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EBI DbfetchID M91609; SV 1; linear; genomic DNA; STD; UNC; 5516 BP. XX AC M91609; XX DT 07-JAN-1993 (Rel. 34, Created) DT 15-JAN-2007 (Rel. 90, Last updated, Version 7) XX DE Plasmid pTiS4 (from Agrobacterium vitis) T2 tryptophan monooxygenase (iaaM) DE and indole acetamide hydrolase (iaaH) genes, complete cds. XX KW iaaH gene; iaaM gene; indole-3-acetamide hydrolase; KW tryptophan monooxygenase. XX OS Plasmid pTiS4 OC other sequences; plasmids. OG Plasmid pTiS4 XX RN [1] RP 1-5516 RX DOI; 10.1007/BF00279373 RX PUBMED; 1465104. RA Canaday J., Gerad J.C., Crouzet P., Otten L.; RT "Organization and functional analysis of three T-DNAs from the vitopine Ti RT plasmid pTiS4"; RL Mol. Gen. Genet. 235(2-3):292-303(1992). XX FH Key Location/Qualifiers FH FT source 1..5516 FT /organism="Plasmid pTiS4" FT /plasmid="pTiS4" FT /host="Agrobacterium vitis" FT /mol_type="genomic DNA" FT /db_xref="taxon:2652" FT misc_signal 357..364 FT /note="Chi sequence" FT gene complement(676..2281) FT /gene="iaaH" FT polyA_signal complement(676..680) FT /gene="iaaH" FT CDS complement(854..2242) FT /codon_start=1 FT /transl_table=11 FT /gene="iaaH" FT /product="indole acetamide hydrolase" FT /function="auxin synthesis; root formation on host plants" FT /db_xref="GOA:Q04557" FT /db_xref="InterPro:IPR000120" FT /db_xref="InterPro:IPR020556" FT /db_xref="UniProtKB/Swiss-Prot:Q04557" FT /protein_id="AAA98148.1" FT /translation="MVEISSISQTLRALRRKQYSCRDLVESLVSRSESATHLNAFAATD FT WLHLRNEADRVDRNGSGGVGLMGIPLCFKANIATGIFPTSAGTQGLLRHKPAIPAKIVE FT RLHSAGALIGASGNMHELSFGITNDNKTFGPARNPWNQALISGGSSGGVAVSVAANLML FT GGIGTDTGASVRLPAALCGVVGFRPTFGNYPTDGIVPVSPSRDTPGLIVRSVEDAVLLD FT RIIRNKCPTQNMSLKGLRLGLPRSHFFDNLEPHVAAASERAIRRLATNQMTFVEADIPN FT VAELTRKVSLPVAIYEFPRALMAYLSFHGIGNTFDELIQNIQDPQVYDLVQSQLSKGLI FT SESVYRRALRCYKPRLKATYENYYSSNGLDAVLFPSVPLTAKPVGLQTTLVHNGVETDT FT FGIFVRNLDPSSNIGLPSLSVPVSLTPDRLPVGIQIEGPFGSDDMVLAIGRAVEEMVKF FT GQEY" FT TATA_signal complement(2276..2281) FT /gene="iaaH" FT gene complement(2573..4822) FT /gene="iaaM" FT CDS 2573..4744 FT /codon_start=1 FT /transl_table=11 FT /gene="iaaM" FT /product="tryptophan monooxygenase" FT /db_xref="GOA:Q04564" FT /db_xref="InterPro:IPR000759" FT /db_xref="InterPro:IPR001878" FT /db_xref="InterPro:IPR002937" FT /db_xref="InterPro:IPR006064" FT /db_xref="InterPro:IPR012142" FT /db_xref="UniProtKB/Swiss-Prot:Q04564" FT /protein_id="AAA98149.1" FT /translation="MANFFYSRITNRSYSTKNLLNIEDKGRLKDELEKTRQTNICEICL FT HPRGHRASVCRQILMGFAYTSKTVLEGLLSTMPHDDAPLGKIFVTDLPPYDEQVPQVLL FT MQAAALVTSYEYSFEDLAYFLVLPLQMALMQKSPSLGKDFPVISGYSITKDSVHSPVAF FT GRNLMPRGVSCEFPQIDVLYDYRGFLEGGAFSEGVTSFPKETKKPKVAVIGAGISGLVS FT ATLLLRNGIDDVTIFEAKNVVGGRAHTHFFKGEPSVCAELGAMRFPRSQACLFYLLEYL FT GINAMTKFPNPGTVDTGLYYRGRSYNWKAHSLPPAIFNRVHKGWRTFLHAGFVDGVAAF FT ASPFTLTECLRLRNYEFASSLWQKWLDAFSSETFSSGIERIFRGAHPPGGEKWTRDVDM FT ELFKELGVGSGGFGPVFGCGFIEILRLIVNGYEDNVMLLLDGIEEIPRRLSQQKVGSYS FT IRDRIIHKEVKEIIRTESGISLAIGEGMHATFDRVIVTSGFTNIQLRHLLTNDDSFFSY FT DVNQAIENSHMTGSSKLFVLTQNKFWKAEELPSCILTTGVAKAVYCLDYEPDKPSGKGL FT VLLSYTWEDDSHKLLTFDKGERFQILKRDLAKSYPRFADLLEPADGDYDNNIIQHDWIL FT DPYAGGAFKLNRRCEDVYSKRLFFQPLRLNGEPDGRVCLAGCSCSFSGGWVEGAIQTAC FT NAAMATIRDAGGLISGDNPLTNEFVNYHY" FT TATA_signal complement(3182..3188) FT /gene="iaaM" FT polyA_signal complement(4817..4822) FT /gene="iaaM" FT repeat_region complement(5206..5277) XX SQ Sequence 5516 BP; 1509 A; 1266 C; 1273 G; 1468 T; 0 other; gaattcttct tcgtgttcgt catagggcga aatcctatca aaggttaggg attcgctctc 60 cacttcttcc gggcaagtcc aacccttgat ccgctcccac tgatcatcat cccgcagtgc 120 atcgcaatcc atcaccgaac tccaaaagcc agtgttgaac ctgatttgac gccccgcggg 180 aatcccaaaa acgtaaatcg tcaccacagc ctaggcgcag acatagaagc tcacttcggc 240 tgacaacgtc cgctaccgat atccgaccgc taaccctctt cgccagacgg ggtcgccttg 300 ccgcttacgg tgcatcgttg agccataaac agcatcgccc cctacacgac caagtggctg 360 gtggcaggat gtattgtcat gtaaacaaaa tgctgtaatt tgtgatgagt acccgcaatc 420 aaataatcct agccgtagca ttcttattgt tcaattaagc tcgtcgtcat aagaagaaaa 480 aattgtgata aaataattga ttccgtagtt cattgagaag caagattgga ccccaagccg 540 tcaaatgtcg taagtttcgg tgtccgtctt taatataatt gtttgttcaa tatttctaat 600 aatacaaacg taagtatgac gcgttatact ttattaatta aaatcacgat ttttacaata 660 tattgagaat tcccgttatt gaaaatgcga tggatgttaa tgatgctaat atttagttag 720 ggttctgcga ggtttaattg gagcggtatt tatataatat agaaaatgaa ttgcaaaaac 780 ataaaaataa ataatatatg tttgctacat accccactaa tgggcaaagg aaacttactg 840 aaattaaata ttactaatat tcttgaccga atttgaccat ttcctctaca gcacgcccga 900 tcgctaacac catatcgtcc gacccaaacg ggccctcgat ctgtattcca accggtagcc 960 tgtcgggtgt caacgacact gggacgctca agcttggcag cccgatgtta ctgctaggat 1020 ctaggtttcg aacgaaaatt ccaaaggtat cagtctcgac gccgttgtgt acaagcgtag 1080 tctgcagccc tactggtttg gcggttaggg gaactgaagg aaataaaaca gcgtcaaggc 1140 cgttggagga gtaataattc tcatatgttg cttttagcct gggcttgtag caccgaagcg 1200 ccctccggta gaccgactct gaaataagtc cttttgatag ttgagattgc acgaggtcat 1260 agacctgtgg atcctgaata ttctgtatca actcatcaaa cgtgtttcca atcccgtgaa 1320 acgagagata ggccatgagc gctcggggaa actcataaat agcaactggc aagctcactt 1380 tgcgggttag ctccgcaaca ttcggaatgt ccgcttccac gaacgtcatt tggttggtag 1440 ccagacgtcg aattgctcgt tccgaggctg ccgcaacatg cggttcgaga ttatcaaaga 1500 agtgggatcg aggcagaccc aaccgaagcc ccttgagtga catattttga gtaggacact 1560 tgttgcgaat gatccgatcc aatagaaccg catcttcaac cgagcggaca atgaggccag 1620 gagtgtctct cgacggactc accggcacaa tgccatcggt tggatagttg ccaaatgttg 1680 gacgaaaacc aacaacccca catagtgccg ccggaagcct aacagaagcg ccggtgtcag 1740 ttcctatgcc gcccaacatc aaattggctg ctaccgagac agcaacgccc ccactcgatc 1800 caccagagat cagagcctgg ttccaagggt ttcgtgcggg accaaacgtc ttgttgtcat 1860 ttgttatacc aaaagataac tcgtgcatat ttccagacgc cccaatcagt gcaccggctg 1920 aatgaagtcg ctctactatc tttgccggaa tagctggctt gtgccgaagc agtccctgtg 1980 tgccagcgga cgtcgggaat attccagtcg ctatgtttgc tttgaaacaa agggggatac 2040 ccataaggcc caccccgccg gatccgttcc tatccacacg atctgcctca tttcttagat 2100 gaagccagtc agttgcagcg aaggcgttca agtgggttgc agattccgaa cgggagacca 2160 aagactcaac taggtcccga catgaatact gtttcctcct tagagctctc aacgtctgcg 2220 aaattgatga tatttcaacc attttactag caagaccggc tctatttttt tgatattata 2280 tgacgagtac ccttaattat aattttgtaa tacaattaca ccgccaatta cggggttaaa 2340 ttcaatgagt tgactttgcc cgacagtggg tcccttcaat tttgaatagg cgtgcaagga 2400 tactgttgca gattgaagac ttttcttccg cacaaccctc cagaccagct ctgcaaattg 2460 tcttcgtggg acctacacta ttaatcaaaa tgatttttcg gttaaaagtt acttctataa 2520 aagatgcttt aagcacagag cagaaacagc atcgaaatat ttcttatttt caatggctaa 2580 ttttttctat tctagaatca caaacagaag ctactccacc aagaacttgc tcaacattga 2640 agataaaggt cgcttgaaag atgagctcga gaagacgcgg caaaccaaca tttgcgagat 2700 ttgcttgcat cctagggggc acagagcaag cgtctgtcga caaatattga tggggttcgc 2760 ttacacaagc aaaaccgtgc tggaaggttt actttcgaca atgcctcatg atgacgcccc 2820 gcttgggaaa atcttcgtaa cggatttgcc gccctatgat gagcaagtcc ctcaagtcct 2880 gctgatgcaa gctgcagcac tagtcacaag ctacgaatat tcctttgaag atttagcata 2940 tttcttggtc ctaccgctgc agatggctct aatgcaaaag tctccaagtc ttgggaagga 3000 ttttccggta atttctggtt attcgatcac aaaagacagt gtgcactctc ctgtcgcgtt 3060 tggaagaaat ttgatgccta ggggcgtcag ttgtgagttt cctcaaatcg atgttctcta 3120 cgactatcgc ggatttctcg agggaggagc ttttagcgaa ggggtcacct ctttccctaa 3180 agaaactaaa aaaccaaaag ttgccgtcat tggagccggt atttcaggat tggtttccgc 3240 aacacttttg ctgcgaaacg gtatcgacga cgttaccata ttcgaagcca aaaacgtggt 3300 gggaggtaga gcccacacac acttcttcaa aggcgaaccg tcggtctgcg cggaacttgg 3360 tgcaatgagg tttcctcgca gccaagcttg cttgttctac ctgctggaat atctaggaat 3420 caatgcaatg actaaatttc caaatccagg aacggttgat accggcttgt actaccgcgg 3480 gagaagctac aactggaaag ctcatagcct ccccccagca attttcaatc gagttcataa 3540 aggctggcgt acattcttgc acgcgggttt tgtagatggt gttgcagcat tcgcgtcccc 3600 gtttacacta actgagtgtc tccggcttcg taactatgag ttcgcttcct cgctttggca 3660 gaagtggctg gatgccttca gcagcgaaac attttcgtcc ggaatagaga gaatctttag 3720 gggtgctcac ccaccaggtg gggagaaatg gaccagagat gtcgacatgg agctattcaa 3780 agagctcgga gtggggtcgg gcggattcgg tccggtattt gggtgcggct tcatcgagat 3840 attgcggttg atagtgaatg gctatgaaga caatgtaatg ttgctgcttg atggaataga 3900 ggaaatccca cgaaggctat ctcagcagaa agttgggtca tattcaatcc gggatcggat 3960 aatccacaaa gaggtgaagg agataattag gaccgagtca ggaatctcgc tagctattgg 4020 cgaaggaatg catgcaacct ttgatcgcgt gatcgtaaca tctggcttta cgaatattca 4080 gttgaggcat ctcttaacca atgatgacag ctttttttct tacgacgtta accaagcgat 4140 cgagaacagc cacatgacag gctcatcaaa gctgtttgtt ctcacgcaga ataagttctg 4200 gaaagctgaa gaactccctt cctgcatact gacaaccggt gttgccaaag cagtttactg 4260 tctggattac gagcctgata aaccgagcgg aaaaggcctt gtacttttga gctacacttg 4320 ggaagacgat tcccataaac tcctgacatt cgataaagga gaaagatttc aaatcttgaa 4380 gcgcgatctc gcaaaatcat atcctagatt tgcggatttg cttgagccgg ctgatgggga 4440 ttacgataac aacatcatcc agcacgactg gattctggac ccatatgcag gcggtgcttt 4500 caagttaaat cgccgatgcg aagacgtcta ttcaaaaagg cttttctttc agccgcttcg 4560 actgaacgga gagcctgatg gacgtgtctg tttggccggg tgcagttgtt ccttttctgg 4620 cggatgggtg gagggcgcta tccagactgc atgtaacgct gcgatggcaa cgattagaga 4680 tgctggcggc ttaattagcg gcgataaccc cctgacaaac gagttcgtta actatcatta 4740 ctagagtagt gtctacctgt agacataaaa aggatgctat aacgcataac gttacgtttt 4800 ttatttcgta taatgtaata aaatattcta gttatgtgta tatgtgaaaa atagcgaaaa 4860 taattgcaaa ttagagtagt aagtattata atggatggta ccaaacgcaa atttaattgc 4920 aaaagtattt cagagcgata atggaatgct tattaaagca ttaatacatc ttctagcaat 4980 acttcgaaat gtatttatat tatggaattt tttttaaaat ttgggttgat taatgctagc 5040 gtcagctatt tagcgagaaa gaatttggtc aaaggtcgcc tgtctacctg ctccaaagtc 5100 aaatgttgaa acagggcaat agcaaacaat tacaacgttg aattctcctg gaagacaacg 5160 gcgcccttat tgtgtaagtc ttgacaggat atatggtgat gtcacgtgca tccgtgcatc 5220 cgtgcatccg tgcatccgtg catccgtgca tccgtgcatc cgtgcatccg tgcatccgtc 5280 gcacccctcc ggcgttgctt gcaattaccc acatttgagt gtcgccgcat ttcggttcca 5340 aggcgaatat ctgtcagccg actgaggccg cgccagacaa ccgtatttcc cggatgcgga 5400 tctgatgttc ttgcaaggta accgcccaaa cgagcgagtt tgatgagata gaagtttaac 5460 gatctcagcg tcgcctgcct gttgcctgcg ttgctgacca tttgatcgag gatatc 5516 // |
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