc ccatatgcag ctcctgagtc agcgcttgat tatgtttatg 1200 cgaagtaggg aggaagaaca aatgtcagta atgtcatata ttgatgcaat caatttggcg 1260 atgaaagaag aaatggaacg agattctcgc gttttcgtcc ttggggaaga tgtaggaaga 1320 aaaggcggtg tgtttaaagc gacagcggga ctctatgaac aatttgggga agagcgcgtt 1380 atggatacgc cgcttgctga atctgcaatc gcaggagtcg gtatcggagc ggcaatgtac 1440 ggaatgagac cgattgctga aatgcagttt gctgatttca ttatgccggc agtcaaccaa 1500 attatttctg aagcggctaa aatccgctac cgcagcaaca atgactggag ctgtccgatt 1560 gtcgtcagag cgccatacgg cggaggcgtg cacggagccc tgtatcattc tcaatcagtc 1620 gaagcaattt tcgccaacca gcccggactg aaaattgtca tgccatcaac accatatgac 1680 gcgaaagggc tcttaaaagc cgcagttcgt gacgaagacc ccgtgctgtt ttttgagcac 1740 aagcgggcat accgtctgat aaagggcgag gttccggctg atgattatgt cctgccaatc 1800 ggcaaggcgg acgtaaaaag ggaaggcgac gacatcacag tgatcacata cggcctgtgt 1860 gtccacttcg ccttacaagc tgcagaacgt ctcgaaaaag atggcatttc agcgcatgtg 1920 gtggatttaa gaacagttta cccgcttgat aaagaagcca tcatcgaagc tgcgtccaaa 1980 actggaaagg ttcttttggt cacagaagat acaaaagaag gcagcatcat gagcgaagta 2040 gccgcaatta tatccgagca ttgtctgttc gacttagacg cgccgatcaa acggcttgca 2100 ggtcctgata ttccggctat gccttatgcg ccgacaatgg aaaaatactt tatggtcaac 2160 cctgataaag tggaagcggc gatgagagaa ttagcggagt tttaaagacg taagggagga 2220 tacaatcatg gcaattgaac aaatgacgat gccgcagctt ggagaaagcg taacagaggg 2280 gacgatcagc aaatggcttg tcgcccccgg tgataaagtg aacaaatacg atccgatcgc 2340 ggaagtcatg acagataagg taaatgcaga ggttccgtct tcttttactg gtacgataac 2400 agagcttgtg ggagaagaag gccaaaccct gcaagtcgga gaaatgattt gcaaaattga 2460 aacagaaggc gcgaatccgg ctgaacaaaa acaagaacag ccagcagcat cagaagccgc 2520 tgagaaccct gttgcaaaaa gtgctggagc agccgatcag cccaataaaa agcgctactc 2580 gccagctgtt ctccgtttgg ccggagagca cggcattgac ctcgatcaag tgacaggaac 2640 tggtgccggc gggcgcatca cacgaaaaga tattcagcgc ttaattgaaa caggcggcgt 2700 gcaagaacag aatcctgagg agctgaaaac agcagctcct gcaccgaagt ctgcatcaaa 2760 acctgagcca aaagaagaga cgtcatatcc tgcgtctgca gccggtgata aagaaatccc 2820 tgtcacaggt gtaagaaaag caattgcttc caatatgaag cgaagcaaaa cagaaattcc 2880 gcatgcttgg acgatgatgg aagtcgacgt cacaaatatg gttgcatatc gcaacagtat 2940 aaaagattct tttaagaaga cagaaggctt taatttaacg ttcttcgcct tttttgtaaa 3000 agcggtcgct caggcgttaa aagaattccc gcaaatgaat agcatgtggg cgggggacaa 3060 aattattcag aaaaaggata tcaatatttc aattgcagtt gccacagagg attctttatt 3120 tgttccggtg attaaaaacg ctgatgaaaa aacaattaaa ggcattgcga aagacattac 3180 cggcctagct aaaaaagtaa gagacggaaa actcactgca gatgacatgc agggaggcac 3240 gtttaccgtc aacaacacag gttcgttcgg gtctgttcag tcgatgggca ttatcaacta 3300 ccctcaggct gcgattcttc aagtagaatc catcgtcaaa cgcccggttg tcatggacaa 3360 tggcatgatt gctgtcagag acatggttaa tctgtgcctg tcattagatc acagagtgct 3420 tgacggtctc gtgtgcggac gattcctcgg acgagtgaaa caaattttag aatcgattga 3480 cgagaagaca tctgtttact aaataagcaa aaagagcatt ttttgaagtt ttgtttcaaa 3540 aaatgctctt tttctatgct ttattattca gcgatccgta ttttcatttc gactcgatat 3600 tcttcttgtt ttttcgggga gtaatgaatc ggtatgatta actcgtatac atcactgaca 3660 actgttaatt ggcggtccgc gatatatttg ataagctt 3698 // |
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