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EBI DbfetchID M57500; SV 1; linear; genomic DNA; STD; UNC; 6164 BP. XX AC M57500; XX DT 01-MAY-1991 (Rel. 28, Created) DT 15-JAN-2007 (Rel. 90, Last updated, Version 10) XX DE Plasmid pEST1226 putative transposase (tnpA), catechol 1,2-dioxygenase DE (pheB), phenol monooxygenase (pheA), and putative transposase (tnpA) genes, DE complete cds. XX KW . XX OS Plasmid pEST1226 OC other sequences; plasmids. OG Plasmid pEST1226 XX RN [1] RP 1-6164 RX DOI; 10.1016/0378-1119(91)90098-V RX PUBMED; 2013408. RA Kivisaar M., Kasak L., Nurk A.; RT "Sequence of the plasmid-encoded catechol 1,2-dioxygenase-expressing gene, RT pheB, of phenol-degrading Pseudomonas sp. strain EST1001"; RL Gene 98(1):15-20(1991). XX RN [2] RP 2527-4953 RX DOI; 10.1016/0378-1119(91)90531-F RX PUBMED; 1650730. RA Nurk A., Kasak L., Kivisaar M.; RT "Sequence of the gene (pheA) encoding phenol monooxygenase from Pseudomonas RT sp. EST1001: expression in Escherichia coli and Pseudomonas putida"; RL Gene 102(1):13-18(1991). XX RN [3] RP 1-1557 RX PUBMED; 8253692. RA Kasak L., Horak R., Nurk A., Talvik K., Kivisaar M.; RT "Regulation of the catechol 1,2-dioxygenase- and phenol RT monooxygenase-encoding pheBA operon in Pseudomonas putida PaW85"; RL J. Bacteriol. 175(24):8038-8042(1993). XX RN [4] RP 1-1557, 4954-6164 RX PUBMED; 9765560. RA Kallastu A., Horak R., Kivisaar M.; RT "Identification and characterization of IS1411, a new insertion sequence RT which causes transcriptional activation of the phenol degradation genes in RT Pseudomonas putida"; RL J. Bacteriol. 180(20):5306-5312(1998). XX RN [5] RP 1-6164 RA Kivisaar M.; RT ; RL Submitted (26-APR-1993) to the EMBL/GenBank/DDBJ databases. RL Genetics, Institute of Molecular and Cell Biology, 23 Riia, Tartu EE2400, RL Estonia XX RN [6] RC Sequence update by submitter RP 1-6164 RA Kivisaar M.; RT ; RL Submitted (21-OCT-1998) to the EMBL/GenBank/DDBJ databases. RL Genetics, Institute of Molecular and Cell Biology, 23 Riia, Tartu EE2400, RL Estonia XX DR GOA; P31020. DR UniProtKB/Swiss-Prot; P31020; PHEA_PSEUE. XX CC On Oct 21, 1998 this sequence version replaced gi:145127. XX FH Key Location/Qualifiers FH FT source 1..6164 FT /organism="Plasmid pEST1226" FT /plasmid="pEST1226" FT /mol_type="genomic DNA" FT /db_xref="taxon:2553" FT gene 329..1363 FT /gene="tnpA" FT CDS 329..1363 FT /codon_start=1 FT /transl_table=11 FT /gene="tnpA" FT /product="putative transposase" FT /db_xref="GOA:Q52158" FT /db_xref="InterPro:IPR002559" FT /db_xref="UniProtKB/TrEMBL:Q52158" FT /protein_id="AAC64899.1" FT /translation="MTFADAEYAGKRKQTRKELFLIEMDRVVPWKGLIALIEPHYPKGE FT GGRPAYPLMAMLRVHLLQNWFGYSDPAMEEALYETTILRQFAGLNLERIPDETTILNFR FT RLLEKHELAAGILAVINGYLGDRGLSLRQGTIVDATLINAPSSTKNKDGKRDPEMHQTK FT KGNQYYFGMKAHIGADDESGLVHSVVGTAANVADVTQVDKLLHGDENVVCADAGYTGVE FT KRPEHEEREVIWQVRHAAAPTKNSISAACCTKPSARLKRPRLRCAPRSSIVPGNQAPVR FT LHQGALSRLGQKHGATGDTVRSVEPVDGAPTFTDECRRGAPVMREMATGVGSGWRKRKK FT YSSN" FT repeat_region 329..1363 FT /mobile_element="insertion sequence:IS1472" FT gene 1672..2580 FT /gene="pheB" FT CDS 1672..2580 FT /codon_start=1 FT /transl_table=11 FT /gene="pheB" FT /product="catechol 1,2-dioxygenase" FT /db_xref="GOA:P31019" FT /db_xref="HSSP:1DMH" FT /db_xref="InterPro:IPR000627" FT /db_xref="UniProtKB/Swiss-Prot:P31019" FT /protein_id="AAC64900.1" FT /translation="MTVKIYDTPEVQDFLKIVAGLDQEGGNDRGKQIIHRILSDLYRTI FT DDFDITAEQYWSAVSLLNALGQASQFGLLSPGLGFDHYMDMRMDAADAEAKRTGGTPRT FT IEGPLYVAGAPEAEGFARMDDDPDTDGETMWLHGQVRDTAGKPIPGAKVEIWHCNSKGG FT YSFFDKSQTPYNLRRTIIADNEGYYRARSVIPSGYGVPEGAPTDQVLKLLGRHGERPAH FT IHYFISAPGHQHLTTQINLAGDPYTYDDFAFATRQDLAAEGKRVENHPAAQQYGVEGTV FT TEVIFNIELSPTAEEELQARP" FT gene 2725..4557 FT /gene="pheA" FT CDS 2725..4557 FT /codon_start=1 FT /transl_table=11 FT /gene="pheA" FT /product="phenol monooxygenase" FT /db_xref="GOA:Q52159" FT /db_xref="InterPro:IPR003042" FT /db_xref="UniProtKB/TrEMBL:Q52159" FT /protein_id="AAC64901.1" FT /translation="MTTQRNDNLEQPGRSVIFDDGLSATDTPNETNVVETEVLIVGSGP FT AGSSAAMFLSTQGISNIMITKYRWTANTPRAHITNQRTMEILRDAGIEDQVLAEAVPHE FT LMGDTVYCESMAGEEIGRRPTWGTRPDRRADYELASPAMPCDIPQTLLEPIMLKNATMR FT GTQTQFSTEYLSHTQDDKGVSVQVLNRLTGQEYTIRAKYLIGADGARSKVAADIGGSMN FT ITFKADLSHWRPSALDPVLGLPPRIEYRWPRRWFDRMVRPWNEWLVVWGFDINQEPPKL FT NDDEAIQIVRNLVGIEDLDVEILGYSLWGNNDQYATHLQKGRVCCAGDAIHKHPPSHGL FT GSNTSIQDSYNLCWKLACVLKGQAGPELLETYSTERAPIAKQIVTRANGSSSEYKPIFD FT ALGVTDATTNDEFVEKLALRKENSPEGARRRAALRAALDNKDYEFNAQGTEIGQFYDSS FT AVITDGQKRPAMTEDPMLHHQKSTFPGLRLPHAWLGDAKEKYSTHDIAEGTRFTIFTGI FT TGQAWADAAVRVAERLGIDLKAVVIGEGQPVQDLYGDWLRQREVDEDGVILVRPDKHIG FT WRAQSMVADPETALFDVLSALLHTKQTGSSHLRV" FT CDS 4639..5940 FT /codon_start=1 FT /transl_table=11 FT /gene="tnpA" FT /product="putative transposase" FT /note="ORF2; similar to IS1096 transposase sequence in FT GenBank Accession Number M76495" FT /db_xref="GOA:Q52160" FT /db_xref="InterPro:IPR002560" FT /db_xref="UniProtKB/TrEMBL:Q52160" FT /protein_id="AAC64902.1" FT /translation="MNNLTFSSPDLSSFCQLNNLGLTATGQHLCAERAVIECRLTKAPE FT PCPKCGAAGVSRGTVDRHLAHTPYGQRPTRLLLRIRRWRCACGCFWHEDTNSAAPPRSK FT LSYGAIRWALAAIVLDHLSVSRVASQLDVAWHTANNAIINEGRRLLFNDSTRFDGVTVL FT GVDEHVWRHTRCGDKYVTIVVDLTPVRNKNGPARLLDVLEGRSKQAFKQWLQSRPKSWR FT DQIESIAMDGFTGFKSAAQEALPQAQTVLDPFHVVRWASNMLDECRRRVQHDILGRRGR FT KNDPLYKSRRTLLTRISYLSDANKKQLFQLFADEHHLEVDCTWSMYQRVVSAYNEPDRK FT RGKKLMEEVINIITVSDLPKALIEVKGLGETLKKYAESILAYFDRPGTSNGPTEAINGR FT LEHLRGTALGFRNLTNYIARCLLKSGGFRNQLHP" FT repeat_region 4639..5940 FT /mobile_element="insertion sequence:IS1411" XX SQ Sequence 6164 BP; 1578 A; 1614 C; 1615 G; 1357 T; 0 other; aactccactc atacctccta ggtatcgctg aggacttaat cagtattgga ttgcattcct 60 aaagaagctg tagctttgag ttaacaacaa aaacacaaca ctaaaagttg ggaggcagca 120 gatgcccgta atcatcgagt ccatagaagc tagcctgctc agtattccga ccctagatct 180 gaatacactt tgcattatag ccacggacgc acagagtgcg tttgtcatgg atagaaaaaa 240 cagcagtgtt cttaaggaaa ctctgaaaaa gactttctga tttggcaaaa taccgcgacc 300 ccacccaccg agtttcccga tgaagcagat gaccttcgcc gacgccgagt acgctggcaa 360 gcgcaagcaa acccgcaagg agttgttcct gatcgagatg gatcgggtgg tgccgtggaa 420 gggcttgatt gctttgatcg agccacatta tccgaaaggt gaaggtggcc gtccggccta 480 cccgttgatg gcgatgctgc gtgtgcatct gctgcagaac tggttcggct acagcgatcc 540 agcgatggag gaagcgctgt acgaaaccac gatcctgcgc cagtttgccg ggctgaacct 600 ggagcgcatc cccgacgaaa ccaccattct caacttccgc cgcttgctgg agaaacacga 660 gctggcggcc ggcatcctcg ctgtcatcaa tggctatctg ggcgaccgcg gcctgtcgct 720 gcgccagggc accatcgtcg atgcaacgct gatcaatgcg cccagttcga ccaagaacaa 780 ggacggcaag cgcgacccgg aaatgcacca gaccaagaag ggaaaccagt attattttgg 840 catgaaggcc cacatcggcg ccgatgacga atcgggtctg gtgcacagcg tagtgggcac 900 ggcggccaat gtggcggatg tcacccaggt ggacaaattg ctgcatggcg acgaaaacgt 960 ggtctgcgcc gatgcaggct acaccggtgt cgaaaagcgg cccgagcatg aagaacgtga 1020 agttatctgg caggtgcggc acgccgcagc acctacaaaa aactcgataa gcgcagcgtg 1080 ctgtacaaag ccaagcgcaa gattgaaaag gccaaggctc aggtgcgcgc caaggtcgag 1140 catcgttccg ggtaatcaag cgccagttcg gttacaccaa ggtgcgcttt cgcggcttgg 1200 ccaaaaacac ggcgcaactg gtgacactgt tcgctctgtc gaacctgtgg atggcgcgcc 1260 gacatttact gacgaatgca ggagaggtgc gcctgtaatg cgggaaatgg ccaccggagt 1320 ggggtccggg tggcgcaaac gtaagaaata ctcgtcgaat tgattgtttt ttgatcattt 1380 ggcgagttta aaagttgcgc taggtggtat gccggggaaa tacatggcta cttcagacca 1440 tccttaaggc tcaagccgag tctacgacta tcgataccct aaaggtaagc ttgaacaata 1500 gcacccatgg caactgcttg acgaagtctg cccgtacgac tgccaagcat acttccattg 1560 aagcgccgtt tacggtcgac ttgcaaggac gatagccaaa ggactcacct ccattgccaa 1620 tgctcgatgg gaatacatca caacgacaat aaaaatgatg agagagtaat tatgactgtt 1680 aaaatttatg acacccctga agtgcaagat tttctaaaaa tcgttgctgg tctcgaccaa 1740 gagggcggta acgatcgtgg caagcagatc attcaccgca ttctatctga cttgtatcgc 1800 accattgacg attttgatat cactgctgag caatactggt ctgccgtcag tctgcttaac 1860 gctttgggac aagcttcgca gtttggcttg ctatcacccg gcctaggttt tgatcattat 1920 atggacatgc gcatggatgc cgccgatgca gaagccaagc gcaccggcgg caccccgcgt 1980 accatcgaag gaccactgta tgttgctggt gctcctgagg cagagggttt tgcgcgcatg 2040 gatgatgatc cagatacgga tggcgaaacc atgtggttgc atggtcaagt gcgtgatacg 2100 gctgggaaac caatccccgg cgccaaagta gagatttggc actgcaactc caaagggggt 2160 tactccttct ttgataagag ccaaacccct tacaatttgc gccgcaccat tatcgctgac 2220 aatgaaggct actaccgtgc ccgcagtgtt attccatcag gctacggtgt acctgaaggc 2280 gctcctacgg atcaagtact gaaattactc ggtcgccatg gcgaacgccc agcgcatatt 2340 cactacttta tttccgcgcc aggtcatcag catctaacca cgcaaattaa cttggctggc 2400 gacccctaca cctatgatga ctttgctttt gcaacccgtc aagacttagc ggcggaaggc 2460 aagcgcgtag aaaaccaccc agccgcgcaa caatacggcg ttgaaggcac tgtgaccgaa 2520 gtgatattca atattgaact gtcgcccact gcagaagagg agctgcaagc gcgtccataa 2580 gcgtccacgt gccatggaaa gcgagtcaca agaaagtcag ctagccggca ctgccaaggt 2640 ctaatacata caaataaact gttgcacacc ggggagcgct gattaaggcg ctcccgtaag 2700 acaataagaa ttgaggtttc gaccatgact acacagcgta atgataatct tgagcagccg 2760 ggccgtagcg tcatttttga tgatgggctg agcgcaactg ataccccaaa tgagaccaac 2820 gtagttgaaa ctgaggtgtt aattgtcggt tcaggccctg ctggcagctc cgcagcaatg 2880 ttcctgtcga cccagggcat tagcaacatt atgatcacca aataccgttg gactgcgaat 2940 accccccgtg cgcatatcac taaccagcgc accatggaaa ttttacgcga cgctggtatt 3000 gaggatcagg ttttagcaga agcagtcccc catgaactta tgggtgacac agtctattgt 3060 gagtcaatgg ccggcgaaga aattggccgc cggccaactt ggggcacacg acctgaccgc 3120 cgcgctgact atgagctggc atctccagcg atgccttgcg atatcccgca aaccttgctt 3180 gagcccatta tgctcaaaaa tgccaccatg cgtggcacgc aaacacagtt ctccactgag 3240 tatttaagcc acacccaaga cgataagggt gtcagcgtgc aagtactcaa ccgtctgacc 3300 ggtcaagaat ataccattcg cgccaaatac ctgattggtg ctgatggtgc gcgctccaaa 3360 gtggctgcgg atatcggcgg ctcgatgaat atcaccttta aagcagactt gtcccactgg 3420 cgcccatcgg ccctcgatcc tgtattgggt cttccgccca ggatcgaata tcggtggcct 3480 cggcgctggt ttgatcgcat ggtgcggcca tggaatgaat ggctggtggt ctggggtttt 3540 gatatcaatc aagagccacc caagctcaat gacgatgaag ctattcaaat cgtgcgtaat 3600 ctagtgggta tcgaggatct tgatgtggaa atccttggct actcactctg gggcaataat 3660 gaccagtacg ccacgcatct acagaaaggc cgcgtatgct gtgccggtga tgcaatccat 3720 aagcatccgc ccagtcacgg cctgggttct aatacgtcaa tccaagactc ctacaacctg 3780 tgctggaagt tggcctgtgt actcaaaggg caggcggggc ctgaactgtt agaaacctat 3840 tccaccgagc gtgcacccat cgccaagcag attgtgacgc gtgccaacgg ctcgagcagt 3900 gaatataagc cgatttttga cgctttaggc gttaccgatg cgacaaccaa cgatgagttt 3960 gtagaaaagc ttgccttgcg taaggaaaat tcgcctgaag gtgctcgccg tcgagcagca 4020 ttgcgtgcgg cgctggacaa taaggattat gagtttaacg cccaaggcac tgaaattggt 4080 cagttctacg actcatcagc agtgattact gatggtcaaa aacgcccagc aatgaccgag 4140 gatcctatgc tacaccacca gaaatcgacc tttcctggac tacgcctacc ccatgcatgg 4200 ctaggtgatg cgaaagagaa atactccacc catgatattg cggagggcac tcgcttcacg 4260 attttcactg gtatcaccgg tcaagcttgg gctgatgcag cagttcgcgt tgctgagcgt 4320 ttgggcatcg acttgaaggc cgtggtgatt ggtgaagggc agccggtaca agacctctat 4380 ggcgattggt tacgccagcg tgaagtggac gaggacggtg tgatcttggt gcgcccagat 4440 aaacatattg gttggcgtgc ccagagtatg gtcgcagatc cagagactgc attatttgat 4500 gtactctcag cgctgctgca taccaagcaa accggctctt cgcatttaag ggtgtagaaa 4560 aaatctgacc ggctggaaaa ggagtcgagg atcttagaaa atgtaagctc tcacaccaac 4620 aaaaactaag cccccgacgt gaacaatctt accttttcta gccctgatct ttccagcttt 4680 tgccaactga ataacctcgg cctgactgcc actggacaac atctttgtgc agagcgcgcc 4740 gtcatcgaat gtcgtttaac caaagcaccc gagccatgcc ccaagtgcgg ggctgctggt 4800 gtttcacgcg gtactgtcga taggcatctt gctcacacac cttacggaca acgaccaacc 4860 agattgctgc tgcgtatccg tcgctggcgt tgtgcttgcg gctgtttctg gcatgaagat 4920 acaaacagtg cagcacctcc acgttcaaag ctttcatatg gggcgatacg ctgggcatta 4980 gctgccatcg tgctggatca tctgtcggta tctcgtgttg cgagccagct tgatgttgca 5040 tggcacaccg ctaataatgc cattatcaac gaaggacggc gcctgctttt taatgactcg 5100 acacgttttg acggtgtgac cgtgctgggc gtggatgagc atgtttggcg acatacacgc 5160 tgtggtgaca agtacgtcac catcgtggtt gaccttacgc ccgtgcgtaa caaaaacggg 5220 ccggcccgct tgctcgatgt gctggaaggc cgctccaaac aagcctttaa gcaatggcta 5280 cagagtcgcc ccaaatcgtg gcgtgaccag atcgaaagca ttgccatgga cggttttacg 5340 gggtttaaat ctgcagcgca agaagccctg cctcaagccc aaaccgtgct ggatcctttc 5400 catgtcgtgc gctgggcaag caacatgctg gatgaatgcc gccggcgtgt gcaacacgac 5460 atcctgggcc gcagagggcg taaaaatgac ccgctctaca aaagccgtcg aacgctactg 5520 actcggatca gctacctgtc tgacgccaac aaaaagcaac tgttccagct gtttgcagat 5580 gagcaccacc tcgaagtgga ttgcacctgg agcatgtacc aacgggtggt cagcgcctac 5640 aacgagccgg atcgaaaacg cggtaaaaaa ctcatggaag aggtcataaa tatcattaca 5700 gttagcgact tgcccaaagc gctcatcgag gtgaaaggct tgggggaaac gctgaaaaaa 5760 tacgctgaga gcatccttgc ctacttcgac cggccaggaa ccagcaacgg tccaacagaa 5820 gctatcaacg ggcgacttga gcacttacga ggtaccgcct tgggctttag aaatttaacc 5880 aattacatag ccaggtgctt gctgaagtca ggagggttta gaaatcagct acacccttaa 5940 atgcgaagag cctgttatca aggcctgggc atttgtgcag tatacaaccc agttctcacc 6000 tccgaatgaa tcccagatcg catcaatttc atcgccagcg acctcggcta gatagcgatc 6060 aatttcgtct acctgagagt gatcatatgt tgcgcatatg agcttctcgt cagtcgtcaa 6120 aattccatat gatcggcttc ttctccaaga ctggagaata tagt 6164 // |
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