 |
|
EBI DbfetchID M34459; SV 1; linear; genomic DNA; STD; PRO; 6067 BP. XX AC M34459; XX DT 10-JUL-1990 (Rel. 24, Created) DT 17-APR-2005 (Rel. 83, Last updated, Version 5) XX DE C.saccharolyticum xylanase A (XynA), beta-xylosidase (XynB) and acetyl DE esterase (XynC) genes, complete cds. XX KW acetyl esterase; beta-xylosidase; xylanase. XX OS Caldicellulosiruptor saccharolyticus OC Bacteria; Firmicutes; Clostridia; Thermoanaerobacterales; OC Thermoanaerobacterales Family III. Incertae Sedis; Caldicellulosiruptor. XX RN [1] RP 1-6067 RX PUBMED; 2111111. RA Luethi E., Love D.R., McAnulty J., Wallace C., Caughey P.A., Saul D., RA Bergquist P.L.; RT "Cloning, sequence analysis, and expression of genes encoding RT xylan-degrading enzymes from the thermophile 'Caldocellum saccharolyticum"; RL Appl. Environ. Microbiol. 56(4):1017-1024(1990). XX FH Key Location/Qualifiers FH FT source 1..6067 FT /organism="Caldicellulosiruptor saccharolyticus" FT /mol_type="genomic DNA" FT /db_xref="taxon:44001" FT sig_peptide 195..293 FT /note="xylanase/beta-xylosidase signal peptide" FT CDS 195..1223 FT /codon_start=1 FT /transl_table=11 FT /note="xylanase/beta-xylosidase (XynA) precursor" FT /db_xref="GOA:P23556" FT /db_xref="HSSP:1HIZ" FT /db_xref="InterPro:IPR013781" FT /db_xref="UniProtKB/Swiss-Prot:P23556" FT /protein_id="AAA23059.1" FT /translation="MRCLIVCENLEMLNLSLAKTYKDYFKIGAAVTAKDLEGVHRDILL FT KHFNSLTPENAMKFENIHPEEQRYNFEEVARIKEFAIKNDMKLRGHTFVWHNQTPGWVF FT LDKNGEEASKELVIERLREHIKTLCERYKDVVYAWDVVNEAVEDKTEKLLRESNWRKII FT GDDYIKIAFEIAREYAGDAKLFYNDYNNEMPYKLEKTYKVLKELLERGTPIDGIGIQAH FT WNIWDKNLVSNLKKAIEVYASLGLEIHITELDISVFEFEDKRTDLFEPTPEMLELQAKV FT YEDVFAVFREYKDVITSVTLWGISDRHTWKDNFPVKGRKDWPLLFDVNGKPKEALYRIL FT RF" FT mat_peptide 294..1220 FT /note="xylanase/beta-xylosidae" FT CDS 1257..2057 FT /codon_start=1 FT /transl_table=11 FT /note="acetyl esterase (XynC)" FT /db_xref="GOA:P23553" FT /db_xref="InterPro:IPR000801" FT /db_xref="UniProtKB/Swiss-Prot:P23553" FT /protein_id="AAA23060.1" FT /translation="MAIMQINFYSKMLKKNTTILAILPVDKPDKKFQKDVDSENLKTLY FT LLHGYAGNYMDWLCGARIVELSMRYNVAVFLPSGENSFYLDDEEKEEYFGEFVGNEIIE FT FTRSVFPIPQKREKTFIGGLSMGGYGALRNGLKYNKNFVGIIALSSALIIHKIAGIPKD FT YRNAYASYNYYRRVFGDLNSLIGSDKDINALVTKLKQEKGSIPKIYMACGRDDFLVQEN FT RDLFNFLKNEGIDVVYEEDEGGHDWDFWNKYIANAFEWMSKVSD" FT CDS 2198..2491 FT /codon_start=1 FT /transl_table=11 FT /note="ORF 3" FT /db_xref="UniProtKB/Swiss-Prot:P23554" FT /protein_id="AAA23061.1" FT /translation="MVFFFTSCTIQSAIEQKKTVEEILGKIGESEDKTNSRGQPATMKE FT DEVEDNPLKDVYKDYFLVGAAINGYSVETAAINHPGMAAILKKTLTVQPYLI" FT CDS 2491..3429 FT /codon_start=1 FT /transl_table=11 FT /note="ORF 4" FT /db_xref="GOA:P23557" FT /db_xref="HSSP:1HIZ" FT /db_xref="InterPro:IPR013781" FT /db_xref="UniProtKB/Swiss-Prot:P23557" FT /protein_id="AAA23062.1" FT /translation="MKQQYLLDYEATKASKNGMPVCKFDSCIPALQFCKENGIKMRGHV FT LVWHNQTPEWFFHKDYDVSKPLVDAATMERRLESYIKQVIEFCQKNYPGVVYCWDVVNE FT AILDDGSWREINNNWYTIMKEKYVEKAFYYARKYAKKDVALFYNDYNVFLPAKREAIYN FT LAQKLKEKGLIDGLGLQPTVGLNYPELDSDDIDSFKTTLETFAKLGLQIHITELNFEIK FT GDESNRTPENLKKQADRYYEMMKLLLKEDTDNGGPCNITCVTVFGICDDYPLYKNFKQC FT MYLWDKNCNPKPCFYSFLQAGLDWKASLLSK" FT CDS 3445..4911 FT /codon_start=1 FT /transl_table=11 FT /note="beta-xylosidase (XynB) (3445 could be 3463)" FT /db_xref="GOA:P23552" FT /db_xref="InterPro:IPR013781" FT /db_xref="UniProtKB/Swiss-Prot:P23552" FT /protein_id="AAA23063.1" FT /translation="MERRKIMKITINYGKRLGKINKFWAKCVGSCHATTALREDWRKQL FT KKCRDELGFEYIRFHGWLNDDMSVCFRNDDGLLSFSFFNIDSIIDFLLEIGMKPFIELS FT FMPEALASGTKTVFHYKGNITPPKSYEEWGQLIEELARHLISRYGKNEVREWFFEVWNE FT PNLKDFFWAGTMEEYFKLYKYAAFAIKKVDSELRVGGPATAIDAWIPELKDFCTKNGVP FT IDFISTHQYPTDLAFSTSSNMEEAMAKAKRGELAERVKKALEEAYPLPVYYTEWNNSPS FT PRDPYHDIPYDAAFIVKTIIDIIDLPLGCYSYWTFTDIFEECGQSSLPFHGGFGLLNIH FT GIPKPSYRAFQILDKLNGERIEIEFEDKSPTIDCIAVQNEREIILVISNHNVPLSPIDT FT ENIKVVLKGIENCREVFVERIDEYNANPKRVWLEMGSPAYLNREQIEELIKASELKKEK FT VSWGIVNNNEITFDLSVLPHSVVAVTIKNG" FT CDS 5439..>6067 FT /codon_start=1 FT /transl_table=11 FT /note="ORF 6" FT /db_xref="GOA:P23555" FT /db_xref="InterPro:IPR018392" FT /db_xref="UniProtKB/Swiss-Prot:P23555" FT /protein_id="AAA23064.1" FT /translation="MGLKYIKKAPNYTEIVLKAKIFSTLILMIVILFLINKMPSTYKKA FT YAVVLNNQIVGYVKDKTEAQNLLTQIKKEVEERHNTDSFILQSKLQLKSIEPGQYRETR FT VDELKNTIIEKGKVLVKRYAIFVNSKPYFVFENPQTPNNILNKLKKVYYNDKASQAKFL FT EKVEIKPVYVSPAIKVADEATALTKIMFGKDQVIEYTVKEGDTLWDP" XX SQ Sequence 6067 BP; 2230 A; 787 C; 1243 G; 1807 T; 0 other; ggatccccgc aaagcctaaa ataagtacat ttagaatgat ggcagaaaat ggttatatta 60 cccttgaatt tacgttaagt aaaaatgctg tggtgctttt tgaggtaagc aaggttgtag 120 atgagtcaga tacttatata ggacttgacg atagtaaaat accaggttat tagttgcttt 180 ataaaataaa aggaatgagg tgtttaattg tgtgcgaaaa tttagagatg ctaaacttat 240 cattagcaaa aacatacaaa gattacttta aaataggtgc tgcagtaact gcgaaagatt 300 tagaaggagt tcatagggat attcttttga agcattttaa tagcctcaca ccagaaaatg 360 ccatgaagtt tgaaaatatt catccagaag agcagagata taattttgaa gaggttgcca 420 ggataaaaga gtttgcaatt aaaaatgaca tgaagttaag aggacataca tttgtttggc 480 ataatcaaac tccggggtgg gtgtttttag ataagaatgg ggaagaagcc tcaaaagagt 540 tagttattga aaggttaaga gagcatataa aaactttgtg tgagagatac aaggatgtag 600 tatatgcgtg ggatgtggtg aacgaagcag tagaagataa aacagaaaag cttttgcgag 660 aatcaaactg gagaaaaatt attggagatg attatattaa aattgctttt gagatagcaa 720 gagaatatgc aggagatgca aagttatttt ataacgatta taacaatgaa atgccttata 780 aattagaaaa aacctacaaa gttctaaaag agcttttaga aagaggtact ccaatagatg 840 gaattggtat acaagcacac tggaatatat gggataaaaa tcttgttagt aatttaaaaa 900 aggctataga agtatatgct tccttaggtt tagaaattca tattacagaa cttgacattt 960 cagtatttga gtttgaagat aagaggactg acttgtttga accaaccccg gaaatgcttg 1020 aactacaagc aaaagtatat gaagatgtat ttgcagtttt tcgagaatat aaagatgtaa 1080 taacttctgt tacattatgg ggtattagcg acagacacac atggaaagat aacttccctg 1140 taaagggtcg aaaagattgg cctctcttat tcgacgtaaa tggaaaacca aaagaagcct 1200 tgtacaggat attaagattt taaagatttt ttaacgaaga aaggggttct tttaatatgg 1260 ctatcatgca aatcaacttt tattcaaaga tgttgaaaaa gaacacaaca attttggcca 1320 ttttacccgt agataaacca gataagaaat tccagaaaga tgttgatagt gaaaatttga 1380 aaaccttata tcttttgcat ggttatgctg gtaactacat ggattggttg tgtggagccc 1440 gaattgttga attatcaatg cgatataatg ttgctgtgtt tctgccatca ggtgaaaata 1500 gtttttattt agatgatgaa gaaaaggaag aatattttgg tgaatttgtg ggaaatgaaa 1560 ttatagaatt tacaagaagc gtttttccta ttcctcaaaa aagggaaaaa acttttattg 1620 gcggtttatc aatgggaggt tacggtgctc ttagaaatgg gcttaaatat aacaagaatt 1680 ttgtaggtat aatagcttta tcatcagcac taataattca taagattgca ggtattccta 1740 aggattatag gaatgcttat gcaagttata actattatag acgagtgttt ggagacctaa 1800 actctttaat aggtagcgat aaagacataa atgccttagt tactaagcta aaacaagaaa 1860 aaggtagtat tccaaaaata tacatggcat gcggcagaga tgacttttta gttcaagaaa 1920 acagagattt atttaatttt ttgaaaaatg aaggtataga cgtggtttat gaggaagacg 1980 aaggtggaca tgactgggat ttttggaaca aatatattgc aaatgctttt gagtggatga 2040 gtaaggtttc tgattaagtc ttcacgtacc ctgttttaag ttttacaaat agatttgtgg 2100 ggtgaatagg tttttttaac actattttat taaggaagag gatgaaaaat aaaaaaagtg 2160 gacaaatttc ttgttaattg taattacatg cattgcaatg gttttctttt ttacatcgtg 2220 tactattcag tctgctatag agcagaagaa aactgttgag gaaatcttgg gaaaaatagg 2280 tgagagtgag gacaaaacaa attcaagggg gcaaccagca acaatgaaag aggatgaagt 2340 tgaagataat cctttaaaag atgtatataa agattatttc ctggttggag cagcaattaa 2400 tggctattct gttgaaactg ctgctatcaa tcatcctggt atggctgcaa ttttgaaaaa 2460 aactttaaca gtacaaccct atctaatttg atgaaacaac aatacctttt agattatgaa 2520 gctacaaaag caagtaaaaa tggaatgcca gtgtgtaaat ttgacagctg cattcctgct 2580 ttacaatttt gtaaggaaaa tggcataaaa atgagaggac atgtgttagt atggcataat 2640 cagacaccag aatggttttt ccacaaagac tatgatgtat cgaaaccact tgtagatgct 2700 gctactatgg aacgccggtt ggaaagttat atcaaacagg taattgaatt ttgtcaaaaa 2760 aattatcccg gtgtagtcta ttgctgggat gttgttaacg aagctatact tgatgatggt 2820 tcatggagag aaatcaataa taattggtat accattatga aagaaaagta tgtggaaaag 2880 gcattttatt atgcaagaaa atatgccaaa aaagatgttg ccctgtttta caatgattac 2940 aatgtttttc tccctgcaaa gagagaagca atttataatc ttgctcagaa acttaaagaa 3000 aaaggattga ttgacgggtt gggtcttcaa cctacagtag gcttgaatta tcctgaatta 3060 gattctgatg atatagattc attcaaaacg acattagaaa catttgcaaa acttggctta 3120 caaattcata ttactgagtt aaattttgaa ataaagggag atgagagcaa tcgtactcct 3180 gaaaatctca aaaaacaagc agataggtat tacgaaatga tgaagttatt attgaaggaa 3240 gatactgata atggtgggcc ttgcaacata acttgtgtta ctgtttttgg tatctgtgac 3300 gattatccac tatataaaaa ttttaagcag tgcatgtatc tttgggataa aaattgcaat 3360 cctaaaccat gtttttattc atttctccaa gcaggtttag actggaaagc atctttatta 3420 agcaaataag aatgaacaac acttatggag aggaggaaaa taatgaaaat aactattaat 3480 tatggaaaga gacttgggaa aataaacaaa ttttgggcaa aatgtgttgg aagctgtcat 3540 gctacaactg cgttaagaga agactggcga aagcaattaa aaaaatgtcg tgacgaactt 3600 ggttttgagt atattcgatt tcatggttgg ttgaatgatg atatgagtgt ttgttttaga 3660 aatgatgatg ggctactttc attctcattc ttcaacatag attctataat tgattttctt 3720 ttggagatag gtatgaaacc atttattgaa ctgagcttta tgccagaagc gttagcgtca 3780 ggtacaaaga cagttttcca ttacaaagga aatataacac cgccgaaatc ttatgaagaa 3840 tggggtcagc tgattgagga gttagcaagg catcttatta gcagatatgg gaaaaatgaa 3900 gtaagagaat ggttttttga ggtatggaac gaaccaaatc taaaggattt cttctgggca 3960 ggaacaatgg aagaatattt taagctttac aaatatgctg cttttgcaat aaagaaagtg 4020 gactctgaac taagggtagg tggaccagct actgcaatcg atgcatggat acctgaacta 4080 aaagattttt gtacaaaaaa tggtgttcca atagatttta tttcaacgca tcaatatcca 4140 acagatttag cattcagtac aagctcaaat atggaagagg ctatggcaaa agcaaagaga 4200 ggtgaattag cagagagggt aaaaaaggct ttagaggaag catatccatt gcctgtttac 4260 tacactgaat ggaataactc tccaagtcct cgagacccat atcacgacat accttacgat 4320 gctgctttta ttgtaaaaac aataattgac attatagatt taccacttgg gtgttattct 4380 tattggacat ttacagatat ctttgaagaa tgtggacaga gttctttacc ttttcatggg 4440 ggattcgggc ttctaaatat tcatggtata ccaaaaccat cctatagagc atttcaaatt 4500 ttagataaac taaacggtga gaggattgag atagagtttg aagataaaag cccaaccatt 4560 gattgtatag ctgtccagaa tgagagagag ataatacttg tgatctcaaa ccataatgtt 4620 ccgctgtctc ctattgatac cgaaaatata aaagttgttt taaaaggtat tgagaattgc 4680 cgagaagttt ttgttgagag aatagatgaa tataatgcca atccaaaaag agtatggctt 4740 gaaatgggca gtcctgcgta tctcaataga gaacagattg aggagttgat aaaagcatca 4800 gaactaaaga aagagaaagt ttcatggggg attgtgaata ataatgaaat tacatttgat 4860 ttaagtgttt tacctcactc agttgtggct gttacaatta agaatggtta gtgaaatgtt 4920 aagagagaaa agcaattttg tatatctctt ttaattttta cctttgacac atcaaacaat 4980 ctaaattaaa attaaagtat agtgttttgc atactcaaca tagtataaat tatataaggg 5040 taacattaat accctttttg tttttgtaag ggggtgtttt tgtggcaaag cacacgcaaa 5100 aaggtaaatc agctgccaca gccgccgtgt cagacaaaga aaaagcaagg tttgttccta 5160 aaaatattca agctgagata aaagaaaaga ttaaagacac tggtgaaaaa gtagcaaagg 5220 ctgagggtaa ggacaaagca cttttacagt taaagctgga gagcaacaaa aaggttgata 5280 agaaaaaatt caaaaaggat agaagtgttg agaggaataa aacttcatta aatagatttt 5340 taagtttaga taaaattaaa tccctatatt caaaagagat acataataaa ctttcacaca 5400 tctttgaaga tgcagtttct gaggtttata gaattttaat ggggctaaag tatatcaaaa 5460 aggcgccaaa ttacaccgaa attgttctga aggcaaagat attttcaacc ttgattttga 5520 tgattgtaat attattttta atcaacaaaa tgccttctac atacaaaaaa gcgtatgcag 5580 ttgttttgaa caatcagatt gtagggtatg tgaaggacaa gactgaagca caaaaccttc 5640 ttacccagat taaaaaagaa gtagaggaaa gacacaatac agacagtttc attttacaaa 5700 gtaagcttca actaaagagc attgagcctg gtcaatatcg tgagacaagg gttgatgagc 5760 tgaaaaatac tatcatagaa aaggggaagg tccttgtaaa aaggtatgct atttttgtta 5820 attcaaaacc atattttgta tttgaaaatc cacaaactcc aaataatatt cttaacaagc 5880 taaaaaaggt ctattataat gacaaggcat cacaggcaaa attcttagag aaggtagaaa 5940 taaaaccagt ttatgtctca ccagctatta aagtagctga tgaagctact gccttaacaa 6000 agattatgtt tgggaaagac caggtaatag aatatacagt caaggaagga gatactcttt 6060 gggatcc 6067 // |
|