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EBI DbfetchID M33766; SV 1; linear; genomic DNA; STD; PRO; 2374 BP. XX AC M33766; XX DT 16-JUL-1990 (Rel. 24, Created) DT 17-APR-2005 (Rel. 83, Last updated, Version 6) XX DE E.coli (clone pLC20-5) ornithine decarboxylase (speC) and cyclic AMP DE receptor protein genes, complete cds. XX KW cAMP receptor protein (CRP); ornithine decarboxylase; speC gene. XX OS Escherichia coli OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacteriales; OC Enterobacteriaceae; Escherichia. XX RN [1] RP 1-2374 RA Barroso L.A., Moore R., Wright J., Patel T., Boyle S.M.; RT "Analysis and sequence of the speC (ornithine decarboxylase) gene of RT Escherichia coli"; RL Unpublished. XX RN [2] RX DOI; 10.1073/pnas.89.10.4529 RX PUBMED; 1584788. RA Kashiwagi K., Miyamoto S., Suzuki F., Kobayashi H., Igarashi K.; RT "Excretion of putrescine by the putrescine-ornithine antiporter encoded by RT the potE gene of Escherichia coli"; RL Proc. Natl. Acad. Sci. U.S.A. 89(10):4529-4533(1992). XX CC On May 11, 1995 this sequence version replaced gi:147860. CC Draft entry and computer-readable sequence for [1] kindly submitted CC by S.M.Boyle, 22-MAY-1990. CC Author address: S.M.Boyle CC Virginia Polytechnic Institute and State University CC College of Veterinary Medicine, VPI & SU CC Blacksburg, VA 24061. XX FH Key Location/Qualifiers FH FT source 1..2374 FT /organism="Escherichia coli" FT /strain="K-12" FT /mol_type="genomic DNA" FT /db_xref="taxon:562" FT -10_signal 14..19 FT mRNA 15..2289 FT /note="speC mRNA (alt., primer extension)" FT mRNA 16..2289 FT /note="speC mRNA (alt., S1 mapping)" FT mRNA 27..2289 FT /note="speC mRNA (alt., primer extension)" FT mRNA 30..2289 FT /note="speC mRNA (alt., S1 mapping)" FT mRNA 41..2289 FT /note="speC mRNA (alt., S1 mapping)" FT mRNA 42..2289 FT /note="speC mRNA (alt., primer extension)" FT misc_binding 66..72 FT /bound_moiety="GTP phosphoryl binding site" FT CDS 86..2281 FT /codon_start=1 FT /transl_table=11 FT /note="ornithine decarboxylase (speC) (EC 4.1.1.17)" FT /db_xref="GOA:P21169" FT /db_xref="HSSP:1C4K" FT /db_xref="InterPro:IPR015422" FT /db_xref="UniProtKB/Swiss-Prot:P21169" FT /protein_id="AAA66174.1" FT /translation="MGQGFPPCPVFLLPRNGFALMKSMNIAASSELVSRLSSHRRVVAL FT GDTDFTDVAAVVITAADSRSGILALLKRTGFHLPVFLYSEHAVELPAGVTAVINGNEQQ FT WLELESAACQYEENLLPPFYDTLTQYVEMGNSTFACPGHQHGAFFKKHPAGRHFYDFFG FT ENVFRADMCNADVKLGDLLIHEGSAKDAQKFAAKVFHADKTYFVLNGTSAANKVVTNAL FT LTRGDLVLFDRNNHKSNHHGALIQAGATPVYLEASRNPFGFIGGIDAHCFNEEYLRQQI FT RDVAPEKADLPRPYRLAIIQLGTYDGTVYNARQVIDTVGHLCDYILFDSAWVGYEQFIP FT MMADSSPLLLELNENDPGIFVTQSVHKQQAGFSQTSQIHKKDNHIRGQARFCPHKRLNN FT AFMLHASTSPFYPLFAALDVNAKIHEGESGRRLWAECVEIGIEARKAILARCKLFRPFI FT PPVVDGKLWQDYPTSVLASDRRFFSFEPGAKWHGFEGYAADQYFVDPCKLLLTTPGIDA FT ETGEYSDFGVPATILAHYLRENGIVPEKCDLNSILFLLTPAESHEKLAQLVAMLAQFEQ FT HIEDDSPLVEVLPSVYNKYPVRYRDYTLRQLCQEMHDLYVSFDVKDLQKAMFRQQSFPS FT VVMNPQDAHSAYIRGDVELVRIRDAEGRIAAEGALPYPPGVLCVVPGEVWGGAVQRYFL FT ALEEGVNLLPGFSPELQGVYSETDADGVKRLYGYVLK" FT misc_binding 263..266 FT /bound_moiety="GTP phosphoryl binding site" FT misc_binding 364..367 FT /bound_moiety="pyridoxal-PO4 binding site" FT misc_binding 379..382 FT /bound_moiety="GTP guanine binding site" FT CDS complement(630..719) FT /codon_start=1 FT /transl_table=11 FT /note="cyclic AMP receptor protein (CRP)" FT /db_xref="GOA:Q47650" FT /db_xref="UniProtKB/TrEMBL:Q47650" FT /protein_id="AAA66175.1" FT /translation="MCRSAQNRFYRHERLWLRISAHLSPILHE" FT -10_signal complement(728..733) FT -35_signal complement(750..755) FT misc_signal 2289..2320 FT /note="rho-independent transcription termination signal" XX SQ Sequence 2374 BP; 515 A; 556 C; 678 G; 625 T; 0 other; ctgcaggaca acagtaaaat cagagcgttt ctgcttttac tgatgtctgg cggtcggagc 60 tggtgaccag tttgacccat atctcatggg gcagggtttt ccaccttgtc cggtattctt 120 acttccccga aacgggtttg cgcttatgaa atcaatgaat attgccgcca gtagtgaact 180 ggtatcccga ctttcttctc atcgtcgcgt ggtggcgttg ggagatactg attttacgga 240 cgtcgcggca gtcgtcatta ccgctgcgga tagtcgcagt ggcattcttg cgttgcttaa 300 gcgcaccggt tttcatctac cggtgttttt gtattccgaa catgctgttg aattacctgc 360 gggcgttacg gcggtaatca acggcaacga gcagcagtgg ctggagctgg aatccgcagc 420 ctgtcagtat gaagagaatt tgctgccacc gttttatgac acgctgacgc agtacgttga 480 gatgggcaac agcacctttg cttgccctgg acatcaacat ggtgcgtttt ttaaaaagca 540 tcctgccgga cgccattttt acgatttctt tggtgagaac gtctttcgcg ccgatatgtg 600 taacgctgac gtaaaattgg gcgatctgct tattcatgaa ggatcggcga aagatgcgca 660 gaaattcgca gccaaagtct ttcatgccga taaaacctat tttgtgctga acggcacatc 720 ggcagcgaat aaagtggtga cgaatgcgct gttaacgcgt ggcgatctgg tgctcttcga 780 ccgtaacaac cataagtcga atcatcacgg cgcgctgatt caggcggggg cgacgccggt 840 ctatctggaa gcttcacgca acccgtttgg tttcattggc ggtattgatg cgcactgttt 900 taatgaagag tatctgcgcc agcaaattcg cgacgttgcg ccagaaaaag ccgacctgcc 960 gcgcccgtat cgcctggcga ttattcagct gggaacctat gacggcactg tctataacgc 1020 ccgtcaggtg atcgataccg ttgggcatct gtgtgattac attctgtttg attccgcgtg 1080 ggtcggttat gaacaattta tcccgatgat ggcggatagc tcgccgctgc tgttagaact 1140 taacgaaaac gatccgggga tctttgtgac tcagtcggtg cacaaacagc aggcgggatt 1200 ctcacagacg tcgcagatcc ataaaaaaga taaccatatc cgcggacagg cgcgtttttg 1260 cccgcataag cggttgaata acgcctttat gctccatgct tctaccagcc ctttctatcc 1320 gctgtttgct gcactggatg ttaacgccaa aattcatgaa ggggagagtg ggcgtcggct 1380 gtgggctgag tgtgttgaga tagggattga agcgcgcaag gctattcttg cgcgctgtaa 1440 gctgttccgc ccgtttatcc cgcccgttgt tgatggcaaa ttgtggcagg attatccgac 1500 atcagtgtta gccagcgacc gccgtttttt cagttttgag ccgggggcga agtggcacgg 1560 ctttgaagga tatgccgcgg atcagtattt tgttgatccg tgcaagctgt tactcactac 1620 accaggtatc gatgccgaaa ccggcgaata tagcgacttt ggcgttccgg cgacgattct 1680 ggcgcactat ctgcgtgaga acggcattgt gccggagaag tgcgatctca actccattct 1740 gtttttatta actccggcgg aaagccacga gaagctggca caactggtgg cgatgctggc 1800 gcaatttgaa cagcatattg aggatgactc gccgctggtt gaggtgttgc cgagcgttta 1860 taacaagtat ccggtgcgct atcgcgacta caccctgcgc cagttgtgtc aggagatgca 1920 cgatctgtat gtcagtttcg acgtcaaaga cctacaaaaa gcgatgttcc gccagcagag 1980 tttcccgtca gtggtgatga acccccagga tgcgcatagc gcttatattc gcggtgacgt 2040 ggagttggtg cggattcgtg atgccgaagg gcgaattgcg gcagaagggg cgttgcctta 2100 tccacctggc gtgctttgcg tggtacccgg ggaagtctgg ggtggggcgg ttcaacgtta 2160 tttccttgca ctggaagaag gggtgaattt gttgccggga ttttcgccgg agctgcaagg 2220 tgtttatagc gaaaccgatg cggatggcgt gaaacggttg tacggttatg tgttgaagta 2280 agaataaaaa aaacgggtca ccttctggcg acccgttttt ctttgcgtaa ttagtggcta 2340 accgtctgtg tgcctgtcgg gacacgaacg tgtt 2374 // |
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