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EBI DbfetchID M60403; SV 1; linear; genomic DNA; STD; PLN; 4699 BP. XX AC M60403; XX DT 24-JUL-1991 (Rel. 28, Created) DT 14-NOV-2006 (Rel. 89, Last updated, Version 10) XX DE Nicotiana tabacum glucan beta-1,3-glucosidase gene, complete cds. XX KW beta-1,3-glucanase; glucan beta-1,3-glucosidase. XX OS Nicotiana tabacum (common tobacco) OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; OC Spermatophyta; Magnoliophyta; eudicotyledons; core eudicotyledons; OC asterids; lamiids; Solanales; Solanaceae; Nicotianoideae; Nicotianeae; OC Nicotiana. XX RN [1] RP 1-4699 RX DOI; 10.1073/pnas.88.5.1820 RX PUBMED; 2000389. RA Sperisen C., Ryals J., Meins F.; RT "Comparison of cloned genes provides evidence for intergenomic exchange of RT DNA in the evolution of a tobacco glucan endo-1,3-beta-glucosidase gene RT family"; RL Proc. Natl. Acad. Sci. U.S.A. 88(5):1820-1824(1991). XX DR EPD; EP35044; NT_E13F. XX FH Key Location/Qualifiers FH FT source 1..4699 FT /organism="Nicotiana tabacum" FT /strain="Havana 425" FT /mol_type="genomic DNA" FT /clone="lambda-GLB" FT /tissue_type="leaf" FT /db_xref="taxon:4097" FT TATA_signal 1597..1606 FT /gene="glucanase GLB" FT exon 1632..1738 FT /gene="glucanase GLB" FT /number=1 FT mRNA join(1632..1738,2486..3991) FT /gene="glucanase GLB" FT /product="glucan-1,3-beta-glucosidase" FT /note="major transcription start site" FT sig_peptide join(1648..1738,2486..2490) FT /gene="glucanase GLB" FT CDS join(1648..1738,2486..3507) FT /codon_start=1 FT /gene="glucanase GLB" FT /product="glucan-1,3-beta-glucosidase" FT /EC_number="3.2.1.39" FT /db_xref="GOA:P27666" FT /db_xref="HSSP:1GHS" FT /db_xref="InterPro:IPR013781" FT /db_xref="UniProtKB/Swiss-Prot:P27666" FT /protein_id="AAA63540.1" FT /translation="MSTSDKHNTPQMAAITLLGLLLVASTIEIAGAQSIGVCYGMLGNN FT LPNHWEVIQLYKSRNIGRLRLYDPNHGALQALKGSNIEVMLGLPNSDVKHIASGMEHAR FT WWVQKNVKDFWPDVKIKYIAVGNEISPVTGTSYLTSFLTPAMVNIYKAIGEAGLGNNIK FT VSTSVDMTLIGNSYPPSQGSFRNDARWFTDPIVGFLRDTRAPLLVNIYPYFSYSGNPGQ FT ISLPYSLFTAPNVVVQDGSRQYRNLFDAMLDSVYAALERSGGASVGIVVSESGWPSAGA FT FGATYDNAATYLRNLIQHAKEGSPRKPGPIETYIFAMFDENNKNPELEKHFGLFSPNKQ FT PKYNLNFGVSGGVWDSSVETNATASLISEM" FT intron 1739..2485 FT /gene="glucanase GLB" FT exon 2486..3991 FT /gene="glucanase GLB" FT /number=2 FT mat_peptide 2491..3438 FT /gene="glucanase GLB" FT /product="glucan-1,3-beta-glucosidase" FT /EC_number="3.2.1.39" FT polyA_signal 3703..3708 FT /gene="glucanase GLB" XX SQ Sequence 4699 BP; 1509 A; 857 C; 786 G; 1547 T; 0 other; gagctccctt ggggggcaag ggcaaaactt tttgctaaat ggaaaaatat tataccaagt 60 gtttgtaata gttactcaat ttgaattaac aaaggggcaa atttgactat tttgccctta 120 tatcttttgg tcacaaaaac ataaaatatc ccatccgaaa ttccaaatgg tccattatcg 180 gcaagtagct ttctttaatt atagttagtt gacaaaacac tatcaagata tcattattat 240 aataataact tcaaagtcca tcatcttagc tgcctcctca gtagagccgc cagtaaaata 300 agaccgatca aataaaagcc gccattaaaa taatgaattt taggactctc gattggcacg 360 taagtgccaa aactcttcca atactttgct gcaacttggg gctgctaggt tctgagcttc 420 cagatatggg atatttctaa gtttatctcc taatttacat ctcaactaat attaagaaat 480 taaacaggta cagcaaatca taaaattttc ctctaaagaa gacaatgaat ccggttactg 540 attcattggc cttttcagag tctgcatgcc atattcacta aggggtcgtt tggtacaaga 600 aataataata ataatttcgg gatagaattt gagattgcat ttatcttgtg tttaattata 660 agtattagct aatttcagaa taaattttac actaaaatag taaaatcaac tatcacatgt 720 agaaggtgga atggaatagc taatcccata gccactcaca tagaatatcc ttatttatct 780 cactatttta ccaaatgatc ggttagtctt catgagaatc cagtatcctc aataaatgca 840 gtaagaagtt agaaaatttt cattaaatca attcatataa tttaaaaata ttagatatgg 900 agcacttaag atacaataaa agatgtaccg ttaataataa aagataagat agagttttaa 960 ataggaaaaa aaaaacggtt cgagacactc ttatggaagg cgttgtcttc aaagtagatt 1020 ctcattcatt gctctggtgc aatagcaaaa tgacatctta ctcttaagat acagcgagcc 1080 actctacaat cttctattgt atactcaaat gaaagtttta gagaactttc aaatctctca 1140 actactttta agggaattca aaatacgacc aatatttatt acttacttac ttatagttaa 1200 atgatatgaa ttttatttta aatttgaatt gaaaatatta aattacttga tttaatataa 1260 acaatagata tcgctaagta tttaccacaa acatggagat actacagaag attttattat 1320 ttgtaacgat gattaagcag ctattcatct ggttgtgcag gatgaaagaa agtaactagc 1380 tataatttct tttgtaaagt caagatagta cggcaccttt ggagaaaata taaaacttgt 1440 acatcatcaa actcattttc ttttttccac aaaatgatca agttgacatg ttaatagcca 1500 ggtcagccgg ggcggctctt aacttcatta gcctacgaat aacaaatact ccaataatat 1560 tccactcgaa tatttacatt tacacaaggc tatctcaata taaatagctc attgttcatc 1620 ttaattcttc caacaagtct tctcatcatg tctacctcag ataaacataa tactcctcaa 1680 atggctgcta tcacactgct aggattacta cttgttgcca gcaccattga gatagcaggt 1740 ttctggtcaa atatttgaac ttccgagcca aaaatattgt cttataattt tgttggtgca 1800 aaatcttaat ttagttgata gttatttgct tatttttctt atcaaattgc ttgtgttttt 1860 ttctcaaatt tacttgcact atattcattt agcgatagtt atttgctctt ttttcgggta 1920 acactcactc acaagctttt caaatttgag gggagggcgg tgaatctaaa atttgaaatt 1980 tatgagttta gactagtgtc catttgattt attggctaga cgtctattag ttgtatagta 2040 aatcttttaa cacatacacc gacctgagtc aaagctatta ggttcgtatt aacacataac 2100 acatattccc tctgttctaa tttatgtgac acactttctg ttagtttctt ccaaagagaa 2160 tgacatattt gatatttaaa aatattttaa ctttaaactt tttattctca accttttata 2220 acatcacaaa tattatggaa catattagac tacaagttcc aaatatctta tagtctgtac 2280 aaatattata gcatgtttaa taacacaatt ttcacattct tctttttctt aaactttgtg 2340 ccgaattaaa ttatgtcaca taaattaaaa tggttacatc atcccctagt ggagggacct 2400 accatgtcta ctgtaagttt ttaacttttc aagaattaca taattgattt agtttctaac 2460 actaattcta attcttattg agcaggggct caatcaatag gtgtttgcta tggaatgcta 2520 ggcaacaact tgccaaatca ttgggaagtt atacagctct acaagtcaag aaacatagga 2580 agactgaggc tttatgatcc aaatcatgga gctttacaag cattaaaagg ctcaaacatt 2640 gaagttatgt taggacttcc caattcagat gtcaagcaca ttgcttccgg aatggaacat 2700 gcaagatggt gggtacaaaa aaatgttaaa gatttctggc cagatgttaa gattaagtat 2760 attgctgttg ggaatgaaat cagccctgtc acaggcacat cttaccttac ctcatttctt 2820 actcctgcca tggtaaatat ttacaaagca attggtgaag ctggtttagg aaacaacatc 2880 aaggtctcaa cttctgtaga catgaccttg attggaaact cttatccacc atcacagggt 2940 tcgtttagga acgatgctag gtggtttact gatccaattg ttgggttctt aagggacaca 3000 cgtgcacctt tactcgttaa catttacccc tatttcagct attctggtaa tccagggcag 3060 atttctctcc cctattctct ttttacagca ccaaatgtgg tagtacaaga tggttcacgc 3120 caatatagga acttatttga tgcaatgctg gattctgtgt atgctgccct cgagcgatca 3180 ggaggggcat ctgtagggat tgttgtgtcc gagagtggct ggccatctgc tggtgcattt 3240 ggagctacat atgacaatgc agcaacttac ttgaggaact taattcaaca cgctaaagag 3300 ggtagcccaa gaaagcctgg acctattgag acctatatat ttgccatgtt tgatgagaac 3360 aacaagaacc ctgaactgga gaaacatttt ggattgtttt cccccaacaa gcagcccaaa 3420 tataatctca actttggggt ctctggtggt gtttgggaca gttcagttga aactaatgct 3480 actgcttctc tcataagtga gatgtgagat gagacacttg aaatctcttt acataagtat 3540 tgcttagatg gaaagcttag taaaaacaag agaaatttat tcttcatgca agacactaaa 3600 taacattgca cgtctctgta agtcctcatg gattgttatc cagagacgat gcaactctga 3660 gtggttttaa atcccttttc tttgtgatgt tggtaatttg gcaagaaact ttctgtaagt 3720 ttgtgaattt catgactttc tgtaagtttg tgaatttcat gcaccatcaa ttatacatct 3780 tttccatgtt tgatcacatt aggatttggt aattgcaaag tttagttatt tattaattag 3840 tgtctttatc atttgactcg atcaattaat ctttaattgg taaggcttga tatatcggag 3900 cgactcttag gtagtggcat tcaactgatc tttctgttat cccatgtctc cgttaaccct 3960 cattagaaat atattataat gtcacctaaa tcagaggttt tagagcttca aaatcgattg 4020 acaacagttt tggagttaca ctgtggttta ggacattctg ttacattata aggtatttca 4080 cgtcgtatca aggtcgaata aaataatggt acgctctttc ttatcacaaa tttctctcaa 4140 cttctagacc aattgaatct tgtctccaat aagtattgct tttactctat gtttctctct 4200 atcaattcaa ggctcaagcc atcaaatcgc ttggtatttc tcgctctcaa ttaaccaatc 4260 gagctgttaa ctctgctaaa gtctcattct tcagcttctc aaaccactca aagcttctcc 4320 aacaaaaaaa gtagaaaaaa actttgatcc acatgtaaaa ccccataacc atgtcattcg 4380 agtgaaggaa ggtgcttgaa atgcaacgaa caaacacggc tttgccactg cttgtgatag 4440 gtaaaagaaa acttgattag atagttcgtg cggggcattt cctacctcgc ctttgatctt 4500 agctttaggc ttgcgaaact aagtagaacc taaggcccca gcgaatccgt aaatctgagg 4560 agcatgccac agcaaaaagg ttagctttca gataagagat ctatttgatc ttacccacta 4620 actagctctg tgtgaattgt ccatttcatc aactccaaag gcaggaaaga agactattga 4680 gagagcagca aaagagctc 4699 // |
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