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EBIDatabasesDatabase BrowsingDbfetch

EBI Dbfetch

ID   M84604; SV 1; linear; mRNA; STD; INV; 1691 BP.
XX
AC   M84604;
XX
DT   03-JUN-1992 (Rel. 32, Created)
DT   17-APR-2005 (Rel. 83, Last updated, Version 8)
XX
DE   Giardia lamblia NADP-dependent glutamate dehydrogenase (NADP-GDH) mRNA,
DE   complete cds.
XX
KW   cytoplasmic protein; NADP-dependent glutamate dehydrogenase.
XX
OS   Giardia intestinalis
OC   Eukaryota; Diplomonadida; Hexamitidae; Giardiinae; Giardia.
XX
RN   [1]
RP   1-1691
RX   PUBMED; 1559991.
RA   Yee J., Dennis P.P.;
RT   "Isolation and characterization of a NADP-dependent glutamate dehydrogenase
RT   gene from the primitive eucaryote Giardia lamblia";
RL   J. Biol. Chem. 267(11):7539-7544(1992).
XX
FH   Key             Location/Qualifiers
FH
FT   source          1..1691
FT                   /organism="Giardia intestinalis"
FT                   /strain="Portland 1"
FT                   /mol_type="mRNA"
FT                   /dev_stage="trophozoite"
FT                   /tissue_lib="lambda gt10"
FT                   /db_xref="taxon:5741"
FT   TATA_signal     198..203
FT                   /gene="NADP-GDH"
FT   CDS             224..1573
FT                   /codon_start=1
FT                   /gene="NADP-GDH"
FT                   /product="NADP-dependent glutamate dehydrogenase"
FT                   /function="catalyzes the reductive amination of
FT                   a-ketoglutarate to L-glutamate"
FT                   /EC_number="1.4.1.4"
FT                   /db_xref="GOA:P28724"
FT                   /db_xref="HSSP:1AUP"
FT                   /db_xref="InterPro:IPR016040"
FT                   /db_xref="UniProtKB/Swiss-Prot:P28724"
FT                   /protein_id="AAA29155.1"
FT                   /translation="MPAQTIEELIAVIKQRDGHMTEFRQAVEEVVDSLKVIFEREPKYI
FT                   PIFERMLEPERVIIFRVPWMDDAGRINVNRGFRVQYNSALGPYKGGLRFHPSVNLSILK
FT                   FLGFEQILKNSLTTLPMGGGKGGSDFDPKGKSDNEVMRFCQSFMTELQRHVGADTDVPA
FT                   GDIGVGAREIGYLYGQYKRLRNEFTGVLTGKNVKWGGSFIRPEATGYGAVYFLEEMCKD
FT                   NNTVIRGKNVLLSGSGNVAQFACEKLIQLGAKVLTFSDSNGTIVDKDGFNEEKLAHLMY
FT                   LKNEKRGRVSEFKDKYPSVAYYEGKKPWECFEGQMDCIMPCATQNEVSGDDATRLVGLG
FT                   LKFVAEGANMPSTAEAVHVYHAKGVMYGPAKASNAGGVSVSGLEMSQNSVRLQWTAEEV
FT                   DQKLRGIMRGIFVACRDTAKKYGHPKNYQMGANIAGFLKVADSMIEQGCV"
FT   polyA_signal    1581..1586
FT                   /gene="NADP-GDH"
XX
SQ   Sequence 1691 BP; 354 A; 498 C; 499 G; 340 T; 0 other;
     aagcttcacg tcgtcgttct cgtctccgct gtctgcatcc tttacttatc gcttatagaa        60
     tgtccacaaa ctaaaagtat accggtcaaa cttattctga gggttacgga acaagtgtgc       120
     gcgtagagag ccgggggctc ccgaggacct tcgggggaga tggctgttct tgaaaagctg       180
     accacaaata acgcctttaa ttacaggcgc cccagatttt aaaatgcctg cccagacgat       240
     cgaggagctc atcgcggtga tcaagcagag ggatggccac atgacggagt tccgccaggc       300
     cgtcgaggag gtcgtggact ccctcaaggt tatcttcgag cgcgagccga agtatatccc       360
     aatcttcgag aggatgcttg agccggagcg cgtcatcatc ttccgcgtgc cctggatgga       420
     tgacgctgga cgcatcaacg tcaaccgcgg cttccgtgtc cagtacaact ctgctctcgg       480
     cccctacaag ggtggcctcc gcttccaccc ctctgtcaat ctttcgattc tcaagttcct       540
     cggtttcgag cagatcctga agaactccct caccacgctc ccgatgggcg gcggcaaggg       600
     cggctccgac tttgacccaa agggcaagtc cgacaacgag gtcatgcgct tctgccagtc       660
     cttcatgacc gagctccaga ggcacgtcgg cgccgacact gacgttcctg ccggcgacat       720
     cggcgtcggc gcccgcgaga tcgggtacct gtacggacag tacaagcgcc tgaggaacga       780
     gttcacaggc gtcctcacag gcaagaacgt caagtggggc gggtctttca tcaggccgga       840
     ggccacgggc tatggcgctg tctacttcct ggaggagatg tgcaaggaca acaacactgt       900
     gatcaggggt aagaacgtcc ttctttctgg ctccggcaac gttgcccagt ttgcttgcga       960
     gaagctcatt cagctcggcg caaaggtcct caccttctca gactccaacg ggaccattgt      1020
     cgacaaggac gggttcaacg aggagaagct ggcccacctc atgtacctca agaacgagaa      1080
     gcgtgggcgt gtttccgagt tcaaggacaa gtatcccagc gtcgcgtact acgagggcaa      1140
     gaagccttgg gagtgcttcg agggccagat ggattgcatc atgccttgcg ccactcagaa      1200
     cgaggtttcc ggggacgatg cgacgcgcct ggtcggcctc ggcctcaagt tcgtggccga      1260
     gggtgcgaac atgccctcca cggcagaggc tgttcacgtc tatcacgcca agggcgtgat      1320
     gtacggaccc gccaaggcca gcaacgcggg cggtgtctct gtctccggcc tcgagatgtc      1380
     ccagaattcc gtgaggctcc agtggacggc tgaggaggtc gaccagaagc tccgcggcat      1440
     catgaggggc atcttcgtcg cctgccgcga cactgccaag aagtatgggc accccaagaa      1500
     ctaccagatg ggcgcgaaca tcgccgggtt cctgaaggtc gcagactcta tgatcgagca      1560
     gggctgcgtg tgagggctga agtgaatatt taccttttcc gggttcgacg ggcgtgcttg      1620
     acgatgcgga agtcgctgtc cgttccaacg gctatctgcc ccaggacccc catcccggcg      1680
     ccgatcagct g                                                           1691
//
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