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EBI DbfetchID M32470; SV 1; linear; genomic DNA; STD; PRO; 3360 BP. XX AC M32470; XX DT 22-NOV-1990 (Rel. 25, Created) DT 09-SEP-2004 (Rel. 81, Last updated, Version 5) XX DE S.aureus Sau3AI-restriction-enzyme and Sau3AI-modification-enzyme genes, DE complete cds. XX KW Sau3AI-modification-enzyme; Sau3AI-restriction-enzyme. XX OS Staphylococcus aureus OC Bacteria; Firmicutes; Bacillales; Staphylococcus. XX RN [1] RP 1-3360 RX DOI; 10.1016/0378-1119(90)90465-4 RX PUBMED; 2227451. RA Seeber S., Kessler C., Goetz F.; RT "Cloning, expression and characterization of the Sau3AI restriction and RT modification genes in Staphylococcus carnosus TM300"; RL Gene 94(1):37-43(1990). XX CC Draft entry and computer-readable sequence [Unpublished (1990) CC Univ. of Tuebingen, D-7400 Tuebingen 1] kindly submitted by S.t CC Seeber, 02-MAR-1990. XX FH Key Location/Qualifiers FH FT source 1..3360 FT /organism="Staphylococcus aureus" FT /mol_type="genomic DNA" FT /db_xref="taxon:1280" FT RBS 437..441 FT /note="ribosome binding site" FT CDS 448..1917 FT /codon_start=1 FT /transl_table=11 FT /note="Sau3AIR protein (ttg start codon)" FT /db_xref="GOA:P16667" FT /db_xref="InterPro:IPR011337" FT /db_xref="PDB:2REU" FT /db_xref="UniProtKB/Swiss-Prot:P16667" FT /protein_id="AAA26672.1" FT /translation="MESYLTKQAVHNRAKEAVGKSVLELNGGESIKQSKSSVGDAFENW FT FGKKKDSDSKPDMAEAGVELKATPFKKLKNGKYSSKERLVLNIINYEKVANENFETSSF FT LSKNNTIELAFYEYIKGTPSDNWIIKEAVLYEMHKNPIDYEIIKQDWEIINQYINEGKA FT HELSEGLTSYLAPCTKGANASSLRNQPYSDIKAKQRAFSLKSGYMTSILRKYVLGDEKI FT DSIVKDPFEIKEKSIEDIVFEKFQPYINWSIDKLCEHFSINKGEKGLNYRIASAILNLK FT GKTTKSKPFPEVEEFEKSSIVVKTVHFNKKNVNKESMSFGAFKFEELANEEWEDSEGYP FT SAQWRNFLLETRFLFFVVKEDEDGVDIFKGIKFFSMPEEDINGPVKRMWDDTVKKLKEG FT VTLEAVPDKSTKDGWRIKNNFVDKSDDLICHVRPHTNNRDYRGGSNADKLPKKINWINR FT PDSDDYSDEWMTKQSFWINNDYIKKQVEDLL" FT RBS 2004..2010 FT /note="ribosome binding site" FT CDS 2017..3255 FT /codon_start=1 FT /transl_table=11 FT /note="Sau3AIM protein" FT /db_xref="GOA:P16668" FT /db_xref="HSSP:10MH" FT /db_xref="InterPro:IPR018117" FT /db_xref="UniProtKB/Swiss-Prot:P16668" FT /protein_id="AAA26673.1" FT /translation="MNKIKVVELFAGVGGFRLGLENTKNGIFDITWANQWEPSRKIQHA FT FDCYSKRFKNGIHSNKDIAQVSDEEMANTEADMIVGGFPCQDYSVARSLNGELGIQGKK FT GVLFWQIIRYIQNTFPKYLLLENVDRLLKSPSSQRGRDFAVMLSTLNELGYNVEWRVIN FT AADYGNAQRRRRVFIFGYKQDLNYSKAMEESPLDKIIYHNGLFAEAFPIEDYANKNRVN FT RTHITHDIVDISDNFSFQFYNSGIMKNGEILTIDTIPKYEKSVTLGEIIESNVDDGFSL FT NQDQIDKFKYLRGPKKIKRTTKDGHEYYFSEGGMSETDSLELPARTMLTSESSINRSTH FT FLNVDGVYRTLTPIEAERLNGFPDNWTEGMPIKMRYFCMGNALVVPLITRIGNQIEKID FT SITNDEFSQLRLF" XX SQ Sequence 3360 BP; 1295 A; 393 C; 638 G; 1034 T; 0 other; aagtcgacct tcaccaagac cgaattttcc gcgtataccc gcagtaccaa acgttaattt 60 actttcaaaa ccttctcgct gttcaatgtc agattgctgc tcataaaaat gtttaactaa 120 actatcatta gctctttcta tccataattc tttatccatt gttgctaaac atcctttcaa 180 aatctcagtt agacttaata aaacatgaaa actaaagccc ttacatttat gtaatgaatt 240 ataaagaaat acgccccaaa agtaaaaaaa cacagcccca agacaatact tttcacaagt 300 attatataat agatgtgtat gaaaatgcat ggagtagatg taagagtgat attcaaaatg 360 tgtaaaaaat atggataatt ctatataatt atattattga aattttaaat agcggaaata 420 attatttaat gttaagaggg gataattttg gaaagttatt tgacaaaaca agccgtacat 480 aatcgcgcaa aagaagctgt tggtaaaagt gtattagaat taaatggtgg tgaatcgatt 540 aaacaaagta agagttcagt tggtgatgca tttgaaaatt ggtttggtaa gaaaaaagac 600 agtgatagta aaccagatat ggcagaagct ggggtggaac ttaaggcaac gccatttaaa 660 aagttgaaaa acggaaagta tagctccaaa gaaagattag tattaaatat tataaactat 720 gagaaagtgg caaatgaaaa ttttgaaact agtagttttt tatctaagaa taatactata 780 gaattagctt tctatgaata tatcaaggga acacctagtg ataattggat tattaaagaa 840 gcggtgcttt atgaaatgca taaaaacccg attgattatg aaataattaa acaagattgg 900 gaaataataa atcaatatat taatgaagga aaggcacatg aattgagtga aggtttgaca 960 agttatttag cgccatgtac aaagggtgcg aatgctagtt ctttaagaaa tcagccttat 1020 tcagacataa aagcaaagca aagagcattt tctttgaagt ctgggtatat gacatctatt 1080 ttacgcaaat atgttctagg tgatgaaaaa atagattcaa ttgtcaaaga cccatttgaa 1140 ataaaagaaa aatcaataga ggacatagtc tttgaaaaat ttcagccata tataaactgg 1200 tcaatcgata aattatgcga acatttttct atcaataaag gtgagaaagg tttaaattat 1260 agaatagcct ctgccatttt aaatctaaaa ggtaaaacta ctaaaagtaa accattcccg 1320 gaagttgaag agtttgaaaa atcatctata gtagtcaaaa cagttcattt taataaaaag 1380 aatgtgaata aagaaagtat gtcatttgga gcttttaaat ttgaagaact agctaatgag 1440 gaatgggaag atagtgaagg atatcctagt gcacaatggc gaaacttttt gttagaaaca 1500 aggtttttat tttttgttgt taaagaagat gaagatggtg tagacatatt caaaggaata 1560 aaatttttta gtatgcctga agaagacata aacggacctg tcaaaagaat gtgggatgat 1620 acagtgaaga aattaaaaga gggtgtcaca ttagaagctg taccggacaa aagtacaaag 1680 gatggttgga gaataaaaaa taattttgta gataaaagtg atgatttaat ttgccatgtt 1740 agaccacaca ctaataacag agactatcgt ggaggaagta atgcagataa gcttcctaaa 1800 aagattaact ggattaatag acctgactca gatgattatt cggatgagtg gatgactaaa 1860 caaagttttt ggataaataa tgactacata aaaaagcaag ttgaagattt attgtagtta 1920 aagtatgtta aaatataaga tattctttta aaatatcgaa cgatcgttcg tattttgtgt 1980 tataataagg ttgaattaag tataggaggt cgcctaatga ataaaattaa agtagtagaa 2040 ttgtttgcgg gtgtaggcgg gtttcgttta ggtttagaaa atacgaaaaa tggtatattt 2100 gacataactt gggcaaatca atgggagccc tcacgaaaaa tccaacatgc atttgattgt 2160 tatagtaaaa gatttaagaa cggcatccat agtaataagg atattgccca ggtatctgat 2220 gaagaaatgg caaatactga agctgatatg attgtaggag gatttccttg ccaagattat 2280 tcagttgcaa ggagtttaaa tggagaatta ggaatacaag gaaaaaaggg cgttttattc 2340 tggcaaatta ttagatatat tcaaaataca tttcctaaat acttgttgct tgaaaatgtt 2400 gatagattat tgaaatcacc ttcgagtcag agagggagag actttgctgt aatgttatca 2460 accttaaatg agttaggcta taatgttgaa tggcgcgtga ttaatgctgc tgattatggc 2520 aatgctcaaa gacgtagaag ggtatttata tttggatata agcaagattt aaactatagc 2580 aaagctatgg aagaaagtcc gttggataaa attatatatc acaatggttt gtttgctgaa 2640 gcttttccga ttgaagatta tgccaataaa aatagagtaa ataggactca tattactcat 2700 gatatagtcg atatttcaga taatttcagt tttcaatttt ataatagtgg aatcatgaaa 2760 aatggagaaa ttttaactat tgacacaata ccaaaatatg aaaaatcagt aaccttagga 2820 gaaattattg aaagtaatgt agatgatggt ttttcattaa atcaagatca aattgataaa 2880 tttaaatatt taagaggacc caaaaagatt aaacgaacta ctaaagatgg tcatgaatat 2940 tatttttcag aaggtggtat gtctgaaaca gattcattag agttacctgc aagaacaatg 3000 cttacaagtg aatcatctat taatagaagt actcattttt taaacgtaga tggtgtttat 3060 agaactttga cacctattga agcagaaagg ttgaatgggt ttccagataa ttggacagaa 3120 ggtatgccaa ttaagatgag atacttttgt atgggcaatg ctcttgttgt gcctttgatt 3180 actagaatag gtaatcaaat tgaaaaaatt gatagtatta caaatgatga attcagtcag 3240 ctacgtttat tttaaataaa tcaaatgtag agtgcgtttg atttaacaag tttgtatact 3300 aaagattcat agattattgt atattgacca gtatttatca gcgtattatt ttaatatata 3360 // |
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