 |
|
EBI DbfetchID M73326; SV 1; linear; unassigned DNA; STD; PRO; 4286 BP. XX AC M73326; XX DT 18-DEC-1991 (Rel. 30, Created) DT 14-NOV-2006 (Rel. 89, Last updated, Version 14) XX DE Escherichia coli phospho-beta-glucosidase (ascB), asc repressor (ascG), PTS DE enzyme II-asc (ascF) genes, complete cds. XX KW asc repressor; ascB gene; ascF gene; ascG gene; phospho-beta-glucosidase; KW PTS enzyme II-asc. XX OS Escherichia coli OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacteriales; OC Enterobacteriaceae; Escherichia. XX RN [1] RP 1-4286 RX PUBMED; 1630307. RA Hall B.G., Xu L.; RT "Nucleotide sequence, function, activation, and evolution of the cryptic RT asc operon of Escherichia coli K12"; RL Mol. Biol. Evol. 9(4):688-706(1992). XX FH Key Location/Qualifiers FH FT source 1..4286 FT /organism="Escherichia coli" FT /mol_type="unassigned DNA" FT /db_xref="taxon:562" FT CDS complement(126..1133) FT /codon_start=1 FT /transl_table=11 FT /gene="ascG" FT /product="asc repressor" FT /db_xref="GOA:P24242" FT /db_xref="InterPro:IPR000843" FT /db_xref="InterPro:IPR001761" FT /db_xref="InterPro:IPR010982" FT /db_xref="PDB:3BRQ" FT /db_xref="PDB:3DBI" FT /db_xref="UniProtKB/Swiss-Prot:P24242" FT /protein_id="AAA16428.1" FT /translation="MMTTMLEVAKRAGVSKATVSRVLSGNGYVSQETKDRVFQAVEESG FT YRPNLLARNLSAKSTQTLGLVVTNTLYHGIYFSELLFHAARMAEEKGRQLLLADGKHSA FT EEERQAIQYLLDLRCDAIMIYPRFLSVDEIDDIIDAHSQPIMVLNRRLRKNSSHSVWCD FT HKQTSFNAVAELINAGHQEIAFLTGSMDSPTSIERLAGYKDALASMVLRSMKNLSLTVN FT GRLPAGRRVEMLLERGAKFSALVASNDDMAIGAMKALHERGVAVPEQVSVIGFDDIAIA FT PYTVPALSSVKIPVTEMIQEIIGRLIFMLDGGDFSPKTFSGKLIRRDSLIAPSR" FT misc_feature 190 FT /gene="ascG" FT /label=IS186_insertion FT /note="IS186 insertion target site" FT RBS complement(1137..1142) FT /gene="ascG" FT promoter complement(1161..1189) FT /gene="ascG" FT promoter 1257..1286 FT /label=ascFB_promotor FT RBS 1377..1382 FT /gene="ascF" FT CDS 1390..2847 FT /codon_start=1 FT /transl_table=11 FT /gene="ascF" FT /product="PTS enzyme II-asc" FT /db_xref="GOA:P24241" FT /db_xref="InterPro:IPR001996" FT /db_xref="InterPro:IPR003352" FT /db_xref="InterPro:IPR004719" FT /db_xref="InterPro:IPR011535" FT /db_xref="InterPro:IPR013013" FT /db_xref="InterPro:IPR018113" FT /db_xref="UniProtKB/Swiss-Prot:P24241" FT /protein_id="AAA16429.1" FT /translation="MAKNYAALARSVIAALGGVDNISAVTHCMTRLRFVIKDDALIDSP FT TLKTIPGVLGVVRSDNQCQVIIGNTVSQAFQEVVSLLPGDMQPAQPVGKPKLTLRRIGA FT GILDALIGTMSPLIPAIIGGSMVKLLAMILEMSGVLTKGSPTLTILNVIGDGAFFFLPL FT MVAQSAAIKFKTNMSLAIAIAGVLVHPSFIELMAKAAQGEHVEFALIPVTAVKYTYTVI FT PALVMTWCLSYIERWVDSITPAVTKNFLKPMLIVLIAAPLAILLIGPIGIWIGSAISAL FT VYTIHGYLGWLSVAIMGALWPLLVMTGMHHVFTPTIIQTIAETGKEGMVMPSEIGANLS FT LGGSSLAVAWKTKNPELRQTALAAAASAIMAGISEPALYGVAIRLKRPLIASLISGFIC FT GAVAGMAGLASHSMAAPGLFTSVQFFDPANPMSIVWVFAVMALAVVLSFILTLLLGFED FT IPVEEAAAQARKYQSVQPTVAKEVSLN" FT RBS 2849..2852 FT /gene="ascB" FT CDS 2856..4280 FT /codon_start=1 FT /transl_table=11 FT /gene="ascB" FT /product="phospho-beta-glucosidase" FT /db_xref="GOA:P24240" FT /db_xref="InterPro:IPR001360" FT /db_xref="InterPro:IPR013781" FT /db_xref="InterPro:IPR017853" FT /db_xref="InterPro:IPR018120" FT /db_xref="UniProtKB/Swiss-Prot:P24240" FT /protein_id="AAA16430.1" FT /translation="MSVFPESFLWGGALAANQSEGAFREGDKGLTTVDMIPHGEHRMAV FT KLGLEKRFQLRDDEFYPSHEATDFYHRYKEDIALMAEMGFKVFRTSIAWSRLFPQGDEI FT TPNQQGIAFYRSVFEECKKYGIEPLVTLCHFDVPMHLVTEYGSWRNRKLVEFFSRYART FT CFEAFDGLVKYWLTFNEINIMLHSPFSGAGLVFEEGENQDQVKYQAAHHQLVASALATK FT IAHEVNPQNQVGCMLAGGNFYPYSCKPEDVWAALEKDRENLFFIDVQARGTYPAYSARV FT FREKGVTINKAPGDDEILKNTVDFVSFSYYASRCASAEMNANNSSAANVVKSLRNPYLQ FT VSDWGWGIDPLGLRITMNMMYDRYQKPLFLVENGLGAKDEFAANGEINDDYRISYLREH FT IRAMGGTIADGIPLMGYTTWGCIDLVSACTGEMSKRYGFVFVDRDDAGNGTLTRTHRKS FT FWWYKKVIASNGEDLE" XX SQ Sequence 4286 BP; 999 A; 1096 C; 1138 G; 1053 T; 0 other; aatgcgccga tacattcaca aatcatgcca gtttttaatt tactgttatt taaggaaatt 60 aatttctgta atgcaggaaa aacgatgtca tcgacactag tgacgatgac atgtgatgac 120 aatgtttatc gcgaaggagc aatgagtgag tcgcggcgga tcagttttcc gctgaaggtt 180 ttcggtgaga aatccccgcc atcgagcata aaaatcagcc gtccaataat ttcctgaatc 240 atctcagtca ccggaatttt tacgctggag agcgccggaa cggtgtaggg ggcaatagcg 300 atatcatcga atccgataac tgacacctgc tctggcaccg ctacgccgcg ctcgtgtaac 360 gctttcatcg cacctatcgc catatcgtcg ttactggcaa ctaacgcgct aaatttagcc 420 ccacgttcga gcaacatttc tacccttcgg cccgctggca ggcgtccatt taccgttagc 480 gataagtttt tcattgagcg caataccatg ctggccagcg cgtctttata cccggcaaga 540 cgttcaatgc tggtggggga atccatcgag ccggtaagga aagcaatctc ctgatgcccg 600 gcgtttatca actctgccac ggcgttaaaa ctggtctgtt tatgatcgca ccagacgcta 660 tggctgctgt ttttgcgcag gcggcgatta agcaccatta tcggctgact gtgcgcgtca 720 atgatgtcat cgatctcatc cacgcttaaa aaacgcgggt aaatcatgat cgcgtcgcag 780 cgcagatcca gcagatactg aatcgcctgg cgctcttctt ctgcgctgtg tttaccatct 840 gccaatagca actgccgccc tttctcttcc gccattcgcg cggcatgaaa gagtaattca 900 ctaaaataaa tgccgtggta aagcgtgttg gtcactacca gccccagcgt ctgagtactc 960 ttcgccgaca gattgcgcgc cagcaagttt ggacggtaac cgctctcttc taccgcctga 1020 aacacgcgat ctttagtctc ctggctgacg tagccattac ctgaaagcac gcgggaaacg 1080 gtcgcttttg aaaccccggc gcgcttcgcc acttccagca tcgtcgtcat cattttcatc 1140 cctttacacg caatcaacgc agtgtactgc accgtttgcg tattgtcctt gcacaatcgg 1200 cgggaaaaat attcaggtga ccggtttcac aaatataaaa aatgaacaat tcactctctt 1260 gcttatttag tgacaactat tcatgatttt gtgaaaccgg tttcttaatt ccgtttcagc 1320 atcggcattt ttccgtcacg tcgactgata acaactacat ctaccctact gataacagga 1380 taaaatccga tggccaaaaa ttatgcggcg ctggcacgct cggtgatagc ggcactgggc 1440 ggcgttgata acatctcggc ggtcacgcac tgtatgacgc ggttgcgctt tgttatcaaa 1500 gatgatgcac ttatcgacag cccgacgtta aaaaccatcc ccggcgtgct cggcgtggta 1560 cgtagtgaca accagtgtca ggtgattatc ggcaataccg tttcacaagc ctttcaggaa 1620 gtcgtcagcc tgctgccggg agatatgcag cccgcacagc ccgtgggtaa acccaaactc 1680 acgctacgtc gcattggtgc ggggatcctc gatgcgctga tcggcaccat gtcaccgctg 1740 atcccggcga ttatcggcgg atcgatggtc aaactgctgg caatgatcct cgagatgagc 1800 ggcgtgctga caaaaggatc gccgacctta accattctga atgtgattgg tgacggtgct 1860 ttcttcttcc tgccgctgat ggtcgcgcag tctgccgcca tcaaatttaa aaccaatatg 1920 tcgctggcga ttgccatcgc gggtgtgctg gtacatccga gttttattga actgatggcg 1980 aaagcggccc agggtgaaca tgtcgaattt gccctgattc cggtcaccgc ggtgaaatac 2040 acctacacgg tgatcccggc gctggtgatg acctggtgcc tgtcatatat cgaacgctgg 2100 gtggacagca ttacaccggc ggtgaccaaa aacttcctca aaccaatgct gattgtgttg 2160 attgccgcac cgctggcaat cctgctgatt ggcccgattg gtatctggat cggtagcgcc 2220 atttcggcgc tggtttacac cattcatggt tatctgggct ggctttcagt cgccattatg 2280 ggcgcgctgt ggcctctgct ggtaatgacc gggatgcacc acgtctttac gccaaccatc 2340 attcagacca ttgccgaaac cggcaaagaa gggatggtca tgccgtcaga gatcggcgct 2400 aacctgtcgc tgggcggttc atcactggca gtggcgtgga aaacgaaaaa cccggaactg 2460 cgtcagacgg cgctggctgc ggcggcatca gccattatgg cggggatttc cgaaccggcg 2520 ttatatggcg tggcgatccg cctgaaacgt ccgcttatcg ccagtcttat cagcggtttt 2580 atttgcggcg cggttgccgg tatggcgggg cttgccagcc actcaatggc agcgccgggg 2640 ctatttacca gcgtgcagtt cttcgatccg gcgaatccaa tgagcatcgt ctgggtgttc 2700 gcggtcatgg cgctggcggt ggtgctgtcg tttatcctca cactgttgct cggctttgag 2760 gatattcctg ttgaggaagc ggctgcccag gcgcgaaagt atcagagcgt acaaccgacc 2820 gtcgccaaag aagtaagtct taattgagga tgaaaatgtc agtatttcca gaaagttttt 2880 tatggggcgg cgcgcttgcc gccaaccagt ctgaaggtgc gttccgtgaa ggtgacaaag 2940 gtctgaccac tgtcgatatg atcccacacg gcgagcatcg aatggcggtg aaactggggc 3000 tggaaaaacg ttttcagttg cgagatgacg agttttatcc cagccatgag gcgacggatt 3060 tttatcatcg ttataaagaa gatatcgccc tgatggcaga gatgggattc aaggttttcc 3120 gtacctcaat tgcctggagc cgtctctttc cgcagggcga tgaaatcacg cccaatcagc 3180 agggcattgc tttttatcgt tctgtctttg aagagtgtaa aaagtacggt atcgaaccgc 3240 tggtcacgtt gtgccacttc gatgtgccga tgcatctggt caccgaatat ggctcctggc 3300 gtaaccgcaa gctggtggag tttttcagcc gctacgccag aacctgcttt gaagcatttg 3360 atggtctggt gaaatactgg ctaaccttca atgaaatcaa cattatgttg catagcccgt 3420 tctccggcgc gggtctggtg tttgaagaag gtgaaaatca ggatcaggtg aaatatcagg 3480 ccgcgcatca ccagctggtt gccagtgcgc tagccaccaa aatcgcccat gaggttaacc 3540 cgcaaaatca ggtgggctgt atgctggcgg gcggtaactt ctacccttac agttgcaagc 3600 cggaagatgt ctgggcggcg ctggagaaag accgggaaaa cctgtttttt atcgatgtgc 3660 aggcgcgggg cacgtatccg gcttactctg cccgcgtatt ccgcgaaaaa ggggtaacca 3720 tcaacaaagc accgggcgat gatgaaatcc tgaaaaacac cgtcgatttt gtctctttca 3780 gctattacgc ctcgcgctgc gcctcggcgg agatgaacgc caacaacagc agtgcggcga 3840 acgtggtgaa atcgctgcgt aatccgtatc tacaggtgag cgactggggc tggggaattg 3900 atccactcgg tctgcgtatc accatgaata tgatgtacga tcgttatcag aagccgctgt 3960 ttctggtgga aaacggcctg ggcgcaaaag atgaatttgc tgccaatggc gagattaacg 4020 acgactatcg catcagctac ttacgcgaac atatccgcgc aatgggcgga acgattgcag 4080 acggcattcc gctgatgggc tacaccacat ggggctgtat tgatttagtt tccgcctgta 4140 cgggtgaaat gagcaaacgc tacggctttg tctttgttga ccgtgacgac gcaggcaacg 4200 gtacgctgac gcgcactcac aggaaatcat tctggtggta taaaaaagtg attgccagta 4260 atggggaaga tttagagtag gtaaca 4286 // |
|