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EBI DbfetchID M35107; SV 1; linear; genomic DNA; STD; PRO; 5120 BP. XX AC M35107; XX DT 19-JUL-1990 (Rel. 24, Created) DT 17-APR-2005 (Rel. 83, Last updated, Version 6) XX DE C.acetobutylicum beta-D-galactosidase (cbgA) and beta-D-galactosidase DE regulatory protein (cbgR) genes, complete cds. XX KW beta-D-galactosidase; beta-D-galactosidase regulatory protein. XX OS Clostridium acetobutylicum OC Bacteria; Firmicutes; Clostridia; Clostridiales; Clostridiaceae; OC Clostridium. XX RN [1] RP 1-5120 RA Scott D.B., Hancock K.R., Pearce L., Maddox I.S.; RT ; RL Unpublished. XX RN [2] RP 1260-5120 RX PUBMED; 1850729. RA Hancock K.R., Rockman E., Young C.A., Pearce L., Maddox I.S., Scott D.B.; RT "Expression and nucleotide sequence of the Clostridium acetobutylicum RT beta-galactosidase gene cloned in Escherichia coli"; RL J. Bacteriol. 173(10):3084-3095(1991). XX CC Draft entry and computer-readable sequence for [1] kindly submitted CC by D.B.Scott, 11-JUN-1990. CC Author address:D.B.Scott: CC Molecular Genetics Unit CC Department of Microbiology and Genetics CC Massey University CC Palmerston North, New Zealand CC E-mail:D.B.Scott@massey.ac.nz. XX FH Key Location/Qualifiers FH FT source 1..5120 FT /organism="Clostridium acetobutylicum" FT /mol_type="genomic DNA" FT /db_xref="taxon:1488" FT CDS 1560..4253 FT /codon_start=1 FT /transl_table=11 FT /note="beta-D-galactosidase (cbgA)" FT /db_xref="GOA:P24131" FT /db_xref="HSSP:1JZ8" FT /db_xref="InterPro:IPR013781" FT /db_xref="UniProtKB/Swiss-Prot:P24131" FT /protein_id="AAA23216.1" FT /translation="MINNKPSLDWLENPEIFRVNRIDAHSDTWFYEKFEDVKLEDTMPL FT KQNLNGKWRFSYSENSSLRIKEFYKDEFDVSWIDYIEVPGHIQLQGYDKCQYINTMYPW FT EGHDELRPPHISKTYNPVGSYVTFFEVKDELKNKQTFISFQGVETAFYVWVNGEFVGYS FT EDTFTPSEFDITDYLREGENKLAVEVYKRSSASWIEDQDFWRFSGIFRDVYLYAVPETH FT VNDIFIKTDLYDDFKNAKLNAELKMIGNSETTVETYLEDKEGNKIAISEKIPFSDELTL FT YLDAQNINLWSAEEPNLYTLYILVNKKDGNLIEVVTQKIGFRHFEMKDKIMCLKWKRII FT FKGVNRHEFSARRGRSITKEDMLWDIKFLKQHNINAVRTSHYPNQSLWYRLCDEYGIYL FT IDETNLESHGSWQKMGQIEPSWNVPGSLPQWQAAVLDRASSMVERDKNHPSVLIWSCGN FT ESYAGEDIYQMSKYFRKKDPSRLVHYEGVTRCREFMTRRHESRMYAKAAEIEEYLNDNP FT KKPYISCEYMHSMGNSTGGMMKYTELEDKYLMYQGGFIWDYGDQALYRKLPDGKEVLAY FT GGDFTDRPTDYNFSGNGLIYADRTISPKAQEVKYLYQNVKLEPDEKGVTIKNQNLFVNT FT DKYDLYYIVERDGKLIKDGYLNVSVAPDEEKYIELPIGNYNFPEEIVLTTSLRLAQATL FT WAEKGYEIAFGQKVIKEKSDMNNHNSESKMKIIHGDVNIGVHGKDFKAIFSKQEGGIVS FT LRYNNKEFITRTPKTFYWRATTDNDRGNRHEFRCSQWLAATMGQKYVDFSVEEFDEKIT FT LYYTYQLPTVPSTNVKITYEVSGEGIIKVNVKYKGVSGLPELPVLGMDFKLLAEFNSFS FT WYGMGPEENYIDRCEGAKLGIYESTQ" FT CDS 4500..4805 FT /codon_start=1 FT /transl_table=11 FT /note="beta-D-galactosidase regulatory protein (cbgR)" FT /db_xref="UniProtKB/Swiss-Prot:P24132" FT /protein_id="AAA23217.1" FT /translation="MLGSTMIPKFCIDSSKDLEYSFIISKIILRTYGNYRYPKQNLDLC FT NNLRKDRYSVVSVTKNKLRLSNIISCTISACSRDKLVTSKNGTTYDEEMPATYSLM" XX SQ Sequence 5120 BP; 1921 A; 683 C; 876 G; 1640 T; 0 other; gaattccttt tcatatatat ctttaatatt tctactggaa tagaagaggt tgctcaatac 60 aaaaaatgct tctttaaaac tatttgaaac tacttctgaa atattttcta gcttactaaa 120 tagagaatta taatttttat catcaaaatt tagaattaca actatgattt cgttttcaat 180 attagcaatt tgtatattat aattgctatt taatccgtct aaagaaaatt ctttgccgat 240 ttctgaaatt gtaaaatcaa taatttcatg gcgtttgcta taattatcat atatttcttt 300 gcgtttaaac caaataagca aaatgattga aaagtaaata tgtatcaaag tagttaaagt 360 caggatcatg tcaaaacctg atataaggcg atttaaggcg ctattagtga gacttaaaga 420 gtttccttct aaagtatttc ttttcatttt tattgaaatc ttttttagag tacttaataa 480 ctcagaagga tttagagaag gttttaaaat ataatcaaca gcaccatttt gaaaagatga 540 tttaacatat tcaaaatcgc tataactact taagatgata attcttatct taggatattt 600 gtcctgcaca aatttagcta attcaacccc atttatttgg ggcattacaa catcagaaat 660 tataatgtca ggaatatcct tttttatcat ttccagagct tcttgaccat tagaagcctg 720 tcctataatt tgaaagcctt ctttttccca atcaatcata tgagttatgc cttgccgcat 780 aataaattca tcatcaacaa ctaaattttt actatattcg ttcaatagta tagcacccct 840 tattctaaaa ttaccacaac atagataaat attgcttaat actattatac cttatagatt 900 tattgtatgt atctgtatac gttacgttaa ttcatctaca aatttatatg agttttggtt 960 gcacttttag agaaaatctt tttgtctatg gtcttattgt cctataatgg tcaaatcatc 1020 tttaccaaag tctcttgatt taaagagata aaaacaccac tgatccatta ttcctcattt 1080 tggtaatgaa cctatgcggt tgaagatatt aatcagatgt ctaaatactt tagaaaaaaa 1140 gacctttact aatatcttca atatttacac ccctattcta aaattaccac aagatagata 1200 aatattgctt aatactgatt ataccttata gattaaaggt tttcaattaa acaataaatt 1260 actttagtaa agtttagtaa aatataattg attttttact aaaaagataa taaaatgaaa 1320 ctataaattt agttaatagc ataaatctaa catcagaaga taggataaat taaagaagta 1380 atgtaattga ttacgaaaca aaatctcata ttaatattag cccataattt ttttattctc 1440 atatatgttt aagtattaat taaatgtgac tttataaaaa ggttgcattt agttaatacg 1500 attaacaact ttaatttaaa aaagcaataa ctctacaaag tgaaagtgag ggggtaagta 1560 tgattaataa taaaccgtca ttagattggc tagaaaatcc ggaaatattt agagttaata 1620 gaatagatgc tcattctgat acttggtttt atgaaaaatt tgaggatgtt aaattagaag 1680 acaccatgcc tcttaagcaa aatttaaatg gaaaatggag attttcatat agtgaaaatt 1740 catcattaag aattaaagag ttttataagg atgagtttga cgtaagttgg attgattata 1800 ttgaagttcc aggtcatatt cagcttcaag gatatgataa atgtcaatat attaatacta 1860 tgtatccttg ggaaggtcac gatgaattaa gaccacctca tatttcaaaa acatataatc 1920 cggtgggaag ctatgtaaca ttttttgaag ttaaagatga actcaaaaat aagcagactt 1980 ttatttcttt tcaaggtgtt gaaacagcat tttacgtatg ggtaaatgga gaatttgtag 2040 gatatagcga agatacattt acaccatcag aatttgatat tactgattat ttaagagagg 2100 gagaaaataa acttgcagtt gaggtttata aaaggagtag cgcaagttgg atagaagatc 2160 aagatttctg gagattttca ggcatcttta gagatgtata tttatatgca gttccagaaa 2220 ctcatgtaaa tgatatattt ataaaaacag atttatatga cgatttcaaa aacgcaaagt 2280 taaatgctga acttaaaatg attggaaatt cagaaacaac agttgaaaca tatttagaag 2340 ataaagaagg aaataaaata gctatatctg aaaagattcc gttctctgat gagttgactt 2400 tatatttaga tgcgcaaaat ataaacctat ggagtgcaga agagcctaac ttatatacac 2460 tttatatttt agtgaataaa aaagatggta atttaattga ggttgtaact caaaagatag 2520 ggtttaggca ctttgaaatg aaggataaaa ttatgtgtct aaaatggaaa cgtattatct 2580 ttaaaggcgt aaaccgtcac gaatttagcg caagacgtgg acgctcaatt acgaaagagg 2640 acatgttgtg ggatattaag ttcttgaaac aacacaatat taatgctgtt agaacatcac 2700 attatccaaa tcaaagttta tggtacagac tttgcgatga atacgggatt tatttaatag 2760 atgaaacaaa tttagaaagc catggttcat ggcaaaagat ggggcagatt gaaccatcat 2820 ggaatgtgcc aggaagtctt ccacagtggc aggcagcagt tttagatcga gcatcatcaa 2880 tggttgaaag agataaaaat catccatctg tacttatttg gtcatgtggt aatgaatcct 2940 atgcgggtga agatatttat cagatgtcta aatactttag aaaaaaagat ccttcacgtt 3000 tagtgcacta tgaaggggta actagatgca gagaatttat gacacgacga catgaaagta 3060 gaatgtatgc aaaggcagca gaaatagaag aatatcttaa tgataatccg aagaaacctt 3120 atatcagctg cgaatacatg cactcaatgg gtaactcaac tggtggaatg atgaaataca 3180 cagaacttga agataaatat ttgatgtatc aaggtggatt catttgggat tacggcgatc 3240 aggcgttgta tagaaaactt ccagatggaa aagaagttct agcttatgga ggagacttta 3300 cagatcgtcc aacagactat aatttctctg gaaatggttt gatttatgca gatagaacta 3360 tatcacctaa agcacaggaa gttaagtatc tatatcaaaa cgtaaaatta gaaccagatg 3420 aaaaaggggt gactattaag aatcaaaatc tttttgttaa tactgataaa tatgatttat 3480 actatatcgt tgaaagagat ggaaaactaa taaaagatgg ttatctaaat gtatctgtag 3540 ctccagatga agaaaaatat atagaacttc caataggaaa ttacaatttt cctgaagaaa 3600 ttgtacttac aacctcatta agattagcac aagctacact ttgggcagaa aaaggatatg 3660 aaatagcatt tggacaaaag gttattaaag aaaaatcaga tatgaataat cataattcag 3720 agtctaaaat gaagatcatt catggagatg taaacatagg ggttcacgga aaagatttca 3780 aggctatatt ctctaaacaa gagggaggaa tcgtatcctt gagatataat aataaggagt 3840 ttataacgag aacgccaaaa actttctatt ggagagcaac aacagataat gatagaggaa 3900 atagacatga atttagatgc agtcaatggc tggctgctac tatggggcag aagtatgtgg 3960 atttttcagt tgaggaattt gatgagaaga ttacattata ttatacttat caattgccaa 4020 cagtgccatc tactaatgtt aagataactt atgaagtatc tggagaagga ataattaaag 4080 taaatgttaa gtataaagga gttagcggat tacctgaatt gcctgtacta ggaatggatt 4140 ttaaattatt agccgaattt aattcattta gctggtatgg aatggggcca gaagaaaact 4200 atatagacag atgtgaaggt gcaaaacttg gaatatatga gagtacacaa tagaaaatct 4260 atcaaggtat ttagtaccac aagaatgtgg taacaggata ggaactagat gggtagtagt 4320 taaaaatcat aagaatgaag gtcttaaatt tacttatgtt aaagttccat ttgaatttag 4380 tgttttacca tacagcagca tggaattaga aaattcactt catatagaag aattaccatc 4440 tgttaatttt acacattgtg aatataatag gtaaacaaat gggtgttggc ggagatgcaa 4500 tgctggggag caccatgata cctaaattct gtatagattc aagtaaggat ttagaatata 4560 gttttataat ttctaaaatt atactacgca catatgggaa ctatagatat ccaaaacaaa 4620 acttagactt atgcaataat ttacgaaagg acaggtactc tgttgtttcg gttactaaga 4680 ataagttgag gctttctaac atcataagtt gcaccatttc agcatgctcc cgagacaagc 4740 tcgtgacaag caaaaatgga acaacttatg atgaagaaat gcctgcaaca tattctttaa 4800 tgtaacactg cacaaaagag tacctgtcct ttctgatata gcagattttt caagctataa 4860 gtatatctca cgaaatcata aatattttga ttccgaaaag ctatgaaaat atcgctgaag 4920 gttctaagca gctggttgtg tgcaccttag catgctccaa ctttcagttt gacaagctaa 4980 aatggaacaa tctacagctc aagaaacttt aacagctcat tttcaaatgt tttctacaca 5040 aatatattta tatttctagt gaagatatga aattaaattt ttagcgactt tgtaaatatg 5100 ttaatctaat atacgaattc 5120 // |
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