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EBI DbfetchID M59852; SV 1; linear; genomic DNA; STD; PRO; 3710 BP. XX AC M59852; XX DT 04-APR-1991 (Rel. 28, Created) DT 04-MAR-2000 (Rel. 63, Last updated, Version 4) XX DE A.tumefaciens beta-D-glucosidase (cbg-1) gene, complete cds. XX KW beta-D-glucosidase. XX OS Agrobacterium tumefaciens OC Bacteria; Proteobacteria; Alphaproteobacteria; Rhizobiales; Rhizobiaceae; OC Rhizobium/Agrobacterium group; Agrobacterium. XX RN [1] RP 1-3710 RX PUBMED; 1537792. RA Castle L.A., Smith K.D., Morris R.O.; RT "Cloning and sequencing of an Agrobacterium tumefaciens beta-glucosidase RT gene involved in modifying a vir-inducing plant signal molecule"; RL J. Bacteriol. 174(5):1478-1486(1992). XX FH Key Location/Qualifiers FH FT source 1..3710 FT /organism="Agrobacterium tumefaciens" FT /mol_type="genomic DNA" FT /cell_line="B3/73" FT /tissue_lib="genomic HindIII cosmid" FT /db_xref="taxon:358" FT RBS 169..173 FT /gene="cbg1" FT CDS 181..2637 FT /codon_start=1 FT /transl_table=11 FT /gene="cbg1" FT /product="beta-D-glucosidase" FT /EC_number="3.2.1.21" FT /db_xref="GOA:P27034" FT /db_xref="InterPro:IPR001764" FT /db_xref="InterPro:IPR002772" FT /db_xref="InterPro:IPR011658" FT /db_xref="InterPro:IPR017853" FT /db_xref="InterPro:IPR019800" FT /db_xref="UniProtKB/Swiss-Prot:P27034" FT /protein_id="AAA22082.1" FT /translation="MIDDILDKMTLEEQVSLLSGADFWTTVAIERLGVPKIKVTDGPNG FT ARGGGSLVGGVKSACFPVAIALGATWDPELIERAGVALGGQAKSKGASVLLAPTVNIHR FT SGLNGRNFECYSEDPALTAACAVAYINGVQSQGVAATIKHFVANESEIERQTMSSDVDE FT RTLREIYLPPFEEAVKKAGVKAVMSSYNKLNGTYTSENPWLLTKVLREEWGFDGVVMSD FT WFGSHSTAETINAGLDLEMPGPWRDRGEKLVAAVREGKVKAETVRASARRILLLLERVG FT AFEKAPDLAEHALDLPEDRALIRQLGAEGAVLLKNDGVLPLAKSSFDQIAVIGPNAASA FT RVMGGGSARIAAHYTVSPLEGIRAALSNANSLRHAVGCNNNRLIDVFSGEMTVEYFKGR FT GFESRPVHVETVEKGEFFWFDLPSGDLDLADFSARMTATFVPQETGEHIFGMTNAGLAR FT LFVDGELVVDGYDGWTKGENFFGTANSEQRRAVTLGAARRYRVVVEYEAPKASLDGINI FT CALRFGVEKPLGDAGIAEAVETARKSDIVLLLVGREGEWDTEGLDLPDMRLPGRQEELI FT EAVAETNPNVVVVLQTGGPIEMPWLGKVRAVLQMWYPGQELGNALADVLFGDVEPAGRL FT PQTFPKALTDNSAITDDPSIYPGQDGHVRYAEGIFVGYRHHDTREIEPLFPFGFGLGYT FT RFTWGAPQLSGTEMGADGLTVTVDVTNIGDRAGSDVVQLYVHSPNARVERPFKELRAFA FT KLKLAPGATGTAVLKIAPRDLAYFDVEAGRFRADAGKYELIVAASAIDIRASVSIHLPV FT DHVMEP" FT misc_feature 213..223 FT /gene="cbg1" FT /note="active site" XX SQ Sequence 3710 BP; 718 A; 1084 C; 1168 G; 740 T; 0 other; cgatcggctc gggacacgga cagaattact cactgctcta tgccttggcg gcggtcagtg 60 cctttttggg cgcgctggca atcgtgccgg tacgtggcgc acgttgataa gatcaatatt 120 tcgggcggga acgcctgcct aatggtcccc atcggaccga ttaacatggg aggatcccct 180 atgatcgacg atattctcga taagatgaca ctggaggagc aggtgtcgct gctctcgggc 240 gcggatttct ggacgaccgt cgcgatcgag cggctcggcg tgccgaagat caaggttacc 300 gacggcccca atggcgcacg cggcggcggg tcgctggtcg gcggcgtcaa gtccgcctgc 360 ttcccggtgg caatcgcgct tggagcgacg tgggacccgg agctcatcga gcgcgccggc 420 gtggcgctgg gaggacaagc caagagcaag ggcgcgtcgg tgcttctggc gccgaccgtc 480 aacattcacc gctccggcct caatggccgc aacttcgaat gctattcgga agacccggcg 540 ctgaccgccg cctgcgccgt cgcctatatc aatggcgtgc agagccaggg tgtggccgcc 600 acgatcaagc acttcgtcgc caacgagtcc gagatcgagc ggcagaccat gtcctccgat 660 gtcgatgagc ggacgctgcg cgaaatctat ctgccgcctt tcgaggaggc ggtgaagaag 720 gccggcgtga aggccgtcat gtcctcctac aacaagctca acggcaccta tacgagcgaa 780 aatccctggc tgctgacgaa agtcctgcgc gaggaatggg gcttcgacgg cgtggtcatg 840 tccgactggt tcggctcgca ctcgacggct gaaaccatca atgccgggct cgatctggag 900 atgccggggc cttggcggga tcgcggcgaa aagctggtcg ccgcagtccg ggaaggcaag 960 gtaaaggccg agaccgtgcg cgcttcggca cggcgtattc tccttctgct cgaacgcgtc 1020 ggcgcctttg aaaaggcgcc tgatctcgcc gaacacgcgc ttgatctgcc ggaagatcgt 1080 gcgctcatcc gccaactcgg tgcggagggt gctgtactcc tgaagaatga cggagtgctg 1140 ccgcttgcca agtcgtcctt cgaccagatc gccgtcatcg gccccaatgc ggcttccgca 1200 cgcgtcatgg gcggaggaag cgcgcggatt gccgcgcatt atacggtgag cccacttgag 1260 ggcattcgcg cggcgctgtc caacgccaac agcctccgcc atgcggtcgg ctgtaacaac 1320 aaccggctca tcgacgtctt cagcggcgag atgacggtgg aatacttcaa gggacgcggc 1380 ttcgagagcc gtccggtcca tgtcgagacc gtcgaaaagg gcgaattctt ctggttcgat 1440 cttccgtccg gcgaccttga tctcgccgat ttttcggcgc gcatgacggc gaccttcgtg 1500 ccgcaggaaa ccggtgaaca catcttcggc atgaccaatg ctgggcttgc tcggctgttc 1560 gtggacggcg aactggtggt cgatggctat gacggttgga cgaagggtga gaactttttt 1620 ggaaccgcga acagcgagca gcgtcgggcg gtaacgcttg gggccgcacg ccgctaccgg 1680 gttgtggtcg aatatgaggc gccgaaggcc agcctggacg gcatcaacat atgtgcgctc 1740 cgcttcggtg tcgaaaagcc gctcggcgat gccgggattg cggaggcggt cgaaaccgcc 1800 cgcaagtccg atatcgtact gctcctcgtc ggccgtgagg gcgagtggga caccgaaggt 1860 ctggatctgc ccgacatgcg cctgccgggt cgccaggagg agctgatcga ggcggtcgcc 1920 gaaaccaatc ccaacgtggt cgtggtactg caaacgggtg gtcccatcga gatgccatgg 1980 ctcggcaagg tgcgtgcggt gctgcagatg tggtatcccg gccaggaact tggcaatgcg 2040 cttgcggacg ttctctttgg tgatgtcgag cctgccggcc gcttgccaca gaccttcccg 2100 aaggcgctca cggataattc cgccattacc gacgatccgt cgatctatcc tggccaggac 2160 ggccatgtgc gctacgcgga agggatcttc gtcggctatc gccatcacga tacaagagag 2220 atcgaaccac tcttcccctt cggcttcggt cttggctaca cccgctttac ctggggtgcc 2280 ccgcaactat cgggaacgga aatgggggcg gatggtctta cggtgacggt cgatgtcacc 2340 aatataggcg acagggcggg atcggacgtg gtgcagctct atgttcactc tcccaatgcc 2400 agggtcgagc ggccgttcaa ggagctgcgt gcctttgcga agctcaagct ggccccgggc 2460 gcgaccggta cggcggtgct gaagatcgct cctcgcgact tggcttactt cgatgtcgag 2520 gccggtcgtt tccgggctga tgcgggcaag tacgagctga tcgtggcggc cagcgccatc 2580 gatatccggg caagcgtaag tattcacttg ccggtcgatc atgtgatgga gccgtagcgc 2640 aagcattcaa tatgagccgc gccgcctatc ttagaaggag gcggcgcgat ccgcgaagtg 2700 agtgggacag ggcctggcga tcgctctctt aatgtacata cctgggatgt atatttccag 2760 gccgatgcgc tatgatacat ccatgtacac acatgggagc atcccgctat gcagatgacc 2820 aaggcattca agaacggcaa ttcccaagcc gtacgcatcc cagcagagat tgcctatgag 2880 cgcacggaca tggagctgga gattgagcgg gtaggcgatg aaatccgcat tcgtcctgtc 2940 cgccagtcgt tggccggcgt catggatgtt ttcgcacgct tttcccccaa tttcatgagc 3000 gagggcaggg gtgaccaaga gcaggacgag cgcgagagaa gatctgatgc cgcgctatat 3060 gctcgatacg aatatgtgca tctatctgat gaagaaccag cccgagcaag tcgctcgtcg 3120 ttttgccgct tgctacgtcg gtgacgtggt gatgtcggcc attaccttcg cggagctgga 3180 ttacggtgtt gccgcgtctg atgccccgga acgggagcgc ggcaaccttg atgccttggt 3240 gaccctcatt gcgtagagcc gttcgatatc gccgctgccc acgcttatgg cccagtacgc 3300 atggcaacgc gagagcaaga aagaccatct gacagctgat cgcatcgcgc tcattgcctt 3360 gatacgtgct gtaccacaac acacgagatt catgctatcc actcaatcga actgctgata 3420 ctgaggctgc gttcgtgctt acggcagcgc cgactaaagg aaccatcgtc ccttagtcat 3480 ctcagatcca tgccttcatc ggcattgcga tcatgacaca cggctcattt cagacttgga 3540 aacagcctag aacaaccgct ccagccagcc gtgcttgtcg ggcgcacgtc cgttttgaat 3600 gtcgaggagt gccgccttca ggcgcgtggt caccggaccg gcgccgccgt cgccaatggt 3660 gaaattgtgc ttgcggccct tgagcttgcc gatcggagtc accactgcag 3710 // |
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