EBI Dbfetch

ID   M92297; SV 2; linear; genomic DNA; STD; PRO; 1881 BP.
XX
AC   M92297;
XX
DT   28-APR-1992 (Rel. 31, Created)
DT   17-APR-2005 (Rel. 83, Last updated, Version 5)
XX
DE   Streptomyces lividans mannanase (manA) gene, complete cds.
XX
KW   .
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OS   Streptomyces lividans
OC   Bacteria; Actinobacteria; Actinobacteridae; Actinomycetales;
OC   Streptomycineae; Streptomycetaceae; Streptomyces.
XX
RN   [1]
RP   1-1881
RX   PUBMED; 8457214.
RA   Arcand N., Kluepfel D., Paradis F.W., Morosoli R., Shareck F.;
RT   "Beta-mannanase of Streptomyces lividans 66: cloning and DNA sequence of
RT   the manA gene and characterization of the enzyme";
RL   Biochem. J. 290:857-863(1993).
XX
RN   [2]
RP   1-1881
RA   Shareck F.;
RT   ;
RL   Submitted (28-APR-1992) to the EMBL/GenBank/DDBJ databases.
RL   Centre de recherche en microbiologie appliquee, Institut Armand-Frappier,
RL   531 boul. des Prairies, Laval, Quebec H7N 4Z3, Canada
XX
RN   [3]
RC   Sequence update by submitter
RP   1-1881
RA   Shareck F.;
RT   ;
RL   Submitted (08-APR-1999) to the EMBL/GenBank/DDBJ databases.
RL   Centre de recherche en microbiologie appliquee, Institut Armand-Frappier,
RL   531 boul. des Prairies, Laval, Quebec H7N 4Z3, Canada
XX
CC   On Apr 8, 1999 this sequence version replaced gi:153193.
XX
FH   Key             Location/Qualifiers
FH
FT   source          1..1881
FT                   /organism="Streptomyces lividans"
FT                   /strain="1326"
FT                   /mol_type="genomic DNA"
FT                   /db_xref="taxon:1916"
FT   RBS             217..221
FT                   /gene="manA"
FT                   /note="putative"
FT   CDS             226..1377
FT                   /codon_start=1
FT                   /transl_table=11
FT                   /gene="manA"
FT                   /product="mannanase"
FT                   /EC_number="3.2.1.78"
FT                   /db_xref="GOA:P51529"
FT                   /db_xref="HSSP:1E8R"
FT                   /db_xref="InterPro:IPR013781"
FT                   /db_xref="UniProtKB/Swiss-Prot:P51529"
FT                   /protein_id="AAA26710.2"
FT                   /translation="MRNARSTLITTAGMAFAVLGLLFALAGPSAGRAEAAAGGIHVSNG
FT                   RVVEGNGSAFVMRGVNHAYTWYPDRTGSIADIAAKGANTVRVVLSSGGRWTKTSASEVS
FT                   ALIGQCKANKVICVLEVHDTTGYGKDGATSLDQAGDYWVGVKSAAWRAQEDYVVVNIGN
FT                   EPFGNTNYAAWTDATKSAIGKLRGAGLGHALMVDAPNWGQDWSGTMRSNAASVFASDPD
FT                   RNTVFSIHMYGVYDTAAEVRDYLNAFVGNGLPIVVGEFGDQHSDGNPDEDAIMATAQSL
FT                   GVGYLGWSWSGNGGGVEYLDMVNGFDPNSLTSWGNRILYGSNGIAATSRTATVYGGGGG
FT                   STGGTAPNGYPYCVNGGASDPDGDGWGWENSRSCVVRGSAADH"
FT   sig_peptide     226..333
FT                   /gene="manA"
FT   mat_peptide     334..1374
FT                   /product="mannanase"
FT                   /function="hydrolase"
FT                   /EC_number="3.2.1.78"
XX
SQ   Sequence 1881 BP; 309 A; 648 C; 647 G; 277 T; 0 other;
     caatactacg tgggcgcccc ctctttttac taaacccgta aacgcgctga ccagagtgtg        60
     aagcccgcgt atccaggtcg ttcacctgcg tcaactgccc ctgcatgacg gattgacagg       120
     cctgccaggt gtccctacgg tccctatcgg ttaaccggta aatgtcgccc cgaggtgcgg       180
     gataacgttg tcaggtgcat gctcgcccgc catcgaagga gacacatgcg taacgcaaga       240
     agcacgctga tcacgaccgc ggggatggcg ttcgccgttc tgggcctgct gttcgcactc       300
     gccggtcctt ccgccggaag ggcggaggcc gcggccggtg gcatccacgt cagcaacgga       360
     cgtgtcgtcg agggcaacgg cagcgcgttc gtcatgcgcg gggtcaacca cgcctacacc       420
     tggtatcccg accgcaccgg ctccatcgcc gacatcgcgg ccaagggagc caacaccgtc       480
     cgcgtcgtgc tcagcagcgg cggccgctgg acgaagacga gcgcgtccga ggtctcggcg       540
     ctcatcggcc agtgcaaggc caacaaggtc atctgtgtcc ttgaggtgca cgacaccacc       600
     ggctacggca aggacggcgc gaccagcctg gaccaggccg gcgactactg ggtcggtgtg       660
     aagagcgccg cctggagggc ccaggaggac tacgtcgtcg tcaacatcgg caacgaaccc       720
     ttcggcaaca ccaactacgc cgcgtggacc gacgcgacca agtcggccat cggcaagctc       780
     cgcggcgccg gtctcggcca cgccctgatg gtcgacgcgc ccaactgggg ccaggactgg       840
     tccggcacga tgcggtccaa cgcggcctcg gtcttcgcct ccgaccccga ccgcaacacc       900
     gtcttctcca tccacatgta cggggtctac gacaccgccg ccgaggtgcg ggactacctg       960
     aacgccttcg tcggcaacgg actgcccatc gtcgtcggcg agttcggcga ccagcacagt      1020
     gacggcaacc ccgacgagga cgccatcatg gcgacggccc agtccctcgg cgtcggctac      1080
     ctcggctggt cctggagcgg caacggcggc ggcgtcgagt acctcgacat ggtcaacggc      1140
     ttcgacccga actcgctgac gagctggggc aaccgcatcc tctacggcag caacggcatc      1200
     gccgcgacgt ccaggacggc caccgtgtac ggcggtggtg gcggcagcac gggcggcacc      1260
     gcgcccaacg gttacccgta ctgcgtgaac ggcggtgcgt ccgacccgga cggtgacggg      1320
     tggggctggg agaacagccg gtcctgcgtc gtccggggca gcgcggcgga ccactgaccc      1380
     gaccgacggc gacgccccac cctggggttc cggggtccgg ggccgtttcc agtgattcgg      1440
     gcccacgccg tggggagacg gcgagctggt gccgtgcggc gccttcgagt cgcggatgtg      1500
     gatcgcgtcc gggcgggccg ccacctcgac acagtcgccg tcaccgctgc tgctgtagct      1560
     cgacttgtgc caggacaggg cgatttccac gcagtcgccg tcgccaccgc tgctgtacga      1620
     gcttttgaac caggccagtt ccgtcgtgtt catgaggctc ctcgcatccg ccgcaacagg      1680
     cccagggagt cgtcgaggga gaggggcctg tgatctcgtc gaaggcggcg ggggcgccgg      1740
     acgcggccgg gttcccgggg acctcggggg tgtggtgccc ccgatcgtcc gcgcgggcgg      1800
     cgggaagggc gaccagcggg acaccggcgg cgacgacggc tgcggcgccg agcgcgcgac      1860
     gacgggtgag ttccggcatg c                                                1881
//