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EBI DbfetchID M86545; SV 1; linear; genomic DNA; STD; UNC; 2648 BP. XX AC M86545; XX DT 01-OCT-1996 (Rel. 49, Created) DT 15-JAN-2007 (Rel. 90, Last updated, Version 4) XX DE Plasmid P4 (from Shigella sonnei strain 47) C5-cytosine methylase (MSsoII) DE gene, complete cds and putative promoter region; restriction endonuclease DE (RSsoII) gene, complete cds and putative promoter region. XX KW C5-cytosine methylase; MSsoII gene; restriction endonuclease; RSsoII gene. XX OS Plasmid P4 OC other sequences; plasmids. OG Plasmid P4 XX RN [1] RP 1-2648 RX DOI; 10.1016/0378-1119(93)90756-S RX PUBMED; 7916706. RA Karyagina A.S., Lunin V.G., Degtyarenko K.N., Uvarov V.Y., Nikolskaya I.I.; RT "Analysis of the nucleotide and derived amino acid sequences of the SsoII RT restriction endonuclease and methyltransferase"; RL Gene 124(1):13-19(1993). XX DR RFAM; RF00106; RNAI. XX FH Key Location/Qualifiers FH FT source 1..2648 FT /organism="Plasmid P4" FT /plasmid="P4" FT /host="47" FT /mol_type="genomic DNA" FT /db_xref="taxon:28398" FT terminator 6..29 FT /gene="MSsoII" FT /note="putative" FT gene 6..1387 FT /gene="MSsoII" FT CDS complement(163..1302) FT /codon_start=1 FT /transl_table=11 FT /gene="MSsoII" FT /standard_name="SsoII methylase" FT /product="C5-cytosine methylase" FT /db_xref="GOA:P34879" FT /db_xref="HSSP:1FJX" FT /db_xref="InterPro:IPR001387" FT /db_xref="UniProtKB/Swiss-Prot:P34879" FT /protein_id="AAA98279.1" FT /translation="MTDNIAATIKEKRERLHMTQKEFADALGLSKYGDRTIRRWERGET FT KPTGAELKAVIDFPDTPPYPNNENGRYRMIDLFAGIGGTRLGFHQTNAVNVVFSSEWDK FT FAQKTYHANYGDFPDGDITKIDEKDIPDHEILVGGFPCVAFSQAGLKKGFNDTRGTLFF FT DIARIIKEKKPHAFLLENVKNLLGHDKGRTFSIIKNTLEELNYTVYYNIFAAKDFGVPQ FT NRERIYIVGFNKEKVRNHEHFTFPTPLKTKTRVGDILEKSVDNKYTLSDALWNGHQRRK FT LVNAAAGKGFGYGLFNENSPYTNTISARYYKDGSEILIEQKGSNPRKITPREASRLQGF FT PSDFIIPVSDTQAYKQFGNSVAVPVINAIAEKIISTLDS" FT RBS complement(1309..1318) FT /gene="MSsoII" FT /standard_name="Shine-Dalgarno" FT /note="putative" FT -35_signal 1334..1337 FT /gene="RSsoII" FT /note="putative" FT gene 1334..2418 FT /gene="RSsoII" FT -10_signal 1355..1360 FT /gene="RSsoII" FT /note="putative" FT -10_signal 1359..1364 FT /gene="MSsoII" FT /note="putative" FT -35_signal complement(1382..1387) FT /gene="MSsoII" FT /note="putative" FT RBS 1394..1403 FT /gene="RSsoII" FT /standard_name="Shine-Dalgarno" FT /note="putative" FT CDS 1412..2329 FT /codon_start=1 FT /transl_table=11 FT /gene="RSsoII" FT /standard_name="SsoII endonuclease" FT /product="restriction endonuclease" FT /db_xref="GOA:P34880" FT /db_xref="InterPro:IPR015109" FT /db_xref="UniProtKB/Swiss-Prot:P34880" FT /protein_id="AAA98280.1" FT /translation="MQRLSPGEFKTLISKERKSHFITPFALVYKTFCDLGYDQKNSDYF FT LNNPSEYIIAMRKNCWKEFEPFEKEFTTRMLSYLIDEERIKDMSPYDAIRDFTMEYPTH FT IYDLALSNTQSRRSRAGKEFESILELLMMGAGIPVDVQGAIGKSFFQKNQIGKLVDLVM FT PGVVQYTSNKRNTMLISAKTTLRERWQEVPEEVNRTGIREMYLATLDDSFSEETINILY FT EANVVVVTTIENKNFKYKNNNRVLTFEDMLQSAMELSRKWNNVSYTDSEKEEIQRSILK FT QIEKYSDFPYVVNYYRNRLSALFD" FT terminator 2335..2358 FT /gene="RSsoII" FT /note="putative" FT terminator 2387..2418 FT /gene="RSsoII" FT /note="putative" XX SQ Sequence 2648 BP; 789 A; 462 C; 517 G; 880 T; 0 other; acgcgttttt ccggtttacc ccgggaaaaa acatctcttt ttgcggtgtt cgcggcagaa 60 tcgcgttcag cgcgttttag cggtgcgcgt aatgcgacgt tatggtaaat ccatatgggg 120 ggtaattgga ttctgggccg cattgtgtcc tcatgactta gtttatgaat ctaaggtcga 180 tatgattttt tctgctattg cattgataac agggactgcg actgaattcc caaattgctt 240 ataagcctgt gtatccgata cagggataat aaaatcacta ggaaaccctt gaagtcttga 300 cgcttcccgc ggagttattt ttcttgggtt acttcctttt tgctctatta atatttcgct 360 accatcttta tagtacctcg cacttatggt attagtatat gggctatttt cattaaataa 420 accataccca aagcctttac cggctgctgc atttacaagt ttcctgcgtt ggtgtccatt 480 ccagagcgca tctgatagcg tatatttatt gtctacgctc ttttcaagaa tatctccaac 540 tcttgttttt gtttttaatg gggttggaaa agtaaaatgt tcatgatttc ttactttttc 600 tttattaaag cctacaatgt atatgcgctc tctattttgt ggaacaccaa agtcttttgc 660 agcgaagata ttgtaataca cagtgtaatt caattcttca agtgtgtttt ttattattga 720 aaaagttcta cctttatcat gtcctagcaa gttttttaca ttttcaagta aaaaagcatg 780 aggttttttt tcctttatta tccttgctat atcaaaaaac aaagttcctc ttgtatcatt 840 aaatcctttt ttgaggcctg cttgagagaa tgctacacaa ggaaacccac cgacaagaat 900 ttcatgatca ggaatatctt tttcatcgat ttttgttatg tccccatcag ggaaatcacc 960 ataatttgca tgatatgtct tttgagcaaa cttatcccat tcgctggaaa aaacaacatt 1020 tacagcgtta gtttgatgaa atccaagtct ggttccaccg attccagcaa ataaatcgat 1080 catgcgatat ctgccattct cgttgtttgg gtaaggtggt gtgtctggaa agtctataac 1140 tgcttttagt tccgccccag taggttttgt ttccccgcgc tcccatcttc ttatggttct 1200 gtctccatat tttgaaagac caagggcgtc tgcaaactct ttttgagtca tatgaagtct 1260 ttctcttttt tctttgattg ttgctgcgat attatcagtc atgcatgtct accagaatta 1320 agtgttggtt ttaggacaat ttgtcctgtt ttgattccaa ttatgaacgt gcatgaaaaa 1380 actttcaagt agatataaaa ggttattttt tatgcaaaga ttaagtcctg gtgaatttaa 1440 aactctaatt tctaaagaga gaaaatcgca ttttattacg ccatttgctt tggtttataa 1500 aacattttgt gatttagggt atgatcaaaa aaatagtgat tattttttga ataatccaag 1560 tgaatatata attgcaatga gaaaaaactg ttggaaagaa tttgaaccat ttgaaaaaga 1620 attcacaaca agaatgttat catatcttat tgatgaagaa agaataaaag atatgagtcc 1680 ttatgatgcc attcgtgatt ttacgatgga ataccctaca cacatatatg atttggcact 1740 gtctaacact caatccagac gtagcagggc aggaaaagag tttgagtcta ttcttgagct 1800 attaatgatg ggggcaggaa ttcctgttga tgtgcagggg gctattggga agtcattttt 1860 tcaaaaaaac caaataggta aacttgtaga tcttgttatg ccaggtgtag tccagtacac 1920 atctaacaaa agaaatacaa tgttgatttc cgctaaaaca acgctgaggg agcgatggca 1980 agaagtacct gaagaggtta atagaacagg aattagagaa atgtatctag ctacactaga 2040 tgattctttt agcgaggaga caataaatat attgtacgag gctaatgtgg tcgttgtaac 2100 gacgatagaa aataaaaatt tcaaatataa aaataataat agggtgctaa cctttgaaga 2160 tatgcttcag tcagcaatgg aattatcaag aaagtggaat aatgtaagtt acacagactc 2220 tgaaaaggag gaaattcaac ggtcaatatt aaaacaaata gaaaaatata gtgatttccc 2280 ttatgttgtt aattattata gaaataggct tagtgctctt tttgactaaa aaactaaaag 2340 gatctcttga gatccttttt ttctgcgcgt aatcttggat ctgtaaacga aaaaaccacc 2400 ctggcaggtg gttttttcga aggttagcta atcctggcag attatctaac cgaggtaatc 2460 tggcttcagc agagcacaga taccaaatac tgtccttcca gtgtagccgt agttaggcca 2520 tcacttcaag aactctgtaa gcatctggat aaatctcgct ctgctaatcc ggttaccagt 2580 ggctgctgcc agtggcgtta aggcgtgtct taccgggttg gactcaagac gatagttacc 2640 ggataagg 2648 // |
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