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EBI DbfetchID M76588; SV 1; linear; genomic DNA; STD; PRO; 2831 BP. XX AC M76588; XX DT 13-SEP-1991 (Rel. 29, Created) DT 17-APR-2005 (Rel. 83, Last updated, Version 5) XX DE Bacillus lautus endo-beta-1,4-glucanase (celA) gene, complete cds. XX KW celA gene; endo-beta-1,4-glucanase. XX OS Paenibacillus lautus OC Bacteria; Firmicutes; Bacillales; Paenibacillaceae; Paenibacillus. XX RN [1] RP 1-2831 RX PUBMED; 1592807. RA Hansen C.K., Diderichsen B., Jorgensen P.L.; RT "celA from Bacillus lautus PL236 encodes a novel cellulose-binding RT endo-beta-1,4-glucanase"; RL J. Bacteriol. 174(11):3522-3531(1992). XX FH Key Location/Qualifiers FH FT source 1..2831 FT /organism="Paenibacillus lautus" FT /strain="PL236" FT /mol_type="genomic DNA" FT /db_xref="taxon:1401" FT CDS <1..438 FT /codon_start=1 FT /transl_table=11 FT /note="ORF" FT /db_xref="GOA:P29718" FT /db_xref="HSSP:1NBC" FT /db_xref="InterPro:IPR001956" FT /db_xref="UniProtKB/Swiss-Prot:P29718" FT /protein_id="AAA22302.1" FT /translation="LQYRAADTNAADNQIKPSFNIKNNGTSAVDLSTLKIRYYFTKDGS FT AAVNGWIDWAQLGGSNIQISFGNHTGTNSDTYVELSFSSEAGSIAAGGQSGETQLRMSK FT TDWSNFNEANDYSFDGTKTAFADWDRVVLYQNGQIVWGTAP" FT RBS 518..522 FT /gene="celA" FT CDS 531..2633 FT /codon_start=1 FT /transl_table=11 FT /gene="celA" FT /product="endo-beta-1,4-glucanase" FT /EC_number="3.2.1.4" FT /db_xref="GOA:P29719" FT /db_xref="HSSP:1NBC" FT /db_xref="InterPro:IPR001956" FT /db_xref="UniProtKB/Swiss-Prot:P29719" FT /protein_id="AAA22303.1" FT /translation="MKTRQRKRLFVSAALAVSLTMTVPMPASVNAAASDVTFTINTQSE FT RAAISPNIYGTNQDLSGTENWSSRRLGGNRLTGYNWENNASSAGRDWLHYSDDFLCGNG FT GVPDTDCDKPGAVVTAFHDKSLENGAYSIVTLQMAGYVSRDKNGPVDESETAPSPRWDK FT VEFAKNAPFSLQPHLNDGQVYMDEEVNFLVNRYGNASTSTGIKAYSLDNEPALWSETHP FT RIHPEQLQAAELVAKSIDLSKAVKNVDPHAEIFGPALYGFGAYLSLQDAPGWPSLQGNY FT SWFIDYYLDQMKNAHTQNGKRLLDVLDVHWYPEAQGGGQRIVFGGAGNIDTQKARVQAP FT RSLWDPAYQEDSWIGTWFSSYLPLIPKLQSSIQTYYPGTKLAITESSYGGDNHISGGIA FT TADALGIFGKYGVYAANYWQTEDNTDYTSAAYKLYRNYDGNKSGFGSIKVDAATSDTEN FT SSVYASVTDEENSELHLIVLNKNFDDPINATFQLSGDKTYTSGRVWGFDQTGSDITEQA FT AITNINNNQFTYTLPPLSAYHIVLKADSTEPVNSDLVVQYKDGDRNNATDNQIKPHFNI FT QNKGTSPVDLSSLTLRYYFTKDSSAAMNGWIDWAKLGGSNIQISFGNHNGADSDTYAEL FT GFSSGAGSIAEGGQSGEIQLRMSKADWSNFNEANDYSFDGAKTAYIDWDRVTLYQDGQL FT VWGIEP" FT sig_peptide 531..629 FT /gene="celA" FT mat_peptide 630..2630 FT /gene="celA" FT /product="endo-beta-1,4-glucanase" XX SQ Sequence 2831 BP; 742 A; 700 C; 784 G; 605 T; 0 other; ctgcagtaca gagcggccga tacaaatgca gccgacaacc agatcaagcc gtccttcaac 60 atcaaaaaca acggtacttc ggctgttgat ttaagcacgc tcaaaatccg ctactacttc 120 accaaggatg gttctgcggc ggtgaacggc tggatcgact gggcgcagct cggcggcagc 180 aacattcaga tctcgtttgg caaccatact ggcacgaatt cggatacgta cgtggagctg 240 agcttctcgt ccgaggcagg ctcgattgcg gcgggcggcc aatccggtga aacccagctg 300 cgcatgtcca agacggactg gtcgaacttt aacgaggcga acgactactc gttcgatggg 360 acgaagacgg cctttgctga ctgggatcgg gtcgtattgt accagaacgg ccaaatagtg 420 tggggaactg ctccataaac cgatacaggg gaatgtgccg gaaccgctct tttgcagggc 480 agactggcgg tatcccttgc tgaaatgact attcctggga gggatcaaaa atgaagacaa 540 gacaaagaaa gcggctgttc gtcagtgcgg cgctggcagt atccttgaca atgaccgtac 600 cgatgcccgc ttctgtaaat gcagctgcga gtgatgtcac tttcacgatt aatacgcagt 660 cggaacgtgc agcgatcagc cccaatattt acggaaccaa tcaggatctg agcgggacgg 720 agaactggtc atcccgcagg ctcggaggca accggctgac gggttacaac tgggagaaca 780 acgcatccag cgccggaagg gactggcttc attacagcga tgattttctc tgcggcaacg 840 gtggtgttcc agacaccgac tgcgacaagc cgggggcggt tgttaccgct tttcacgata 900 aatctttgga gaatggagct tactccattg taacgctgca aatggcgggt tatgtgtccc 960 gggataagaa cggtccagtt gacgagagtg agacggctcc gtcaccgcgt tgggataagg 1020 tcgagtttgc caaaaatgcg ccgttctccc ttcagcctca tctgaacgac ggacaagtgt 1080 atatggatga agaagttaac ttcctggtca accggtatgg aaacgcttca acgtcaacgg 1140 gcatcaaagc gtattcgctg gataacgagc cggcgctgtg gtctgagacg catccaagga 1200 ttcatccgga gcagttacaa gcggcagaac tcgtcgctaa gagcatcgac ttgtcaaagg 1260 cggtgaagaa cgtcgatccg catgccgaaa tattcggtcc tgccctttac ggtttcggcg 1320 catatttgtc tctgcaggac gcaccgggtt ggccgagttt gcaaggcaac tacagctggt 1380 ttatcgatta ctatctggat cagatgaaga atgctcatac gcagaacggc aaaagattgc 1440 tcgatgtgct ggacgtccac tggtatccgg aagcacaggg cggaggccag cgaatcgtct 1500 ttggcggggc gggcaatatc gatacgcaga aggctcgcgt acaagcgcca agatcgctat 1560 gggatccggc ttaccaggaa gacagctgga tcggcacatg gttttcaagc tacttgccct 1620 taattccgaa gctgcaatct tcgattcaga cgtattatcc gggtacgaag ctggcgatca 1680 cagagtccag ctacggcgga gacaatcaca tttcgggagg catagctacc gcggacgcgc 1740 tcggcatttt tggaaaatat ggcgtttatg ccgcgaatta ctggcagacg gaggacaata 1800 ccgattatac cagcgctgct tacaagctgt atcgcaacta cgacggcaat aaatcggggt 1860 tcggctcgat caaagtggac gccgctacgt ccgatacgga gaacagctcg gtatacgctt 1920 cggtaactga cgaggagaat tccgaactcc acctgatcgt gctgaataaa aatttcgacg 1980 atccgatcaa cgctactttc cagctgtctg gtgataaaac ctacacatcc gggagagtat 2040 ggggcttcga ccaaaccgga tccgacatta cggaacaagc agctataacg aatattaaca 2100 acaatcaatt cacgtatacg cttcctccat tgtcggctta ccacattgtt ctgaaagcgg 2160 atagcaccga accggtcaac tccgatctcg tcgtgcagta taaggacggt gatcgcaaca 2220 atgcaaccga caatcagatc aagccgcatt tcaatattca aaataaaggg accagcccgg 2280 tagatctgag ttcccttacc ctgcgctact attttaccaa agacagctct gcagcgatga 2340 acggctggat cgattgggcg aagctcggcg gcagcaacat tcagatttcg ttcggtaatc 2400 ataatggcgc ggattcggat acgtacgcgg agctgggctt ctcgtccggc gcaggctcga 2460 ttgcggaggg cggtcaatcc ggcgaaatcc agctgcgcat gtcgaaggcg gactggtcga 2520 acttcaacga ggcgaacgac tactcgttcg atggggcgaa gacggcctat atagattggg 2580 atcgcgtgac gctataccaa gacggacaac tcgtatgggg aatcgagccg tagaagatga 2640 ctagacaaca ttagtgatga gacgcggccg gccataacgg ctgtcttgac tctgattcga 2700 tcaaaaaatc aaagcaaagg ggatgaaagt aatgaatgtt gcgattcaaa agagaatcgg 2760 atcaatattg atgattgcct cactaattat tagcttattg ccgttaggga gcagcagaac 2820 gtatgctgca g 2831 // |
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