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EBI DbfetchID M76435; SV 1; linear; genomic DNA; STD; PRO; 2316 BP. XX AC M76435; XX DT 09-DEC-1991 (Rel. 30, Created) DT 17-APR-2005 (Rel. 83, Last updated, Version 4) XX DE K.pneumoniae KpnI restriction endonuclease and DNA methylase genes, DE complete cds. XX KW DNA methylase; restriction endonuclease. XX OS Klebsiella pneumoniae OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacteriales; OC Enterobacteriaceae; Klebsiella. XX RN [1] RP 1-2316 RX DOI; 10.1093/nar/19.23.6505 RX PUBMED; 1754388. RA Chatterjee D.K., Hammond A.W., Blakesley R.W., Adams S.M., Gerard G.F.; RT "Genetic organization of the KpnI restriction--modification system"; RL Nucleic Acids Res. 19(23):6505-6509(1991). XX FH Key Location/Qualifiers FH FT source 1..2316 FT /organism="Klebsiella pneumoniae" FT /mol_type="genomic DNA" FT /db_xref="taxon:573" FT CDS complement(182..838) FT /codon_start=1 FT /transl_table=11 FT /standard_name="KpnI restriction endonuclease" FT /product="restriction endonuclease" FT /db_xref="GOA:P25237" FT /db_xref="UniProtKB/Swiss-Prot:P25237" FT /experiment="experimental evidence, no additional details FT recorded" FT /protein_id="AAA25089.1" FT /translation="MDVFDKVYSDDNNSYDQKTVSQRIEALFLNNLGKVVTRQQIIRAA FT TDPKTGKQPENWHQRLSELRTDKGYTILSWRDMKVLAPQEYIMPHATRRPKAAKRVLPT FT KETWEQVLDRANYSCEWQEDGQHCGLVEGDIDPIGGGTVKLTPDHMTPHSIDPATDVND FT PKMWQALCGRHQVMKKNYWDSNNGKINVIGILQSVNEKQKNDALEFLLNYYGLKR" FT gene complement(844..851) FT /gene="restriction endonuclease" FT RBS complement(844..851) FT /gene="restriction endonuclease" FT gene 996..1001 FT /gene="DNA methylase" FT RBS 996..1001 FT /gene="DNA methylase" FT CDS 1006..2259 FT /codon_start=1 FT /transl_table=11 FT /standard_name="KpnI methylase" FT /product="DNA methylase" FT /note="putative" FT /db_xref="GOA:P25238" FT /db_xref="HSSP:1G60" FT /db_xref="InterPro:IPR002052" FT /db_xref="UniProtKB/Swiss-Prot:P25238" FT /protein_id="AAA25090.1" FT /translation="MDNHANEINKLSRELGLLSNYEFNMDELKNLSPLDSTSSSIYIGD FT NLTYLQGLSKTSPKTIDFCYIDPPYNTGNKIIYHDNRKSVSSDIFGLHNEWMSFLLPRL FT FHAHKMLKDTGIIAISIDDYEFAHLKILMDKIFGEDNFIGNIVVCRSKNGKGSKRNIAS FT AHEYLLVYGKSDMAELSGQPDDKSLYDKVDCFGEYRIDGMFRKKGDSSLRTDRPNMFYP FT LYFNPSTGEVQVEPELGLKTVYPIDSKGIERRWLWSKETARERSWELFASKNGVVYVKN FT YSSSHKRIKVRTLWNDSSFYTERATNEITKIFGSKVFDTPKALNYIMSIINCMAKPDAL FT ILDFFAGSGTTAHAAAVLNSLDGGSRKTILMESNHPITKTHIAYKSGFRKISDITISRL FT NYVSDNFPDFKYKKIEII" XX SQ Sequence 2316 BP; 757 A; 405 C; 387 G; 767 T; 0 other; cccgagtata tgcatatgtt ttcattacca cctccttttc ctattatata ctaaaagtga 60 gatgtgtaaa taaaataaat tatttgatgg cattaaaagt gataaaaagc tacacatatc 120 cctagctcgt ttagagagga agggatatat gatagtatta gtgaagtttg ttaatattta 180 attatctttt caatccataa taattcaaaa gaaactctaa agcatcattc ttttgtttct 240 catttactga ctgcaatata ccaatgacat ttattttccc attatttgaa tcccaataat 300 tttttttcat aacttgatga cgtccacaca atgcttgcca cattttagga tcatttacat 360 cagttgcggg atctattgaa tgaggtgtca tatggtctgg tgttagtttg accgtgcctc 420 cccctatagg atcaatatca ccttcaacta acccacagtg ttgaccatct tcctgccact 480 cgcaagagta attagctcta tccaaaacct gttcccaggt ttcttttgtc ggtaatacac 540 gctttgctgc ctttgggcgt cttgttgcgt gtggcattat atactcttgc ggagctaaaa 600 ccttcatatc ccgccaggat aaaatagtat atcctttatc agttcgtagt tctgaaagtc 660 tctgatgcca attttctggt tgtttccctg tttttggatc agttgccgcc ctaatgattt 720 gctgacgagt tacaactttg ccaaggttat taagaaatag ggcttcaata cgctgactta 780 cagttttttg gtcataacta ttattatcat cactataaac tttatcaaag acatccattt 840 gatacctcct ttcattaacc atcaaacatt acattgccac attttcatta aattttaaag 900 ggaattctgc aatttataaa tcacatcata attgaataaa atttcaacat ggctattttc 960 aatgattatc acttattgta taatataaaa ataataggag ggtacatgga taatcatgct 1020 aacgaaataa ataaactaag ccgcgagctt ggccttttat caaattacga attcaatatg 1080 gatgaactta aaaatttatc gccattggat tctactagct catcaattta tataggtgat 1140 aatcttacct accttcaggg actttcaaaa accagtccaa agaccattga tttttgctat 1200 attgatcctc cttacaatac aggcaataaa atcatatatc atgataaccg gaaatcagtt 1260 tcctctgata tttttggttt acataacgaa tggatgtcat ttttgcttcc aagacttttt 1320 catgcacata aaatgctaaa agatacaggc attattgcta tcagcattga tgactatgaa 1380 tttgctcatt taaaaatact gatggataaa attttcggtg aagataattt catcggaaat 1440 atcgtcgttt gtcgttcaaa aaatggaaaa ggcagcaagc gaaatatagc gtctgctcat 1500 gaatatttac tggtttatgg aaaatcagat atggcggaac tatctggaca accagatgat 1560 aaatctcttt atgataaagt tgattgtttt ggtgaatata gaattgacgg aatgttcaga 1620 aaaaaaggtg attcaagttt gagaactgat cgccctaata tgttttatcc tttatatttt 1680 aacccatcaa caggtgaagt acaggtagag ccagaacttg gtttaaaaac agtctatcca 1740 atcgattcaa aagggattga acggagatgg ttatggagta aagaaacagc acgagaacgc 1800 tcttgggaat tgtttgctag caagaacggt gtagtttatg ttaaaaacta ttcatcttct 1860 cataaacgga ttaaagtcag aactctctgg aatgatagtt ctttttatac tgagagagcg 1920 acgaacgaaa ttacaaaaat atttggctca aaggtatttg atacccctaa agccctaaat 1980 tatattatgt ctataattaa ttgcatggca aaacctgacg cattaatcct tgatttcttt 2040 gccggttccg gaacgactgc tcatgctgcg gctgttctaa atagcctaga tggtggttca 2100 agaaaaacga tcttaatgga aagcaatcat ccgattacta agacacacat tgcatataaa 2160 tccggcttta gaaaaatatc ggatataacc atatcgagat tgaattatgt ttcggataat 2220 tttccggatt ttaaatataa aaaaatagaa ataatatagc aaatatttaa aattaagccg 2280 acggtgagat cacgctcggt ttgtaatgaa aagctt 2316 // |
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