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EBI DbfetchID M35089; SV 1; linear; genomic DNA; STD; PRO; 2735 BP. XX AC M35089; X58897; XX DT 25-JUL-1990 (Rel. 24, Created) DT 17-APR-2005 (Rel. 83, Last updated, Version 5) XX DE B.stearothermophilus branching enzyme (glgB) gene, complete cds. XX KW branching enzyme. XX OS Geobacillus stearothermophilus OC Bacteria; Firmicutes; Bacillales; Bacillaceae; Geobacillus. XX RN [1] RP 1-2735 RX DOI; 10.1007/BF00290661 RX PUBMED; 1745226. RA Kiel J.A.K.W., Boels J.M., Beldman G., Venema G.; RT "Molecular cloning and nucleotide sequence of the glycogen branching enzyme RT gene (glgB) from Bacillus stearothermophilus and expression in Escherichia RT coli and Bacillus subtilis"; RL Mol. Gen. Genet. 230(1-2):136-144(1991). XX CC Draft entry and computer-readable sequence for [Unpublished (1990)] CC kindly submitted CC by J.A.K.W.Kiel, 12-JUN-1990. CC Dept of Genetics CC Center of Biological Sciences CC Kerklaan 30, NL 9751 NN CC Haren, THE NETHERLANDS. XX FH Key Location/Qualifiers FH FT source 1..2735 FT /organism="Geobacillus stearothermophilus" FT /strain="1503-4R variant 4" FT /mol_type="genomic DNA" FT /clone="pKVS1" FT /db_xref="taxon:1422" FT CDS complement(<1..325) FT /codon_start=1 FT /transl_table=11 FT /note="unidentified ORF2" FT /db_xref="GOA:P30540" FT /db_xref="InterPro:IPR002524" FT /db_xref="UniProtKB/Swiss-Prot:P30540" FT /protein_id="AAA22481.2" FT /translation="MSPIQYDYHHLPHVKTQNQSKKTLWITLVLTLFFTIVEIVGGLLS FT NSLALLSDSAHMASDVLALGLSMIALYLAMRPPNHRFTFGYLRFEIITSFLNGLTLAII FT SIGI" FT RBS complement(330..337) FT /note="ORF2 ribosomal binding site (put.); putative" FT -10_signal complement(365..370) FT /note="ORF2 -10 region (put.); putative" FT -35_signal complement(389..394) FT /note="ORF2 -35 region (put.); putative" FT -35_signal 446..458 FT /note="glgB -35 region (put.); putative" FT -10_signal 469..479 FT /note="glgB -10 region (put.); putative" FT RBS 504..516 FT /note="glgB ribosomal binding site (put.); putative" FT CDS 522..2441 FT /codon_start=1 FT /transl_table=11 FT /note="branching enzyme (glgB) (EC 2.4.1.18)" FT /db_xref="GOA:P30538" FT /db_xref="InterPro:IPR004193" FT /db_xref="InterPro:IPR006047" FT /db_xref="InterPro:IPR006048" FT /db_xref="InterPro:IPR006407" FT /db_xref="InterPro:IPR013780" FT /db_xref="InterPro:IPR013781" FT /db_xref="InterPro:IPR013783" FT /db_xref="InterPro:IPR017853" FT /db_xref="UniProtKB/Swiss-Prot:P30538" FT /protein_id="AAA22482.1" FT /translation="MIAVGPTDLEIYLFHEGSLYKSYELFGAHVIKKNGMVGTRFCVWA FT PHAREVRLVGSFNEWNGTNFNLMKVSNQGVWMIFIPENLEGHLYKYEITTNDGNVLLKS FT DPYAFYSELRPHTASIVYNIKGYQWNDQTWRRKKQRKRIYDQPLFIYELHFGSWKKKED FT GSFYTYQEMAEELIPYVLEHGFTHIELLPLVEHPFDRSWGYQGIGYYSATSRYGTPHDL FT MYFIDRCHQAGIGVILDWVPGHFCKDSHGLYMFDGAPAYEYANMQDRENYVWGTANFDL FT GKPEVRSFLISNALFWMEYFHVDGFRVDAVANMLYWPNSDVLYKNTYAVEFLQKLNETV FT FAYDPNILMIAEDSTDWPRVTAPTYDGGLGFNYKWNMGWMNDILTYMETPPEHRKYVHN FT KVTFSLLYAYSENFILPFSHDEVVHGKKSLLSKMPGTYEEKFAQLRLLYGYLLTHPGKK FT LLFMGGEFGQFDEWKDLEQLDWMLFDFDMHRNMNMYVKELLKCYKRYKPLYELDHSPDG FT FEWIDVHNAEQSIFSFIRRGKKEDDLLIVVCNFTNKVYHGYKVGVPLFTRYREVINSDA FT IQFGGFGNINPKPIAAMEGPFHGKPYHIQMTIPPFGISILRPVKKGSVKSFMKTPHPPS FT HGAS" XX SQ Sequence 2735 BP; 835 A; 492 C; 634 G; 774 T; 0 other; gaattccaat ggaaataatg gctaacgtaa ggccgtttaa aaaggacgta ataatttcaa 60 agcgcaaata accgaatgta aatcgatgat ttggcggacg catggcaaga taaagagcga 120 tcatgctaag cccaagcgcc aatacgtcag atgccatatg ggcagagtcg gaaagcaaag 180 ctaaggaatt ggataatagc cccccaacaa tttccacaat cgtaaaaaac aatgttaaaa 240 cgagagtgat ccaaagcgtt tttttcgatt gattttgcgt ttttacatga ggaagatggt 300 gataatcgta ttgaattggt gacatgacac acctcttatt tagaattatt tttaatttat 360 atacattata atatagtttt ttataattgt gcaaaaaaat tttttgttta tttatcgaaa 420 aatgtaaaaa aaatacaatt tttttatcaa ggaatttatg gaatcgctgt ggaatataag 480 taacaacggt aagaaacttt aaggaaagga tgcgatacag attgatcgcc gtcggtccca 540 ctgatttaga aatctattta tttcatgaag gcagcttata taaaagttat gaattgtttg 600 gtgcacatgt gataaagaaa aatggcatgg tcggaacccg gttttgtgta tgggcacccc 660 atgcgcggga agtgcgatta gtcggcagtt ttaatgaatg gaacggaact aattttaacc 720 ttatgaaagt aagtaatcaa ggcgtatgga tgatttttat tcctgaaaac ttagaagggc 780 atttatataa atacgaaatt acgacgaacg atgggaatgt tctgttaaaa tcggatccat 840 acgcgtttta ctccgagttg cgtccccata ctgcttccat tgtctacaac ataaaaggat 900 atcaatggaa tgaccagaca tggcgacgga agaaacagcg aaagcgaatt tatgaccagc 960 ctttgttcat ttatgaactt cactttggtt cgtggaaaaa gaaagaggac ggcagttttt 1020 atacatatca agagatggca gaggagctaa tcccttatgt tctcgaacat gggtttactc 1080 atattgagct gctcccactc gtcgagcatc cgttcgatcg ttcttgggga tatcagggaa 1140 taggttatta ttcagcaaca agccgctacg gaacaccgca tgatttgatg tattttattg 1200 accgctgtca ccaagctgga ataggcgtca ttctcgattg ggttcctggc cacttttgta 1260 aagattccca tgggttatat atgtttgatg gcgcaccggc atatgaatat gccaacatgc 1320 aagaccggga aaattacgta tggggaacgg caaactttga ccttggcaag ccggaagtcc 1380 gcagcttttt gatttccaat gcgttatttt ggatggaata tttccatgtg gacgggtttc 1440 gtgtagatgc tgttgccaat atgttatatt ggccaaacag cgacgtacta tacaaaaata 1500 cgtatgccgt ggagttcttg caaaaattaa atgaaacggt attcgcctat gatccgaaca 1560 tattaatgat tgccgaagat tcgacagact ggccgcgcgt cactgctcca acatacgacg 1620 gaggattagg atttaactat aaatggaaca tgggatggat gaacgatatt ttaacttata 1680 tggaaacgcc gcctgaacat cgaaaatacg tgcacaataa agtaacattt tccctcttgt 1740 atgcgtattc ggaaaatttc attttacctt tttcccatga cgaggtcgta catggaaaaa 1800 aatcgctgtt aagtaaaatg ccggggacat atgaggaaaa gtttgcgcaa ttaaggttgc 1860 tgtatggata tttgttgacg catcctggta agaaattatt gtttatgggc ggcgaatttg 1920 gccagtttga tgaatggaaa gatttagagc agctggattg gatgcttttt gattttgata 1980 tgcatcggaa tatgaatatg tatgtgaaag aattgttgaa atgttataag cgctataaac 2040 cgctttatga gttagaccac tctccagatg gattcgagtg gattgatgtt cataacgccg 2100 aacaaagtat tttctcattc attcgcagag gaaaaaaaga ggatgatttg cttattgttg 2160 tgtgtaattt cacaaataaa gtataccacg gttataaagt tggtgttccg ttatttacaa 2220 gatatcggga agtaatcaat agcgatgcaa tccaattcgg cggctttggg aatatcaatc 2280 caaaaccgat tgcggcgatg gaagggccgt ttcacggaaa gccatatcat attcagatga 2340 cgatcccgcc gtttggcatt tctattttaa gaccagtaaa aaaaggtagc gtcaaaagtt 2400 ttatgaaaac tccacatccg ccatcccatg gagcatcgta aggcatcctt ggagccggat 2460 tcgcccttga ccaacacccg ccaaaggtgt gaaagggacg tcaagggcga cggggacaaa 2520 aaagagggca taggaaagcc gcccttgccc ttaccgaatt ttacctttga cgaggttcgg 2580 ttggtcaagg gttcgcttcg ccgaatccgg ctgttcttct gatccatggg ctccggcgga 2640 caaaaaagtt aggctgcctc ttgttggagg aaatcttgag ccatggcgat cagcttcgtc 2700 caccgggccg gcatatgggg cagatcggcg agctc 2735 // |
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