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EBI DbfetchID M63594; SV 1; linear; genomic DNA; STD; PRO; 7385 BP. XX AC M63594; XX DT 12-OCT-1991 (Rel. 29, Created) DT 17-APR-2005 (Rel. 83, Last updated, Version 5) XX DE Photobacterium leiognathi lux operon (luxC, luxD, luxA, luxB, luxE, luxG) DE genes, complete cds. XX KW acyl transferase; acyl-CoA reductase; acyl-protein synthetase; KW luciferase alpha subunit; luciferase beta subunit; operon. XX OS Photobacterium leiognathi subsp. leiognathi OC Bacteria; Proteobacteria; Gammaproteobacteria; Vibrionales; Vibrionaceae; OC Photobacterium. XX RN [1] RP 1-7385 RX DOI; 10.1111/j.1432-1033.1991.tb16269.x RX PUBMED; 1915359. RA Lee C.-Y., Szittner R., Meighen E.A.; RT "The lux genes of the luminous bacterial symbiont, Photobacterium RT leiognathi, of the ponyfish. Nucleotide sequence, difference in gene RT organization, and high expression in mutant Escherichia coli"; RL Eur. J. Biochem. 201(1):161-167(1991). XX FH Key Location/Qualifiers FH FT source 1..7385 FT /organism="Photobacterium leiognathi subsp. leiognathi" FT /mol_type="genomic DNA" FT /tissue_lib="ATCC 25521" FT /db_xref="taxon:658" FT gene 745..2181 FT /gene="luxC" FT CDS 745..2181 FT /codon_start=1 FT /transl_table=11 FT /gene="luxC" FT /product="acyl-CoA reductase" FT /db_xref="GOA:P29236" FT /db_xref="InterPro:IPR008670" FT /db_xref="UniProtKB/Swiss-Prot:P29236" FT /protein_id="AAA25616.1" FT /translation="MIKKIPMIIGGVVQNTSGYGMRELTLNNNKVNIPIITQSDVEAIQ FT SLNIENKLTINQIVNFLYTVGQKWKSETYSRRLTYIRDLIKFLGYSQEMAKLEANWISM FT ILCSKSALYDIVENDLSSRHIIDEWIPQGECYVKALPKGKSVHLLAGNVPLSGVTSILR FT AILTKNECIIKTSSADPFTATALVNSFIDVDAEHPITRSISVMYWSHSEDLAIPKQIMS FT CADVVIAWGGDDAIKWATEHAPSHADILKFGPKKSISIVDNPTDIKAAAIGVAHDICFY FT DQQACFSTQDIYYIGDSIDIFFDELAQQLNKYKDILPKGERNFDEKAAFSLTERECLFA FT KYKVQKGESQSWLLTQSPAGSFGNQPLSRSAYIHQVNDISEVIPFVHKAVTQTVAIAPW FT ESSFKYRDILAEHGAERIIEAGMNNIFRVGGAHDGMRPLQRLVNYISHERPSTYTTKDV FT SVKIEQTRYLEEDKFLVFVP" FT gene 2195..3142 FT /gene="luxD" FT CDS 2195..3142 FT /codon_start=1 FT /transl_table=11 FT /gene="luxD" FT /product="acyl transferase" FT /db_xref="GOA:P21309" FT /db_xref="HSSP:1THT" FT /db_xref="InterPro:IPR003157" FT /db_xref="UniProtKB/Swiss-Prot:P21309" FT /protein_id="AAA25617.1" FT /translation="MENTQHSLPIDHVIDIGDNRYIRVWETKPKNKETKRNNTIVIASG FT FARRMDHFAGLAEYLANNGFRVIRYDSLNHVGLSSGEIKQFSMSVGKHSLLTVIDWLKE FT RNINNIGLIASSLSARIAYEVAAEIDLSFLITAVGVVNLRSTLEKALKYDYLQMEVNTI FT PEDLIFEGHNLGSKVFVTDCFENNWDSLDSTINKICELDIPFIAFTSDGDDWVCQHEVK FT HLVSNVKSDKKKIYSLVGSSHDLGENLVVLRNFYQSMTKAAVSLDRQLVELVDEIIEPN FT FEDLTVITVNERRLKNKIENEIINRLADRVLASV" FT gene 3180..4244 FT /gene="luxA" FT CDS 3180..4244 FT /codon_start=1 FT /transl_table=11 FT /gene="luxA" FT /product="luciferase alpha subunit" FT /db_xref="GOA:P29238" FT /db_xref="HSSP:1LUC" FT /db_xref="InterPro:IPR002103" FT /db_xref="UniProtKB/Swiss-Prot:P29238" FT /protein_id="AAA25618.1" FT /translation="MKISNICFSYQPPGESHQEVMERFIRLGVASEELNFDGFYTLEHH FT FTEFGITGNLYIACANILGRTKRIQVGTMGIVLPTEHPARHVESLLVLDQLSKGRFNYG FT TVRGLYHKDFRVFGTSQEDSRKTAENFYSMILDASKTGVLHTDGEVVEFPDVNVYPEAY FT SKKQPTCMTAESSETITYLAERGLPMVLSWIIPVSEKVSQMELYNEVAAEHGHDINNIE FT HILTFICSVNEDGEKADSVCRNFLENWYDSYKNATNIFNDSNQTRGYDYLKAQWREWVM FT KGLADPRRRLDYSNELNPVGTPERCIEIIQSNIDATGIKHITVGFEANGSEQEIRESME FT LFMEKVAPHLKDPQ" FT gene 4291..5271 FT /gene="luxB" FT CDS 4291..5271 FT /codon_start=1 FT /transl_table=11 FT /gene="luxB" FT /product="luciferase beta subunit" FT /db_xref="GOA:P29239" FT /db_xref="HSSP:1LUC" FT /db_xref="InterPro:IPR002103" FT /db_xref="UniProtKB/Swiss-Prot:P29239" FT /protein_id="AAA25619.1" FT /translation="MNFGLFFLNFQPEGMTSEMVLDNMVDTVALVDKDDYHFKRVLVSE FT HHFSKNGIIGEPLTAISFLLGLTKRIEIGSLNQVITTHHPVRIGEQTGLLDQMSYGRFV FT LGLSDCVNDFEMDFFKRKRSSQQQQFEACYEILNEALTTNYCQADDDFFNFPRISVNPH FT CISEVKQYILASSMGVVEWAARKGLPLTYRWSDSLAEKEKYYQRYLAVAKENNIDVSNI FT DHQFPLLVNINENRRIARDEVREYIQSYVSEAYPTDPNIELRVEELIEQHAVGKVDEYY FT DSTMHAVKVTGSKNLLLSFESMKNKDDVTKLINMFNQKIKDNLIK" FT gene 5436..6557 FT /gene="luxE" FT CDS 5436..6557 FT /codon_start=1 FT /transl_table=11 FT /gene="luxE" FT /product="acyl-protein synthetase" FT /db_xref="GOA:P29334" FT /db_xref="InterPro:IPR007534" FT /db_xref="UniProtKB/Swiss-Prot:P29334" FT /protein_id="AAA25620.1" FT /translation="MSTLLNIDATEIKVSTEIDDIIFTSSPLTLLFEDQEKIQKELILE FT SFHYHYNHNKDYKYYCNIQGVDENIQSIDDIPVFPTSMFKYSRLHTADESNIENWFTSS FT GTKGVKSHIARDRQSIERLLGSVNYGMKYLGEFHEHQLELVNMGPDRFSASNVWFKYVM FT SLVQLLYPTTFTVENDEIDFEQTILALKAIQRKGKGICLIGPPYFIYLLCHYMKEHNIE FT FNAGAHMFIITGGGWKTKQKEALNRQDFNQLLMETFSLFHESQIRDIFNQVELNTCFFE FT DSLQRKHVPPWVYARALDPVTLTPVEDGQEGLMSYMDASSTSYPTFIVTDDIGIVRHLK FT EPDPFQGTTVEIVRRLNTREQKGCSLSMATSLK" FT gene 6573..7277 FT /gene="luxG" FT CDS 6573..7277 FT /codon_start=1 FT /transl_table=11 FT /gene="luxG" FT /db_xref="GOA:P29237" FT /db_xref="HSSP:1QFJ" FT /db_xref="InterPro:IPR001221" FT /db_xref="UniProtKB/Swiss-Prot:P29237" FT /protein_id="AAA25621.1" FT /translation="MIFNCKVKKVEASDSHIYKVFIKPDKCFDFKAGQYVIVYLNGKNL FT PFSIANCPTCNELLELHVGGSVKESAIEAISHFINAFIYQKEFTIDAPHGDAWLRDESQ FT SPLLLIAGGTGLSYINSILSCCISKQLSQPIYLYWGVNNCNLLYADQQLKTLAAQYRNI FT NYIPVVENLNTDWQGKIGNVIDAVIEDFSDLSDFDIYVCGPFGMSRTAKDILISQKKAN FT IGKMYSDAFSYT" XX SQ Sequence 7385 BP; 2554 A; 1276 C; 1348 G; 2207 T; 0 other; tctagacttt gtatattttc aatttaatac caaacgataa atctaagagg attttagttt 60 tttaaagata aaatacaaaa tacggacaaa ttttttttat tatcatcaca aatattttaa 120 tttaaaaaca taaagaacaa tcttgtattt attctaaaac accaataata ttctggtggg 180 agatagtaat atcattaatt acataccata tatagacaaa cactacctag cgataataaa 240 tcccatcata cccacaccaa aaaaaaataa aaaccaccca atataacaaa gacataacta 300 caaccagaaa gaattaaaat aaatttcacc attcatttaa gttatgttta ttccatcaat 360 aataaaaact ataaaaatac cattcaacct aatttaaaaa ttccggagta aattaaaaat 420 aaagataaaa agtaaagcca ataaataaaa tcacaaaaaa cataaaaaac gaattaataa 480 taaaaaattt tgtaatatat ttcacttata ccatcatcta tcatgaaaaa tgaaaaataa 540 aaaaatcaga aagattaaat ataaaaatat taaattaaaa actgatttta ttaatagtgt 600 gaccaaaatc tcgaatagaa aaaagaaaaa ataaattagt tatcacatcc atatttcttt 660 atctaatctg attcagctag ctcatgcggc atagtttata ccgaaactac atactcagca 720 tgtgcgaata ccaaaggaga ttacatgatt aagaagatcc caatgattat tgggggtgta 780 gttcaaaaca cgtctggata tggcatgcgt gaactaacgc tcaacaataa taaagtgaat 840 atccctatca tcacccaaag tgatgttgaa gctattcaat cactaaatat agaaaacaaa 900 ttgactataa atcagatagt taatttctta tatacagtgg gacaaaaatg gaagagcgaa 960 acttacagcc gacgactcac ttatattcga gatcttatta agttcctcgg ttactcacaa 1020 gagatggcaa aacttgaagc taactggatc tcaatgattc tgtgtagcaa aagtgcgttg 1080 tacgatattg ttgagaatga tcttagctca cggcatatta ttgatgagtg gatcccccaa 1140 ggtgaatgtt atgtcaaagc gctcccaaaa ggaaaatctg tacacctatt agctggtaac 1200 gtaccactat ctggtgtgac ttctattctt cgtgcgattt tgaccaaaaa cgagtgcatc 1260 ataaaaacgt catcagctga tccttttaca gctactgcgc tagttaatag ttttatcgat 1320 gtagatgcag aacacccgat cacacgttca atctcagtta tgtattggtc acatagcgag 1380 gatcttgcta ttccaaaaca aataatgagc tgtgctgatg tggttattgc atggggtggt 1440 gatgatgcaa ttaaatgggc tacagaacat gcaccatcac acgcagatat tctaaaattt 1500 ggtcccaaaa agagtatatc cattgttgac aacccaacag atattaaggc tgctgctatc 1560 ggtgtagcac atgatatctg tttttacgat cagcaagcat gtttctccac ccaagatatt 1620 tattatattg gcgatagcat agacatattt tttgatgaat tagctcagca attaaataaa 1680 tataaagaca tattgcctaa aggtgagcgg aattttgatg aaaaagcagc tttttcttta 1740 acggaaagag aatgtttgtt tgccaaatat aaagttcaaa aaggtgaaag ccaatcttgg 1800 ttattaacgc aatcacctgc gggatcattt ggtaatcagc cgttatcacg ctcggcttat 1860 attcatcaag taaatgacat ttcagaagtc attccattcg tgcataaggc ggtaacgcaa 1920 accgtcgcaa tagcgccgtg ggagtcgtct ttcaaatata gagatatatt agcagaacat 1980 ggtgcagaac gaattataga agccggaatg aataatatat ttcgagtagg tggcgcccat 2040 gatgggatgc gtccccttca acggcttgtt aactatatat cacatgaaag gccgtcaaca 2100 tataccacta aagatgtctc ggtgaaaatc gaacagactc gttatcttga ggaagataag 2160 ttcctcgtat ttgtaccgta gaaagagata tatcatggaa aatacacaac attcattacc 2220 tattgatcac gtaattgata ttggtgataa ccgttatatt cgagtatggg aaaccaagcc 2280 gaaaaataaa gaaaccaagc gtaataatac catcgttatc gcctcaggct ttgctcgacg 2340 catggatcat tttgctggtc ttgccgaata tttagcaaat aatggttttc gtgttattcg 2400 ttatgattcg ttaaatcatg tcggtcttag tagcggagag atcaaacagt tctcgatgtc 2460 agtaggtaaa cacagtttgc taactgttat tgattggcta aaagaacgaa atattaacaa 2520 tataggtctt attgcttcga gtctttctgc tcgaattgct tatgaagtgg cagcagaaat 2580 tgatttgtca tttttaatta ccgccgtcgg tgttgtcaat ttaagaagta cgctagaaaa 2640 agcactgaaa tatgattatc tacaaatgga agtaaatact attcctgaag atttaatttt 2700 tgaaggacac aatctaggtt caaaagtctt tgtgacagat tgttttgaaa ataattggga 2760 ctcattagat tcgacaataa ataaaatttg tgaactagat attccattta ttgctttcac 2820 ttcagatggc gatgattggg tttgccaaca tgaagtaaaa catttagtca gtaacgttaa 2880 atctgacaaa aagaaaattt actcactcgt tggctcatct catgatttgg gcgaaaacct 2940 agtggtgctt cgtaacttct atcaatcaat gacgaaagct gctgtgagct tagatcgtca 3000 attagtagag cttgttgatg aaattattga accaaatttt gaagacctaa cagttattac 3060 ggtaaatgaa cggcgcctca aaaataaaat cgaaaatgaa attattaata gattagctga 3120 tcgcgtattg gctagtgtct aaatagtact tacctaagta cagccaaaag gaagaaataa 3180 tgaaaattag taatatctgt ttctcatacc aaccaccagg tgaatcacat caagaggtaa 3240 tggagcgctt tattcgttta ggcgttgcat cagaagagct caactttgat ggtttctata 3300 cacttgaaca ccatttcact gagtttggta ttacaggtaa cctttatatt gcctgtgcca 3360 atattcttgg tcgaaccaaa aggatccaag tcggtaccat ggggatagtg ttaccgacag 3420 agcacccagc acgacatgta gaaagtcttc tcgttttaga tcaactgtct aaagggcgct 3480 ttaactacgg tactgttcgc ggactctacc ataaagattt tcgtgttttt ggtacatcac 3540 aggaagattc tcgtaagacc gcagaaaatt tctactctat gatcttggat gcatcaaaaa 3600 caggtgtgct acatactgac ggtgaagtag tagagttccc agatgtcaat gtttatccag 3660 aagcttacag caaaaaacaa cccacctgca tgacagccga atcatccgag accatcactt 3720 atttagctga acgtggttta ccaatggtgt taagttggat tattccggtc agtgagaaag 3780 tctcacaaat ggaattgtac aatgaagttg cggcagagca tggtcatgac attaacaaca 3840 ttgaacatat cctaactttc atttgctctg taaatgaaga cggtgaaaaa gcagacagcg 3900 tatgccgtaa tttcctagaa aattggtacg actcttacaa aaatgcaacc aacatcttca 3960 acgacagtaa ccaaactcgt ggctacgatt acctcaaagc tcagtggcgt gagtgggtaa 4020 tgaaggggtt ggctgatcct cgtcgccgac ttgattacag taacgaatta aaccctgtcg 4080 gcacgccaga acgatgcatt gagatcattc aaagtaatat tgatgccact ggaattaagc 4140 atattactgt tggatttgaa gcgaatggtt ctgaacaaga aattcgtgaa tccatggagc 4200 tatttatgga aaaagtagcg ccacacttaa aagatcctca gtaagctgtt ctttttaaac 4260 tattcaatat caaggcataa ggaataaaat atgaatttcg ggttattttt cctaaatttc 4320 cagcctgaag gtatgacttc agaaatggtt ttagacaaca tggtagatac tgtcgcatta 4380 gtggataaag atgattacca ctttaaaaga gtgctcgtca gcgagcatca tttttctaaa 4440 aacggcatta tcggagaacc tttgacagcg attagcttct tacttggttt gactaaacgt 4500 atagaaattg gttctttaaa tcaagtgatt accacccatc atcctgtacg tatcggagaa 4560 caaacgggct tacttgatca aatgtcttac ggtcgtttcg ttttaggctt aagtgactgt 4620 gtcaatgact tcgaaatgga tttctttaag agaaaacgta gctctcaaca gcaacaattc 4680 gaagcatgtt acgaaatttt aaatgaagcg ctgacgacaa actattgtca ggcagatgat 4740 gacttcttta acttcccacg tatttctgtt aacccgcatt gtattagcga agtaaaacaa 4800 tatattttag cttcaagcat gggcgtggtt gaatgggcag caagaaaagg attgccactc 4860 acttaccgct ggagtgacag cctagcagaa aaagaaaaat actatcagcg ttatctcgct 4920 gttgctaaag agaataatat tgatgtatca aatattgacc accaattccc actgctcgtt 4980 aatatcaatg aaaatcgtcg tattgctcga gatgaagtaa gggagtatat acaaagttat 5040 gtgagtgaag cctaccctac tgaccccaac attgagctaa gagtagaaga gcttattgag 5100 cagcatgctg tcggcaaagt ggatgagtac tacgactcaa caatgcacgc agtaaaagtt 5160 acaggttcaa aaaatttatt actctctttt gaatcaatga aaaataaaga cgatgttacc 5220 aagcttataa atatgtttaa tcaaaaaatc aaagataacc ttattaaata atttaattac 5280 ggatagatat tttcgatata tctaagtctt actaccattt atataaacta tttatacaga 5340 taacgtttca tttgattaag tcagtaaata attgccatta attaatggca gtgcagatcc 5400 ttacactgcc atttataaat taaataaggg ttaacatgtc aacattatta aatatagatg 5460 caactgaaat taaggtgagt acagaaatag atgatattat ttttacatca tcaccgctaa 5520 cgttactatt tgaagatcaa gaaaaaatac agaaagaact tattttggag tctttccatt 5580 atcattacaa tcataataaa gattataagt actattgtaa tatacaaggc gtagatgaga 5640 atatacagtc cattgacgat attcctgttt ttcctacttc aatgttcaag tactcaagat 5700 tacatactgc tgatgaatca aatattgaaa attggtttac tagtagtggt acaaagggag 5760 tcaaaagtca tatagctcga gatcggcaga gtattgaacg cttgctaggt tctgttaatt 5820 acggcatgaa atacttgggt gaatttcacg agcatcaatt agaactagtg aatatggggc 5880 cagatcgttt cagtgcgtca aatgtttggt ttaaatatgt aatgagctta gttcaattac 5940 tttacccaac aacatttacc gttgaaaacg atgaaatcga ttttgaacaa accatcttag 6000 cgttaaaagc aattcagcgt aaaggaaaag gaatttgttt aattggccct ccgtatttta 6060 tttatttgtt atgccactac atgaaagagc ataatatcga atttaatgct ggtgcacata 6120 tgtttatcat tacaggtggg ggatggaaaa ccaaacaaaa agaagcgcta aaccgacaag 6180 atttcaatca actattgatg gagactttta gccttttcca tgaaagtcaa attcgagata 6240 tctttaacca agtagagcta aacacttgtt tctttgaaga cagcctacag cgtaaacatg 6300 taccaccgtg ggtatatgct cgtgcgcttg atcctgtcac tttaacgccc gtagaagatg 6360 gccaagaggg cttgatgagt tatatggatg cctcatctac cagctacccg acatttattg 6420 ttaccgacga tattggtatt gttcgccatc taaaagaacc agatccattc caaggaacaa 6480 cggttgaaat tgttcgtcgt ttaaatacgc gagaacaaaa aggatgttca ctctcaatgg 6540 ccacgagcct gaaataaaag cagggcttaa tcatgatttt taattgcaag gttaaaaaag 6600 tcgaagcatc tgacagccat atttacaaag tgtttattaa gcctgacaaa tgctttgatt 6660 ttaaagcggg tcaatatgta attgtgtatc tcaatggaaa aaatttgccg ttttctattg 6720 ctaactgccc aacttgtaat gagctccttg aattacatgt aggaggttcg gtaaaagaat 6780 ccgccattga agctatttcg cactttatta atgcatttat ttatcaaaaa gaatttacaa 6840 tcgatgcacc acacggtgat gcatggctga gagatgaaag ccaatcacct ttactactta 6900 tagcaggagg gacaggttta tcatatatca atagcatttt aagttgttgt attagtaaac 6960 agttatctca gcctatctat ctttattggg gagtaaataa ctgtaattta ctctatgctg 7020 atcaacaact aaaaacactc gccgcacaat acagaaatat aaattatatt cctgtggtag 7080 agaatttaaa tactgactgg cagggaaaaa ttggtaatgt tattgacgcg gttattgaag 7140 atttttcaga tttatctgac tttgatatct atgtctgcgg gccatttggt atgagccgga 7200 ctgcgaaaga tattctgatc tcacagaaaa aggcgaatat aggaaaaatg tattctgatg 7260 catttagcta tacgtaatta aaatcattat ttaactctaa ataaaaccgt tattaatttt 7320 tcgacctact tattctgggt actgataatt agtacccaat agatagttct atttataggg 7380 atatt 7385 // |
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