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EBI DbfetchID M66060; SV 1; linear; genomic DNA; STD; PRO; 5279 BP. XX AC M66060; XX DT 22-MAY-1991 (Rel. 28, Created) DT 14-NOV-2006 (Rel. 89, Last updated, Version 6) XX DE A.eutrophus protein X (acoX), acetoin:DCPIP oxidoreductase-alpha (acoA), DE acetoin:DCPIP oxidoreductase-beta (acoB), FMP (acoC), and ORF 7 genes, DE complete cds, and ORF 8, 5' end. XX KW acetoin catabolism; acetoin dehydrogenase; acetoin:DCPIP oxidoreductase; KW dihydrolipoamide acetyltransferase. XX OS Cupriavidus necator OC Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales; OC Burkholderiaceae; Cupriavidus. XX RN [1] RP 1-5279 RX PUBMED; 2061286. RA Priefert H., Hein S., Krueger N., Zeh K., Schmidt B., Steinbuechel A.; RT "Identification and molecular characterization of the Alcaligenes eutrophus RT H16 aco operon genes involved in acetoin catabolism"; RL J. Bacteriol. 173(13):4056-4071(1991). XX FH Key Location/Qualifiers FH FT source 1..5279 FT /organism="Cupriavidus necator" FT /strain="H16" FT /mol_type="genomic DNA" FT /db_xref="taxon:106590" FT promoter 53..69 FT /note="-24/-12 promoter" FT mRNA 82..5279 FT CDS 108..1187 FT /codon_start=1 FT /transl_table=11 FT /gene="acoX" FT /product="protein X" FT /db_xref="GOA:P27748" FT /db_xref="InterPro:IPR002504" FT /db_xref="InterPro:IPR011391" FT /db_xref="UniProtKB/Swiss-Prot:P27748" FT /experiment="experimental evidence, no additional details FT recorded" FT /protein_id="AAA21947.1" FT /translation="MGHAAGASAQIAPVVGIIANPISARDIRRVIANANSLQLADRVNI FT VLRLLAALASCGVERVLMMPDREGLRVMLARHLARRQGPDSGLPAVDYLDMPVTARVDD FT TLRAARCMADAGVAAIIVLGGDGTHRAVVRECGAVPIAGLSTGTNNAYPEMREPTIIGL FT ATGVYATGRIPPAQALASNKRLDIVIRDGNGGFRRDIALVDAVISHEHFIGARALWKTD FT TLAAVYVSFADPEAIGLSSIAGLLEPVGRREEGGLAIELAAPGEGEFDLCAPIAPGLMC FT TVPVAGWQRLEHGRPHRVRQRSGIVALDGERELAFGPDDEVTVTLHDHAFRSIDVAACM FT RHAGRHHLMRSLPQPAAVG" FT CDS 1259..2260 FT /codon_start=1 FT /transl_table=11 FT /gene="acoA" FT /product="acetoin:DCPIP oxidoreductase-alpha" FT /db_xref="GOA:P27745" FT /db_xref="InterPro:IPR001017" FT /db_xref="UniProtKB/Swiss-Prot:P27745" FT /experiment="experimental evidence, no additional details FT recorded" FT /protein_id="AAA21948.1" FT /translation="MTARASQDSAALPLDKETLLTVYRKMRTIRDFEERLHVDFGRGDI FT PGFVHLYAGEEAAGVGILHHLNDGDRIASTHRGHGHCIAKGVDPVAMMKEIYGKKGGSC FT NGKGGSMHIADLSKGMMGANGILGAGAPLICGAALAAKFRGKGEVGITFCGDGASNQGT FT FLESLNLAAVWNLPVIFVIENNGYAESTSRDYGTAVDSYVDRAAGFGIPGVTVDGTDFF FT AVHEAAGEVIRRAREGGGPSLLECKMVRFYGHFEGDAQTYRAAGELDDIRANKDCLKLF FT GRAVTQAGVVAREELDTIDREVAALIEHAVQEAKAAPQPGPEDLLTDVYVSY" FT CDS 2297..3313 FT /codon_start=1 FT /transl_table=11 FT /gene="acoB" FT /product="acetoin:DCPIP oxidoreductase-beta" FT /db_xref="GOA:P27746" FT /db_xref="InterPro:IPR005475" FT /db_xref="InterPro:IPR005476" FT /db_xref="InterPro:IPR009014" FT /db_xref="InterPro:IPR015941" FT /db_xref="UniProtKB/Swiss-Prot:P27746" FT /experiment="experimental evidence, no additional details FT recorded" FT /protein_id="AAA21949.1" FT /translation="MARKLSIKLAINEAIDQEMTRDPSVIMLGEDIVGGAGADGEKDAW FT GGVLGVTKGLYAKHGDRLLDTPLSESAYVGAAIGAAACGMRPIAELMFIDFMGVCFDQI FT FNQAAKFRYMFGGKAETPVVIRAMVGAGFRAAAQHSQMLTPLFTHIPGLKVVCPSTPYD FT TKGLLIQAIRDNDPVIFCEHKNLYGLEGEVPEGAYAIPFGEANIVRDGKDVSIVTYGLM FT VHRALEAAATLAKEGIEAEIVDLRTLSPLDMDTVLESVENTGRLVVVDEASPRCNIATD FT ISAQVAQQAFGALKAGIEMVCPPHTPVPFSPTLEDLYIPSAAQIAAAARKTMKGGKH" FT CDS 3313..4437 FT /codon_start=1 FT /transl_table=11 FT /gene="acoC" FT /product="FMP" FT /note="probably a dihydrolipoamide acetyltransferase" FT /db_xref="GOA:P27747" FT /db_xref="InterPro:IPR000073" FT /db_xref="InterPro:IPR000089" FT /db_xref="InterPro:IPR003016" FT /db_xref="InterPro:IPR011053" FT /db_xref="UniProtKB/Swiss-Prot:P27747" FT /experiment="experimental evidence, no additional details FT recorded" FT /protein_id="AAA21950.1" FT /translation="MATEISPTIIPIVMPKWGLSMKEGTVNAWLVDEGTEITVGLPILD FT VETDKIANAVEAPDAGTLRRKVAQAGDVLPVKALLGVLAPAEVSDAQIDGYVAAYETPA FT DDAGEEDAAAAYQFADGDGIRVRYARKGGGAETVLFIHGFGGDLDNWLFNLDPLADAYT FT VVALDLPGHGQSSPRLAGTTLAQMAGFVARFMDETGIEAAHVVGHSMGGGVAAQLAVDA FT PQRVLSVALVSPVGFGDAVNSGYTEGFVSAQSRRELKPVVELLFADAGLVSRQMLDDLL FT RYKRLDGVTEALTALGQGLFGGGRQSEQPGQRLANSGKRVLVVWGGQDQIIPAAHAEAA FT PPGATVKVFADAGHMSQMEKANDFNALLKKHLGG" FT CDS 4517..4858 FT /codon_start=1 FT /transl_table=11 FT /note="ORF 7" FT /db_xref="InterPro:IPR003779" FT /db_xref="InterPro:IPR004675" FT /db_xref="UniProtKB/Swiss-Prot:P27749" FT /experiment="experimental evidence, no additional details FT recorded" FT /protein_id="AAA21951.1" FT /translation="MTTDLKTRLTEINRQFGALGQAQPAAMSAFQGVMKAATQDGSLPA FT AVKELIAVALAVQKGCDDCVLFHTSQALRHRATREQLAEVLAINIEMGGGPGAMYASKA FT LAYFDALNA" FT CDS 4842..5279 FT /partial FT /codon_start=1 FT /transl_table=11 FT /note="ORF 8" FT /db_xref="UniProtKB/Swiss-Prot:P27750" FT /experiment="experimental evidence, no additional details FT recorded" FT /protein_id="AAA21952.1" FT /translation="MRSMPSVARLGTLLSGGWCHPMPFEFIDAAHAGQAGSDPLQDELA FT LLDLAAQGRQVAQLWEAPLSLVGARTYLRHAALETARADFAQQGCPVFLRMSGGGLVPQ FT GPGILNLSLAYTVEQPPGARSDAVYLHLCEVIGDALQSLGVD" XX SQ Sequence 5279 BP; 895 A; 1765 C; 1813 G; 806 T; 0 other; ggatccgacc ggctgatacc cctgtttccg ctgttttcac tgcctgcgcg cactggcaca 60 gccattgcaa atacgcctgg caagacaacg acaacaggag acaggcaatg gggcacgcag 120 caggcgccag cgcgcagatc gcgccagtgg tcggcatcat cgccaacccg atttccgcgc 180 gcgacatccg tcgtgtgatc gccaacgcca acagcctgca gctggccgac cgcgtcaata 240 tcgtgctgcg cctgctggcc gcgctggcct cctgcggcgt ggagcgcgtg ctgatgatgc 300 ccgaccgcga aggcctgcgc gtcatgctgg cgcggcacct ggcacgccgg caggggccgg 360 actcgggcct gcccgcggtc gactacctcg acatgccggt caccgcgcgc gtcgacgaca 420 ccctgcgcgc cgcgcgctgc atggccgatg cgggcgtggc cgcgatcatc gtgctgggcg 480 gcgacggcac ccaccgcgcg gtggtgcgcg agtgcggcgc ggtgccgatc gcgggcctgt 540 cgaccggcac caacaatgcc taccccgaaa tgcgcgagcc caccatcatc gggctggcaa 600 ccggcgtcta tgccacgggc cgcattcctc ctgcgcaggc gctggcgtcg aacaagcggc 660 tcgacatcgt catccgtgac ggcaacggcg gctttcgccg cgatattgcg ctggtggatg 720 cggtgatctc gcacgagcac ttcatcggcg cgcgcgccct gtggaagacc gacacgcttg 780 ccgccgtcta cgtgtccttt gccgaccccg aggccatcgg cctgtcatcc atcgccgggc 840 tgctggagcc ggtgggccgg cgtgaggagg gcggcctcgc gatcgaactg gccgcgcccg 900 gcgaaggcga attcgacctg tgcgcgccga tcgcacccgg cctgatgtgc accgtgccgg 960 tggccggctg gcagcggctc gaacacggcc ggccccatcg cgtgcgccag cgcagcggca 1020 ttgtcgcgct ggacggagag cgcgagctgg ccttcggccc ggacgacgaa gtcacggtca 1080 ccctgcatga ccacgccttt cgcagcatcg acgtcgcggc ctgcatgcgc catgccgggc 1140 gccaccacct gatgcgcagc ctgccgcagc ccgcggcggt gggatgacgc gcgggcaccg 1200 cgtccgcccg cttttcccct ttcttgaagt gcaacacctg aaaacgaagg agacagacat 1260 gacagccaga gcttctcaag attccgccgc gctgccgctc gacaaggaga cgttgctgac 1320 ggtgtaccgg aagatgcgca ccatccgcga tttcgaagaa cgcctgcacg tggacttcgg 1380 gcgcggcgac atcccgggct tcgtccatct atacgcgggc gaggaagccg cgggcgtcgg 1440 cattctgcac cacctgaacg atggcgaccg catcgccagc acacaccgcg gccacggcca 1500 ttgcattgcc aagggcgtcg atccggtggc gatgatgaag gagatctacg gcaagaaggg 1560 cggctcgtgc aatggcaagg gtggctcgat gcatatcgcc gacctgtcca agggcatgat 1620 gggcgccaac ggcatcctgg gcgcaggcgc gccgctgatc tgcggcgccg cgctggctgc 1680 caagttccgc ggcaagggcg aggtcggcat caccttctgc ggcgacggcg cctccaacca 1740 gggcactttc ctggaaagcc tgaacctggc cgcggtctgg aacctgccgg tgatcttcgt 1800 gatcgagaac aacggctatg ccgaatcgac ctcgcgcgat tacggcaccg cggtggacag 1860 ctacgtggac cgcgccgccg gcttcggcat ccccggcgtg acggtggacg gcaccgattt 1920 cttcgccgtc catgaagcgg ccggcgaagt gatccggcgc gcgcgcgaag gcggcgggcc 1980 gtcgctgctc gaatgcaaga tggtccgctt ctatggccac ttcgagggcg atgcgcagac 2040 ctaccgcgcc gccggcgaac tcgacgacat ccgcgccaac aaggattgcc tgaagctgtt 2100 tggccgcgcc gtgacccagg cgggagtggt cgctcgcgaa gaactcgaca ccatcgaccg 2160 cgaggtcgcc gcgctgatcg agcatgccgt gcaggaagcc aaggccgcgc cgcagccggg 2220 tcccgaagac ctgctcaccg acgtctacgt cagctactga tctgccacgc gctaacccaa 2280 tatcaggaga caaaccatgg ctcgcaaact gagcatcaag ctggcgatca acgaggcgat 2340 cgaccaggaa atgacccgcg accccagcgt catcatgctg ggcgaagata tcgtcggcgg 2400 cgccggcgcg gacggcgaga aggacgcctg gggcggcgtg ctgggcgtga ccaagggtct 2460 ttacgccaag cacggcgacc ggctgctgga cacgccgctg tctgaatccg cttacgtggg 2520 cgccgccatc ggcgccgcgg cctgcggcat gcgcccgatc gccgagctga tgtttatcga 2580 tttcatgggc gtttgcttcg accagatctt caaccaggcg gccaagttcc gctacatgtt 2640 cggcggcaag gcggagacgc cggtggtgat ccgcgcgatg gtcggcgcgg gctttcgcgc 2700 agccgcgcag cacagccaga tgctgacgcc gctgttcacg catatccccg gcctgaaggt 2760 ggtgtgcccg tccacgccgt acgacaccaa gggcctgctg atccaggcga tccgcgacaa 2820 cgacccggtg atcttctgcg agcacaagaa cctgtacggc ctggaaggcg aggtgcccga 2880 aggcgcctac gcgatcccgt tcggcgaggc caatatcgtg cgcgacggca aggacgtgtc 2940 gatcgtcacc tacgggttga tggtgcatcg cgcgctcgaa gcggcggcaa cgctggccaa 3000 ggagggcatc gaggccgaga tcgtggacct gcgcacgctc tcgccactgg acatggacac 3060 ggtgctggag tcggtcgaga acaccggccg cctggtggtg gtggacgagg ccagcccgcg 3120 ctgcaatatc gccaccgata tctccgcgca ggttgcgcag caagccttcg gcgcgctcaa 3180 ggccggcatc gagatggtgt gcccgccgca cacgccggtg ccgttctcgc cgacgctgga 3240 ggacctgtac atccccagcg ccgcgcagat cgcggccgcc gcgcgcaaga ccatgaaggg 3300 aggcaagcac tgatggcaac cgaaatttcc cccacgatta tcccgatcgt gatgcccaag 3360 tggggcctgt cgatgaagga aggcacggtc aatgcgtggc tggtcgacga aggcaccgag 3420 attaccgtgg gcctgccgat cctggatgtg gaaaccgaca agatcgccaa tgcggtggag 3480 gcgcccgacg ccggcacgct gcgccgcaag gtggcgcagg ccggcgacgt gctgccggtg 3540 aaggcgctgc tgggcgtgct ggcacctgcg gaggtgagcg atgcgcaaat cgatggctat 3600 gtcgcggctt atgaaacgcc ggcggacgac gctggcgaag aggacgccgc tgccgcgtac 3660 cagtttgccg acggcgacgg catccgggtc cgctatgccc gcaagggtgg cggcgccgaa 3720 accgtgctct tcatccacgg atttggcgga gacctggaca actggctgtt caacctcgat 3780 cccctggccg acgcgtacac cgtggtggcg ctcgacctgc ccggccacgg acagtcgtcg 3840 ccgcggctcg cgggcacgac gctggcgcag atggccggct ttgtagcgcg cttcatggac 3900 gagaccggga tcgaggcggc gcatgtggtc ggccattcga tgggcggcgg cgtggcggcg 3960 caactggccg tggatgcacc gcagcgggtg ctgtcggtgg cgctggtgtc gccggtgggc 4020 tttggcgacg ccgtcaacag cggctatacc gaaggcttcg tcagtgccca gtcccggcgc 4080 gagctcaagc ccgtggtcga actgctgttc gccgacgcgg ggctggtcag ccgccagatg 4140 ctggacgacc tgctgcgcta caagcggctg gacggcgtca ccgaggcgct gacggcgctg 4200 gggcagggac tcttcggcgg cggccgccag agcgagcagc ctggccagcg gctggccaac 4260 agcggcaagc gcgtgctggt ggtgtggggc ggtcaggacc agatcatccc ggccgcgcac 4320 gctgaagccg cgccgccggg agcaaccgtc aaggtctttg ccgatgctgg ccacatgagc 4380 cagatggaga aggccaatga cttcaacgcg ctgctgaaga agcatctggg cggctgatgc 4440 cgcgcgcgga tggcgccgcc cagggcgcca tccgctcggc cagctcgcct tgttccaatt 4500 caagacagga gacctcatga ccacagacct gaagacccgc ctgaccgaga tcaacaggca 4560 attcggtgcg ctcggccagg cccagcccgc cgccatgagc gccttccagg gcgtgatgaa 4620 ggccgccacg caggacggca gcctgcccgc cgccgtcaag gaactgattg ccgtcgcgct 4680 ggcggtgcag aagggctgcg acgactgcgt gctgttccac accagccagg cgctgcgcca 4740 tcgcgccacg cgcgagcagc tcgccgaagt gctggccatc aatatcgaga tgggtggcgg 4800 acccggcgcc atgtacgcgt cgaaggcgct ggcgtacttc gatgcgctca atgcctagtg 4860 ttgctagact cggcacgttg ctatccggag gttggtgcca tcccatgccg tttgaattta 4920 ttgatgcggc ccatgccggg caggcgggca gcgacccgct gcaggatgag ctggccttgc 4980 tcgacctcgc ggcacaaggc cggcaggtgg cgcagctgtg ggaggcgccg ctgtcgctgg 5040 tgggcgcgcg cacctacctg cgccatgccg cgctggaaac cgcgcgcgcc gacttcgcgc 5100 agcagggatg tccggtattc ctgcgcatgt ccggcggcgg gctggtgccg cagggccccg 5160 gcatcctcaa cctgagcctg gcctacacag tggaacagcc gccgggcgcg cgcagcgacg 5220 cggtctacct gcacttgtgc gaggtcatcg gcgacgcgct gcaatcgctg ggcgtcgac 5279 // |
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