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EBI DbfetchID M34053; SV 1; linear; genomic DNA; STD; PRO; 4880 BP. XX AC M34053; XX DT 01-JUL-1990 (Rel. 24, Created) DT 17-APR-2005 (Rel. 83, Last updated, Version 5) XX DE B.subtilis folic acid biosynthetic operon encoding para-aminobenzoic acid DE synthase, component I (pab), glutamine amidotransferase (trpG), pot. DE p-aminobenzoate synthesis enzyme (pabC), and pot. dihydropteroate synthase DE (sul) genes, complete cds. XX KW dihydropteroate synthase; glutamine amido transferase; KW H2-pteridine-CH2OH pyrophosphokinase; para-aminobenzoic acid synthase. XX OS Bacillus subtilis OC Bacteria; Firmicutes; Bacillales; Bacillaceae; Bacillus. XX RN [1] RP 1-4880 RX PUBMED; 2123867. RA Slock J., Stahl D.P., Han C.-Y., Six E.W., Crawford I.P.; RT "An apparent Bacillus subtilis folic acid biosynthetic operon containing RT pab, an amphibolic trpG gene, a third gene required for synthesis of RT para-aminobenzoic acid, and the dihydropteroate synthase gene"; RL J. Bacteriol. 172(12):7211-7226(1990). XX DR GOA; P29252. DR HSSP; 1F9Y; P26281. DR InterPro; IPR000550; Hppk. DR UniProtKB/Swiss-Prot; P29252; HPPK_BACSU. XX CC Draft entry and computer-readable sequence for [Unpublished (1990)] CC kindly submitted CC by D.P.Stahly, 07-MAY-1990. CC Author address: D.P.Stahly CC University of Iowa CC Dept. of Microbiology CC Iowa City, IA 52242 CC e-mail: cmdsix@vaxa.weeg.uiowa.edu. XX FH Key Location/Qualifiers FH FT source 1..4880 FT /organism="Bacillus subtilis" FT /strain="W168" FT /sub_strain="PY79" FT /mol_type="genomic DNA" FT /db_xref="taxon:1423" FT RBS 300..303 FT /note="ribosome binding site" FT CDS 313..1725 FT /codon_start=1 FT /transl_table=11 FT /note="para-aminobenzoic acid synthase, component I (pab)" FT /db_xref="GOA:P28820" FT /db_xref="HSSP:1K0E" FT /db_xref="InterPro:IPR015890" FT /db_xref="UniProtKB/Swiss-Prot:P28820" FT /protein_id="AAA22694.1" FT /translation="MAQRRPAGKKIPFQKDSFLQQFEKLAQSRKHHVLLESARGGRYSI FT AGLDPIATVKGKDGITTIKHGDEMLFKEGDPLRAFHSWFKTLETETNHEFPDFQGGAIG FT FLSYDYARYIENFKMLSLDDLETPDIYFLVFDDIAVYDHQEESLWLITHVNGSDQETAD FT VKLSELEQMWLTELPAVTSREMKPETAGSFAAPFTEDGFSQAVEKIKQYIASGDVFQVN FT LSIRQSQSLSVHPYQIYKTLREVNPSPYMAYLETPDFQIICGSPELLVSKKGKLLETRP FT IAGTRSRGKTNEEDEALANELIHNEKERAEHVMLVDLERNDLGRVSRYGSVRVNEFMAI FT EKYSHVMHIVSNVQGELQDGYDAVDIIHAVFPGGTITGAPKVRTMEIIEELEPTRRGLY FT TGSIGWFGYNHDLQFNIVIRTIYATGGQAFMQSGAGVVIDSVPKHEYKESFKKAFAMQR FT ALELSEEETKIR" FT misc_binding 1720..1728 FT /bound_moiety="methyltryptophan resistance protein" FT RBS 1724..1730 FT /note="ribosome binding site" FT CDS 1739..2323 FT /codon_start=1 FT /transl_table=11 FT /note="glutamine amidotransferase (trpG)" FT /db_xref="GOA:P28819" FT /db_xref="HSSP:1I1Q" FT /db_xref="InterPro:IPR006221" FT /db_xref="UniProtKB/Swiss-Prot:P28819" FT /protein_id="AAA22695.1" FT /translation="MILMIDNYDSFTYNLVQYLGELGEELVVKRNDSITIDEIEELSPD FT FLMISPGPCSPDEAGISLEAIKHFAGKIPIFGVCLGHQSIAQVFGGDVVRAERLMHGKT FT SDIEHDGKTIFEGLKNPLVATRYHSLIVKPETLPSCFTVTAQTKEGEIMAIRHNDLPIE FT GVQFHPESIMTSFGKEMLRNFIETYRKEVIA" FT RBS 2306..2313 FT /note="ribosome binding site" FT CDS 2323..3204 FT /codon_start=1 FT /transl_table=11 FT /note="pot. p-aminobenzoate synthesis enzyme (pabC); FT putative" FT /db_xref="GOA:P28821" FT /db_xref="InterPro:IPR018300" FT /db_xref="UniProtKB/Swiss-Prot:P28821" FT /protein_id="AAA22696.1" FT /translation="MIYVNGRYMEEKDAVLSPFDHGFLYGIGVFETFRLYEGCPFLLDW FT HIERLERALKDLQIEYTVSKHEILEMLDKLLKLNDIKDGNARVRLNISAGISDKGFVAQ FT TYDKPTVLCFVNQLKPESLPLQKEGKVLSIRRNTPEGSFRLKSHHYLNNMYAKREIGND FT PRVEGIFLTEDGAVAEGIISNVFWRKGRCIYTPSLDTGILDGVTRRFIIENAKDIGLEL FT KTGRYELEALLTADEAWMTNSVLEIIPFTKIEEVNYGSQSGEATSALQLLYKKEIKNMI FT HEKGGRAWRSTQ" FT RBS 3175..3179 FT /note="ribosome binding site" FT CDS 3186..4043 FT /codon_start=1 FT /transl_table=11 FT /note="pot. dihydropteroate synthase (sul) (EC 2.5.1.15); FT putative" FT /db_xref="GOA:P28822" FT /db_xref="HSSP:1AD1" FT /db_xref="InterPro:IPR006390" FT /db_xref="UniProtKB/Swiss-Prot:P28822" FT /protein_id="AAA22697.1" FT /translation="MAQHTIDQTQVIHTKPSALSYKEKTLVMGILNVTPDSFSDGGKYD FT SLDKALLHAKEMIDDGAHIIDIGGESTRPGAECVSEDEEMSRVIPVIERITKELGVPIS FT VDTYKASVADEAVKAGASIINDIWGAKHDPKMASVAAEHNVPIVLMHNRPERNYNDLLP FT DMLSDLMESVKIAVEAGVDEKNIILDPGIGFPKTYHDNLAVMNKLEIFSGLGYPVLLAT FT SRKRFIGRVLDLPPEERAEGTGATVCLGIQKGCDIVRVHDVKQIARMAKMMDAMLNKGG FT VHHG" FT RBS 4023..4027 FT /note="ribosome binding site" FT CDS 4036..4398 FT /codon_start=1 FT /transl_table=11 FT /note="ORF1" FT /db_xref="GOA:P28823" FT /db_xref="HSSP:1DHN" FT /db_xref="InterPro:IPR006156" FT /db_xref="UniProtKB/Swiss-Prot:P28823" FT /protein_id="AAA22698.1" FT /translation="MDKVYVEGMEFYGYHGVFTEENKLGQRFKVDLTAELDLSKAGQTD FT DLEQTINYAELYHVCKDIVEGEPVKLVETLAERIAGTVLGKFQPVQQCTVKVIKPDPPI FT PGHYKSVAIEITRKKS" FT RBS 4381..4385 FT /note="ribosome binding site" XX SQ Sequence 4880 BP; 1512 A; 939 C; 1205 G; 1224 T; 0 other; gaattctcgg aggcatctca tcaggtgcgg caatttatgc tgctcttcaa gtagcaaaaa 60 aactcggaaa aggcaaaaaa gtattggcca tcattccaag taacggcgaa cgttatctaa 120 gcacgccgct gtaccaattc gattaaaaaa agccaaaact cccggttcgc cgggagtttt 180 tttatatttc gtgcatcaaa tatgccatgt ctgcgacgaa tgttattctt ggaagagaaa 240 ttcacttttt tcactaacaa cattgcttta caattaaaaa caagtaaaaa agaaaataga 300 ggaatgatac aaatggcaca acgcagaccg gcaggcaaaa aaataccttt tcaaaaagac 360 tcattcttac aacaatttga gaaacttgcg caatcccgga aacatcatgt acttctcgaa 420 agtgcaagag gcggcagata tagtatagcc ggtcttgatc caattgcgac tgtgaaagga 480 aaagacggaa taactacaat taagcatggt gatgagatgc tgtttaaaga aggtgatcca 540 ttacgggcct tccacagctg gtttaaaaca ctggaaacag aaacgaatca tgagttccct 600 gactttcaag gcggggcaat cgggtttctc agctatgatt acgcacggta cattgaaaat 660 tttaaaatgc tctcattaga tgatttagaa acaccagata tttattttct tgtttttgat 720 gatatagcag tttatgacca tcaagaagag tctctatggc tgattactca tgttaatggt 780 tctgatcagg aaacagcgga tgtgaagcta tctgagttag agcagatgtg gttgactgag 840 cttcccgctg tcacttcgcg agagatgaag cctgaaacag ctggttcttt cgcggcgcca 900 tttaccgagg atgggttctc acaagctgta gagaaaatca aacaatacat tgccagcgga 960 gatgtgtttc aagtcaatct atcaataagg cagtcacagt cactgtctgt ccacccatat 1020 caaatttaca aaaccttgag agaagtaaat ccttctcctt atatggcgta tttagaaaca 1080 cctgatttcc aaatcatttg cggatcgcct gaactgcttg tcagcaaaaa gggcaagcta 1140 ttagagacga gaccgattgc gggcacccgt tccagaggga aaacaaatga agaagacgag 1200 gcgcttgcaa acgaattgat acacaatgaa aaagaacgcg cggaacatgt catgctggtt 1260 gatcttgagc gaaatgatct gggaagagta tcacgttacg ggtctgtgcg cgtaaatgaa 1320 ttcatggcaa ttgaaaaata ctcgcatgtg atgcacattg tgtctaatgt ccaaggtgaa 1380 ctgcaggatg ggtatgatgc tgtagatatt attcatgctg tgtttcccgg aggaaccatt 1440 actggtgcac cgaaagtaag aacgatggaa attatagaag aacttgagcc gacacgccga 1500 gggctttata ctggatctat aggatggttt ggatataatc acgatctgca gtttaatatc 1560 gtcattcgaa ccatttatgc aaccggaggg caggcattta tgcagtccgg tgcaggagtt 1620 gtgattgatt ctgttccgaa gcacgaatac aaggaatcat tcaaaaaagc ttttgcgatg 1680 caaagagcat tagagctgag cgaagaagag acaaaaatta gatgaggtga gcggagaaat 1740 gattttaatg attgataact acgattcatt cacgtacaac ttggtacagt atttgggcga 1800 gcttggggaa gagctggttg tgaaacgcaa tgacagcatc acaatcgatg aaattgaaga 1860 actgtctccg gactttctga tgatatctcc cggaccgtgc agccctgatg aggcgggaat 1920 cagcctcgaa gcaattaaac atttcgcagg gaaaattcct attttcggtg tatgtctcgg 1980 acatcagtcc atcgcacaag tgttcggtgg tgatgttgtt agggcagaac ggcttatgca 2040 cgggaaaacc tcggatatcg agcatgacgg caaaaccatt tttgaagggt tgaaaaatcc 2100 ccttgttgcg acgcgatacc actcgctgat cgtaaaacct gagacgctgc caagctgttt 2160 tacagtaaca gcacaaacga aagaaggaga aatcatggct attcgccaca atgacctccc 2220 gatagagggt gtgcaatttc acccagagtc tattatgacc tcctttggga aagaaatgct 2280 cagaaatttt attgagacat atcgcaagga agttattgcg tgatgatata tgtgaacggc 2340 cggtatatgg aggagaaaga tgcagttctt tctccttttg accatgggtt tttatatggg 2400 atcggtgttt tcgaaacctt caggctctac gaaggctgcc cgtttttgct tgattggcat 2460 atagagaggc ttgaacgcgc gcttaaggat ctgcaaatcg aatatactgt ttcaaagcat 2520 gagatccttg agatgctgga caagctgctt aagctaaatg atatcaagga tggcaatgcc 2580 cgcgtcagac tgaacatatc agcgggaatc agcgataaag gctttgtcgc ccagacgtac 2640 gataagccca ccgttttatg ctttgtgaat cagctgaaac cagaaagtct tcctttgcaa 2700 aaagaaggaa aggttttatc gatacggaga aacactccag agggatcgtt ccggttaaaa 2760 tctcatcatt atctaaataa tatgtatgcg aaacgggaaa ttggaaatga tccgcgtgtc 2820 gagggaattt tcttaacgga ggatggagct gttgcagagg gtatcatatc taatgtcttc 2880 tggagaaaag gtcgctgtat ctatacgcca tcgcttgata ccggtattct ggacggcgtc 2940 acgagacgtt ttatcataga aaatgcaaag gatatcggcc tggagctcaa aacgggccga 3000 tatgagctag aagctctctt gacagctgac gaagcgtgga tgacgaactc ggttcttgaa 3060 atcatcccgt ttactaaaat agaagaagta aactatggaa gccaaagcgg agaagcaacc 3120 tccgctcttc aattgttata caaaaaagaa ataaagaaca tgattcatga aaagggagga 3180 agagcatggc gcagcacaca atagatcaaa cacaagtaat ccacactaag cccagcgctt 3240 tatcatataa agagaagacg ctggtgatgg gaattttaaa cgtaacgcct gactctttct 3300 cggacggcgg aaaatatgac agcttggaca aggcgctgct gcacgcgaaa gagatgatcg 3360 atgatggtgc ccatatcatt gatattggag gggaatcgac aaggcctggc gctgagtgcg 3420 tatctgagga tgaggagatg tccagagtca ttccggtgat tgagcggatt acgaaagagc 3480 ttggtgttcc tatttctgta gacacgtaca aggcttctgt cgcagatgaa gcagtgaaag 3540 ccggtgcatc cattatcaat gatatttggg gagccaaaca tgatccgaag atggcttccg 3600 ttgcagctga acataatgtt ccaattgtac tcatgcataa ccgccctgaa agaaactaca 3660 atgacttatt gccggatatg ctgtcggact taatggagag tgtaaaaatt gctgttgagg 3720 ccggagtaga cgagaagaac attattcttg atcctggtat cggtttcccg aaaacctatc 3780 acgataactt ggcagtgatg aacaaactag agattttcag cggattggga tatccggttc 3840 ttctggcaac ctcccgaaaa agattcatcg gacgtgttct ggatcttccg cctgaggagc 3900 gggctgaggg cacaggcgcg actgtgtgtc tcggcattca aaaaggctgt gacattgtca 3960 gggtccatga tgtaaagcaa attgccagaa tggcgaaaat gatggacgcg atgctgaata 4020 agggaggggt gcaccatgga taaagtttat gtagaaggta tggagtttta cggatatcac 4080 ggtgtgttca cagaagaaaa caaacttggc cagcggttta aagtcgattt aaccgctgag 4140 ctggatttaa gcaaagctgg acagacagac gaccttgagc aaacgatcaa ctatgctgag 4200 ctctatcacg tatgtaaaga tatcgtggaa ggggagcctg tgaaattggt ggaaacgctg 4260 gcggaacgta ttgctggcac tgttctcgga aaatttcagc ctgttcagca atgtacggtg 4320 aaagtgatta agccagaccc gccaattccc ggacactata aatcagtagc aattgaaatt 4380 acgagaaaaa agtcatgaac aacatagctt atattgcact tggatctaat attggagata 4440 gagaaacgta tttaaggcaa gcagtggctt tactgcatca gcatgctgcg gtgacagtca 4500 ctaaagtgtc gtctatttac gaaactgacc cggtcggata cgaagatcaa gctcaatttt 4560 tgaatatggc tgttgaaatc aagacatcat tgaacccttt tgaactcctt gaactgacgc 4620 agcagataga aaatgaatta ggcagaacaa gggaagtaag atggggaccg cggacggcag 4680 accttgacat tttgttattt aatcgtgaaa atattgaaac agagcaacta attgttccgc 4740 atccgagaat gtatgagcgt ttgtttgtcc ttgcgccgct tgcggaaatt tgccagcagg 4800 ttgaaaaaga ggctacaagc gccgaaacag accaagaagg tgtaagagta tggaagcaga 4860 aatctggggt agacgaattc 4880 // |
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