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EBIDatabasesDatabase BrowsingDbfetch

EBI Dbfetch

ID   M87289; SV 1; linear; genomic DNA; STD; PRO; 1612 BP.
XX
AC   M87289;
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DT   01-MAR-1992 (Rel. 31, Created)
DT   17-APR-2005 (Rel. 83, Last updated, Version 5)
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DE   Lactococcus lactis methylase (SCRFI) gene, complete cds.
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KW   ScrFI methylase; SCRFI restriction modification system.
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OS   Lactococcus lactis
OC   Bacteria; Firmicutes; Lactobacillales; Streptococcaceae; Lactococcus.
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RN   [1]
RP   1-1612
RX   PUBMED; 8481004.
RA   Davis R., van der Lelie D., Mercenier A., Daly C., Fitzgerald G.F.;
RT   "ScrFI restriction-modification system of Lactococcus lactis subsp.
RT   cremoris UC503: cloning and characterization of two ScrFI methylase genes";
RL   Appl. Environ. Microbiol. 59(3):777-785(1993).
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FH   Key             Location/Qualifiers
FH
FT   source          1..1612
FT                   /organism="Lactococcus lactis"
FT                   /sub_species="cremoris"
FT                   /strain="UC503"
FT                   /mol_type="genomic DNA"
FT                   /tissue_lib="Cremoris/322/4"
FT                   /db_xref="taxon:1358"
FT   -35_signal      118..123
FT                   /gene="SCRFI"
FT   -10_signal      141..146
FT                   /gene="SCRFI"
FT   RBS             193..199
FT                   /gene="SCRFI"
FT   CDS             205..1374
FT                   /codon_start=1
FT                   /transl_table=11
FT                   /gene="SCRFI"
FT                   /product="SCRFI methylase"
FT                   /db_xref="GOA:P34877"
FT                   /db_xref="InterPro:IPR001387"
FT                   /db_xref="InterPro:IPR001525"
FT                   /db_xref="InterPro:IPR010982"
FT                   /db_xref="InterPro:IPR018117"
FT                   /db_xref="UniProtKB/Swiss-Prot:P34877"
FT                   /protein_id="AAA25220.1"
FT                   /translation="MTTISRNTGTEISIMIKEKRLRLNMTQKELADAVGMSKNGDRTIR
FT                   RWENGETCPSQLEISAILRFPEIAPFENRKTAKYKMIDLFAGIGGTRLGFHQTEKVKSV
FT                   FSSEIDKFAIKTYKANFGDEPHGDITKIDEKDIPDHDILVGGFPCQAFSQAGKKLGFDD
FT                   TRGTLFFEIARIIKEKRPKAFLLENVKNLKTHDKGRTFKTILNTLEELDYEVHTALFKA
FT                   RDFGLPQNRERIYIVGFDRKSISNYSDFQMPTPLQEKTRVGNILESVVDDKYTISDKLW
FT                   DGHQRRKTENKKNGKGFGYTLFNQDSEYTNTLSARYYKDGSEILIEQKNKNPRKITPRE
FT                   AARLQGFPENFIIPVSDTQAYKEFGNSVAVPTIHAIAEKMLEVLEKSKK"
FT   stem_loop       1377..1417
FT                   /gene="SCRFI"
FT                   /note="17 bp stem structure with an 8 bp loop region
FT                   located 1 bp downstream of the stop codon."
XX
SQ   Sequence 1612 BP; 627 A; 238 C; 261 G; 486 T; 0 other;
     agctactgga atagtttaat taacggattg ccaccaataa gaaatatgag catctaataa        60
     aaaattaaat tttggaacga tatttattcg ttcttttttc tttaataaaa ataatccttg       120
     caaatcattg aatttattgc tataattagt gtcaatcaga ggacaaaaag tccttataaa       180
     aacactttat gagaaaggat ttatatgact actatatcaa gaaatacagg aacagaaatt       240
     tctataatga ttaaagaaaa gcgactacgc ctaaatatga ctcaaaaaga gcttgcagat       300
     gcagtgggca tgtcaaaaaa cggagataga accataagaa gatgggaaaa tggtgaaact       360
     tgtccatcac agcttgaaat atctgctatt ttaagatttc cagaaattgc accatttgaa       420
     aatagaaaaa cagctaaata taaaatgatt gacctatttg caggtattgg agggacacgc       480
     ttaggttttc atcaaacaga aaaagtaaag tctgtatttt catctgaaat agataaattt       540
     gctattaaaa cttataaagc gaattttgga gacgaacctc atggggatat aacaaaaatt       600
     gatgagaaag atatcccaga ccatgatatt cttgtcggtg gatttccttg tcaagcattt       660
     agtcaagcag gaaaaaaatt aggatttgat gatacacgtg gaactttatt ttttgaaata       720
     gcaagaatta taaaagaaaa acgccctaaa gcatttctac tcgaaaatgt aaaaaattta       780
     aaaacccatg ataaaggaag aacttttaaa acaatattaa atactttgga agaacttgat       840
     tatgaagttc acacagcgct attcaaagca agagattttg gtcttccaca aaatagagaa       900
     aggatttata ttgtaggttt tgacagaaaa tcaattagca actattctga ttttcaaatg       960
     cctacacctt tacaagaaaa aactcgtgtt ggaaatattt tagaatccgt tgttgatgat      1020
     aaatatacaa tatcagataa actttgggat gggcatcaaa gaagaaaaac tgaaaataaa      1080
     aaaaatggca aaggttttgg atataccctc tttaatcaag atagtgagta tacaaacact      1140
     ttatcagctc gttattataa agatggtagt gaaatactaa ttgaacagaa aaataaaaat      1200
     cctcgaaaaa ttactcctag ggaagctgct cgattacaag gttttccaga aaattttatt      1260
     atacctgtta gcgataccca agcttataaa gaatttggaa actctgttgc agttcccacc      1320
     attcatgcta ttgcagagaa gatgttggaa gttttagaga agtcaaaaaa ataactaaaa      1380
     aatctcacgg attaaatctt atccatgaga ttttttatat tctccaaatc ttcttttctc      1440
     tgtactatta cgatattaga cgtattttga acagaggaat agttgatctc ccttatttcc      1500
     tcatctgtta gattatatac tttgctatca aaaagaatca tctcaccatt caattttatc      1560
     tttacggcac caatacaacg cttaggggaa acaggtaaca tatagtcagc at              1612
//
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