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EBI DbfetchID M57676; SV 1; linear; genomic DNA; STD; PRO; 6634 BP. XX AC M57676; M32130; XX DT 12-FEB-1991 (Rel. 27, Created) DT 31-AUG-2006 (Rel. 89, Last updated, Version 9) XX DE Bacillus subtilis hemAXCDBL gene cluster. XX KW aminolevulinic acid dehydratase; KW glutamate-1-semialdehyde 2,1-aminotransferase; KW NAD(P)H:transfer RNA-Gln reductase; porphobilinogen deaminase; KW uroporphyrinogen III synthase. XX OS Bacillus subtilis OC Bacteria; Firmicutes; Bacillales; Bacillaceae; Bacillus. XX RN [1] RX PUBMED; 2110138. RA Petricek M., Rutberg L., Schroeder I., Hederstedt L.; RT "Cloning and characterization of the hemA region of the Bacillus subtilis RT chromosome"; RL J. Bacteriol. 172(5):2250-2258(1990). XX RN [2] RP 1-6634 RX PUBMED; 1672867. RA Hansson M., Rutberg L., Schroder I., Hederstedt L.; RT "The Bacillus subtilis hemAXCDBL gene cluster, which encodes enzymes of the RT biosynthetic pathway from glutamate to uroporphyrinogen III"; RL J. Bacteriol. 173(8):2590-2599(1991). XX FH Key Location/Qualifiers FH FT source 1..6634 FT /organism="Bacillus subtilis" FT /mol_type="genomic DNA" FT /db_xref="taxon:1423" FT CDS 232..1599 FT /codon_start=1 FT /transl_table=11 FT /gene="hemA" FT /product="NAD(P)H:glutamyl-transfer RNA reductase" FT /note="The product of this hemA gene is not an FT 5-aminolevulinic acid synthase" FT /db_xref="GOA:P16618" FT /db_xref="HSSP:1GPJ" FT /db_xref="InterPro:IPR006151" FT /db_xref="UniProtKB/Swiss-Prot:P16618" FT /protein_id="AAA22510.1" FT /translation="MHILVVGVDYKSAPIEIREKVSFQPNELAEAMVQLKEEKSILENI FT IVSTCNRTEIYAVVDQLHTGRYYIKKFLADWFQLSKEELSPFLTFYESDAAVEHLFRVA FT CGLDSMVIGETQILGQVRDSFKTAQQEKTIGTIFNELFKQAVTVGKRTHAETDIGSNAV FT SVSYAAVELAKKIFGNLSSKHILILGAGKMGELAAENLHGQGIGKVTVINRTYLKAKEL FT ADRFSGEARSLNQLESALAEADILISSTGASEFVVSKEMMENANKLRKGRPLFMVDIAV FT PRDLDPALNDLEGVFLYDIDDLEGIVEANMKERRETAEKVELLIEETIVEFKQWMNTLG FT VVPVISALREKALAIQSETMDSIERKLPHLSTREKKLLNKHTKSIINQMLRDPILKVKE FT LAADADSEEKLALFMQIFDIEEAAGRQMMKTVESSQKVHSFKKAESKAGFSPLVSE" FT CDS 1607..2437 FT /codon_start=1 FT /transl_table=11 FT /gene="hemX" FT /note="unidentified gene product" FT /db_xref="GOA:P16645" FT /db_xref="InterPro:IPR002541" FT /db_xref="UniProtKB/Swiss-Prot:P16645" FT /experiment="experimental evidence, no additional details FT recorded" FT /protein_id="AAA22511.1" FT /translation="MIDTAMARLNEGTIVIYALSVLFYFIDFLQHNRKAGKMAFWLLSI FT VWTLQTVYLAYFMWVTGRFPVLNVTEALYFYAWVLVTLSLVLTKLLRVDFIVFFTNVIG FT FSMIAIHTFSPTEQQSAAFSGQLVSELLVIHITMAILSYGAFSLSFVFSVLYMFQYHVL FT KKKKWGKWLLRIEDLSKLDYMAYVLNVIGVPMLLLSLILGVIWAYVSLETLYWFDAKVL FT GSFVVLLLYSYYLYIRLIKELQGKVAALWNTACFLVLMINYFLLGSLSQFHWFS" FT CDS 2470..3414 FT /codon_start=1 FT /transl_table=11 FT /gene="hemC" FT /product="porphobilinogen deaminase" FT /function="hydroxymethylbilane synthesis from FT porphobilinogen" FT /EC_number="4.3.1.8" FT /db_xref="GOA:P16616" FT /db_xref="HSSP:1PDA" FT /db_xref="InterPro:IPR000860" FT /db_xref="UniProtKB/Swiss-Prot:P16616" FT /experiment="experimental evidence, no additional details FT recorded" FT /protein_id="AAA22512.1" FT /translation="MMRTIKVGSRRSKLAMTQTKWVIQKLKEINPSFAFEIKEIVTKGD FT RIVDVTLSKVGGKGLFVKEIEQALLNEEIDMAVHSMKDMPAVLPEGLVIGCIPEREDPR FT DALISKNRVKLSEMKKGAVIGTSSLRRSAQLLIERPDLTIKWIRGNIDTRLQKLETEDY FT DAIILAAAGLSRMGWKQDVVTEFLEPERCLPAVGQGALAIECRESDEELLALFSQFTDE FT YTKRTVLAERAFLNAMEGGCQVPIAGYSVLNGQDEIEMTGLVASPDGKIIFKETVTGND FT PEEVGKRCAALMADKGAKDLIDRVKRELDEDGK" FT CDS 3404..4192 FT /codon_start=1 FT /transl_table=11 FT /gene="hemD" FT /product="uroporphyrinogen III synthase" FT /EC_number="4.2.1.24" FT /db_xref="GOA:P21248" FT /db_xref="InterPro:IPR003754" FT /db_xref="UniProtKB/Swiss-Prot:P21248" FT /experiment="experimental evidence, no additional details FT recorded" FT /protein_id="AAA22513.1" FT /translation="MENDFPLKGKTVLVTRNKAQAASFQQKVEALGGKAVLTSLITFRR FT ALPNDVAEQVREDLAAPGWLVFTSVNGADFFFSYLKENQLILPAHKKIAAVGEKTARRL FT KMHNVSVDVMPQEYIAEQLADALKQHAEPGETITVMKGNLSRDVIKQELVPLGFEVKEW FT VLYETIPDEEGIEALKDAAGQYSFDYVTFTSSSTVHTFMHVLGEELKKWKANGTACISI FT GPLTNDALLTYGITSHTPDTFTIDGMLELMCSMSREEERI" FT CDS 4189..5163 FT /codon_start=1 FT /transl_table=11 FT /gene="hemB" FT /product="aminolevulinic acid dehydratase" FT /function="porphobilinogen synthesis" FT /EC_number="4.2.1.24" FT /db_xref="GOA:P30950" FT /db_xref="HSSP:1B4K" FT /db_xref="InterPro:IPR013785" FT /db_xref="UniProtKB/Swiss-Prot:P30950" FT /experiment="experimental evidence, no additional details FT recorded" FT /protein_id="AAA22514.1" FT /translation="MSQSFNRHRRLRTSKAMREMVKETRLHPSDFIYPIFVVEGLEGKK FT AVPSMPDVHHVSLDLLKDEVAELVKLGIQSVIVFGIPEEKDDCGTQAYHDHGIVQKAIT FT EIKEHFPEMVVVADTCLCEYTDHGHCGLVKDGVILNDESLELLAQTAVSQAKAGADIIA FT PSNMMDGFVTVIREALDKEGFVNIPIMSYAVKYSSEFYGPFRDAANSTPQFGDRKTYQM FT DPANRMEALREAQSDVEEGADFLIVKPSLSYMDIMRDVKNEFTLPLVAYNVSGEYSMVK FT AAAQNGWIKEKEIVLEILTSMKRAGADLIITYHAKDAAKWLAE" FT CDS 5193..6485 FT /codon_start=1 FT /transl_table=11 FT /gene="hemL" FT /product="glutamate-1-semialdehyde 2,1-aminotransferase" FT /function="5-aminolevulinic acid synthesis" FT /EC_number="5.4.3.8" FT /db_xref="GOA:P30949" FT /db_xref="InterPro:IPR015421" FT /db_xref="PDB:3BS8" FT /db_xref="UniProtKB/Swiss-Prot:P30949" FT /experiment="experimental evidence, no additional details FT recorded" FT /protein_id="AAA22515.1" FT /translation="MRSYEKSKTAFKEAQKLMPGGVNSPVRAFKSVDMDPIFMERGKGS FT KIFDIDGNEYIDYVLSWGPLILGHTNDRVVESLKKVAEYGTSFGAPTEVENELAKLVID FT RVPSVEIVRMVSSGTEATMSALRLARGYTGRNKILKFEGCYHGHGDSLLIKAGSGVATL FT GLPDSPGVPEGIAKNTITVPYNDLESVKLAFQQFGEDIAGVIVEPVAGNMGVVPPQEGF FT LQGLRDITEQYGSLLIFDEVMTGFRVDYNCAQGYFGVTPDLTCLGKVIGGGLPVGAYGG FT KAEIMEQIAPSGPIYQAGTLSGNPLAMTAGLETLKQLTPESYKNFIKKGDRLEEGISKT FT AGAHGIPHTFNRAGSMIGFFFTNEPVINYETAKSSDLKLFASYYKGMANEGVFLPPSQF FT EGLFLSTAHTDEDIENTIQAAEKVFAEISRR" XX SQ Sequence 6634 BP; 1894 A; 1273 C; 1669 G; 1798 T; 0 other; atgcatatca ccttcttgtt ttttagagct gatgtgtagt aaatttctgc tgtttttggt 60 attgtcaata ggaatgcttc ttttccctga agctttttct aatatagcat aagaatttta 120 aaatctgttc acattttgtg aaagaaacta tgttataatt attataaata atgagttcta 180 tgttagaatg attataaatt aagattgggt gttgggggtg taattagagc gatgcatata 240 cttgttgtgg gagtagatta taaatccgcc cctattgaga tacgtgaaaa agtaagtttt 300 cagccgaatg agctggcaga agcaatggtg cagctgaaag aagagaaaag cattcttgaa 360 aacatcattg tctcaacctg caaccgcact gaaatttatg cggtagtcga ccagcttcat 420 accggccgtt attatataaa aaagttttta gctgattggt ttcaattaag caaagaagag 480 ctgtcaccgt tcttaacgtt ttatgagagc gatgccgctg ttgagcattt attccgtgta 540 gcctgcggac ttgattctat ggtgattggc gaaacgcaga ttctcggaca ggtacgcgac 600 agctttaaaa cagctcagca agaaaaaacg atcgggacta tttttaatga gctgtttaag 660 caggcagtta cagtgggcaa acggactcac gccgaaacag acattggctc aaatgcggtg 720 tcagtaagct atgctgcagt tgaacttgcc aaaaaaatct tcggaaatct ttcaagcaag 780 cacatattga ttctcggtgc gggaaaaatg ggcgagcttg ctgcggaaaa cctgcacgga 840 cagggaatcg gcaaggtcac tgtcattaac cgaacatact tgaaagcgaa ggagcttgca 900 gaccgttttt caggtgaagc gagaagcttg aatcagcttg aaagcgcgct tgcggaggct 960 gatattttaa tcagttcaac cggtgcaagt gaatttgtcg tgtccaaaga gatgatggaa 1020 aacgcgaata agcttcgcaa gggacgtccg ctgtttatgg tcgacattgc cgtgcctaga 1080 gatcttgatc cggcgctgaa tgatcttgaa ggtgtttttc tttatgatat cgacgatctg 1140 gaaggcattg tagaagcgaa catgaaagag cggagagaaa cagctgaaaa agttgaactg 1200 ttaattgaag aaaccattgt ggaatttaaa caatggatga atacacttgg tgttgtgcct 1260 gttatttctg cattgcgcga aaaggcgctt gccatccagt cagaaacgat ggacagcatt 1320 gagcgtaagc tgcctcactt aagcacaaga gagaaaaaac tgttgaacaa acacaccaaa 1380 agtattatta accaaatgct tcgtgatccg attttaaagg tgaaagagct tgcggcagat 1440 gctgattctg aagaaaagct cgcgttgttt atgcagattt ttgatattga agaagctgcg 1500 ggccgtcaaa tgatgaaaac cgttgaaagc agccagaagg tccactcttt taagaaggct 1560 gaatcaaaag cgggctttag cccacttgta agtgagtgaa agctgaatga ttgatactgc 1620 aatggcaaga cttaatgagg ggacaatcgt catttacgcg ttaagtgtac tcttttattt 1680 tatagatttt cttcaacaca accggaaggc tggaaaaatg gccttctggt tgctttctat 1740 tgtctggact ctgcaaaccg tgtatttggc ctattttatg tgggtgacgg ggcggtttcc 1800 ggtattaaat gtgacagagg cactttattt ttatgcctgg gtgcttgtca cgctgtcact 1860 tgtactgaca aagcttttac gtgttgactt tatcgtgttt tttacaaatg ttataggatt 1920 ttctatgatc gccattcaca cattttcacc gacagagcag cagtcagctg ctttttccgg 1980 gcagcttgta tccgagcttt tggtgattca tattacaatg gcgattcttt catacggcgc 2040 tttttccctt tctttcgttt tttctgtgct atatatgttt caatatcatg tgctgaaaaa 2100 gaaaaagtgg ggaaaatggc tgttgagaat agaagattta tctaagcttg attatatggc 2160 gtatgtttta aatgtcattg gggttccgat gctgctgctg agtttgattc tcggcgtcat 2220 ttgggcgtat gtctcactag aaacgctgta ttggtttgac gccaaagtgc ttggttcgtt 2280 tgtcgtcctg ctgctgtaca gctattatct ttatatcagg ctgattaagg agctgcaagg 2340 aaaggtcgct gcactgtgga atacggcttg ttttctggtg ctgatgatca attatttcct 2400 gcttggaagc ctgtcgcaat tccattggtt cagttaaacg atgtcccaag cagattcggg 2460 aggaaagaaa tgatgagaac gattaaagta ggttccagac ggagcaaact cgctatgact 2520 caaacaaaat gggttattca aaaactgaag gaaatcaatc cttcgtttgc ttttgaaatt 2580 aaagagatcg tgacaaaggg cgaccggatt gtcgatgtta cactctcaaa agtgggtgga 2640 aaagggcttt ttgtcaaaga aattgaacag gcgcttttaa acgaagagat tgatatggca 2700 gtgcacagca tgaaggacat gcctgctgtt ttgcctgaag gccttgtgat cggctgtatt 2760 cctgaacggg aggacccgcg tgatgccctt atttcaaaga atcgcgtaaa gctttcagaa 2820 atgaagaaag gtgctgtcat tggcacaagc agtttaagaa gaagcgcgca gcttttgatt 2880 gagcgccctg accttacaat taaatggatt agaggtaata ttgatacaag acttcaaaag 2940 ctggaaacag aggattatga cgcaattatt ttagcggctg ccggcctttc cagaatgggt 3000 tggaagcaag atgtcgtaac cgaattcctt gagcctgagc gctgtttgcc tgctgtgggg 3060 cagggagccc tggcgattga gtgccgagaa tcggatgaag agctgttggc gttgttttct 3120 cagtttacag atgaatatac aaaacggact gtcttagcgg aacgtgcttt tttaaacgcg 3180 atggagggcg gctgccaggt tccgatcgcg ggctactccg tgttaaatgg acaggatgaa 3240 attgaaatga caggtcttgt cgcttcacct gacggcaaaa tcatttttaa agaaaccgtc 3300 accggaaacg atccggagga agtaggaaag cgctgtgccg ctcttatggc tgacaaagga 3360 gcaaaagatt taattgatcg tgtaaaacgg gagcttgacg aggatggaaa atgattttcc 3420 gttgaaagga aaaacagtgc ttgtcacccg gaataaggca caggcagcat catttcagca 3480 aaaagtggag gcgcttggcg gtaaagcggt tttaacctct ttgattacgt ttcgccgcgc 3540 tttgccgaat gatgttgcgg aacaggtaag agaggatctt gccgcgccag gctggcttgt 3600 ttttacaagt gtgaacgggg cagacttctt tttttcttat ctgaaggaaa atcagcttat 3660 tctccctgcg cataaaaaaa ttgcagccgt cggtgaaaaa accgcgcgcc gtttaaaaat 3720 gcataacgta tcggttgatg tgatgccaca ggagtatatt gctgaacaat tggctgacgc 3780 tcttaagcag catgctgaac cgggggagac cattaccgtg atgaaaggga atttgtcacg 3840 tgatgtgata aaacaagagc ttgtcccgct cggttttgaa gtaaaggaat gggttctcta 3900 cgaaacgatt ccggatgaag aaggcattga ggcattgaaa gacgctgcgg gtcaatattc 3960 ctttgactat gtaacattta cgagttcatc aaccgtacat acgtttatgc atgtcttggg 4020 agaagaatta aaaaagtgga aggcgaatgg gacggcctgt atcagcattg ggcctttaac 4080 aaatgatgcc cttctgacgt acggcatcac atcgcatacg cctgatacat ttacaataga 4140 tggcatgctt gagttaatgt gcagcatgtc aagagaggaa gagagaatat gagtcaatca 4200 tttaatagac accgccgcct gcggacatca aaagcaatga gagaaatggt aaaggaaaca 4260 cgtttgcatc catcagattt tatatatccg atttttgtcg ttgaagggct ggaaggcaaa 4320 aaagctgttc cgtcaatgcc tgatgttcac catgtatcat tagatttatt gaaggatgaa 4380 gtagcggagc tggtcaaact gggcattcaa tctgttatcg tgttcggcat cccggaagaa 4440 aaagatgatt gcggaacaca agcgtaccat gatcacggaa ttgtccaaaa agccatcaca 4500 gaaattaaag aacacttccc tgaaatggtt gttgtcgctg acacgtgcct gtgcgaatat 4560 acagaccacg gccattgcgg acttgtcaaa gacggagtca ttctcaatga tgaatcgctg 4620 gagcttttgg cgcagacagc tgtcagccaa gcgaaagcag gtgcggatat cattgcgcca 4680 tcaaacatga tggacggatt tgttacagtg atcagagaag cacttgataa agaaggattc 4740 gtcaatattc ccatcatgtc ttacgctgtt aaatattcaa gtgagtttta cggtccgttc 4800 cgtgatgcag caaacagcac accgcaattc ggagaccgca aaacatatca gatggaccct 4860 gccaaccgta tggaggcact ccgcgaagca caatcagatg ttgaggaagg cgcggacttt 4920 ttgattgtca aaccttcgct ttcttatatg gatatcatgc gtgacgtaaa aaatgagttt 4980 actttgccgc tcgtcgctta taatgtaagc ggagagtatt caatggtgaa ggctgcagcg 5040 cagaacggct ggatcaaaga aaaagaaatt gtgttggaaa ttttgacaag catgaagcgc 5100 gcgggtgccg acctgattat tacgtatcat gcgaaagacg cagcgaaatg gcttgcggag 5160 taattttatt cagttgacag gagttgaggc agatgagaag ctatgaaaaa tcaaaaacgg 5220 cttttaaaga agcgcaaaaa ctcatgccgg gcggtgtgaa cagtcccgtt cgcgcattta 5280 aatcggtaga catggacccg atttttatgg agcgcggaaa aggctcgaaa atctttgata 5340 ttgacgggaa tgaatatatt gactacgtct tgtcatgggg gcctttaatt ttagggcata 5400 caaatgaccg cgtcgtagaa agcctcaaaa aagtggctga atacgggaca agctttggtg 5460 ctccgactga agtagaaaat gaactggcta agctcgtcat tgatcgtgtg ccatctgtag 5520 aaattgtacg aatggtaagc tccggaacag aggctacaat gagtgccctc cgtttggcaa 5580 ggggctatac gggccgcaac aagattttaa aatttgaggg ctgctaccac ggacacggcg 5640 attctctctt gattaaagct ggttcaggtg ttgccactct cggtctgcct gacagcccgg 5700 gggtgcctga aggcattgcg aaaaacacca tcaccgttcc gtacaatgat ttagaaagtg 5760 taaagcttgc tttccagcaa ttcggtgaag acattgcggg agtcattgta gagccagttg 5820 ccggaaatat gggtgttgtt ccgccgcaag aaggtttcct tcagggtctg cgtgatatca 5880 ctgagcagta cggctccctg ctgatttttg atgaagtgat gactggcttc cgggtcgatt 5940 ataactgcgc tcaaggctac ttcggcgtaa cgcctgatct gacttgttta ggaaaagtaa 6000 tcgggggcgg acttcctgtc ggcgcttatg gcggaaaggc agaaatcatg gagcagatcg 6060 ctccaagcgg tccgatctat caagctggta cattgtcagg caacccgctt gcgatgacgg 6120 ctggcttaga gacattgaaa cagctgacac ctgaatccta caagaatttc atcaaaaaag 6180 gcgacagact ggaagaagga atttcaaaaa ccgccggggc tcatggcatt ccgcatacct 6240 ttaaccgtgc aggttcgatg atcggtttct tctttacaaa cgaaccagtc atcaattatg 6300 aaacagcgaa atcatctgat ttgaagctgt tcgcaagcta ttataaaggg atggcaaatg 6360 aaggggtatt ccttccgcca tcacaattcg aaggtctttt cctctcaacg gcccatactg 6420 atgaagatat tgaaaacaca atccaggcag ctgagaaagt atttgctgag atcagccgca 6480 gataagagtg aaaaccggta tcaaggactc cttgtgccgg ttttttcgtg ctctcccact 6540 catattttct ccagttcata catacacctt tagtgacatc taattttaag gattagatga 6600 aaggaggata tgaacttgcc gcaaaatcat cgat 6634 // |
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