 |
|
EBI DbfetchID M74821; SV 1; linear; genomic DNA; STD; PRO; 5776 BP. XX AC M74821; XX DT 24-AUG-1991 (Rel. 29, Created) DT 04-MAR-2000 (Rel. 63, Last updated, Version 3) XX DE E.coli restriction endonuclease (eco57IR) and methylotransferase (eco57IM) DE genes, complete cds. XX KW eco571M gene; eco571R gene; methylotransferase; restriction endonuclease. XX OS Escherichia coli OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacteriales; OC Enterobacteriaceae; Escherichia. XX RN [1] RP 1-5776 RA Janulaitis A., Vaisvila R., Timinskas A., Klimasauskas S., Butkus V.; RT "Cloning and sequence determination of the genes coding for Eco57I type IV RT restriction - modification enzymes"; RL Unpublished. XX FH Key Location/Qualifiers FH FT source 1..5776 FT /organism="Escherichia coli" FT /strain="RFL57" FT /mol_type="genomic DNA" FT /db_xref="taxon:562" FT -35_signal 634..640 FT /gene="eco57IM" FT TATA_signal 657..662 FT /gene="eco57IM" FT RBS 687..690 FT /gene="eco57IM" FT CDS 696..884 FT /codon_start=1 FT /transl_table=11 FT /gene="eco57IM" FT /note="ORF S" FT /db_xref="UniProtKB/Swiss-Prot:P25287" FT /protein_id="AAA23387.1" FT /translation="MAKGESERIVLEVEPELKKALYSVLAMEQKTLKDWFVDKAQEHIC FT EKKSELIERFSKVDNEI" FT CDS 874..2508 FT /codon_start=1 FT /transl_table=11 FT /gene="eco57IM" FT /product="methyltransferase" FT /db_xref="GOA:P25240" FT /db_xref="InterPro:IPR002052" FT /db_xref="InterPro:IPR002296" FT /db_xref="UniProtKB/Swiss-Prot:P25240" FT /protein_id="AAA23388.1" FT /translation="MKFKADQTSQKLRGGYYTPQNLADYVTKWVLSKNPKTILEPSCGD FT GVFIQAIANNGYNSNIELFCFELFDTEASKALERCKLNNFSNATITEGDFLVWANECLK FT KNKQIFDGALGNPPFIRYQFLERNFQEQAQLVFEHLDLKFTKHTNAWVPFLLSSLALLK FT QGGRIGMVIPSEISHVMHAQSLRSYLGHVCSKIVIIDPKEIWFEDTLQGAVILLAEKKQ FT YPDEASQGVGIVSVSGFEFLQEDPNVLFNDTAGINGETVEGKWTKATLSIDELQLIKRV FT IAHPDVRKFKDIAKVDVGRYCDGANNYFLVDNETVKLYKLERFAHPMFGRSQHCPGIIY FT DEKQHIENQEKGLPTNFLYIDEEFEYLSKSVKNYIKLGEVEEYHKRYKCRIRKPWFKVP FT SVYSTEIGMLKRCHDAPRLIHNRVRAYTTDTAYRVSSTVTSTENLVCSFLNPITVITAE FT LEGLFYGGGVLELVPSEIEKLYILIVEGLEHNVEELNLLIKDGQIERVIRQQGSLILGT FT LGFTQEENEKLVEIGRSLEIEGYVSRV" FT CDS complement(2570..5563) FT /codon_start=1 FT /transl_table=11 FT /gene="eco57IR" FT /product="restriction endonuclease" FT /db_xref="GOA:P25239" FT /db_xref="InterPro:IPR002052" FT /db_xref="InterPro:IPR002296" FT /db_xref="InterPro:IPR007409" FT /db_xref="InterPro:IPR011639" FT /db_xref="UniProtKB/Swiss-Prot:P25239" FT /protein_id="AAA23389.1" FT /translation="MNKKDQLQRLIDKYKSDIDYYRSARYNETQLRTDFLDQLFLLLGW FT DITNAAGKPTNEREVLVEEGLKARAGENTKKPDYTFRLFSERKFFLEAKKPSVDISTTI FT EPALQVRRYGFTAKLKISVLSNFEYTAIYDCSNQVKETDSVANSRIKLYHFTELVDKFD FT EINNLIGRESVYTGHFDNEWSEIENKILRFSVDDLFLKQINDWGLLLANEFLQIKNELP FT EEKLNDLVQNYINSIVFLRVCEDRDLEEYETLYHFAQDKDFQSLVKKLKSSDKKYNSGL FT FSLEYIDELLSNANSCIEHHRTIILPHKAHTLSLFSLLIFLGNIYEIFLSEKVRIDELG FT NVKIQPKEEHIDRDVVTTPTHIVKEIIRNTVVEYCKGKSDIEILNSKFADIACGSGAFI FT IVAFQFIQDILIDYYIQNDKSKLQQISEHTYKLKFEVKREILCKCIYGIDKDYNATKAC FT TFGLLLKLLEGETTETIGKDTPILPALDTNILFGNSLIDSGDKVKQEDIFSINPFDLTN FT YQFDVIVGNPPYMATEHMNQLTPKELDIYKRKYKSAYKQFDKYFLFIERSIQILKEYGY FT LGYILPSRFIKVDAGKKLRKFLSENKYLSKLISFGSHQVFKNKTTYTCLLFLNKENHDN FT FSFYEVKDNKKWLTREDKYLLSSTYQTSSLDSDTWVLEKKINDILKLMFSKSEQLGNIV FT GKSNVANGIQTSANKYYIHKEIKSENGFIYFEYDGIEYHIEKELTRPYFETNRSGDDSF FT YTYKDVEPNSFVVYPYKKVGERIQFIEYDELKRQYPKLFEFLQVVKVHLNDKKRSIKPD FT PTGLMMVQIRRSQALENCDVDQKLIVGILSNGYKYSIDNHRTFVSSGGTAGYSIINIPN FT NVRYSIYYIQAILTSKYLEWFASIYGDIFRGRFVARGTKVQTRMPIPTIDFDDPKQKEI FT HDTISSKQQYLNKLYSQTQKSADRDKIIFERQFEQEKIQMDYLIKNLFDLGDLDSEIPT FT VEDLYKNL" FT RBS complement(5570..5573) FT /gene="eco57IR" FT TATA_signal complement(5606..5612) FT /gene="eco57IR" FT -35_signal complement(5640..5645) FT /gene="eco57IR" XX SQ Sequence 5776 BP; 1831 A; 983 C; 1031 G; 1931 T; 0 other; taaagtgtgg ttttaggccg aatttcaatt gattcatcat ttgtggaatg taactaatcg 60 acatgcttcc caaacagaaa aatcgcttct attcccaaca gaaattttac gaacatgagc 120 gcaattattt ctctgctagg ataattggtt tttagtaaat cgctacagca ggcaattgcc 180 aatataaaaa tctggcacaa gggtgaacag cgcgcgccac ataatccatt gttattgcta 240 tatattagcg gaatacataa atgaacagcc acgtcttttc gattatggtc gaaaatctac 300 gaacctctgc acagtttacg tagaactttt tttggccaca gagttcacag taccggccta 360 tatcgtggcg cttgaaggaa gatagagaaa gattaaagat ggtggatatt gagttatgca 420 aaactgcaac ttggataatg cagtgctact gcggcccgat tttggcagta tctttagtat 480 attcagagcg gtatagcctt acacttttat tccgttcggc tttactgcta agatatatgg 540 ctctgcagag ccttcccctt accatattgg ctagggattc ttctgacttt tcaaacggtt 600 gaaaagaatg ctgaaaggca agccagtttt ttctttagaa gctctgaaaa cccatgtatt 660 atatgtaaca taacatataa tacatgggtg ttaatatggc aaaaggtgaa tcagaaagaa 720 tcgtcctaga ggttgagcca gaactaaaaa aagctctcta ttcagttctt gcaatggaac 780 aaaaaactct taaagactgg tttgttgata aggctcaaga acatatatgc gagaaaaaat 840 cagagctcat agagagattt tcgaaggtag acaatgaaat ttaaagcaga tcaaacctca 900 caaaaactta gaggtggcta ttacacccca caaaatttag ctgattatgt aactaaatgg 960 gtcttaagta agaatcctaa aaccatactt gagccgagtt gtggtgatgg tgtgtttatt 1020 caggctattg ctaataatgg ttataactct aacattgagt tattttgctt tgaattgttc 1080 gacactgaag catctaaggc tctcgaacgc tgtaaactga acaatttttc taatgcaacg 1140 attactgagg gggattttct agtctgggct aatgaatgtc ttaagaaaaa taagcagata 1200 tttgatggag ctttaggtaa tcctccattc atccgttatc agttccttga aagaaatttc 1260 caagaacaag ctcagttggt cttcgaacat ttagatctaa aatttacaaa gcacactaat 1320 gcttgggttc cattcttact ttcttcatta gcccttctca aacaaggtgg aagaataggg 1380 atggtcatac catcggagat tagtcatgta atgcacgctc aatcattgag aagctactta 1440 ggtcatgttt gctctaagat tgtcatcatt gaccctaaag agatttggtt tgaagataca 1500 ttacaggggg ccgttattct ccttgcagag aaaaagcaat atccagatga agcatcgcaa 1560 ggtgtaggta ttgttagtgt tagtggtttt gaattccttc aagaagatcc aaacgttctg 1620 tttaatgata cggcaggaat aaatggtgaa acagttgaag gtaaatggac taaagcaaca 1680 ctcagtattg acgagcttca gctaattaaa cgggtaattg ctcatccaga tgttcgtaaa 1740 tttaaagata tagctaaagt tgatgtaggt aggtattgtg acggggccaa taattatttt 1800 cttgttgata atgaaacagt taaattatat aaattagaac gttttgctca ccctatgttt 1860 ggcagaagcc aacattgccc cggaattatt tatgatgaga agcagcacat tgaaaaccag 1920 gaaaagggtt taccaaccaa ttttttatat atagatgaag agtttgaata tctttctaaa 1980 agtgtcaaaa attacataaa gttaggtgaa gtagaggaat accacaagcg ttataagtgc 2040 cgtatacgta agccttggtt caaggtccca tctgtatata gcacagagat tggaatgtta 2100 aagcgttgtc acgatgcgcc acgcttaatt cataacagag ttagagcata taccactgat 2160 actgcatata gagtttcttc aacagttact agtactgaga acttagtgtg tagttttttg 2220 aaccctataa cggtcattac agccgaactc gaaggactct tttacggcgg tggcgttctt 2280 gaacttgtcc catccgagat agaaaaacta tatatcctta tcgttgaagg attagaacat 2340 aacgttgaag aacttaacct attgattaaa gatggtcaaa tagagagggt aattcgacaa 2400 caaggttcac taatcttagg cacgcttggt ttcactcaag aagaaaatga aaaacttgta 2460 gaaataggaa gaagcttaga gatagaaggc tacgtaagta gggtttaaaa agtcgggggc 2520 tcataaaaaa tcagtgcagc ttttagctca ttgattttat ctggtaacct tataggtttt 2580 tgtatagatc ttctactgtt ggaatctcgg aatctaaatc ccccaagtca aaaagattct 2640 taatcaaata atccatttgg attttttctt gttcaaattg cctttcgaat atgattttat 2700 ctctatcagc tgatttttga gtttggctat acagtttatt caaatactgt tgctttgaac 2760 tgatcgtatc atgtatttct ttctgttttg gatcatcaaa atcaatagta ggaataggca 2820 ttctagtttg aacttttgtc ccacgagcaa caaatctacc tctaaaaata tcaccatata 2880 ttgaagcaaa ccactcaagg tactttgatg taaggattgc ttggatataa tagatagagt 2940 atctaacgtt attaggaatg ttgataatgc tatagcccgc agtgcctcca gaagaaacaa 3000 aagttctatg gttatcaatg ctgtacttat atccattaga tagaatacca acaatcagct 3060 tctgatcaac atcgcagttt tctaatgctt ggctacgccg tatctgtacc atcattaggc 3120 cagttgggtc aggcttaata gagcgtttct tatcattcaa atggactttt acaacctgca 3180 ggaactcaaa taacttagga tattgacgtt ttagttcatc gtattcaatg aactggattc 3240 tttcacccac tttcttgtat gggtaaacaa caaatgaatt aggttcaacg tctttataag 3300 tatagaaact atcatcacct gaacgattcg tttcgaagta agggcgagtt aattcctttt 3360 caatgtggta ttcaattcca tcatattcaa aataaataaa accattttca gatttgattt 3420 ctttatgaat gtagtattta tttgcacttg tctgaatgcc atttgcaaca ttactttttc 3480 ctacaatgtt acctagctgt tcagattttg aaaacattag cttcaaaatg tcattgattt 3540 tcttttctag aacccaagtg tcagaatcaa gagaactggt ttgatatgtg cttgataata 3600 aatatttatc ttcacgagtt aaccacttct tgttgtcttt tacttcataa aaagaaaagt 3660 tatcgtgatt ttctttattt aaaaataaca aacaagtgta agtcgtttta ttcttgaata 3720 cttgatgaga accaaaagaa ataagtttag ataaatattt attttctgat aaaaacttgc 3780 gtagtttttt acctgcatct acttttatga acctagacgg aagaatatag cctaaatagc 3840 cgtactcttt aagaatttgt attgaacgct caataaataa aaagtattta tcgaattgct 3900 tataagcaga tttatatttc ctcttgtaaa tatctaattc ctttggtgtt aactgattca 3960 tatgctcagt cgccatataa ggaggatttc caacgatcac atcaaattgg tagttagtca 4020 aatcaaatgg attaattgaa aaaatatctt cttgtttaac tttgtctcca ctatctatca 4080 agctattccc gaatagaatg ttcgtatcca gtgcaggcaa aataggggtg tctttaccaa 4140 tggtttctgt agtttcgcct tcgagaagct ttagcaataa accaaaggta caagcttttg 4200 ttgcattata gtctttatca atcccatata tacatttaca tagaatttct ctttttactt 4260 caaatttaag tttgtatgta tgttcagata tttgctgaag tttagatttg tcattttgta 4320 tataataatc aataagaata tcctggatga attggaatgc cacaataata aaagcacctg 4380 agccacaagc gatatcagca aattttgaat tcaatatctc aatatctgac ttgcctttgc 4440 aatattctac gacagtattt ctaatgattt cttttacaat atgagttggt gtagtaacaa 4500 cgtctctatc gatatgttcc tctttcggtt gaatcttaac gtttccaagt tcatcaatac 4560 gaactttctc actcaggaat atttcatata tattacctaa aaatatcaga agagaaaaca 4620 gagaaagagt atgtgctttg tggggaagta taattgttct atgatgctca atgcaagagt 4680 tagcattgct taataactca tcaatatatt ctagcgagaa taatcctgaa ttatatttct 4740 tgtctgagga tttcaatttt ttgactaaag attgaaaatc cttatcttgt gcaaagtgat 4800 aaagagtttc gtattcttcc aagtctctat cttcacagac tctaaggaaa acaatactgt 4860 taatataatt ttgtacaaga tcatttaatt tttcttctgg aagttcgttt tttatctgca 4920 aaaattcatt agcaagaagt aagccccaat cattaatttg cttgaggaat agatcatcaa 4980 cactgaatct cagtatctta ttttcaattt ctgaccattc attgtcaaaa tgaccagtgt 5040 aaacactttc tcgaccgata agattattaa tttcatcaaa cttatctacg agttcagtga 5100 agtgatacaa cttgattcta gagtttgcta ctgaatcagt ttctttaacc tgattagagc 5160 aatcataaat tgccgtatac tcgaaattcg acaacacaga aatttttaat ttggctgtga 5220 atccgtatcg tctaacttgt agagctggtt ctatagtagt tgatatatca acactaggct 5280 ttttggcttc aaggaaaaat ttacgctcag agaagagtct aaacgtgtag tctggctttt 5340 tagtattttc gcctgctcgg gcttttaaac cttcctctac aagcacttcg cgttcgtttg 5400 ttggttttcc tgctgcgttc gttatgtccc aaccgagaag aaggaatagt tgatctaaaa 5460 aatccgttct tagttgagtt tcgttgtatc tggcagaacg ataataatca atatcgcttt 5520 tgtatttatc aattagtctt tgtaactgat cttttttatt catctatttc tcacaacgat 5580 atttatcaaa aagtagattt ctaatgattg taattcttgt accgaagtat tgttagtcaa 5640 gttatcctaa cagttccatt ctttacgtct cttgttgtgc tgcacagatt ttgctgtcac 5700 tatttttacc tcaaaaacca cagtaacact accaataggt ggcttttcga tgatgactgc 5760 aaggaaagtg tggtat 5776 // |
|