EBI Dbfetch

ID   M60851; SV 1; linear; genomic DNA; STD; PRO; 2100 BP.
XX
AC   M60851;
XX
DT   13-MAR-1991 (Rel. 27, Created)
DT   14-NOV-2006 (Rel. 89, Last updated, Version 5)
XX
DE   L.casei phosphotransferase system lactose-specific enzyme II (lacE) gene,
DE   complete cds.
XX
KW   lactose-specific enzyme II;
KW   phosphoenolpyruvate-dependent phosphotransferase system.
XX
OS   Lactobacillus casei
OC   Bacteria; Firmicutes; Lactobacillales; Lactobacillaceae; Lactobacillus.
XX
RN   [1]
RP   1-2100
RX   PUBMED; 2125053.
RA   Alpert C.-A., Chassy B.M.;
RT   "Molecular cloning and DNA sequence of lacE, the gene encoding the
RT   lactose-specific enzyme II of the phosphotransferase system of
RT   Lactobacillus casei. Evidence that a cysteine residue is essential for
RT   sugar phosphorylation";
RL   J. Biol. Chem. 265(36):22561-22568(1990).
XX
FH   Key             Location/Qualifiers
FH
FT   source          1..2100
FT                   /organism="Lactobacillus casei"
FT                   /mol_type="genomic DNA"
FT                   /db_xref="taxon:1582"
FT   CDS             <1..228
FT                   /codon_start=1
FT                   /transl_table=11
FT                   /label=URF
FT                   /db_xref="GOA:P24401"
FT                   /db_xref="HSSP:1L1C"
FT                   /db_xref="InterPro:IPR001550"
FT                   /db_xref="UniProtKB/Swiss-Prot:P24401"
FT                   /protein_id="AAA72983.1"
FT                   /translation="LQRVDTYAVTIVPEVATELKQNYPQSYKIASEIFDEIKDQLYRSM
FT                   SEDERLYFIIHIQRLINEAPAQNHSQNDSL"
FT   gene            349..2082
FT                   /gene="lacE"
FT   CDS             349..2082
FT                   /codon_start=1
FT                   /transl_table=11
FT                   /gene="lacE"
FT                   /product="phosphotransferase system lactose-specific enzyme
FT                   II"
FT                   /db_xref="GOA:P24400"
FT                   /db_xref="InterPro:IPR004801"
FT                   /db_xref="UniProtKB/Swiss-Prot:P24400"
FT                   /protein_id="AAA72984.1"
FT                   /translation="MNKVFDKLKPVFEAIAANKYISAIRDGFIACMPIIIFSSIFMMVA
FT                   YVPNAWGFYWPDNVTNTLMVAYNYSMGLLALFVAGTTAKNLTDSKNLELPKTNQINPVA
FT                   VIVASEISFVILSILPLKTGVDLTYMGTQGLICAYIVGLIVPNIYYVCIKNNVTIKLPE
FT                   QVPGNIAQSFKDLIPMGLSVTAFWLFGVGFKAATGTVLPRWIIQVLSPLFQASDSYLGL
FT                   ALIAGAMAFFWFCGVQGPSIVQPAVVPIMIANTAANLQQYQAGQHVSHVLAMNTMDYVM
FT                   NFGGTGATLVVPFIMLFAARSAQLKAVGKAAFVPCTFGVNEPVLFGMPIIMNPMLFIPF
FT                   LATPIVNVCLFKFFVSVLGMNSMMYTMPWTVPGPIGILISTGFAPLAFVFVLLTLVLDV
FT                   AIYFPFIRVYDSTLLAEEKAKEEVIEDDGMAVLASDTVSPSIPTGLTVATATDDDATHV
FT                   LPETAPSAHGEAYFKQNEVDVLVLCAGGGTSGILANALNKLSKERGLKLSAAARAYGQD
FT                   MDLIKDMNMVILAPQMESMKGNLKKITDKYGVKLVTTTGRQYIELTNNGDMALDFVESN
FT                   L"
XX
SQ   Sequence 2100 BP; 536 A; 470 C; 486 G; 608 T; 0 other;
     ctgcagcgtg ttgatacata tgccgttacc attgtccctg aggttgccac tgaacttaag        60
     caaaactatc cgcagtctta caagattgcc tcagaaattt tcgatgaaat taaggatcaa       120
     ctctatcgca gtatgagtga ggacgaacga ctttacttca tcatccacat tcagcgattg       180
     ataaacgaag caccagccca gaatcattca caaaacgatt cattataacg cgctcgcagt       240
     cgtagaagcc tacacataag ggctttgaag caatctacca aagattgggc cagtttgctt       300
     caagcacgct tatactttgg cttccaagcg ctcaggagga aaagactcat gaataaggtt       360
     tttgataaat taaaaccggt ttttgaagcc atcgctgcta acaaatatat ttccgcgatt       420
     cgtgatggct ttatcgcatg tatgccgatc atcatcttct caagtatctt tatgatggtt       480
     gcttatgttc cgaacgcttg ggggttttac tggccagaca acgtaacgaa tacgttgatg       540
     gttgcttata actattccat gggcttgctg gcactgtttg tggcgggaac gactgctaaa       600
     aacttgactg acagcaagaa ccttgaatta ccgaaaacaa atcaaattaa tccagtggcc       660
     gtcattgtgg cgtctgaaat ttcttttgtc attttgtcga tcttgccgct taaaaccggt       720
     gttgacctga cttacatggg gactcaggga ttgatctgcg cttatatcgt tgggttgatc       780
     gtgccaaata tttactacgt ctgtattaag aacaatgtca cgattaaatt accagagcag       840
     gtaccaggta atatcgccca gtcatttaaa gaccttatcc cgatgggcct ttctgtgaca       900
     gccttctggc ttttcggtgt tggcttcaaa gcggcaacgg gtactgtatt gccacgctgg       960
     atcattcaag tactttcgcc gcttttccaa gccagcgatt cttatctagg cttagccctg      1020
     attgctggcg cgatggcttt cttctggttc tgtggtgtcc aaggcccatc aattgttcag      1080
     ccggctgttg tcccgattat gattgcgaat acagcagcta acttgcagca atatcaggca      1140
     gggcaacatg tctcgcacgt tttggccatg aacacgatgg actacgtcat gaattttggt      1200
     ggaaccggag caacattggt ggtacctttc atcatgctgt ttgcggcccg atcagcgcaa      1260
     ttaaaagccg ttggtaaggc tgcctttgtg ccatgcactt tcggggtcaa tgaacctgtt      1320
     ttgttcggaa tgccgattat catgaatccc atgttattca ttccgttctt agcaaccccg      1380
     atcgtgaacg tttgcttatt caaattcttt gtgagtgtac ttggcatgaa cagtatgatg      1440
     tacaccatgc catggacagt tccgggaccg attgggattt tgatttcgac tgggtttgcc      1500
     ccgttggctt tcgtctttgt tctactgacg ttggttctgg acgttgcgat atacttccca      1560
     ttcattcgcg tttatgacag cacactctta gctgaagaaa aagctaagga agaggtcatt      1620
     gaagacgacg gcatggctgt tctggcgagt gacacagtgt caccttctat cccgactggt      1680
     ttgactgttg cgactgccac agacgacgat gcgacccatg tgttgcctga aacagcccca      1740
     tctgcccacg gtgaagccta tttcaaacaa aatgaagttg atgttttagt gttgtgtgct      1800
     ggtggtggaa caagcggtat tcttgccaac gcgctaaaca aactgtctaa ggaacgcggt      1860
     ttaaagctct cggccgccgc ccgcgcttat ggacaggata tggatttgat caaagatatg      1920
     aacatggtca tccttgcgcc gcaaatggaa agcatgaaag gtaatttaaa aaagatcacc      1980
     gataagtatg gcgtcaagct ggtcacaaca actggtcggc aatacattga attaaccaac      2040
     aatggtgaca tggcacttga ttttgttgaa tccaatcttt gagacactta acaggaggtt      2100
//