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EBI DbfetchID M57629; SV 1; linear; genomic DNA; STD; PRO; 6959 BP. XX AC M57629; XX DT 02-MAY-1991 (Rel. 28, Created) DT 17-APR-2005 (Rel. 83, Last updated, Version 6) XX DE Pseudomonas sp. regulatory protein (tcbR), catechol 1,2-dioxygenase II DE (tcbC), cycloisomerase II (tcbD), ORF, hydrolase II (tcbE), and DE maleylacetate reductase (tcbF) genes, complete cds. XX KW . XX OS Pseudomonas sp. OC Bacteria; Proteobacteria. XX RN [1] RP 1-6959 RX PUBMED; 2013566. RA van der Meer J.R., Eggen R.I.L., Zehnder A.J.B., de Vos W.M.; RT "Sequence analysis of the Pseudomonas sp. strain P51 tcb gene cluster, RT which encodes metabolism of chlorinated catechols: evidence for RT specialization of catechol 1,2-dioxygenases for chlorinated substrates"; RL J. Bacteriol. 173(8):2425-2434(1991). XX RN [2] RP 1-6959 RX PUBMED; 8550433. RA Muller D., Schlomann M., Reineke W.; RT "Maleylacetate reductases in chloroaromatic-degrading bacteria using the RT modified ortho pathway: comparison of catalytic properties"; RL J. Bacteriol. 178(1):298-300(1996). XX RN [3] RP 1-6959 RA Van der Meer J.R.; RT ; RL Submitted (06-NOV-1997) to the EMBL/GenBank/DDBJ databases. RL Microbiology, EAWAG, Ueberlandstrasse 133, Duebendorf 8600, Switzerland XX RN [4] RC Sequence update by submitter RP 1-6959 RA Van der Meer J.R.; RT ; RL Submitted (09-FEB-1999) to the EMBL/GenBank/DDBJ databases. RL Microbiology, EAWAG, Ueberlandstrasse 133, Duebendorf 8600, Switzerland XX CC On Feb 9, 1999 this sequence version replaced gi:2587066. XX FH Key Location/Qualifiers FH FT source 1..6959 FT /organism="Pseudomonas sp." FT /strain="P51" FT /mol_type="genomic DNA" FT /db_xref="taxon:306" FT CDS complement(637..1521) FT /codon_start=1 FT /transl_table=11 FT /gene="tcbR" FT /product="regulatory protein" FT /db_xref="GOA:P27102" FT /db_xref="InterPro:IPR000847" FT /db_xref="InterPro:IPR005119" FT /db_xref="InterPro:IPR011991" FT /db_xref="UniProtKB/Swiss-Prot:P27102" FT /protein_id="AAD13624.1" FT /translation="MEFRQLKYFIAVAEAGNMAAAAKRLHVSQPPITRQMQALEADLGV FT VLLERSHRGIELTAAGHAFLEDARRILELAGRSGDRSRAAARGDVGELSVAYFGTPIYR FT SLPLLLRAFLTSTPTATVSLTHMTKDEQVEGLLAGTIHVGFSRFFPRHPGIEIVNIAQE FT DLYLAVHRSQSGKFGKTCKLADLRAVELTLFPRGGRPSFADEVIGLFKHAGIEPRIARV FT VEDATAALALTMAGAASSIVPASVAAIRWPDIAFARIVGTRVKVPISCTFRKEKQPPIL FT ARFVEHVRRSAKD" FT CDS 1671..2426 FT /codon_start=1 FT /transl_table=11 FT /gene="tcbC" FT /product="catechol 1,2-dioxygenase II" FT /EC_number="1.13.11.1" FT /db_xref="GOA:P27098" FT /db_xref="InterPro:IPR000627" FT /db_xref="InterPro:IPR007535" FT /db_xref="InterPro:IPR012817" FT /db_xref="InterPro:IPR015889" FT /db_xref="UniProtKB/Swiss-Prot:P27098" FT /protein_id="AAD13625.1" FT /translation="MNERVKQVASALVDAIQKTLTEQRVTEEEWRAGVGYMMKLAEAKE FT VAVLLDAFFNHTIVDLKAQATRGSRPAMQGPYFLEGAPVVAGALKTYEDDSHHPLVIRG FT AVRTDDGAPAAGAVIDVWHSTPDGKYSGIHDQIPTDMYRGKVVADAQGKYAVRTTMPAP FT YQIPNKGPTGVLLEMMGSHTWRPAHVHFKVRKDGFAPLTTQYYFEGGDWVDSDCCKGVA FT PDLVMPTKTEGGAQVMDIDFVIERAREHV" FT CDS 2423..3535 FT /codon_start=1 FT /transl_table=11 FT /gene="tcbD" FT /product="cycloisomerase II" FT /EC_number="5.5.1.1" FT /db_xref="GOA:P27099" FT /db_xref="InterPro:IPR001354" FT /db_xref="InterPro:IPR013341" FT /db_xref="InterPro:IPR013342" FT /db_xref="InterPro:IPR013370" FT /db_xref="InterPro:IPR018110" FT /db_xref="PDB:1NU5" FT /db_xref="UniProtKB/Swiss-Prot:P27099" FT /protein_id="AAD13626.1" FT /translation="MKIEAISTTIVDVPTRRPLQMSFTTVHKQSYVIVQVKAGGLVGIG FT EGGSVGGPTWGSESAETIKVIIDNYLAPLLVGKDASNLSQARVLMDRAVTGNLSAKAAI FT DIALHDLKARALNLSIADLIGGTMRTSIPIAWTLASGDTARDIDSALEMIETRRHNRFK FT VKLGARTPAQDLEHIRSIVKAVGDRASVRVDVNQGWDEQTASIWIPRLEEAGVELVEQP FT VPRANFGALRRLTEQNGVAILADESLSSLSSAFELARDHAVDAFSLKLCNMGGIANTLK FT VAAVAEAAGISSYGGTMLDSTVGTAAALHVYATLPSLPYGCELIGPWVLGDRLTQQDLE FT IKDFEVHLPLGSGLGVDLDHDKVRHYTRAA" FT CDS 3537..4547 FT /codon_start=1 FT /transl_table=11 FT /note="ORF" FT /db_xref="GOA:P27103" FT /db_xref="InterPro:IPR005064" FT /db_xref="UniProtKB/Swiss-Prot:P27103" FT /protein_id="AAD13627.1" FT /translation="MHSSKCPDLANIGRRRVLAGIALAMTTSSTRAQRLDAFPSRPITW FT VVPFPPGGAVDAIARIISRKMSESIGQQVVVDNRAGSGGIIGSDLVAKAPSDGHTILIN FT STGLVVDRFFYPRVPYQSDRDFVPVVLAATLPSVLVVPADSRFRDITQLLKAARENPGK FT LSFASAGLGTSIHLASALLAARANVELLHVPYRGSSPAVSDLVAGRVDMMIDSVTSQRQ FT NILAKRVRALGVTSLDRHPQLPEVPTIAEAAGLPGFEVLTWCGVFAPKGTPRSVVERLN FT AEINKAIAAPDVVEALKQLGIKTAGGAPEVLADLFKSETERWQKLIVEYRLNANQ" FT CDS 4569..5285 FT /codon_start=1 FT /transl_table=11 FT /gene="tcbE" FT /product="hydrolase II" FT /db_xref="GOA:P27100" FT /db_xref="InterPro:IPR002925" FT /db_xref="UniProtKB/Swiss-Prot:P27100" FT /protein_id="AAD13628.1" FT /translation="MLTEGLSIDAKGGGRFGAHLQLPARGRGPVVMVAQEIFGVNPFMT FT EVLAWLASEGFVGLCPDLYWRHGPGIEFDPNDEVQRARALGMFRDYKLEDGVADLRATV FT AYAASQPFCDGGVAVIGYCLGGALAYEVAAEGFAQCCVGYYGVGFEKRLERARLVKTPS FT MFHMGTNDHFVTAEARQLITNAFEANPAIALHWYDAGHSFARASSPNFSPEATRTANAR FT TLEMLKRMKPIGTIGQ" FT CDS 5282..6340 FT /codon_start=1 FT /transl_table=11 FT /gene="tcbF" FT /product="maleylacetate reductase" FT /db_xref="GOA:P27101" FT /db_xref="InterPro:IPR001670" FT /db_xref="UniProtKB/Swiss-Prot:P27101" FT /protein_id="AAD13629.1" FT /translation="MNFIHDPLTPRVLFGAGRLQSLGEELKLLGIRRVLVISTPEQREL FT ANQVAALIPGSVAGFFDRATMHVPSQIVDQAASVARELGVDSYVAPGGGSTIGLAKMLA FT LHSSLPIVAIPTTYAGSEMTSIYGVTENELKKTGRDRRVLARTVIYDPELTFGLPTGIS FT VTSGLNAIAHAVEGLYAPEVNPILAIMAQQGIAALAKSIPTIRSAPTDLEARSQAQYGA FT WLCGSVLGNVSMALHHKLCHTLGGTFNLPHAETHTVVLPHALAYNTPAIPRANAWLQEA FT LATREPAQALFDLAKSNGAPVSLQSIGMKEADLDRACELVMSAQYPNPRPLEKHAIANL FT LRRAYLGEPPQP" XX SQ Sequence 6959 BP; 1341 A; 2155 C; 2202 G; 1261 T; 0 other; gagctcgcgc acgtccgagc ggatggccga tctgatggtt gacctgatga ttcgttatct 60 tccggccgaa tcaggtcgac gttgcttggg cgccgtgccg ttccatgtgc aaaaagcgga 120 agccttcatg cgcgagaact ttcgggaagc gattacgctg gccgaggttg cgtcagccgc 180 cggagtaact aagcgctgcc tttgtataaa tttcaggcga tttcgaaata cgagtcctat 240 ggcgtacctg cgcctccacc gattgacgca ggctagaaag atgctcgatc gacgcaagct 300 cgcgcttccg tcacggaggt ggctggagaa tgcgcactgc atcacctggg gcgtttctct 360 caagagtacc gactcgagtt tggggaacta ccctcgcaga ccatcgagcg agtcaatcga 420 gcccacctca agtgatccga taagcttgct tgacggtagc tacgaatgct tgccatcaga 480 actgcaacct ttctgactct tttgcatctg tgacggcggg ctgggcgatg aaggctgggc 540 tacgaactcg tagcttgcga ccgcaacgcg atccctgtcc agcgtgacga tcggaccttg 600 cgaactcaaa cgtggttgag cgaataacca tttgcctcag tccttcgcgg atcgccgcac 660 gtgttccacg aaccttgcca ggatgggcgg ctgtttctcc ttgcggaagg tgcagctgat 720 gggcaccttg acccgtgtcc cgacgatccg cgcgaacgcg atatccggcc aacggatcgc 780 tgcgacagac gcagggacga tgctcgacgc cgcaccggcc atggtcaggg cgagcgcggc 840 cgtggcgtcc tccacgactc gggcgatgcg gggctcgata cccgcgtgct tgaacaggcc 900 gatcacctca tcggcgaagc tcggccggcc gccgcgcgga aacagtgtga gttccaccgc 960 gcgcaggtcc gcgagcttgc aggtcttgcc gaacttcccc gactgggagc ggtgcaccgc 1020 gaggtagaga tcctcttgcg cgatgttgac gatctcgatc ccgggatgcc ggggaaagaa 1080 gcggctgaag cccacgtgga tggtccccgc gagcaggccc tccacctgct cgtccttggt 1140 catgtgcgtg agggataccg tcgccgtagg cgtggacgta aggaaagcgc gcagcaaaag 1200 gggcaggctg cggtagatgg gcgttccgaa atacgccacg ctcaactcgc ccacgtcgcc 1260 ccgcgcggcg gcgcgggagc gatcacccga acggcctgcc aactccagga tgcggcgcgc 1320 atcctccagg aaagcgtgac ctgcggcggt gagttcgatc ccgcggtggc ttcgctccag 1380 aagcacgacg cccaagtccg cttccagggc ttgcatctgc cgcgtgatgg ggggctgtga 1440 gacgtgcagg cgcttggctg ctgcagccat gttccctgct tccgcgacgg cgatgaaata 1500 cttgagctgc cggaattcca tggcgcgctt tccgagttgg tgatgtgcct atattacgca 1560 aaccgtaacg atggctgact aatttggtat tggacggcat ggccggcgct gcctagcatt 1620 cactcgtccc gcgtcaatcc ggaccgggca aaccgacaaa ggagaccggg atgaacgaac 1680 gagtgaagca ggttgcgtcc gcattggtgg atgcgatcca gaagaccctc acggagcaac 1740 gcgtgacgga agaagagtgg cgcgcgggcg tcggctacat gatgaaactg gccgaggcca 1800 aggaagtggc tgtcctgctg gacgcgttct tcaaccacac gatcgtcgac ctcaaagccc 1860 aggccacgcg cggatccagg cccgcgatgc agggcccgta tttcctggaa ggcgccccgg 1920 tggtcgccgg cgccctgaag acctacgagg atgactcgca tcacccgctc gtgatccgcg 1980 gcgccgtgcg cacggacgat ggcgcgccag ccgcgggagc cgtgatcgac gtgtggcact 2040 ccacgcccga cggcaagtac agcggcattc atgaccagat cccgaccgac atgtaccgcg 2100 gcaaggtcgt ggccgatgcg cagggcaagt acgccgtgcg caccaccatg cccgccccgt 2160 accagatccc gaacaaggga ccgaccggcg tgctgcttga aatgatggga agccatacgt 2220 ggcgtcccgc gcacgtgcac ttcaaggtgc gcaaggacgg gttcgcgccg ctgaccacgc 2280 agtactactt cgaaggcggc gactgggtcg acagcgactg ctgcaagggt gtcgcaccgg 2340 acctggtcat gccgacaaag accgagggcg gcgcgcaggt gatggacatc gacttcgtca 2400 tcgagcgcgc gcgggagcac gtatgaaaat cgaggcgatt agcacgacga tcgtcgatgt 2460 cccgacacgt cgcccgttgc agatgtcgtt caccacggtg cacaagcaga gctatgtgat 2520 cgtccaggtg aaggccgggg gcctcgtggg gatcggtgaa ggcggtagtg tcggcgggcc 2580 gacctggggc tccgaatcgg ccgagaccat caaggtcatc atcgacaact accttgcgcc 2640 actcctggtc ggcaaggacg cgtccaacct gagccaggca agggttctca tggatcgcgc 2700 ggtcacgggc aacctgtcgg cgaaggccgc catcgacatc gcgctgcacg acctgaaagc 2760 gcgcgcgctg aacctatcga tcgccgactt gatcggcggg acgatgcgga ccagcatccc 2820 gatcgcatgg acacttgcga gcggcgacac cgcccgggac atcgactcgg cgctcgagat 2880 gatcgagacg cgacggcaca atcgcttcaa ggtgaagctc ggcgcgcgca cgccggctca 2940 ggatctggag cacatccgca gcatcgtgaa ggccgtgggc gacagggctt ccgtgcgtgt 3000 cgacgtaaac cagggatggg acgagcagac cgcttcgatc tggattccgc ggcttgaaga 3060 agcgggcgtg gaactggtcg agcagcccgt gcctcgcgcg aatttcgggg cccttcggcg 3120 tttgaccgaa caaaacgggg tggcgatcct tgccgatgag agcctcagct cgctgtcgtc 3180 ggcattcgag ctggcccgcg accacgcggt ggacgcgttc tcgctgaagc tttgcaacat 3240 gggcggcatc gcgaacacgc taaaggtcgc ggcggtcgcg gaagcagcgg gcatttcctc 3300 ctatggcgga acgatgctgg actccacggt cggcacagca gctgcactgc acgtgtacgc 3360 gacattgcct tccctgccct acggatgcga gttgatcggc ccttgggttc ttggtgaccg 3420 cctgacccag caggacctcg agatcaagga cttcgaagtg cacctgccgc tgggaagcgg 3480 gcttggcgtg gacctagacc acgacaaggt ccgtcactac acacgggcgg cctgacatgc 3540 actcctcgaa atgccctgac ctcgcgaaca tcggccgccg tcgcgtcctt gcgggcatcg 3600 cgctcgcaat gacgacaagt tcaaccaggg cacaacggct tgacgccttt ccttcgcgtc 3660 ccatcacatg ggtagttccc ttcccgccgg gcggagccgt cgatgcgatc gcgcgcatca 3720 tttcgcgaaa gatgtcagag agcatcgggc agcaggttgt ggtggataac cgcgcgggtt 3780 cgggcggcat catcggcagc gacctcgtcg cgaaggcccc gtctgacgga cacaccattc 3840 tgatcaactc cacgggactg gtggtggacc ggttcttcta tcctcgcgta ccgtaccagt 3900 ccgatcgcga cttcgttccg gtggttctcg cggcgacgct ccccagcgtt ctggtcgtgc 3960 cggcggattc gcggtttcgt gacatcacgc agctcctgaa agcggcgcgc gaaaaccccg 4020 gcaagttgtc ctttgcttcc gcgggattgg gcacgtctat ccaccttgcc tccgcgctcc 4080 tcgcggccag ggcgaacgtg gagctgctcc atgtccccta ccggggcagc agtccagccg 4140 tttcggacct ggtcgcgggc cgcgtcgaca tgatgatcga ctcagtgaca tcgcagcgcc 4200 agaacatcct cgccaagcgc gttcgcgcac tcggggtgac gagcctggat cgccatccgc 4260 aactgcccga agtgccgacc atcgccgagg cggcgggcct tcccgggttt gaagtgctca 4320 catggtgcgg ggtgttcgca cccaaaggca cgcctcgctc cgtcgtggag cggcttaacg 4380 ccgaaatcaa caaggcgatt gctgcgccgg acgtcgtcga agcacttaag cagcttggga 4440 tcaagactgc cggcggcgca ccggaagtcc ttgcggacct attcaagtcc gagacggagc 4500 ggtggcaaaa gctcatcgtc gaataccgcc tcaatgcaaa ccagtgaagt tgaaagagag 4560 acaagcacat gctgactgaa ggcttatcta tcgatgcgaa aggaggcggc cgcttcggcg 4620 cgcacctgca attgccggca cgcgggcgcg gcccggtggt gatggttgcg caggagattt 4680 ttggggtcaa cccattcatg accgaagtct tggcgtggtt ggcatcagaa ggttttgtgg 4740 gcctctgtcc tgatctgtat tggcgccacg gaccaggcat cgagttcgat ccgaacgacg 4800 aagtacaacg tgcgcgcgcg ctcgggatgt tccgcgacta caagctggaa gatggggtgg 4860 cggatttgcg ggccacagtc gcctacgcgg cttctcagcc gttttgcgac ggcggggtcg 4920 cggtgatcgg ctattgcctg ggcggagcgc tggcctacga agttgccgca gagggattcg 4980 cgcaatgctg tgtcggctac tacggcgtcg gcttcgagaa gcgtctcgaa cgcgcccgac 5040 ttgtgaagac accatcaatg ttccacatgg gcacgaacga ccatttcgtg accgcggagg 5100 ccagacaact catcacgaat gcctttgaag cgaatcccgc gattgcgctg cattggtacg 5160 acgccgggca ctcgttcgcg cgggcttcca gcccgaactt ctcaccggag gccacgagga 5220 cggcgaatgc acgcacgctg gaaatgctga agcgcatgaa gcccatcggg acaattggcc 5280 aatgaacttc atccacgacc cactcacgcc tcgcgtcttg ttcggcgcgg ggcgcctgca 5340 gtctctgggc gaagaactga agctgctcgg gatacggcgc gtgctggtta tctccacccc 5400 ggagcagcgt gaactcgcca accaggtcgc ggccctcatt cccggcagcg tcgccggatt 5460 cttcgaccgc gcgacgatgc acgtgccgtc gcaaatcgtc gatcaggcgg cttctgtcgc 5520 gcgagaactc ggcgttgaca gctatgtggc ccctggcgga ggttcaacca tcggcctcgc 5580 gaagatgctc gcgctccact cgtcattgcc aatcgtggcc atccccacga cgtatgccgg 5640 aagcgagatg acatccatct atggcgtcac cgaaaacgaa ttgaagaaga ccggtcggga 5700 taggcgcgtc ctcgcgcgca cggtcatcta cgacccggag ctgacattcg gcctgcccac 5760 gggcatcagc gtgacaagcg gtttgaacgc gatcgcgcac gcggttgagg gcctttacgc 5820 gccggaggtg aacccgatcc tcgcgatcat ggcccagcaa ggaatcgcgg cgttggccaa 5880 atcaattccc acgatccgtt cggctccgac cgaccttgag gcccgttcgc aagcgcaata 5940 cggtgcatgg ctgtgcggaa gtgtgctcgg caatgtcagc atggccctgc accacaagct 6000 gtgccatacg ttggggggaa cattcaatct gccgcacgct gaaacgcaca ctgtcgtgct 6060 gccgcacgcg ctcgcctaca acacgccggc catcccgcgt gcgaatgcat ggctgcagga 6120 ggcactcgcc actcgcgaac cggcgcaggc actgtttgat ctcgcgaagt cgaacggcgc 6180 gccggtttca ctgcaatcga tcgggatgaa ggaagcggac ttggaccgcg cctgcgagct 6240 ggtgatgtcc gcgcaatacc ccaatccgcg accgctcgaa aagcatgcga tcgcgaatct 6300 gctgcgcagg gcctatctag gggagccacc gcaaccctga ccttgccgca ggccaccgtt 6360 cggtggcata ttgattgctt gggaaaacaa tgcgcccccg cctatgtagc tcgcggcgca 6420 aatggagact gccatgcacc aacaaggttc atctttcgac ctgtcgaatc tgcagcgcaa 6480 ctgcattttc cgatgcgaat cgccgatgga gtcgcacgag aagatcgcgc acgaattggc 6540 cgaccacgat ttgcgctggc ggcggggcgc cgttgacacc ggcatgtaca aggtcggggt 6600 tcacgccttg gaaatgttca tgctgcacta tggcgccgaa gtggaggtgc ggccccgcgt 6660 tcccgaaggg ttcgcgctgg tccatctctc gttgaaaggc gcgatcgaca tcatctccga 6720 cgagcagcgc atcacggttt cgcccggcga agtcgcgtat ctcgcgccgc gcaagaattt 6780 ttcggatgtt gtggcagcgc ggtgcacagc agctgattct gaaggtgccc gacaccttgc 6840 tgcgttccat tgtggccgag gcgggcgcgt gcctggactt gccgtcaacc atgagattga 6900 cgccggcagc cgccaggcaa tggcacttgt tcatgcaatc gctgctcaac atcggtacc 6959 // |
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