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EBI DbfetchID M61151; SV 1; linear; genomic DNA; STD; PRO; 5995 BP. XX AC M61151; J03688; XX DT 27-AUG-1993 (Rel. 36, Created) DT 18-APR-2005 (Rel. 83, Last updated, Version 4) XX DE Agrobacterium rhizogenes trytophan oxygenase (aux1) and hydrolase (aux2) DE genes, complete cds. XX KW aux1 gene; aux2 gene; hydrolase; tryptophan oxygenase. XX OS Agrobacterium rhizogenes OC Bacteria; Proteobacteria; Alphaproteobacteria; Rhizobiales; Rhizobiaceae; OC Rhizobium/Agrobacterium group; Agrobacterium. XX RN [1] RP 1-5995 RX PUBMED; 1932811. RA Camilleri C., Jouanin L.; RT "The TR-DNA region carrying the auxin synthesis genes of the Agrobacterium RT rhizogenes agropine-type plasmid pRiA4: nucleotide sequence analysis and RT introduction into tobacco plants"; RL Mol. Plant Microbe Interact. 4(2):155-162(1991). XX FH Key Location/Qualifiers FH FT source 1..5995 FT /organism="Agrobacterium rhizogenes" FT /strain="A4" FT /mol_type="genomic DNA" FT /db_xref="taxon:359" FT misc_signal 1021..1045 FT /function="left 24 bp signal sequence for T-DNA transfer" FT /experiment="experimental evidence, no additional details FT recorded" FT gene complement(1436..2836) FT /gene="aux2" FT CDS complement(1436..2836) FT /codon_start=1 FT /transl_table=11 FT /gene="aux2" FT /product="hydrolase" FT /function="second step in auxin synthesis; pathway encoded FT by TR-DNA in plant cells transformed by Agrobacterium FT rhizogenes strain A4. Conversion of indole-3-acetamide into FT auxin" FT /db_xref="GOA:Q09102" FT /db_xref="HSSP:1M21" FT /db_xref="InterPro:IPR000120" FT /db_xref="UniProtKB/Swiss-Prot:Q09102" FT /protein_id="AAA22079.1" FT /translation="MVTLSSITETLKCLRERKYSCFELIETIIARCEAARSLNAFLETD FT WAHLRWTASKIDQHGGAGVGLAGVPLCFKANIATGRFAATAGTPGLQNHKPKTPAGVAR FT QLLAAGALPGASGNMHELSFGITSNNFATGAVRNPWNPSLIPGGSSGGVAAAVAGRLML FT GGVGTDTGASVRLPAALCGVVGFRPTVGRYPTDGIVPVSPTRDTPGVIAQNVPDVILLD FT GIICGRPPVNQTVRLKGLRIGLPTAYFYNDLEPDVALAAETIIRVLARKDVTFVEADIP FT DLAHHNEGVSFPTAIYEFPLSLEHYIQNFVEGVSFSEVVRAIRSPDVASILNAQLSDNL FT ISKSEYCLARRFFRPRLQAAYHSYFKAHQLDAILFPTAPLTAKPIGHDLSVIHNGSMTD FT TFKIFVRNVDPSSNAGLPGLSLPVSLSSNGLPIGMEIDGSASSDERLLAIGLAIEEAID FT FRHRPTLS" FT gene 3230..5479 FT /gene="aux1" FT CDS 3230..5479 FT /codon_start=1 FT /transl_table=11 FT /gene="aux1" FT /product="tryptophan oxygenase" FT /function="first step of auxin synthesis pathway encoded by FT the TR-DNA in plant cells transformed by Agrobaterium FT rhizogenes strain A4. Conversion of tryptophan into FT indole-3-acetamide" FT /db_xref="GOA:Q09109" FT /db_xref="InterPro:IPR012142" FT /db_xref="UniProtKB/Swiss-Prot:Q09109" FT /protein_id="AAA22080.1" FT /translation="MAGSSFTLPSTGSAPLDMMLIDDSDLLQLGLQQVFSKRYTETPQS FT RYKLTRRASPDVSSGEGNVHALAFIYVNAETLQMIKNARSLTEANGVKDLVAIDVPPFR FT NDFSRALLLQVINLLGNNRNADDDLSHFIAVALPNSARSKILTTAPFEGSLSENFRGFP FT ITREGNVACEVLAYGNNLMPKACSDSFPTVDLLYDYGKFFESCAADGRIGYFPEGVTKP FT KVAIIGAGFSGLVAASELLHAGVDDVTVYEASDRLGGKLWSHGFKSAPNVIAEMGAMRF FT PRSESCLFFYLKKHGLDSVGLFPNPGSVDTALFYRGRQYIWKAGEEPPELFRRVHHGWR FT AFLQDGYLHDGVMLASPLAIVDALNLGHLQQAHGFWQSWLTYFERESFSSGIEKMFLGN FT HPPGGEQWNSLDDLDLFKALGIGSGGFGPVFESGFIEILRLVVNGYEDNVRLSYEGISE FT LPHRIASQVINGRSIRERTIHVQVEQIDREEDKINIKIKGGKVEVYDRVLVTSGFANIE FT MRHLLTSSNAFFHADVSHAIGNSHMTGASKLFLLTNEKFWLQHHLPSCILTTGVAKAVY FT CLDYDPRDPSGKGLVLISYTWEDDSHKLLAVPDKRERFASLQRDIGRAFPDFAKHLTPA FT DGNYDDNIVQHDWLTDPHAGGAFKLNRRGNDVYSERLFFQPFDVMHPADDKGLYLAGCS FT CSFTGGWVHGAIQTACNATCAIIYGSGHLQELIHWRHLKEGNPLAHAWKRYRYQA" XX SQ Sequence 5995 BP; 1552 A; 1429 C; 1482 G; 1532 T; 0 other; gtcgacagtc gcaacagcaa tcgaggggtg ttgatcaacc ttggccagtt gcccttcgtc 60 cctatcagta acgacaacct tgtaatcgcc ggtttccgca agcatcagag caatggctcc 120 gccaatctta cccgcgccga taaccactat ttctttcata caaatcccct gtgttctgta 180 tataggatat ttataaaaat aaccggggat tggtcgaatc atagcggcaa ggtgggcatt 240 atgttatata tttttgtgca aaaagacgac tgcacttggt aatatgaagg ggttccgcat 300 gcaattgacc gaaagggatc gggagctcct gtcgttactc ggcgaaaatg ccagaacgcc 360 tgtggcgacg ctcgcgaaaa agctttctct atccaggacc acagtgcagg ctcgcttgga 420 gcgccttgag agagagggag ttattgtcgg ctatggtgta aggctatcga atgaatattc 480 ctcgagcttg gttcgagccc atattctgat caccattacg aaggcgcttt cacaggtgac 540 ggcctccctt gggaaggtca cggccgttat cgctcttcat tctgtgagtg gcacttttga 600 tttgattgcg attatcgaag ccccttcgat ttcagagctc gatcagttca tcgatcaaat 660 cggcataatt gaaggtgtcg agcggacgct ctcttcgatt attctttcaa cacgcatttc 720 acgctgaaag agcaagaggc cacagccgtg cgcctgctgc cacctttccg acgatgacca 780 tgcagaactt acggcctcta tgtcggtgta ttccacccac cgacgcgcca tttccgtgtg 840 cattaagata atgctttccg ccccatcgga ttgctgttct gacatggtat atcattacga 900 cagccaatgt ttggcggctc gtacgaatca tcaagcggtc aacctgtgct gtcaaccgtc 960 acgatatgag caagcaactc aaacgctttc ctattcaccg cacgcgcgtt gttggctgtt 1020 tggcaggata tatgccaacg taaaaatgag ggcaatcgat tgtactgaat cggattttca 1080 agggtctggc caaaactatt ccgtgggcac ctggcacacg ccctggagtc cggcccgttt 1140 ccagttgagg gttgtctacg cttagatgag aaggaaagtt gtccaagacg aatcccagtg 1200 tcctattacc aatagccggc gctagtttga tttcagaata aagagaaatt cgtcacacca 1260 aatattagaa gcaatgtttg attgaccatc atacttaaga tgaacattcc aaacacagtt 1320 ataatacgca ttattattga cacaatataa aattataatg ttgatattcc ttgaaatata 1380 atattacaat agataaagta gaggaaatta tgtcagaaat ttggtttggc tctgcttacg 1440 acagagtcgg acgatgccta aagtctattg cttcttctat cgctagtcca attgctaaca 1500 gacgttcatc cgagcttgca gagccatcga tttccatgcc aataggcaga ccgttggaac 1560 taagagaaac gggaagactt aggcccggca ggcccgcatt actgctggga tctacattcc 1620 gcacgaagat tttaaaggta tcggtcattg agccattgtg aatcaccgat agatcatggc 1680 caattggctt ggctgtcaac ggagctgttg ggaaaagaat tgcatctagc tgatgcgcct 1740 tgaagtaact gtggtaggcc gcttggagtc tcggtctgaa aaaacgtcgc gccagacaat 1800 actcgctttt ggaaataaga ttatccgaga gttgtgcatt gagaatactt gcaacatccg 1860 gactgcgaat cgctctgaca acctcagaaa aggaaacacc ctctacgaag ttctgaatat 1920 aatgttcaag ggacaacgga aattcgtaga tggcagtcgg aaagctgacc ccttcattgt 1980 gatgcgctaa atcaggaata tctgcttcaa caaaagtaac atctttgcgt gccagaactc 2040 tgataatcgt ctcggctgct aaggcgacat cgggctccag gtcgttgtaa aagtaagcgg 2100 ttggcaagcc tatacgcagc cccttcaggc ggaccgtttg attaaccggc ggtctcccgc 2160 aaatgatacc gtcaagaaga atcacgtccg gaacattctg tgcgataacg ccaggggtgt 2220 cccgggtggg gcttaccgga actattccgt ccgttggata tcgccccacg gtaggacgaa 2280 accccaccac gccgcacaag gcggccggta aacggaccga cgctcccgtg tcagttccga 2340 cgccgcccag catcaatcgg ccggccaccg cggcggccac acccccactt gatccccctg 2400 ggatgagact agggttccac gggtttcgta cggcgcctgt ggcgaagttg ttgctcgtga 2460 tcccaaaaga caattcgtgc atgtttcccg aagcgccagg cagtgcccca gccgcgagaa 2520 gttgtcgtgc aactccggca ggcgtcttgg gtttgtggtt ctgtaagcct ggcgtaccag 2580 cggtcgcggc gaacctgcct gtcgcaatat tcgctttaaa gcatagggga acgccagcta 2640 ggccaacacc ggcacctccg tgttgatcga ttttgctggc agtccaccgt aggtgcgccc 2700 agtcggtttc cagaaaggcg tttaaggatc ttgctgcttc acagcgggct attatcgttt 2760 cgattaactc aaagcacgag tattttcttt ccctgagaca tttaagcgtc tcggtgatcg 2820 aggagagggt caccattttc gttgtgctga gggaactgag atagatctcg ccagagaaac 2880 gttcaatgat ttttgcttgg agtgaaaaag gcaaataatt atagaggaag gaagtcagaa 2940 atgctgcgca gtagggccac ttgtataagt gccggtcgaa cactgctggt ggaaagtcaa 3000 aagcgtgaag tattagttga actctgttac taaattgaga taaatgggat attttattcg 3060 aaagtactgt ttgagatcta gcgacaataa taatgtcatc ttatgagatt gcatggcaat 3120 atggatctaa tatttggcat aaatagatgg tggttttgtc tccactttta aaccttcaca 3180 gcgttaccct aacacctctt aattgcgtac actcctttca accgcatcaa tggctggatc 3240 ctccttcaca ttgccatcaa ctggctcagc gccccttgat atgatgctta tcgatgattc 3300 agatctgctg caattgggtc tccagcaggt attctcgaag cggtacacag agacaccgca 3360 gtcacgctac aaactgacca ggagggcttc tccagacgtc tcatctggcg aaggcaatgt 3420 gcatgccctt gcgttcatat atgtcaacgc tgagacgttg cagatgatca aaaacgctcg 3480 atcgctaacc gaagcgaacg gcgtcaaaga tcttgtcgcc atcgacgttc cgccatttcg 3540 aaacgacttc tcaagagcgc tactccttca agtgatcaac ttgttgggaa acaaccgaaa 3600 tgccgatgac gatcttagtc acttcatagc agttgctctc ccaaacagcg cccgctctaa 3660 gatcctaacc acggcaccgt tcgaaggaag cttgtcagaa aacttcaggg ggttcccgat 3720 cactcgtgaa ggaaatgtgg catgtgaagt gctagcctat gggaataact tgatgcccaa 3780 ggcctgctcc gattcctttc caaccgtgga tcttctttat gactatggca agttcttcga 3840 gagttgcgcg gccgatggac gtatcggtta ttttcctgaa ggcgttacga aacctaaagt 3900 ggctataatt ggcgcaggct tttccgggct cgttgcagcg agcgaactac ttcatgcagg 3960 ggtagacgat gttacggtgt atgaggcgag tgatcggctt ggaggaaagc tatggtcaca 4020 cggatttaag agtgctccaa atgtgatagc cgagatgggg gccatgcgtt ttccgcgaag 4080 tgaatcatgc ttgttcttct atctcaaaaa gcacggactg gactccgttg gtctgttccc 4140 gaatccggga agtgtcgata ccgcattgtt ctacaggggc cgtcaatata tctggaaagc 4200 gggagaggag ccaccggagc tgtttcgtcg tgtgcaccat ggatggcgcg catttttgca 4260 agatggctat ctccatgatg gagtcatgtt ggcgtcaccg ttagcaattg ttgacgcctt 4320 gaatttaggg catctacagc aggcgcatgg cttctggcaa tcttggctca catattttga 4380 gcgagagtct ttctcttctg gcatcgaaaa aatgttcttg ggcaatcatc ctccgggggg 4440 tgaacaatgg aattccctag atgacttgga tcttttcaaa gcgctgggta ttggatccgg 4500 cggattcggc cctgtatttg aaagtgggtt tatcgagatc cttcgcttag tcgtcaacgg 4560 gtatgaggat aacgtgcggc tgagttacga aggaatttct gagctgcctc ataggatcgc 4620 ctcacaggta attaacggca gatctattcg cgagcgtaca attcacgttc aagtcgagca 4680 gattgataga gaggaggata aaataaatat caagatcaaa ggaggaaagg ttgaggtcta 4740 tgatcgagta ctggttacat ccgggtttgc gaacatcgaa atgcgccatc tcctgacatc 4800 aagcaacgca ttcttccatg cagatgtaag ccatgcaata gggaacagtc atatgactgg 4860 tgcgtcaaaa ctgttcttgc tgactaacga aaaattctgg ctacaacatc atttgccatc 4920 gtgcatactc accaccggcg ttgcaaaggc agtttattgc ttagactatg atccgcgaga 4980 tccaagcggc aaaggactgg tgttgataag ctatacttgg gaggatgact cacataagct 5040 cctagccgtc cccgacaaaa gagaaaggtt cgcatcgctg cagcgcgata ttgggagggc 5100 attcccagat tttgccaagc acctaactcc tgcagacggg aactatgatg ataatatcgt 5160 tcaacatgat tggctgactg atccccacgc tggcggagcg tttaaactga accgcagagg 5220 caacgacgta tattcagaaa ggcttttctt tcagcccttt gacgtaatgc atcccgcgga 5280 cgataaggga ctttacttgg ccggttgtag ctgttccttc accggagggt gggttcatgg 5340 tgccattcag accgcatgca acgctacgtg tgcgatcatt tatggttccg gacacctgca 5400 agagctaatc cactggcgac acctcaaaga aggtaatcca ctggcgcacg cttggaagcg 5460 gtataggtat caagcgtgat aatgcaacag ttagaataat tagtttgccc tagccggtat 5520 tccttggtgt tccaataggg ttccgaagcc aataggcgaa aaagctgact tttcagtccc 5580 ttttattatt caattcgctt cggtccaagc ataattgtaa cgctacgtga tagaaaaatg 5640 gaaattgaca gattacttac ttaaattaat ataatctatt aatatcgtca agctaaaaac 5700 atgtaatacg taaatatatg gaaactttat gtctgaaaag accacattat tattgatcgt 5760 aatacactga actggtcata acagggaagg ctaactgcaa catatcctat aaatactcag 5820 tgaaaatggc cgctccccaa tgttaagcca tttttgcggt cgggctaagc gctcgtccgt 5880 gtctcccctg gcccgagtgt cggctctcca tcagcggcct catcatctgt cgctgacacc 5940 ggtggcccca atttcaaatc gaggaaagac gatgccctcg ccggcaaacg tcgac 5995 // |
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