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EBI DbfetchID M35097; SV 1; linear; genomic DNA; STD; PRO; 7956 BP. XX AC M35097; X07754; XX DT 31-JUL-1991 (Rel. 28, Created) DT 17-APR-2005 (Rel. 83, Last updated, Version 5) XX DE Ralstonia eutropha chlorocatechol degradative operon, complete sequence. XX KW . XX OS Cupriavidus necator OC Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales; OC Burkholderiaceae; Cupriavidus. OG Plasmid pJP4 XX RN [1] RP 1-1598 RX DOI; 10.1093/nar/16.14.7200 RX PUBMED; 3405772. RA Perkins E.J., Bolton G.W., Gordon M.P., Lurquin P.F.; RT "Partial nucleotide sequence of the chlorocatechol degradative operon RT tfdCDEF of pJP4 and similarity to promoters of the chlorinated aromatic RT degradative operons tfdA and clcABD"; RL Nucleic Acids Res. 16(14B):7200-7200(1988). XX RN [2] RP 1-7956 RX PUBMED; 2185214. RA Perkins E.J., Gordon M.P., Caceres O., Lurquin P.F.; RT "Organization and sequence analysis of the 2,4-dichlorophenol hydroxylase RT and dichlorocatechol oxidative operons of plasmid pJP4"; RL J. Bacteriol. 172(5):2351-2359(1990). XX DR GOA; P42427. DR HSSP; 1IZ1; Q9WXC7. DR InterPro; IPR011991; Wing_hlx_DNA_bd. DR UniProtKB/Swiss-Prot; P42427; TFDT_RALEJ. XX CC [Unpublished (1988) Dept of Biochem,Univ of Wash.,Seattle,WA 98195] CC enum. 1 to 1598. XX FH Key Location/Qualifiers FH FT source 1..7956 FT /organism="Cupriavidus necator" FT /plasmid="pJP4" FT /mol_type="genomic DNA" FT /db_xref="taxon:106590" FT CDS 337..1104 FT /codon_start=1 FT /transl_table=11 FT /gene="tfdC" FT /product="chlorocatechol 1,2-dioxygenase" FT /db_xref="GOA:P0A396" FT /db_xref="HSSP:1DMH" FT /db_xref="InterPro:IPR015889" FT /db_xref="UniProtKB/Swiss-Prot:P0A396" FT /protein_id="AAA98262.1" FT /translation="MNKRVKDVVDAIVAAVQRVLDQKEVTEAEYRTAVHYLMQVAEQRE FT TALLCDVFFNSTVAATKARISEGSTPAIEGPYYRDDAPLVDDRLKTYDTDDHKPLLIQG FT TVKAVDGSVVEDVTIDVWHSTPDGKYSGFHDDIPTDFYRGKLRVGTDGSFRVRTTMPVP FT YQIPDQGPTGALLETMGGHSWRPAHVHFKVKAPGYETLTTQYYFEGGDWITDDCCNGVQ FT SSLITPDIVEEGVRLMNINFVIEPARAQAGANP" FT CDS 1101..2213 FT /codon_start=1 FT /transl_table=11 FT /gene="tfdD" FT /product="chloromuconate cycloisomerase" FT /db_xref="GOA:P05404" FT /db_xref="InterPro:IPR013370" FT /db_xref="PDB:2CHR" FT /db_xref="UniProtKB/Swiss-Prot:P05404" FT /protein_id="AAA98263.1" FT /translation="MKIDAIEAVIVDVPTKRPIQMSITTVHQQSYVIVRVYSEGLVGVG FT EGGSVGGPVWSAECAETIKIIVERYLAPHLLGTDAFNVSGALQTMARAVTGNASAKAAV FT EMALLDLKARALGVSIAELLGGPLRSAIPIAWTLASGDTKRDLDSAVEMIERRRHNRFK FT VKLGFRSPQDDLIHMEALSNSLGSKAYLRVDVNQAWDEQVASVYIPELEALGVELIEQP FT VGRENTQALRRLSDNNRVAIMADESLSTLASAFDLARDRSVDVFSLKLCNMGGVSATQK FT IAAVAEASGIASYGGTMLDSTIGTSVALQLYSTVPSLPFGCELIGPFVLADTLSHEPLE FT IRDYELQVPTGVGHGMTLDEDKVRQYARVS" FT CDS 2288..2992 FT /codon_start=1 FT /transl_table=11 FT /gene="tfdE" FT /db_xref="GOA:P27136" FT /db_xref="HSSP:1DIN" FT /db_xref="InterPro:IPR002925" FT /db_xref="UniProtKB/Swiss-Prot:P27136" FT /protein_id="AAA98264.1" FT /translation="MLSDGVEITSRSGGRFGAYLGKPTTDSAPIVVIAQEIFGITPFIR FT ETVEWLVGAGFGCVCPDLYWRQAPNIELDANVPSEREQALALFRDFDMEAGVNDLSCAI FT EYARALPFSNGRVAVVGYCLGGALAFDVAARSLADCSIGYYGVGLEKKVSLVPAITRPA FT MFHMGTKDHYVTEEARSILEEHFGRNKNLSLHWYPVGHSFARSSSPNFDQAATTVANAR FT TLELLAMLKDPS" FT CDS 2989..4053 FT /codon_start=1 FT /transl_table=11 FT /gene="tfdF" FT /db_xref="GOA:P27137" FT /db_xref="InterPro:IPR018211" FT /db_xref="UniProtKB/Swiss-Prot:P27137" FT /protein_id="AAA98265.1" FT /translation="MKKFTLDYLSPRVVFGAGTASALPDEIGRLGARRPLVLSSPEQRE FT LAKDIVRPIGDRVAGYFDGATMHVPVDVIQKAERAFNDTDADSIIAIGGGSTTGLAKIL FT SMNLDVPSLVIPTTYAGSEMTTIWGVTEGGMKRTGRDPKVLPKTVIYDPLLTVDLPLAI FT SVTSALNAIAHAAEGLYSADLNPVLETMCKQGICALFDAIPRLVAKPTDAEARTDALFG FT AWMCGTALCHLGMGLHHKLCHTLGGTLNLPHAETHAIVLPHALAYNLPYAAPAERLLQE FT VAGSSDVPSALYDLARNAGAPLSLAEIGMRPEDIPRVRDLALRDQYPNPRPLESDALET FT LLVNAFRGRRPDFK" FT CDS 4398..6194 FT /codon_start=1 FT /transl_table=11 FT /gene="tfdB" FT /product="2,4-dichlorophenol hydroxylase" FT /db_xref="GOA:P27138" FT /db_xref="InterPro:IPR003042" FT /db_xref="UniProtKB/Swiss-Prot:P27138" FT /protein_id="AAA98266.1" FT /translation="MALTIETDVLVVGTGPAGASAGALLARYGVRTMLINKYNWTAPTP FT RAHITNQRTMEILRDLGLEAEARLYAAPNDLMGENTICASLAGEEFGRIRTWGTDVRRR FT ADYDECSPTSMCDLPQNYLEPILVKSAALDGCKVRFDTEYLGHEQDADGVSSRLRDRLN FT GEEFTVRSKYLIGADGANSRVVSDLDLPLEGTMGKSGSINLLFEADLDRYVAHRPSVLY FT WVIQPGSDIGGLGIGVVRMVRPWNKWLAIWGYDVEQGPPEISESFARRIVHNLIGDDSV FT PLKIEGISTWTVNDMYATRLQQGRVFCAGDAVHRHPPTNGLGSNTSIQDSFNLAWKIAM FT VLNGTADESLLDTYTIERAPIAKQVVCRANKSLEDFPPIAMALGLPQAKSADEMKSNMA FT RRKEPGPEAQAQRTRLREAIAGTNYVYNAHGVEMNQRYDSPAIVADNSPDEVFRDVELY FT HQASTRPGAPMPHVWVYASGDGHRISTKDLCGKGNFTLFTGIGGAAWQDAAAAVSRQLG FT VAVTVRIIGPGQAYEDHYGDFARISEIIDTGAILVRPDFHVAYRATSLPADAAGDLVSA FT MRRILGRQSERSSALRVTSRAI" XX SQ Sequence 7956 BP; 1716 A; 2209 C; 2403 G; 1628 T; 0 other; aagctttgcc ccaatatcgc gttccaaggc ctggatctga cgagtaagcg gtggctgcga 60 tatgtgcatc ctctgtgccg ctgttccgaa acctcccgcc tccgcgaccg cgacgaagta 120 tttcaactgt cttatttcca tgttccgtcc cgttgcgctg cctgttacaa ggcgattgtg 180 agcaagcacc tgcggtctcg caactaggga aagacatacc gaaaccgtat ggtctaggcg 240 gagaaaaggt attggacggt atgggattgc cgtctctatt atgactgtgc ggggcaattg 300 tcgcccacgt gttcatgttt tcatgacgga ggcaaagtga acaaaagagt caaggatgtt 360 gtcgatgcga tcgtcgcggc ggtacagcgg gtgctcgacc agaaggaggt tactgaggcg 420 gaataccgaa ctgcggttca ttacctcatg caagtagccg aacagcgtga aaccgctctt 480 ctttgcgatg tttttttcaa tagcaccgtg gctgcgacga aggctcgtat tagcgaaggt 540 tcaacgcctg ctatcgaggg accctactat cgcgacgacg cccctctggt cgatgaccgg 600 ctcaagactt acgacacgga cgaccacaag cctttgctta tccagggaac ggtcaaagcg 660 gtcgacggga gcgttgtcga ggacgtgacg attgatgtct ggcattcgac gcccgatggg 720 aagtacagcg gtttccatga cgacatcccg actgattttt atcgagggaa gctcagggtg 780 ggcaccgatg gcagcttccg cgtgcgcaca acgatgccgg tgccgtatca gatcccggat 840 cagggtccca cgggcgcatt gctcgaaacc atgggtggtc actcgtggcg tcccgctcat 900 gtacatttca aggtgaaggc gccgggatat gaaacgttga ccacgcagta ctacttcgaa 960 ggtggagatt ggatcacgga cgactgttgc aacggcgtcc agtctagtct gattactccc 1020 gatattgtgg aagagggtgt ccgcctgatg aacatcaact tcgttatcga gcccgcgcgt 1080 gcgcaggcgg gggcaaaccc gtgaagattg acgcgattga agcagtgatc gtggatgtgc 1140 cgaccaagcg gccgatccag atgtcgatca ctaccgtgca ccagcagagc tacgttatcg 1200 tccgggtgta ttcggagggg ctcgttggtg tcggcgaggg tggaagcgtt ggtggtcccg 1260 tctggagcgc agagtgtgcg gagacgatca agatcatcgt ggaacggtat ctcgcgcccc 1320 acctcctcgg aactgatgcg ttcaacgttt caggtgcact gcaaaccatg gcgcgtgccg 1380 tcaccggaaa cgcctctgca aaggctgcgg tcgagatggc gttactggat ctcaaagctc 1440 gagcgttagg cgtatcgatc gccgagttac ttggcgggcc gttgcgcagt gcgattccga 1500 ttgcctggac attggcgagc ggagatacga aacgcgatct cgattctgcc gtcgagatga 1560 ttgaaagacg acgacacaat cgcttcaaag tcaagcttgg cttccggtcg ccccaagacg 1620 atctcatcca tatggaggct ttgtcaaata gtctcggatc gaaggcctac cttcgcgttg 1680 acgtgaatca ggcttgggac gagcaagtgg cgtccgtcta cattcctgaa ctggaggcgc 1740 ttggcgtgga actcatcgaa cagccggtcg gccgcgaaaa tacgcaagcg ttgaggcggc 1800 tctccgacaa caaccgcgtg gccatcatgg ccgatgagag cctgagtacg ttggcctcgg 1860 cattcgatct cgcgcgcgac cgaagtgtgg atgtcttttc gctgaagctt tgcaacatgg 1920 gaggggtctc ggcgacgcaa aagatagcag cggtcgcgga agcaagcggg attgcatcgt 1980 atggcggaac aatgcttgac tcgacgatcg gcacatcggt tgcacttcag ctctattcta 2040 cggttccatc gcttccgttc ggttgcgaac tgatcggtcc cttcgtgttg gcagacacgc 2100 tgagccacga gccactcgag atccgggatt acgaactgca ggttcccact ggcgtaggtc 2160 acggcatgac gcttgacgag gacaaggtgc gccaatacgc acgcgtcagc taggcaaaac 2220 gcgcagcgca ggccgcggct gtggatcgcc tcgaatgcaa ctgcgcatgt gtaactggag 2280 actaaatatg ttatcagacg gcgttgagat cacgtcgcgc tcgggtggtc gctttggtgc 2340 ctacctcgga aagccgacga cggactccgc acccatcgtc gtgatcgcgc aggagatttt 2400 cgggatcacg cctttcataa gagaaaccgt ggaatggctc gttggtgcgg gctttggttg 2460 cgtgtgtccg gatctgtact ggagacaggc gccgaatatc gagcttgatg caaacgtacc 2520 atcggaacgg gaacaggcgc ttgcgttgtt tcgcgacttt gacatggagg caggagtcaa 2580 tgacctttca tgcgccattg aatacgctcg tgcgctccct ttctcgaacg gtcgtgtcgc 2640 tgtagtcggg tactgcctgg gaggtgcgct ggcgtttgat gtggcggcgc gatcattggc 2700 cgattgctca atcggctatt acggcgtggg acttgaaaaa aaggtctcac tggtgccagc 2760 tattacacga ccggcgatgt tccacatggg taccaaagat cactatgtca cagaagaggc 2820 ccgtagcatt ctcgaagagc atttcggtcg aaacaaaaat ctgagtctgc actggtatcc 2880 agtggggcat tcatttgcac ggtcatccag cccaaatttc gatcaggcgg cgacaactgt 2940 ggccaatgct cggacgctcg aactgcttgc aatgttgaag gacccttcat gaagaagttc 3000 acgcttgact acctgagccc gagggtcgtc ttcggggcgg gcactgcttc tgcattgcca 3060 gatgaaatag gacgccttgg cgcacgccgg cccttggtat taagcagccc ggaacaacgc 3120 gagttagcga aggatatcgt ccgtccgata ggtgacaggg tagctggata tttcgatggc 3180 gcgacgatgc atgttcccgt cgacgtcatc cagaaagccg agcgggcttt taacgatact 3240 gacgccgact caatcatcgc gatcggggga ggatcgacca ccggactcgc aaaaatcctt 3300 tcgatgaacc ttgacgtccc aagtctggtt ataccaacga cctatgccgg tagtgaaatg 3360 actaccattt ggggtgtcac ggaaggcgga atgaagagga ccggccgcga ccccaaggtg 3420 ctaccgaaga ccgtgattta tgatccattg ctcacggtcg atttgccgct tgctatctcg 3480 gtgacgagcg ccttgaatgc gatcgctcac gccgcagaag gtctgtactc ggccgacctc 3540 aatcccgttc tcgagaccat gtgtaagcag ggcatatgcg ccttgttcga tgcaatcccg 3600 cgcctggtgg caaagccgac tgacgccgaa gcgcgtacgg atgccctttt tggggcatgg 3660 atgtgtggca ctgcactgtg ccacttgggc atggggctac atcacaaact ctgccacacg 3720 cttgggggaa cccttaatct tccccacgcg gagacacatg caatcgtact accacacgca 3780 ctggcataca atctgccgta cgccgcgcca gctgagcgac tgcttcagga agtcgccggc 3840 agtagtgacg tcccgagcgc gctatatgat ctcgccagaa atgctggagc accactcagt 3900 ctcgccgaaa tcggtatgcg gcctgaagat attccgaggg tacgcgacct cgcgctaagg 3960 gaccaatatc cgaatccgcg tccgctggaa tcggacgcat tggaaacatt gttagtcaat 4020 gcgtttcgtg ggcgaagacc ggatttcaaa taatgtgacc tgcactccgc gtttagtacg 4080 gtagcggtga agagcccgtt ccaaagtcga catcgggtct tcaagctaaa gcgaagcaca 4140 tgaatttgca tccactgttc cttgtggagc atcgccagcc ttcgcgcgaa aaaggccgac 4200 tgtactggag gtggctcgga ttttctttgc gcgaagtggc tcatttttac tttgcgcgca 4260 acaaaggctc ttcagcctta ctattcatcg cgcgttaaag acggtgaagt ttaaaaaatc 4320 gacttggccg aacgcgagat gaacaatccc tgtttcttct cacaaagaga gagacataac 4380 aattgacgga ggaagacatg gcattgacga tcgaaaccga cgtgctggtc gttggcaccg 4440 ggccggcggg cgcttcggcg ggggcactgc ttgcgcgata cggtgtcagg acgatgctca 4500 ttaacaagta caactggacg gctccgacgc cgcgtgccca catcaccaac caacgcacaa 4560 tggagatcct gcgtgatctc ggtctcgaag ctgaagcccg cctctacgcc gcgccgaatg 4620 acctaatggg cgagaacacc atctgcgcct cgttggccgg cgaggagttc ggtcggattc 4680 gcacctgggg gacggatgtt cgccggcgcg ccgattacga cgaatgttcg ccgacctcca 4740 tgtgcgacct gccacagaac tatctcgagc cgattctcgt caagtcggcc gcactcgacg 4800 gctgcaaggt gcgcttcgac acagagtatc ttggacacga acaggatgct gacggcgtgt 4860 cgtcgcgctt gcgcgatcgt ctgaatggcg aagagtttac cgtccggtcg aaatatctga 4920 tcggcgccga cggcgccaac agccgcgtcg tgtcagacct ggacctgccg ctcgaaggta 4980 ccatgggcaa gtccggttcc atcaacttgc tgttcgaggc tgatctcgac cgctacgtcg 5040 cgcatcgtcc atcggtcctt tactgggtca tccagcccgg ctccgatatc ggcggtctcg 5100 gcatcggcgt ggtgcgcatg gtccggccgt ggaacaaatg gcttgcgatc tgggggtacg 5160 acgtcgagca gggcccgccg gagatctcgg aatccttcgc ccgccgcatc gtccacaatc 5220 tgatcggcga tgacagcgtg ccgctgaaaa ttgagggcat ctcgacctgg acggtcaacg 5280 acatgtacgc gacccggttg cagcaaggac gggtcttctg cgccggcgac gcggtccacc 5340 ggcatccgcc gaccaacgga ctcggctcaa acacctccat ccaggacagc ttcaatctcg 5400 cctggaagat cgccatggtg ctgaacggca cggccgacga gagcctgctg gacacctaca 5460 cgatcgagcg ggcgccgatc gcgaagcagg tggtctgcag ggcgaacaag tcgcttgagg 5520 atttcccgcc gatcgcgatg gctctggggt tgccgcaggc caagagcgcc gacgagatga 5580 agagcaacat ggctcgccgc aaggaacctg ggcccgaggc gcaagcgcag cgcacgcggt 5640 tacgcgaggc catcgccggg accaattacg tttacaacgc ccacggcgtc gaaatgaatc 5700 agcgttacga cagtcccgcg atcgtggcag acaattcgcc ggacgaagtc ttccgcgacg 5760 tcgagctcta tcaccaggcg tcgacgcggc ctggagcgcc catgccgcat gtctgggtct 5820 atgcgagtgg tgatggacac cgtatctcaa caaaggatct ctgcgggaag ggcaacttca 5880 ctctctttac cgggattggc ggcgcagcct ggcaggatgc cgcggcggcc gtgagccggc 5940 aactcggggt ggcagtgacg gtgcgcatca ttgggcctgg ccaggcctac gaggatcatt 6000 acggcgactt cgcgagaatt tccgagatca tcgacaccgg cgccatcctg gtgcggccgg 6060 atttccacgt cgcctatcgg gcgacgtcct tgccggccga tgcggcaggc gacctcgtgt 6120 ccgccatgag gcggatcctc ggacgccagt cagagaggtc gagcgcgctg cgcgttacgt 6180 ctcgagctat ataaggagtg gcgtcgcgat ctattcgagg tgagacggaa gcgctcacct 6240 cgggttgcgg gaggggaagc gtgatcttgc gcagcgtgag atggcccgtg gcgacgcggg 6300 aaggttgtcg cgcgattaag gcaaggcggt gcgcaacgac gtgacgtgtt ttctggaccg 6360 gacgtaactg cagatcatct aatttgggaa gttcgtaccg gtgtcgaccg ccttgctgat 6420 gatgctatta agcacgtgag cagacgacgg gccggtgcag ccaacgttga aaaaagagcg 6480 cttgattgca atgtcgacga cacgatctac attactgtca tttgaacgac cgcttgctcg 6540 tgcggattca accggtggat gcaacagttt gctgaaatcg ttcggcgggc gttccgtggt 6600 tcagggtttt cctcggtcgc ccatccagcc gcctcgccac ggtattgagt agccaattgg 6660 tacaccgaaa tgtaggtgcc gttggggatg tctcgatagc ccgttcgcat tttcattcga 6720 cccgcgctgc caaggatgct gggggttcag taactgcagg ctgaactgcg ccaggtgaac 6780 caaacgctga tcgtcaagca aaatgaaata acccagttga acagagaaag cgcgcggctc 6840 gtcgccgaag ccggcgtcgc gacaaggcat ctacgcgagc tgcaagcgca cagcaagtag 6900 ttacagaata cgttgaacca gtcgctcgcc gaccacgcac gcaccaaggc cgagcacgat 6960 gcgctgctaa caccgcgagg actgaggcga ctgaactgac gcggctgcgc gaagcacgtg 7020 aatcgctgac cgccgatctg acgaagctca cagcgcagtt cgacgagcag cagttgctcg 7080 gggactatcg gatgcgtctg ggcctcacgg acgcggcggg atgaaagcgc cagattccgc 7140 atcaaaagga tggggatcta cgccggaacc ccagaaaatt ccgtcgttcc acattgtgaa 7200 cattaatcgg cttattccag ccgaagaatg ttggagcgac gtccggtgag tgacatgacg 7260 gtctcggtca cacggaccat caggaccgtt acgcggggcc cagttcgatt acattgtggg 7320 acaggtcgag acaatcttcg cagtcccggc aaacccagtg cagcatcggc ccgaccggat 7380 ctggatggtc gctgaacgtt gtcagcgccg ggtcgtacta gcatcggacg tgctcgagcg 7440 cagtatccgc tcgagattag actcgatgcg gtccaggctg cattcaccga tgccggactt 7500 gaacaccgtc acaccgcctg acgggttgac cgcgaagaag aaaaacgcct tcgtgtcgac 7560 catgtgcgac aggacttgtc ctggcgatga agttgctcgg attgatccag cgcattgaag 7620 atgcggtagc cgtaaaactc taagccgaag tgtacgacct cgaagtcgtc ggtgccgcgg 7680 atcgagtggc caaagttgaa accgctgcgt gttacgtcgt tcggaatgtc atccaccggc 7740 cgcagtgcca ggcaagcata gtgcgtctgg ccctcggcca cgagtcccgt cgcaaagcag 7800 ggcgcgcgct cggccagcaa ggcctcgaca aaattccggt tcatgcggag cgttggcagc 7860 atcgggcatc cttcatcgtg aatcaaatca acatttgccg acctggctgt gcggtgcgtc 7920 gacgtcgaac atcaaaggct atacatcgtc gagctc 7956 // |
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