 |
|
EBI DbfetchID M84911; SV 1; linear; genomic DNA; STD; PRO; 5417 BP. XX AC M84911; XX DT 10-JUL-1992 (Rel. 32, Created) DT 14-NOV-2006 (Rel. 89, Last updated, Version 9) XX DE Pseudomonas aeruginosa methylmalonate semialdehyde dehydrogenase (mmsA) DE gene complete cds, 3-hydroxyisobutyrate dehydrogenase (mmsB) gene complete DE cds, ORF1 5'end. XX KW 3-hydroxyisobutyrate dehydrogenase; KW methylmalonate semialdehyde dehydrogenase; mmsA gene; mmsAB operon; KW mmsB gene; mmsR gene; transcription regulator; valine metabolism. XX OS Pseudomonas aeruginosa OC Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales; OC Pseudomonadaceae; Pseudomonas. XX RN [1] RP 1-5417 RX PUBMED; 1339433. RA Steele M.I., Lorenz D., Hatter K., Park A., Sokatch J.R.; RT "Characterization of the mmsAB operon of Pseudomonas aeruginosa PAO RT encoding methylmalonate-semialdehyde dehydrogenase and 3-hydroxyisobutyrate RT dehydrogenase"; RL J. Biol. Chem. 267(19):13585-13592(1992). XX FH Key Location/Qualifiers FH FT source 1..5417 FT /organism="Pseudomonas aeruginosa" FT /mol_type="genomic DNA" FT /db_xref="taxon:287" FT gene complement(463..1386) FT /gene="mmsR" FT CDS complement(463..1386) FT /codon_start=1 FT /transl_table=11 FT /gene="mmsR" FT /function="regulatory gene for mmsAB operon" FT /note="member of the AraC/XylS family of transcriptional FT regulators" FT /db_xref="GOA:P28809" FT /db_xref="InterPro:IPR018060" FT /db_xref="UniProtKB/Swiss-Prot:P28809" FT /experiment="experimental evidence, no additional details FT recorded" FT /protein_id="AAA25890.1" FT /translation="MDYIAEPATDMSHTSDWPLPADSLRLPIPAALLEKLAVHPLSRDL FT YPTSLGHYRRARDHRMSRERHDEHLLIYCSEGQGLLRVREGEAWREYRVGSGDLLWLPP FT GMAHDYAADDRQPWTIFWTHLRGDTATWLQHSAGYRDTPLRHWGLQHALLGGFEQLLEV FT RHSGYRFQHFLLAASRLRSLLCQLPLLRPLREGSLDLDGLHAYMREHLHARLELERLAA FT FCNLSKFHFVSRYKAITGRTPIQHFLHLKIEYACQLLDSSDQSVARVGQAVGYDDSYYF FT SRLFSKVMGLSPSAYRQRVRQGEGGA" FT mRNA 1441..3923 FT /gene="mmsAB" FT /note="joint mRNA encoding mmsA and mmsB gene" FT /experiment="experimental evidence, no additional details FT recorded" FT gene 1441..3923 FT /gene="mmsAB" FT RBS 1509..1520 FT /gene="mmsA" FT /note="putative" FT gene 1509..3011 FT /gene="mmsA" FT CDS 1518..3011 FT /codon_start=1 FT /transl_table=11 FT /gene="mmsA" FT /product="methylmalonate semialdehyde dehydrogenase" FT /function="catalyzes step in distal pathway of FT valinemetabolism, located on a operon with gene FT for3-hydroxyisobutyrate dehydrogenase" FT /EC_number="1.2.1.27" FT /db_xref="GOA:P28810" FT /db_xref="HSSP:1BXS" FT /db_xref="InterPro:IPR010061" FT /db_xref="UniProtKB/Swiss-Prot:P28810" FT /experiment="experimental evidence, no additional details FT recorded" FT /protein_id="AAA25891.1" FT /translation="MSVPVRHLIAGAFVEGLGAQRIPVSNPLDNSTLAEIACASAEQVE FT QAVASARETFASWKETPVSERARVMLRYQALLKEHHDELAKIVSSELGKTFEDAKGDVW FT RGIEVVEHACNVPSLLMGETVENVARNIDTYSITQPLGVCVGITPFNFPAMIPLWMFPL FT AIACGNAFILKPSEQVPLTSVRLAELFLEAGAPKGVLQVVHGGKEQVDQLLKHPQVKAV FT SFVGSVAVGQYVYHTGTAHNKRVQSFAGAKNHMVIMPDADKAQVISNLVGASVGAAGQR FT CMAISVAVLVGAAREWIPEIRDALAKVRPGPWDDSGASYGPVINPQAKARIERLIGQGV FT EEGAQLLLDGRGYKVEGYPDGNWVGPTLFAGVRPDMAIYREEVFGPVLCLAEVDSLEQA FT IRLINESPYGNGTSIFTSSGAAARTFQHHIEVGQVGINIPIPVPLPFFSFTGWKGSFYG FT DLHAYGKQGVRFYTETKTVTARWFDSDSVAGTNFSIQMR" FT mat_peptide 1521..3008 FT /gene="mmsA" FT /product="methylmalonate semialdehyde dehydrogenase" FT /experiment="experimental evidence, no additional details FT recorded" FT gene 3015..3923 FT /gene="mmsB" FT RBS 3015..3029 FT /gene="mmsB" FT /note="putative" FT CDS 3027..3923 FT /codon_start=1 FT /transl_table=11 FT /gene="mmsB" FT /product="3-hydroxyisobutyrate dehydrogenase" FT /function="catalyzes step in distal valine metabolic FT pathway, located on an operon with methylmalonate FT semialdehyde dehydrogenase" FT /EC_number="1.1.1.31" FT /db_xref="GOA:P28811" FT /db_xref="InterPro:IPR016040" FT /db_xref="UniProtKB/Swiss-Prot:P28811" FT /experiment="experimental evidence, no additional details FT recorded" FT /protein_id="AAA25892.1" FT /translation="MTDIAFLGLGNMGGPMAANLLKAGHRVNVFDLQPKAVLGLVEQGA FT QGADSALQCCEGAEVVISMLPAGQHVESLYLGDDGLLARVAGKPLLIDCSTIAPETARK FT VAEAAAAKGLTLLDAPVSGGVGGARAGTLSFIVGGPAEGFARARPVLENMGRNIFHAGD FT HGAGQVAKICNNMLLGILMAGTAEALALGVKNGLDPAVLSEVMKQSSGGNWALNLYNPW FT PGVMPQAPASNGYAGGFQVRLMNKDLGLALANAQAVQASTPLGALARNLFSLHAQADAE FT HEGLDFSSIQKLYRGKD" FT CDS 4025..5417 FT /partial FT /codon_start=1 FT /transl_table=11 FT /note="ORF located downstream of mmsAB operon, has sequence FT similarity to an acetyl-CoA synthetase; ORF1; putative" FT /db_xref="GOA:P28812" FT /db_xref="HSSP:1PG4" FT /db_xref="InterPro:IPR000873" FT /db_xref="UniProtKB/Swiss-Prot:P28812" FT /protein_id="AAA25893.1" FT /translation="MTYLQQHARSIAEPAAFWAEQARSLAWYQAPANILESLPDGTHRW FT FADGRLNSAYLALDRQIEEGRGEQTALIYDSPVTGTQDRYSYLRLRDEVARLAGALRAL FT GVGKGDRVIIYMPMVPQAAMAMLACARLGAVHSVVFGGFAPYELALRIDDATPKLVLTA FT SCGLEFDRVIEYKPLVDKALELAIHQPAHVMVWQRPQAPARLHPGRDLDWQDCLAAAEP FT ADPLPVASGDPLYIMYTSGTTGKPKGIVRDNGGHAVAVRYAVRTIYGMQAGDVWWGISD FT VGWVVGHSLIVYGPLMCGCTTVFYEGKPVRTPDAGAYWRVIEEHRVNSLFCAPTAIRAI FT RKEDPHGERVKRYDLGSLRHLFLAGEKLDSSTQHWLEEHTGRPVHDHWWQTETGWPVTA FT PCIGIAGHDLRAGSTNRAVPGYHVQVLDDEGRPLGANRQGAIVIALPLPPGCAQTLWGD FT HPRYLQ" XX SQ Sequence 5417 BP; 881 A; 1832 C; 1880 G; 824 T; 0 other; ctgcagttca gcgtggcggc gggcgcttcg gcgctggtcg gcgtgctcca cgacggcacg 60 gcgatcccca tgactctggt gatcgccgtc tgcggcgcgg tcgcgaccct gctcgcctgg 120 cagaccgggc gcctggagcg ccaggccgcc tgagcgcgga tcaggccgtt cgctgtttca 180 gttcgggcag cacatggggc gcgcgcaggc ggccctcgag gcggtcgacg aaacgccggg 240 cctcgctttc gctacggaag ttgacggcct gctggtcgag gcagacttgc cagcattggc 300 ctttcggggt ttgcgacggg cggacgagga tgttcatggc gattcatcca tcggggctgg 360 aaagggagct tcagtctagt ctgtcggccc ggaaaacact gaccgggatc agtttgccga 420 caactggtcg ccgcttcgtt tacagccccg ccgcagagcg cttcaggctc ctccctcgcc 480 ctgccttacc cgctgccggt aggcgctcgg cgacaaaccc atgaccttgc tgaacaggcg 540 cgagaagtag tagctgtcgt cgtagccgac cgcctggccg acccgggcca cgctctggtc 600 gctgctgtcc agcaactggc aggcgtactc gatcttcagg tgcaggaagt gctggatagg 660 cgtacggccg gtgatcgcct tgtagcgact gacgaaatgg aacttcgaca ggttgcagaa 720 ggccgccagg cgttccagtt cgaggcgcgc atgcaggtgt tcgcgcatgt aggcgtgcag 780 cccgtcgagg tccaggctgc cctcgcgcag cgggcgcagc agcggtagct ggcagagcag 840 gctgcgcagg cggctggcgg cgagcaggaa gtgctggaag cggtagccgc tgtggcgcac 900 ctccagcagt tgctcgaagc cgccgagcag ggcgtgttgc aggccccaat ggcgcagcgg 960 ggtgtcgcgg tagccggcgc tgtgttgcag ccaggtggcc gtgtcgccgc gcaggtgggt 1020 ccaaaagatg gtccagggtt ggcggtcgtc ggcggcgtag tcgtgggcca tgcccggcgg 1080 cagccagagc aggtcgccgc tgccgacccg gtattcgcgc caggcctcgc cctcgcgcac 1140 gcgcaacagg ccctggcctt cgctgcaata gatcagcagg tgctcgtcat ggcgctcgcg 1200 gctcatgcgg tggtcgcggg cgcgccggta gtggcccagg gaggtggggt agaggtcgcg 1260 gctgagcggg tggaccgcca gtttctccag cagcgccgcc gggatcggca agcgcaggct 1320 gtcggccggc aggggccagt cggaggtgtg cgacatgtcg gtagccggct ccgcaatata 1380 gtccatgaac aacacaattt agtcgatctc gcgaccttcc gcccagtgat agcttcttgg 1440 ccaaagcgag gtccgaatcg acctcgtacc agtccgccac aacaataaaa gccggcctgg 1500 ccggtgcagg agggttcatg tcagtcccag tccgccacct catcgcggga gccttcgtcg 1560 aaggtctcgg cgcccagcgc atcccggtca gcaatcccct cgacaacagc accctggcgg 1620 agatcgcctg cgccagcgcg gagcaggtcg agcaggcggt ggccagtgcc cgcgaaacct 1680 tcgccagctg gaaggaaacc ccggtttccg agcgcgcgcg ggtgatgctg cgttaccagg 1740 cgttgctcaa ggaacaccac gacgaactgg cgaagatcgt ctccagcgag ctgggcaaga 1800 ctttcgagga cgccaagggc gacgtctggc gcggtatcga ggtggtcgaa cacgcctgca 1860 acgtgccctc gctgctgatg ggcgagacgg tggagaacgt cgcccgcaac atcgacacct 1920 acagcatcac ccagccgctg ggcgtgtgcg ttggcatcac cccgttcaac ttcccggcga 1980 tgatcccgct gtggatgttc ccgctggcca tcgcctgcgg caacgccttc atcctcaagc 2040 cttccgagca ggtgccgctg accagcgtgc ggctcgccga gctgttcctc gaggccggtg 2100 cgccgaaggg cgtgctgcag gtggtgcatg gcggcaagga acaggtcgac cagttgctca 2160 agcacccgca ggtgaaggcg gtgtccttcg tcggctcggt cgccgtcggc cagtacgtct 2220 accacaccgg caccgcgcac aataagcgcg tgcagagctt cgccggggcg aagaaccaca 2280 tggtgatcat gcccgacgcg gacaaggcac aggtcatcag caacctggtc ggtgcctcgg 2340 tgggcgcggc cgggcagcgc tgcatggcga tctcggtggc ggtgctggtg ggggccgccc 2400 gcgagtggat tccggaaatc cgcgacgcgc tggccaaggt ccgccccggc ccatgggacg 2460 acagcggcgc cagctacggc ccggtgatca acccgcaggc caaggcgcgc atcgagcgcc 2520 tgatcggcca gggcgtggag gagggcgcgc aactgctgct ggatggccgc ggctacaagg 2580 tcgagggata cccggacggc aactgggtcg gcccgacgct gttcgccggg gtgcgcccgg 2640 acatggcgat ctaccgcgaa gaggtgttcg gcccggtgct ctgcctggcc gaggtcgaca 2700 gcctggagca ggcgatccgc ctgatcaacg aaagccccta cggcaacggc acctcgatct 2760 tcaccagctc cggcgccgcg gcgcggacct tccagcacca catcgaggtc ggccaggtgg 2820 gcatcaacat cccgattccg gtaccgctgc cgttcttctc cttcaccggt tggaagggct 2880 cgttctacgg cgacctgcac gcctacggca agcagggcgt gcgcttctac accgagacca 2940 agaccgtgac cgcgcgctgg ttcgacagcg acagcgtggc cgggaccaac ttctcgatcc 3000 agatgcgctg aaagggagac ggaccgatga ccgacatcgc attcctcggc ctgggcaaca 3060 tgggtgggcc gatggccgcc aacctgctca aggccggcca ccgggtgaat gtcttcgact 3120 tgcagcccaa ggccgtgctg ggcctggtcg agcagggcgc gcagggcgcc gatagcgcct 3180 tgcagtgctg cgaaggcgcc gaagtggtga tcagcatgct gccggccggg cagcacgtgg 3240 aaagcctgta tctcggcgac gacggcctgc tcgcgcgggt cgccggcaag cccctgctga 3300 tcgactgctc gaccatcgcc ccggagaccg cgcgcaaggt cgccgaggcc gccgcggcga 3360 agggcctgac cctgctcgac gcgccggttt ccggcggcgt cggcggcgcc cgcgccggca 3420 ccctgagctt catcgtcggc ggccccgccg aaggcttcgc gcgggcccgg ccggtcctcg 3480 agaacatggg ccggaacatc ttccacgccg gcgatcacgg cgccggccag gtggcgaaga 3540 tctgcaacaa catgctcctc ggcatcctca tggccggcac cgccgaggcc ctggcgctgg 3600 gggtgaagaa cggcctcgac ccggcggtgc tgtccgaggt gatgaagcag agttccggcg 3660 gcaactgggc gctgaacctc tacaacccct ggcccggggt gatgccgcag gcgccggcga 3720 gcaacggcta tgccggcggt ttccaggtgc gcctgatgaa caaggacctc ggcctggcgc 3780 tggccaacgc ccaggcggtg caggcctcga cgccgctcgg cgcgctggcg cgcaacctgt 3840 tcagcctgca cgcccaggcc gatgccgagc acgaggggct ggacttctcc agcatccaga 3900 agctctaccg cggcaaggac tgacccctac gaccaaaggt tgatggcggg tgcccggcac 3960 ccgcccatac tccaaggctc actcactgct tcatccctaa taagaacaat cagagggctg 4020 gaacatgacg tacctgcagc agcacgcccg ctcgatcgcc gagccggccg cgttctgggc 4080 cgaacaggcc cgttccctgg cctggtacca ggcgcccgcg aacatcctcg aaagcctgcc 4140 cgacggcacc catcgctggt tcgccgatgg ccgtctgaac agcgcctacc tggccctcga 4200 ccggcagatc gaggaggggc gcggcgaaca gaccgcgctg atctacgact cgccggtgac 4260 cggcacccag gaccgctaca gctacctgcg gctgcgcgac gaagtggcgc gcctggccgg 4320 tgccctgcgg gccctcgggg tcggcaaggg cgaccgggtg atcatctaca tgccgatggt 4380 gccgcaggcg gccatggcga tgctcgcctg cgcgcgcctg ggcgcggtgc attcggtggt 4440 gttcggtggc ttcgctccct acgagctggc cctgcgcatc gacgacgcca cgccgaagct 4500 ggtgctcacc gcttcctgcg ggctggagtt cgatcgggtg atcgaataca agccgctggt 4560 ggacaaggcc ctcgaactgg cgatccacca gcctgcccac gtcatggtct ggcaacgccc 4620 gcaggcgccc gcgcgcctgc acccgggccg cgacctggac tggcaggact gcctcgccgc 4680 cgccgagccg gccgatccgc tgccggtggc cagcggcgat ccgctgtaca tcatgtacac 4740 ctcgggcacc accggtaagc ccaagggtat cgtccgcgac aacggtggcc acgcggtcgc 4800 ggtgcgctac gcggtccgca ctatctacgg catgcaggcc ggcgacgtct ggtgggggat 4860 ctccgacgtc ggctgggtgg tcggccactc gctgatcgtc tacggcccgc tgatgtgcgg 4920 ctgtaccacg gtgttctacg aaggcaagcc ggtgcgcacg ccggacgccg gcgcctactg 4980 gcgggtgatc gaagagcaca gggtgaacag cctgttctgc gcgccgacgg cgatccgcgc 5040 gatccgcaag gaagacccgc atggcgaacg ggtgaagcgc tacgacctgg gttcgctgcg 5100 ccacctgttc ctggccggcg agaagctcga cagcagcacc cagcactggc tggaggagca 5160 caccgggcgg ccggtccacg accattggtg gcagaccgag accggctggc cggtgaccgc 5220 gccctgcatc gggatcgccg gccacgacct gcgggccggc tcgaccaacc gcgcggtgcc 5280 cggctaccac gtgcaggtgc tggacgacga aggccggccg ctgggagcga accgccaggg 5340 cgcgatcgtc atcgccctgc cgttgccgcc gggctgcgcg cagaccctct ggggcgacca 5400 cccgcgctac ctgcagg 5417 // |
|