 |
|
EBI DbfetchID M33692; SV 1; linear; genomic DNA; STD; PRO; 1986 BP. XX AC M33692; XX DT 14-AUG-1990 (Rel. 25, Created) DT 17-APR-2005 (Rel. 83, Last updated, Version 5) XX DE P.solanacearum polygalacturonase A (PGA, pglA) gene, complete cds. XX KW alpha-1,4-endopolygalacturonase A. XX OS Ralstonia solanacearum OC Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales; OC Burkholderiaceae; Ralstonia. XX RN [1] RP 1-1986 RA Huang J., Schell M.A.; RT ; RL Unpublished. XX RN [2] RP 1-1715 RX PUBMED; 2193922. RA Huang J., Schell M.A.; RT "DNA sequence analysis of pglA and mechanism of export of its RT polygalacturonase product from Pseudomonas solanacearum"; RL J. Bacteriol. 172(7):3879-3887(1990). XX CC Draft entry and computer-readable sequence for [1] kindly submitted CC by M.A.Schell, 13-APR-1990, for release after publication. XX FH Key Location/Qualifiers FH FT source 1..1986 FT /organism="Ralstonia solanacearum" FT /mol_type="genomic DNA" FT /db_xref="taxon:305" FT misc_signal 27..59 FT /note="promoter region" FT misc_signal 27..31 FT /note="-30 Pseudomonas promoter consensus; putative" FT mRNA 58..>1986 FT /note="pglA mRNA" FT RBS 89..92 FT /note="pglA ribosomal binding site (put.); putative" FT sig_peptide 100..162 FT /note="polygalacturonase A signal peptide" FT CDS 100..1689 FT /codon_start=1 FT /transl_table=11 FT /note="polygalacturonase A precursor (pglA, ttg start FT codon)" FT /db_xref="GOA:P20041" FT /db_xref="InterPro:IPR000743" FT /db_xref="InterPro:IPR011050" FT /db_xref="InterPro:IPR012334" FT /db_xref="UniProtKB/Swiss-Prot:P20041" FT /protein_id="AAA25931.1" FT /translation="MNHRYTLLALAAAALSAGAHATGTSVTAPWGEVAEPSLPADSAVC FT KTLSASITPIKGSVDSVDGNPANSQPDASRIQSAIDNCPAGQAVKLVKGSAGESGFLSG FT SLKLKSGVTLWIDTGVTLFASRNPADYDNGLGTCGTATTSNDKSCNALIVARDTAGSGI FT VGAGAIDGRGGSLVTSGPNANRLTWWDIAYLNKTKGLNQQNPRLIQTYNGSAFTLYGVT FT VQNSPNFHIVTTGTSGVTAWGIKIVTPSLAYAVAGYKCPSGSTPDKVTPATCFTPETVK FT NTDGFDPGQSTNVVLAYSYINTGDDHVAVKASSGPTRNLLFAHNHFYYGHGLSIGSETN FT TGVSNMLVTDLTMDGNDSSAGNGLRIKSDASRGGKVTNIVYDGICMRNVKEPLVFDPFY FT SSVKGSLYPNFTNIVVKNFHDLGSAKSIKRTMTFLGYKANKQKNPLTITLDNVVFDGTL FT PAFEGSHYGGPASPNGVHFTFGGTGPVSFADAIVTSSTTDVTVTGTPGTAAAVDCSKAF FT VPLKSVAPTSPI" FT mat_peptide 163..1686 FT /note="polygalacturonase A" XX SQ Sequence 1986 BP; 351 A; 685 C; 616 G; 334 T; 0 other; ctgcagcacg cccttcgcgc agacgaaggc gtgccgctgc ttcgctgatc ccggcgcatt 60 gcgtcctcac aaaagacctc tcttttcgga gaattgacct tgaatcaccg ctacacactc 120 ctcgcactcg ccgccgcagc cctgtctgcc ggagcgcacg caaccggtac gtccgtcacg 180 gccccgtggg gcgaggtggc cgagcccagc ctgccggcgg attcggcggt ctgcaagacg 240 ctgtcggcct cgatcacgcc gatcaaaggc tccgtcgatt cggtggacgg caaccccgcc 300 aactcccagc cggacgcgag ccgcatccag tccgccatcg acaactgccc ggccggccag 360 gccgtcaagc tcgtgaaggg cagcgccggc gaatccggct tcctgagcgg ctcgctgaag 420 ctcaagtcgg gcgtgacgct gtggatcgac accggcgtga ccctgttcgc ctcgcgcaac 480 ccggccgact acgacaatgg cctgggcacc tgcggcaccg ccaccaccag caacgacaag 540 tcgtgcaacg cgctgatcgt ggcgcgcgat acggcgggca gcggcatcgt cggcgccggc 600 gccatcgatg gccgcggcgg cagcctggtc accagcggcc cgaacgcgaa ccggctcacc 660 tggtgggaca tcgcttacct gaacaagacc aaggggctga accagcagaa cccgcgcctg 720 atccagacgt ataacggcag cgcgttcacg ctgtacggcg tgacagtgca gaactcgccc 780 aacttccata tcgtcaccac cggcacgtcg ggcgtaacgg catggggcat caagatcgtg 840 acgccgagcc tggcctacgc cgtggcgggc tacaagtgcc cgtccggctc cacgcccgac 900 aaggtcacgc ccgcgacctg cttcacgccc gagacggtca agaacaccga cggcttcgac 960 ccggggcaat cgaccaacgt ggtgctggcc tattcctaca tcaacacggg tgacgaccac 1020 gtcgcggtca aggccagcag cggcccgacg cgcaacctgc tcttcgcgca caaccatttc 1080 tactatgggc acggcctgtc gatcggcagc gaaaccaata ccggcgtgag caacatgctg 1140 gtgaccgacc tgacgatgga cggcaacgac agctcggccg gcaacggcct gcgcatcaag 1200 tccgatgcct cgcgcggcgg caaggtcacc aacatcgtct acgacggcat ctgcatgcgc 1260 aacgtcaagg agccgctggt gttcgacccc ttctacagca gcgtgaaggg ttcgctctat 1320 ccgaacttca ccaacatcgt cgtcaagaac ttccatgacc tgggcagcgc caagtccatc 1380 aagcgcacga tgacgttcct gggctacaaa gccaacaagc agaagaaccc gctgacgatc 1440 acgctcgaca acgtggtctt cgacggcacg ctgccggcct tcgaggggtc ccactacggc 1500 gggccggctt cgcccaacgg ggtgcacttc accttcggcg gcaccggtcc ggtgagcttt 1560 gccgacgcca tcgtcacgtc gtcgaccacc gatgtgacgg tgaccggcac gccgggcacg 1620 gccgcggcgg tggattgcag caaggccttc gtgccgctga agtccgtggc accgacctcg 1680 ccgatctgat cgggcgactc cgctcagcgg tacccgttct gccgcttgta ctggcggatg 1740 gcttcgtcgt ggttggggtc ctcgtgtgca ttgtgcgcgc gctgatcttc gaactcacgg 1800 atggcattgg cctgagcctc cgtcagtttg ggcagcggcg gcttgcgttg gggtgacggg 1860 gtggtgctgt acgtcaccgg ctggctgtcg gcggagttcg tcttcgtagg cgatgtggtg 1920 tccgcccttt tcaccgtggt agtcgttgcg cgcttcggtc tcgcggtcca gttgggtccg 1980 ctgcag 1986 // |
|